##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937224_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 202696 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.199466195682206 33.0 31.0 34.0 31.0 34.0 2 32.4085773769586 34.0 31.0 34.0 31.0 34.0 3 32.467715199115915 34.0 31.0 34.0 31.0 34.0 4 36.117086671665945 37.0 35.0 37.0 35.0 37.0 5 36.03448020681217 37.0 35.0 37.0 35.0 37.0 6 36.077835773769586 37.0 35.0 37.0 35.0 37.0 7 36.028199865808894 37.0 35.0 37.0 35.0 37.0 8 35.994469550459804 37.0 35.0 37.0 35.0 37.0 9 37.707502861427955 39.0 37.0 39.0 35.0 39.0 10-11 37.626470181947354 39.0 37.0 39.0 35.0 39.0 12-13 37.529016359474284 39.0 37.0 39.0 35.0 39.0 14-15 38.738110273513044 40.0 38.0 41.0 35.0 41.0 16-17 38.69609661759482 40.0 38.0 41.0 34.5 41.0 18-19 38.70813681572404 40.0 38.0 41.0 34.5 41.0 20-21 38.612885799423765 40.0 38.0 41.0 34.0 41.0 22-23 38.460850238781234 40.0 38.0 41.0 34.0 41.0 24-25 38.39963048111458 40.0 38.0 41.0 34.0 41.0 26-27 38.35582103248214 40.0 38.0 41.0 34.0 41.0 28-29 38.22693590401389 40.0 38.0 41.0 34.0 41.0 30-31 38.297201227453925 40.0 38.0 41.0 34.0 41.0 32-33 38.22734045072424 40.0 38.0 41.0 34.0 41.0 34-35 38.08747335911907 40.0 37.5 41.0 33.5 41.0 36-37 37.90531140229704 40.0 37.0 41.0 33.0 41.0 38-39 37.76826380392312 40.0 37.0 41.0 33.0 41.0 40-41 37.595749299443504 40.0 37.0 41.0 33.0 41.0 42-43 37.38639391009196 40.0 37.0 41.0 32.5 41.0 44-45 37.09916081225086 40.0 36.5 41.0 31.5 41.0 46-47 36.75619400481509 40.0 36.0 41.0 31.0 41.0 48-49 36.416757114101905 39.0 36.0 41.0 30.0 41.0 50-51 36.16702845640762 39.0 36.0 41.0 30.0 41.0 52-53 35.94645922958519 39.0 35.0 40.5 29.0 41.0 54-55 35.72153372538185 39.0 35.0 40.0 28.5 41.0 56-57 35.44657516675218 38.5 35.0 40.0 28.0 41.0 58-59 35.2001593519359 38.0 34.5 40.0 27.5 41.0 60-61 34.867570154319765 38.0 34.0 40.0 26.5 41.0 62-63 34.51571071950113 38.0 34.0 40.0 26.0 41.0 64-65 34.12260725421321 37.0 34.0 40.0 26.0 41.0 66-67 33.71737232111142 36.5 33.0 39.0 25.0 40.5 68-69 33.27527676915183 36.0 33.0 39.0 23.5 40.0 70-71 32.8694991514386 35.5 32.0 39.0 23.0 40.0 72-73 32.41745027035561 35.0 32.0 38.0 22.0 40.0 74-75 31.935067786241465 35.0 31.0 37.0 20.5 39.0 76-77 30.66029423372933 34.0 29.5 36.0 20.0 38.5 78-79 31.273826321190356 34.5 31.0 36.0 20.0 39.0 80-81 31.080378991198643 34.0 31.0 36.0 19.5 37.5 82-83 30.72655849153412 34.0 31.0 35.5 18.0 37.0 84-85 30.350848068042787 34.0 30.5 35.0 15.5 37.0 86-87 29.913446737972137 34.0 30.0 35.0 9.0 36.0 88-89 29.437911946955047 34.0 29.5 35.0 6.0 36.0 90-91 28.90578748470616 34.0 29.0 35.0 2.0 35.5 92-93 28.523656115562222 34.0 29.0 35.0 2.0 35.0 94-95 28.05121462683033 33.0 28.5 35.0 2.0 35.0 96-97 27.560302127323677 33.0 27.0 35.0 2.0 35.0 98-99 26.743376780992225 33.0 25.5 35.0 2.0 35.0 100-101 24.168543039823184 30.5 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07803034995400537 1101 2 -0.19967663826231075 1101 3 -0.12692516659323871 1101 4 -0.055110968425942986 1101 5 -0.3365679629122482 1101 6 -0.26472046162872687 1101 7 -0.02629663970394347 1101 8 -0.20489132551427502 1101 9 -0.1250779222072964 1101 10-11 -0.19930317561539823 1101 12-13 -0.17613319395316296 1101 14-15 -0.22457296807398563 1101 16-17 -0.2207082661258042 1101 18-19 -0.0029948590762955973 1101 20-21 -0.05910201497247414 1101 22-23 0.06436588670533183 1101 24-25 0.0741727561812624 1101 26-27 -0.30330080715388874 1101 28-29 -0.4604521807853672 1101 30-31 -0.49328515233382575 1101 32-33 -0.3240110162975256 1101 34-35 -0.3359461652652058 1101 36-37 -0.17048351681653173 1101 38-39 -0.5181379175070617 1101 40-41 -0.49456502330627927 1101 42-43 -0.45650872824849387 1101 44-45 -0.2135399027988214 1101 46-47 -0.8591350082350075 1101 48-49 -0.7003847186952541 1101 50-51 -0.8482161020262922 1101 52-53 -0.8637271020132573 1101 54-55 -0.36090975869778674 1101 56-57 -0.6398711017348617 1101 58-59 -0.48644684197842025 1101 60-61 -0.6960881341334826 1101 62-63 -0.6093514478028013 1101 64-65 -0.9226807885776438 1101 66-67 -0.6831019303785197 1101 68-69 -0.611168633742075 1101 70-71 -0.6867819730967142 1101 72-73 -0.6888827365104433 1101 74-75 -0.8340033320325801 1101 76-77 -0.6981485328956971 1101 78-79 -1.3817319903422067 1101 80-81 -1.2746634291834376 1101 82-83 -1.1188070275331228 1101 84-85 -1.0086051594203873 1101 86-87 -1.3944174229345414 1101 88-89 -0.6842394867443851 1101 90-91 -1.202013612864345 1101 92-93 -0.7202759727348003 1101 94-95 -0.5370048591378591 1101 96-97 -0.5702480602122968 1101 98-99 -0.5909548286574733 1101 100-101 -0.5371727187061559 1102 1 -0.10012831722058735 1102 2 -0.08310607503788958 1102 3 -0.2560066506633589 1102 4 -0.14357273090483602 1102 5 -0.17920294456522612 1102 6 -0.07145352703730623 1102 7 -0.16407985363603217 1102 8 -0.15925092712182476 1102 9 -0.285028996818518 1102 10-11 -0.19131785323203587 1102 12-13 -0.06953991536966697 1102 14-15 -0.1620416616496101 1102 16-17 -0.4574489033207456 1102 18-19 -0.487938653123706 1102 20-21 0.03517254964980765 1102 22-23 -0.27121568473156543 1102 24-25 -0.162265009559313 1102 26-27 -0.1262957398814777 1102 28-29 -0.09290019755978562 1102 30-31 -0.10509131460302967 1102 32-33 0.0026125494313973263 1102 34-35 -0.08098664519997811 1102 36-37 -0.056161191695679236 1102 38-39 0.15769061065415713 1102 40-41 -0.2672658212560748 1102 42-43 0.0743259155863285 1102 44-45 -0.4515852170279544 1102 46-47 -0.9341830599561334 1102 48-49 -0.9531367718142434 1102 50-51 -1.2473832055267806 1102 52-53 -1.3309354426271653 1102 54-55 -1.330890538009335 1102 56-57 -1.403835223116424 1102 58-59 -1.1050384897156178 1102 60-61 -1.0951911686724785 1102 62-63 -1.0208896853239153 1102 64-65 -1.350620680242855 1102 66-67 -1.291856080299894 1102 68-69 -1.525619096785931 1102 70-71 -1.3690406952121599 1102 72-73 -1.2760339975040935 1102 74-75 -1.18169976164409 1102 76-77 -1.0736217695431414 1102 78-79 -1.3522660925030756 1102 80-81 -1.232942886344599 1102 82-83 -1.1202573158434532 1102 84-85 -1.6115581561004504 1102 86-87 -1.2586960646725593 1102 88-89 -1.2395077027150307 1102 90-91 -0.8802942349157199 1102 92-93 -1.3227863111354061 1102 94-95 -1.2023610243192913 1102 96-97 -1.3973026353185922 1102 98-99 -1.6835344780255213 1102 100-101 -0.6254830454578624 1103 1 0.08819051869006245 1103 2 