FastQCFastQC Report
Thu 26 May 2016
SRR937223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985306
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62350.31405737956768376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46530.23437193057392666No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38330.19306847407905883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32230.16234273205238892No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31840.16037829936543788No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22340.11252673391406665No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22210.1118719230184163No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21680.10920230936691876No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21490.10824527805789133No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20800.10476974330405489No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19930.1003875473100872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116750.025.4111421
GTACATG79150.024.3877451
TACATGG78800.024.3600042
GGTATCA86200.024.32361
TAAGGTA6950.023.9273434
GTGTAGG5700.023.354991
GTACGCT1503.0288942E-522.172113
ACATGGG81800.022.0133513
TAAGGTG11550.021.8029925
GAGTACT67200.021.62931412-13
ATCAACG138200.021.280553
TCAACGC138700.021.1701164
TATCAAC140150.021.121132
CAACGCA139150.020.9997485
AGTACTT71200.020.8478212-13
GTACTTT69200.020.83153514-15
AACGCAG141700.020.5851946
GTAAGGT11150.020.453974
AGAGTAC108750.020.3996210-11
GGACCGA4300.019.8865976
Produced by FastQC (version 0.11.5)