##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937223_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985306 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.756706019122493 33.0 31.0 34.0 30.0 34.0 2 31.87598234226865 33.0 31.0 34.0 30.0 34.0 3 31.919269875777335 33.0 31.0 34.0 30.0 34.0 4 35.54970367288468 37.0 35.0 37.0 33.0 37.0 5 35.55149432883394 37.0 35.0 37.0 33.0 37.0 6 35.59252377215402 37.0 35.0 37.0 33.0 37.0 7 35.52845505932083 37.0 35.0 37.0 33.0 37.0 8 35.49744170420076 37.0 35.0 37.0 33.0 37.0 9 37.103667142495915 39.0 37.0 39.0 33.0 39.0 10-11 36.98843301737868 39.0 37.0 39.0 33.0 39.0 12-13 36.84983649875636 39.0 37.0 39.0 33.0 39.0 14-15 37.96871263170514 40.0 38.0 41.0 33.0 41.0 16-17 37.886444205578385 40.0 38.0 41.0 33.0 41.0 18-19 37.79209048882137 40.0 38.0 41.0 33.0 41.0 20-21 37.634318588670965 40.0 37.0 41.0 32.0 41.0 22-23 37.709094718899756 40.0 37.0 41.0 32.0 41.0 24-25 37.668052179361766 40.0 37.0 41.0 32.0 41.0 26-27 37.56970990869921 40.0 37.0 41.0 32.0 41.0 28-29 37.43340396895995 40.0 37.0 41.0 32.0 41.0 30-31 37.16362893176165 39.0 36.5 41.0 31.0 41.0 32-33 36.9040236114735 39.0 36.0 41.0 31.0 41.0 34-35 36.91853119871698 39.0 36.0 41.0 31.0 41.0 36-37 36.777476368882176 39.0 36.0 41.0 30.0 41.0 38-39 36.62373608904622 39.0 36.0 41.0 30.0 41.0 40-41 36.3867834479924 39.0 35.0 40.5 30.0 41.0 42-43 36.09624108323855 39.0 35.0 40.0 29.5 41.0 44-45 35.84438368694801 39.0 35.0 40.0 28.5 41.0 46-47 35.45739573647589 38.0 35.0 40.0 27.0 41.0 48-49 35.09408549614014 38.0 34.5 40.0 26.0 41.0 50-51 34.09712381869596 37.5 33.5 39.5 24.5 40.5 52-53 34.18610002689762 37.5 33.5 39.5 24.0 40.0 54-55 34.45246098082613 38.0 34.0 40.0 24.0 41.0 56-57 34.130050732733395 38.0 33.5 40.0 23.0 41.0 58-59 33.811330092187305 37.5 33.0 40.0 22.5 41.0 60-61 33.4270243478839 37.0 33.0 40.0 22.0 41.0 62-63 33.00178838929616 36.0 32.0 39.0 20.5 41.0 64-65 32.51825864627418 36.0 31.5 39.0 18.5 40.0 66-67 32.03285135893409 35.0 31.0 38.5 17.5 40.0 68-69 31.49996121504695 35.0 31.0 38.0 12.5 40.0 70-71 30.980600975366016 35.0 30.0 37.0 8.0 39.5 72-73 30.465140638269368 34.0 29.5 37.0 7.0 39.0 74-75 29.899483757163882 34.0 29.0 36.0 2.0 39.0 76-77 29.352829488250173 34.0 29.0 35.5 2.0 38.0 78-79 28.783118823999928 34.0 28.0 35.0 2.0 37.0 80-81 28.19738947043932 33.5 26.5 35.0 2.0 37.0 82-83 27.617603785008455 33.0 26.0 35.0 2.0 36.0 84-85 27.017330074054076 32.5 25.5 35.0 2.0 36.0 86-87 26.406912586775036 32.0 24.5 35.0 2.0 35.0 88-89 25.779387661146444 32.0 22.5 34.0 2.0 35.0 90-91 25.15842368884192 31.5 19.5 34.0 2.0 35.0 92-93 24.438481271904685 31.0 16.5 34.0 2.0 35.0 94-95 23.57924319978885 31.0 4.5 34.0 2.0 35.0 96-97 22.63595410480802 30.0 2.0 34.0 2.0 35.0 98-99 21.36908315393194 29.5 2.0 34.0 2.0 35.0 100-101 18.30323310361224 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10787214765478836 1101 2 0.12135287584952437 1101 3 0.09280681996355611 1101 4 -0.03279702137163554 1101 5 0.07153536221681378 1101 6 -0.02089889342445872 1101 7 0.12635250739829473 1101 8 0.11172575485980474 1101 9 0.09602867276687732 1101 10-11 