0.030752387161420813 1103 3 0.017659049508459645 1103 4 -0.05701774808696314 1103 5 0.09793945234198986 1103 6 -0.05145245315414826 1103 7 0.0052446738553797445 1103 8 -0.09163655856512065 1103 9 -0.1370747442411968 1103 10-11 0.043983370994766346 1103 12-13 -0.18665358590231307 1103 14-15 -0.12919426044686588 1103 16-17 -0.09157691019360215 1103 18-19 -0.2135682171271469 1103 20-21 -0.20468596624366597 1103 22-23 -0.09629354126077061 1103 24-25 -0.27966728619161785 1103 26-27 -0.03002882622168812 1103 28-29 0.020844164553615485 1103 30-31 -0.06446497224908398 1103 32-33 -0.03704729807719076 1103 34-35 0.10402999998903084 1103 36-37 -0.20813579435890972 1103 38-39 0.018461658764124422 1103 40-41 -0.188785097458819 1103 42-43 -0.025900942231544377 1103 44-45 -0.3957432926293265 1103 46-47 -0.29196737052314603 1103 48-49 -0.648610354288472 1103 50-51 -0.2326374473652706 1103 52-53 -0.3723671973522471 1103 54-55 -0.36902681378253277 1103 56-57 -0.28413752122639124 1103 58-59 -0.4077325940970624 1103 60-61 -0.6398579964216182 1103 62-63 -0.2968977931417882 1103 64-65 -0.7763817949335774 1103 66-67 -0.3083468985988631 1103 68-69 -1.008725572301394 1103 70-71 -0.831756814198414 1103 72-73 -0.46972224922230765 1103 74-75 -0.30080682779529155 1103 76-77 -0.1930174417940016 1103 78-79 -0.4062354331388143 1103 80-81 -0.6125672957088604 1103 82-83 -0.6890798029568543 1103 84-85 -0.49924340094581154 1103 86-87 -0.30916186149386604 1103 88-89 -0.36464864987997814 1103 90-91 -0.1637389836270593 1103 92-93 -0.08491447909073102 1103 94-95 0.18796865781129313 1103 96-97 0.33039415377075443 1103 98-99 0.28093341286795237 1103 100-101 0.9284191774802828 1104 1 -0.05438006528677164 1104 2 -0.17535885801232354 1104 3 -0.17350588775023112 1104 4 -0.18901581758503028 1104 5 -0.2549502677352322 1104 6 -0.17913705740124897 1104 7 -0.19175701533380618 1104 8 0.009518726183912918 1104 9 -0.3100658156697662 1104 10-11 -0.23245545622530983 1104 12-13 -0.4167980719061717 1104 14-15 -0.1965054332267897 1104 16-17 -0.4601821225488152 1104 18-19 -0.1615577803510888 1104 20-21 -0.1521325757996479 1104 22-23 0.1960719268859421 1104 24-25 -0.09080990202173922 1104 26-27 -0.384087404889641 1104 28-29 0.07282443723700993 1104 30-31 0.02285253499029949 1104 32-33 0.028989441787679482 1104 34-35 -0.030004145763861345 1104 36-37 -0.5299037118299523 1104 38-39 -0.13398766556019837 1104 40-41 -0.19226150913835482 1104 42-43 -0.1447097622122442 1104 44-45 -0.0221911690772032 1104 46-47 -0.15855912969302466 1104 48-49 -0.2523446689602835 1104 50-51 -4.1163896758433793E-4 1104 52-53 -0.18464850768043561 1104 54-55 -0.23882350779798145 1104 56-57 -0.1333924681375862 1104 58-59 -0.1682674034599998 1104 60-61 -0.09667055674980674 1104 62-63 0.009109385912260848 1104 64-65 -0.10906911394902608 1104 66-67 0.014476762115421593 1104 68-69 0.23846162605767773 1104 70-71 -0.0780390897775618 1104 72-73 -0.2670647926586014 1104 74-75 -0.3595513982586098 1104 76-77 0.07457576525233023 1104 78-79 -0.21697837474112802 1104 80-81 -0.4068406427165847 1104 82-83 -0.7299973946556975 1104 84-85 -0.4820951142662757 1104 86-87 -0.3694975878452169 1104 88-89 -0.35700959341279415 1104 90-91 -0.35227210380080365 1104 92-93 -0.03871063015250442 1104 94-95 -0.0797376645825274 1104 96-97 -0.446911891113416 1104 98-99 -0.17962703597374485 1104 100-101 -0.16761575253902095 1105 1 0.2449028911390414 1105 2 0.15756998920223708 1105 3 -0.053594139267048035 1105 4 -0.005232033801249258 1105 5 -0.010383251739639832 1105 6 -0.07080832050108654 1105 7 -0.19440456083849966 1105 8 -0.12318949223858766 1105 9 0.15932193943227446 1105 10-11 0.10802992711721515 1105 12-13 -0.07416155784108724 1105 14-15 0.018111224247014945 1105 16-17 0.15421390613293084 1105 18-19 -0.025374658453678478 1105 20-21 -0.25533169328448224 1105 22-23 -0.12003397493423762 1105 24-25 -0.07293896986508486 1105 26-27 0.11856014061504538 1105 28-29 0.18738593751280064 1105 30-31 0.1630621323427519 1105 32-33 0.14167559733097335 1105 34-35 0.3065650765196395 1105 36-37 0.60848569798803 1105 38-39 0.43115298529473733 1105 40-41 0.4893159867248542 1105 42-43 0.11761787154397041 1105 44-45 0.10731793186047156 1105 46-47 0.6194883054972635 1105 48-49 0.7888577579564284 1105 50-51 0.6182314939612539 1105 52-53 0.5006796776477529 1105 54-55 0.6480490790746103 1105 56-57 0.6206615859164657 1105 58-59 0.7456873676376361 1105 60-61 0.5662086489865459 1105 62-63 0.28158594410310656 1105 64-65 0.36759939129091634 1105 66-67 0.3083543131358297 1105 68-69 0.15693928739248264 1105 70-71 0.07458087712811334 1105 72-73 0.3202490517981005 1105 74-75 0.734381309959808 1105 76-77 0.5282437189202831 1105 78-79 0.03227700308567805 1105 80-81 0.1641466711278774 1105 82-83 0.20761981439008537 1105 84-85 0.25061310415622984 1105 86-87 0.41246600840409187 1105 88-89 0.028870163895529544 1105 90-91 -0.07648611372909997 1105 92-93 0.23531032958273812 1105 94-95 0.43449289250517253 1105 96-97 0.4025641155054487 1105 98-99 0.5375820098067265 1105 100-101 1.0271994708476306 1106 1 0.04745391154720835 1106 2 0.10552917287570551 1106 3 0.25928851379417495 1106 4 0.22423225191303686 1106 5 -0.19112304765801014 1106 6 0.15278861827442114 1106 7 -0.29721068328747435 1106 8 0.019412548577733446 1106 9 0.2077913271873726 1106 10-11 -0.021306807576664255 1106 12-13 0.07143793195482573 1106 14-15 0.12359591812456472 1106 16-17 0.06913737552068966 1106 18-19 0.12488044358713779 1106 20-21 -0.033648019815096575 1106 22-23 0.1788180658820906 1106 24-25 -0.07319407190590965 1106 26-27 -0.16479445122168812 1106 28-29 0.10817675383932368 1106 30-31 -0.07144041867765338 1106 32-33 -0.17516113736290606 1106 34-35 -0.13871553572526096 1106 36-37 0.2647994734982291 1106 38-39 0.15685451590698563 1106 40-41 0.3488823132554657 1106 42-43 -0.11044335294582908 1106 44-45 -0.0877075783436112 1106 46-47 0.18096789733399277 1106 48-49 0.3636664314258127 1106 50-51 0.10860139192044471 1106 52-53 -0.014945322352247103 1106 54-55 0.26545979336032843 1106 56-57 0.13941158591646996 1106 58-59 0.054041959474368184 1106 60-61 0.5975862000069512 1106 62-63 0.2988053318582118 1106 64-65 0.1943213300664226 1106 66-67 0.4363517621154216 1106 68-69 0.3436739812700367 1106 70-71 0.5747722036587248 1106 72-73 0.24065721506340765 1106 74-75 0.545733350776139 