FastQCFastQC Report
Thu 26 May 2016
SRR937224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202696
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.5180171290997355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.46621541618976203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.2708489560721475No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.1045901251134704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGT250.00501656356.9964456
GGTATCA5050.051.7677651
AGCGTCG308.2842904E-539.58086826-27
GTATCAA8350.037.570381
GTGTTAT550.002566082934.568891
GTAGCGT352.0502604E-433.9306424-25
CGTCGTG352.0517473E-433.92645628-29
CCTAGAC600.00394454631.6646962
TAGGACC650.005834643729.2289494
TCAACGC10800.029.0259674
ATCAACG10900.028.7596743
TATCAAC10800.028.5861782
TGGTATC2550.027.9394362
CAACGCA11250.027.864935
AACGCAG11450.027.3782066
AGTAGCG350.00837773427.14786122-23
GCGTCGT350.00838782527.14116526-27
GTAAGGT1257.3552364E-626.5983434
TCGTGGT458.940767E-426.38724530-31
GTGTAAG1000.001642465723.766111
Produced by FastQC (version 0.11.5)