0.18289821676106754 1101 12-13 0.25074841324681074 1101 14-15 0.2471412059438478 1101 16-17 0.33478971434648486 1101 18-19 0.38639272401842106 1101 20-21 0.4345865168660694 1101 22-23 0.28492519505991964 1101 24-25 0.20236785724039663 1101 26-27 0.19009847408387515 1101 28-29 0.09919290661592584 1101 30-31 0.14856032390762408 1101 32-33 0.15132094315615774 1101 34-35 0.1761911659527584 1101 36-37 0.1181386372072879 1101 38-39 0.2583373700598415 1101 40-41 0.28752960594513866 1101 42-43 0.39020413560595557 1101 44-45 0.22451473391416243 1101 46-47 0.1327472131120615 1101 48-49 0.15173903079128337 1101 50-51 0.01869858482636033 1101 52-53 0.050643422721563525 1101 54-55 0.2500181955108687 1101 56-57 0.2117891704422874 1101 58-59 0.29078529836877465 1101 60-61 0.36556292257940015 1101 62-63 0.4354643536050631 1101 64-65 0.2701127232296443 1101 66-67 0.4032194126438604 1101 68-69 0.32922132918811897 1101 70-71 0.381921263438862 1101 72-73 0.37800537380985233 1101 74-75 0.4553555127288007 1101 76-77 0.4607136793742548 1101 78-79 0.7278821578753139 1101 80-81 0.9551098678745547 1101 82-83 0.7469882368832153 1101 84-85 0.955732451197612 1101 86-87 1.063892602094949 1101 88-89 1.2051930223989338 1101 90-91 1.0444050718567404 1101 92-93 1.3558487299780246 1101 94-95 1.588488006653776 1101 96-97 1.4837312116562664 1101 98-99 2.0029934546311985 1101 100-101 2.0578243128486164 1102 1 0.13399798940562846 1102 2 0.15665380177545174 1102 3 0.06076767854941423 1102 4 0.083795992096384 1102 5 0.05406903225048154 1102 6 -0.006252428777990815 1102 7 -0.0771512804804928 1102 8 0.005381057890105012 1102 9 0.17643692192513072 1102 10-11 0.07733213931999217 1102 12-13 0.19051061863401486 1102 14-15 0.3732828305229674 1102 16-17 0.3678915661983311 1102 18-19 0.4233087593719631 1102 20-21 0.2330292778088321 1102 22-23 0.24895612940336065 1102 24-25 0.21233734377238278 1102 26-27 0.2994945178549102 1102 28-29 0.29637304129605724 1102 30-31 0.24398856464836882 1102 32-33 0.3114324751426949 1102 34-35 0.36634794204703525 1102 36-37 0.34504835942950507 1102 38-39 0.3192590872315648 1102 40-41 0.3623559948340258 1102 42-43 0.5935185295453422 1102 44-45 0.4048914174158469 1102 46-47 0.39319544543530327 1102 48-49 0.5690212277609845 1102 50-51 0.3563827178229886 1102 52-53 0.27671665504480103 1102 54-55 0.5962566214367868 1102 56-57 0.5844796165702348 1102 58-59 0.5543795744630486 1102 60-61 0.5434406582696383 1102 62-63 0.45224676101246786 1102 64-65 0.4782145750815019 1102 66-67 0.7113475692095115 1102 68-69 0.7713509588177487 1102 70-71 0.6340151186577181 1102 72-73 0.8299677054596835 1102 74-75 0.7287088376446249 1102 76-77 0.8120078712934458 1102 78-79 0.8065122083803651 1102 80-81 0.8080349520496384 1102 82-83 0.9828994321694076 1102 84-85 0.8805377963154584 1102 86-87 1.0618250515898957 1102 88-89 1.1145364567423677 1102 90-91 1.0431319237085894 1102 92-93 1.2127195212238142 1102 94-95 1.1732996649029346 1102 96-97 1.0971518766394297 1102 98-99 1.1999421078298482 1102 100-101 1.2525160216028226 1103 1 0.29064689863161774 1103 2 0.34064804397455717 1103 3 0.27063825466720814 