1106 76-77 0.3407437189202831 1106 78-79 0.40319537043261633 1106 80-81 0.8575777935768549 1106 82-83 0.45991573275743036 1106 84-85 0.46336820619704255 1106 86-87 0.37917519207756456 1106 88-89 0.36330893940573716 1106 90-91 0.6585266413729407 1106 92-93 0.7328868601949843 1106 94-95 0.7024775863827237 1106 96-97 1.0220794216278968 1106 98-99 1.3026968057250947 1106 100-101 2.264635695337425 1107 1 0.036353525446820356 1107 2 -0.056311238964703136 1107 3 0.11285189860755906 1107 4 0.21381558524637256 1107 5 0.1723341595491945 1107 6 0.001686623422429534 1107 7 0.07400875043195754 1107 8 0.024319240984425505 1107 9 -0.008907514511470538 1107 10-11 0.01143128766143775 1107 12-13 -0.01525367036177272 1107 14-15 0.2825807958594453 1107 16-17 0.3737947114280189 1107 18-19 0.3532427344494309 1107 20-21 0.3256666519995761 1107 22-23 0.252699983514006 1107 24-25 0.20745264624081727 1107 26-27 0.040301269499032344 1107 28-29 0.24721311162569037 1107 30-31 0.1316041437419102 1107 32-33 0.017728180526411563 1107 34-35 0.6225055067457888 1107 36-37 0.2850898531636119 1107 38-39 0.20385017227764024 1107 40-41 0.19802163114478333 1107 42-43 0.12909976159728842 1107 44-45 0.3474999506139156 1107 46-47 0.016151295017394318 1107 48-49 0.497011830396211 1107 50-51 0.44006455150860546 1107 52-53 0.5412202181882932 1107 54-55 0.5700568010823304 1107 56-57 0.8135187287736088 1107 58-59 0.5656450804524908 1107 60-61 0.9422612321819841 1107 62-63 0.9032535300564106 1107 64-65 0.8687301138502121 1107 66-67 0.7287624764011369 1107 68-69 0.8295169671130154 1107 70-71 1.1491166373781638 1107 72-73 1.1344796088858047 1107 74-75 1.4001011113939015 1107 76-77 1.0398267305032967 1107 78-79 1.322596914834154 1107 80-81 1.603455367579425 1107 82-83 1.086926672268369 1107 84-85 1.3415857094145487 1107 86-87 1.5111330427854135 1107 88-89 1.707018720615519 1107 90-91 1.4154241638954623 1107 92-93 1.3792430378011602 1107 94-95 1.4633768785320136 1107 96-97 1.2635128193112948 1107 98-99 1.6520975457508342 1107 100-101 2.310826975903705 1108 1 -0.15937036746016986 1108 2 -0.06398792973998724 1108 3 -0.049627482181684 1108 4 -0.19932995465973136 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-0.33711693919711294 2316 94-95 0.24265615781129313 2316 96-97 0.4091424915367128 2316 98-99 -0.4063172520256657 2316 100-101 -2.2242985751792936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 8.0 7 20.0 8 57.0 9 85.0 10 160.0 11 259.0 12 447.0 13 612.0 14 784.0 15 1122.0 16 1435.0 17 2021.0 18 1881.0 19 1147.0 20 1045.0 21 1003.0 22 1111.0 23 1043.0 24 1268.0 25 1546.0 26 1838.0 27 2369.0 28 2894.0 29 3542.0 30 4516.0 31 5718.0 32 7412.0 33 9599.0 34 13226.0 35 19324.0 36 29802.0 37 45262.0 38 36870.0 39 3269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.21904141304938 20.46315082761923 16.470437233732703 21.847370525598684 2 15.30222599360619 20.874610253779057 40.513872992066936 23.309290760547814 3 18.859276946757706 24.954118482851168 31.146149899356672 25.040454671034457 4 12.6248174606307 18.121225875202274 38.370762126534316 30.883194537632715 5 13.147469325531716 37.8646524546975 34.81570651181837 14.172171707952423 6 30.28574811540435 37.97410901053795 17.578541263764453 14.161601610293248 7 27.13127047401034 33.90693452263488 21.159766349607292 17.802028653747485 8 26.068595334885742 35.35540908552709 19.790227730196946 18.78576784939022 9 25.62606070174054 17.297825314757077 21.08971069976714 35.98640328373525 10-11 24.288836484193077 27.553577771638317 27.593045743379246 20.564540000789357 12-13 25.48621526681402 24.666975844647933 28.26931046731923 21.57749842121882 14-15 22.26314283458973 26.11817697438529 26.899149465208982 24.719530725816 16-17 21.165686545368434 29.377491415716143 27.714409756482617 21.742412282432806 18-19 21.111171409401273 27.25312783676047 30.723842601728695 20.911858152109563 20-21 21.661996290010656 26.342157714015073 31.272694083751034 20.72315191222323 22-23 21.28454714989097 26.42135435681372 31.25647527800527 21.037623215290044 24-25 21.21789195069427 26.55696133836883 31.25861826541914 20.966528445517763 26-27 21.116403669543722 26.873561573887926 30.93926604192002 21.070768714648327 28-29 20.97878292273195 27.01951990843182 30.953350058340177 21.048347110496053 30-31 21.18201933442035 26.75836414720603 30.86205663174565 21.19755988662797 32-33 20.9113648024628 26.809853179145122 30.735189643604215 21.54359237478786 34-35 21.4838970675297 26.933437265658917 30.644166633776692 20.938499033034695 36-37 21.560119587954375 26.43663417136993 30.892321506097804 21.11092473457789 38-39 21.179851501023705 26.95947112656948 30.65196477466144 21.20871259774538 40-41 21.541372301377432 26.85943481864467 30.587431424399096 21.0117614555788 42-43 21.215388107126646 26.81940634312894 30.547524127178356 21.417681422566066 44-45 21.422527880490687 26.98308546563688 30.435308134615717 21.15907851925672 46-47 21.84897580613332 27.25904803252161 29.66856770730552 21.223408454039546 48-49 21.582813671705413 27.426046887950427 29.53141650550578 21.45972293483838 50-51 21.53841220349686 27.405079527963057 29.65968741366381 21.396820854876268 52-53 21.983660259699253 27.424566839010144 29.32445632868927 21.267316572601334 54-55 21.58824051781979 27.586878872794728 29.3703378458381 21.454542763547384 56-57 21.720458223151912 27.616973201247184 29.449273789319967 21.213294786280933 58-59 21.687403796818884 27.478835300153925 29.325936377629553 21.50782452539764 60-61 21.328245253976398 27.787425504203338 29.652040494139005 21.232288747681256 62-63 21.32232505821526 27.736610490586887 29.66856770730552 21.27249674389233 64-65 21.41137466945574 27.709722934838375 29.415972688163556 21.46292970754233 66-67 21.378320243122705 27.73735051505703 29.290908552709478 21.593420689110786 68-69 21.621788293799582 27.42752693689071 29.347890436910447 21.60279433239926 70-71 21.67457670600308 27.664088092512927 29.114536053992186 21.54679914749181 72-73 21.58256699688203 27.404832853139677 29.105655760350473 21.906944389627817 74-75 21.537178829379958 27.747464182815644 28.99267869124206 21.72267829656234 76-77 21.51744484350949 27.541490705292652 29.11576942810909 21.825295023088763 78-79 21.637822157319338 27.76769151833287 28.97935825077949 21.6151280735683 80-81 21.7463590796069 27.837993842996404 28.69765560247859 21.717991474918104 82-83 21.7771934325295 27.809132888660855 28.822966412756045 21.590707266053595 84-85 21.512124127383508 27.956042329608525 28.91339204223094 21.61844150077703 86-87 21.3822670402968 27.89250897896357 28.764997829261553 21.960226151478075 88-89 21.498204207285788 27.902869321545566 28.57037139361408 22.028555077554564 90-91 21.43091500704307 27.783492820015642 28.716483744199646 22.069108428741647 92-93 21.494981289299258 27.472784995917443 29.0836073540331 21.948626360750204 94-95 21.309744642222835 27.53458381023799 29.09529541776848 22.06037612977069 96-97 21.38251371512018 27.270148399573745 29.156470773966923 22.19086711133915 98-99 21.069730039073292 27.384358842799067 28.806685874412914 22.739225243714724 100-101 21.063889101328996 27.241373355405173 29.04823815879391 22.646499384471916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 17.5 2 26.0 3 35.0 4 27.5 5 25.0 6 53.5 7 142.5 8 254.5 9 307.5 10 265.0 11 210.5 12 214.5 13 237.0 14 245.0 15 260.5 16 292.5 17 321.0 18 346.0 19 375.0 20 425.5 21 504.0 22 592.5 23 706.0 24 862.0 25 1065.0 26 1317.5 27 1659.0 28 2058.0 29 2439.5 30 2811.5 31 3300.5 32 3961.5 33 4411.5 34 4839.5 35 5358.0 36 5967.0 37 6403.0 38 6600.0 39 6876.0 40 7099.0 41 7314.5 42 7337.0 43 7344.0 44 7460.0 45 7613.0 46 7685.5 47 7706.5 48 7771.5 49 7511.0 50 6966.0 51 6531.5 52 6205.5 53 5901.5 54 5696.5 55 5235.5 56 4488.0 57 3958.0 58 3661.5 59 3202.5 60 2522.0 61 2024.0 62 1647.5 63 1174.0 64 766.0 65 534.0 66 388.5 67 296.0 68 241.0 69 180.0 70 120.0 71 78.5 72 61.0 73 42.0 74 31.0 75 29.5 76 19.5 77 11.5 78 8.5 79 4.0 80 2.5 81 1.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06265540513872993 2 0.0 3 0.0 4 0.0 5 0.0014800489402849587 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0039467971740932235 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.866992935233059E-4 24-25 7.400244701424794E-4 26-27 2.4667482338082647E-4 28-29 0.002713423057189091 30-31 2.4667482338082647E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.933496467616529E-4 40-41 0.0 42-43 0.009866992935233057 44-45 2.4667482338082647E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 4.933496467616529E-4 86-87 0.0 88-89 0.0 90-91 0.007153569878043968 92-93 0.002220073410427438 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.012087066345660495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 202696.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.07065051267848 #Duplication Level Percentage of deduplicated Percentage of total 1 85.6410787969148 62.57849338298121 2 9.554661156392111 13.963306122515847 3 2.1451699357803946 4.702468880031425 4 0.9445010632339511 2.760612284016851 5 0.4572722356488518 1.6706589860124208 6 0.28809535732243313 1.2630789101539643 7 0.20162738310333922 1.0313130831171025 8 0.13849948063813952 0.8096197716717579 9 0.08246535861181276 0.5423217658673823 >10 0.5063940488334164 6.336232470463744 >50 0.024533648488270405 1.1638141236931612 >100 0.01275749721390061 1.353839227068816 >500 0.0019626918790616324 1.0713113375216161 >1k 9.813459395308162E-4 0.75292965488467 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1050 0.5180171290997355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 945 0.46621541618976203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 549 0.2708489560721475 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 212 0.1045901251134704 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.933496467616529E-4 0.0 24-25 0.0 0.0 0.0 7.400244701424794E-4 0.0 26-27 0.0 0.0 0.0 0.0012333741169041324 0.0 28-29 0.0 0.0 0.0 0.0017267237636657852 0.0 30-31 0.0 0.0 0.0 0.0037001223507123968 0.0 32-33 0.0 0.0 0.0 0.007646919524805621 0.0 34-35 4.933496467616529E-4 0.0 0.0 0.011593716698898844 0.0 36-37 4.933496467616529E-4 0.0 0.0 0.014060464932707108 0.0 38-39 4.933496467616529E-4 0.0 0.0 0.02170738445751273 0.0 40-41 4.933496467616529E-4 0.0 0.0 0.027627580218652564 0.0 42-43 4.933496467616529E-4 0.0 0.0 0.0429214192682638 0.0 44-45 4.933496467616529E-4 0.0 0.0 0.06487547854915736 0.0 46-47 4.933496467616529E-4 0.0 0.0 0.09398310770809488 0.0 48-49 4.933496467616529E-4 0.0 0.0 0.14381142203102182 0.0 50-51 4.933496467616529E-4 0.0 0.0 0.19585980976437622 0.0 52-53 4.933496467616529E-4 0.0 0.0 0.2518549946718238 0.0 54-55 4.933496467616529E-4 0.0 0.0 0.32881753956664167 0.0 56-57 4.933496467616529E-4 0.0 0.0 0.4082468326952678 0.0 58-59 4.933496467616529E-4 0.0 0.0 0.4763290839483759 0.0 60-61 4.933496467616529E-4 0.0 0.0 0.5476181079054347 0.0 62-63 4.933496467616529E-4 0.0 0.0 0.663801949717804 0.0 64-65 4.933496467616529E-4 0.0 0.0 0.7595117811895646 0.0 66-67 4.933496467616529E-4 0.0 0.0 0.8766823222954572 0.0 68-69 4.933496467616529E-4 0.0 0.0 1.0380076567865177 0.0 70-71 4.933496467616529E-4 0.0 0.0 1.172692110352449 2.4667482338082647E-4 72-73 4.933496467616529E-4 0.0 0.0 1.3601649761218773 7.400244701424794E-4 74-75 4.933496467616529E-4 0.0 0.0 1.600426254094802 9.866992935233059E-4 76-77 4.933496467616529E-4 0.0 0.0 1.7834589730433752 9.866992935233059E-4 78-79 4.933496467616529E-4 0.0 0.0 1.9909124995066505 9.866992935233059E-4 80-81 4.933496467616529E-4 0.0 0.0 2.2790286932154555 9.866992935233059E-4 82-83 4.933496467616529E-4 0.0 0.0 2.5903323203220587 9.866992935233059E-4 84-85 4.933496467616529E-4 0.0 0.0 2.915203062714607 9.866992935233059E-4 86-87 7.400244701424794E-4 0.0 0.0 3.2973023641315073 9.866992935233059E-4 88-89 9.866992935233059E-4 0.0 0.0 3.7242964834037178 9.866992935233059E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCCGT 25 0.005016563 56.996445 6 GGTATCA 505 0.0 51.767765 1 AGCGTCG 30 8.2842904E-5 39.580868 26-27 GTATCAA 835 0.0 37.57038 1 GTGTTAT 55 0.0025660829 34.56889 1 GTAGCGT 35 2.0502604E-4 33.93064 24-25 CGTCGTG 35 2.0517473E-4 33.926456 28-29 CCTAGAC 60 0.003944546 31.664696 2 TAGGACC 65 0.0058346437 29.228949 4 TCAACGC 1080 0.0 29.025967 4 ATCAACG 1090 0.0 28.759674 3 TATCAAC 1080 0.0 28.586178 2 TGGTATC 255 0.0 27.939436 2 CAACGCA 1125 0.0 27.86493 5 AACGCAG 1145 0.0 27.378206 6 AGTAGCG 35 0.008377734 27.147861 22-23 GCGTCGT 35 0.008387825 27.141165 26-27 GTAAGGT 125 7.3552364E-6 26.598343 4 TCGTGGT 45 8.940767E-4 26.387245 30-31 GTGTAAG 100 0.0016424657 23.76611 1 >>END_MODULE