1103 4 0.3358102374473617 1103 5 0.25009598078486306 1103 6 0.2129379895208956 1103 7 0.19007217059790094 1103 8 0.23179757259135414 1103 9 0.18513975636162172 1103 10-11 0.3620273923705568 1103 12-13 0.30800310478827697 1103 14-15 0.4587039295143711 1103 16-17 0.4230240575306823 1103 18-19 0.3244478668283932 1103 20-21 0.33690889868325513 1103 22-23 0.14458860103648163 1103 24-25 0.29924784458285814 1103 26-27 0.21927717916339162 1103 28-29 0.07009090342402402 1103 30-31 0.28760158470780794 1103 32-33 0.1997920647234963 1103 34-35 0.3330207295430938 1103 36-37 0.3824547177752251 1103 38-39 0.38489548463801526 1103 40-41 0.4141723333679508 1103 42-43 0.32500477948906337 1103 44-45 0.3777128242779284 1103 46-47 0.2959973782102949 1103 48-49 0.40008598472887513 1103 50-51 0.2326466339155715 1103 52-53 0.17217429609122092 1103 54-55 0.45448238295239207 1103 56-57 0.41435679192707653 1103 58-59 0.5327033958867986 1103 60-61 0.35283556855375764 1103 62-63 0.4206794628450652 1103 64-65 0.48819785888536416 1103 66-67 0.623193203299298 1103 68-69 0.5134726774903591 1103 70-71 0.7503383153347407 1103 72-73 0.8058344008309284 1103 74-75 0.5713053778985753 1103 76-77 0.5613162879263456 1103 78-79 0.7580528281741401 1103 80-81 0.7661009388117641 1103 82-83 0.9940625586046359 1103 84-85 0.9999646756211469 1103 86-87 1.0595205945004302 1103 88-89 1.1087828771124855 1103 90-91 1.0110074190032918 1103 92-93 1.3489431524093725 1103 94-95 1.324348718777486 1103 96-97 1.3602198473945286 1103 98-99 1.377479930334239 1103 100-101 1.077059632864021 1104 1 0.2057714613956847 1104 2 0.17219040007814002 1104 3 0.17128788235551085 1104 4 0.2763489527495864 1104 5 0.14492186753400915 1104 6 0.12614652540313642 1104 7 0.17696542583299646 1104 8 0.12024767848073736 1104 9 0.20266141937487703 1104 10-11 0.20511880191481424 1104 12-13 0.21872080572415342 1104 14-15 0.49072749909696256 1104 16-17 0.38247784625491477 1104 18-19 0.40269006803853813 1104 20-21 0.2586684488687965 1104 22-23 0.30977884113881515 1104 24-25 0.3937198793473655 1104 26-27 0.4099852544285767 1104 28-29 0.43001497691818713 1104 30-31 0.4339527619609598 1104 32-33 0.4963681562107993 1104 34-35 0.49940086787533033 1104 36-37 0.6360998976190402 1104 38-39 0.6318541860271552 1104 40-41 0.6997447560315706 1104 42-43 0.779825776341454 1104 44-45 0.8429854500333889 1104 46-47 0.7952992718893697 1104 48-49 0.7938123452655859 1104 50-51 0.7819575297684693 1104 52-53 0.7648721670245706 1104 54-55 0.8496858706117081 1104 56-57 0.9187548967768322 1104 58-59 1.0294034161329293 1104 60-61 1.108131613972347 1104 62-63 1.0471533498856402 1104 64-65 1.087817946132894 1104 66-67 1.0575088594456794 1104 68-69 0.9109225052581138 1104 70-71 0.8565201816640204 1104 72-73 1.0302943229428614 1104 74-75 0.9648937284551309 1104 76-77 1.1334545248873766 1104 78-79 1.2557984316455482 1104 80-81 1.0768216521482223 1104 82-83 1.1968107786611988 1104 84-85 1.324852428671555 1104 86-87 1.3599567620823727 1104 88-89 1.313998813692347 1104 90-91 1.4407848586449425 1104 92-93 1.4586229062051714 1104 94-95 1.4268333888110405 1104 96-97 1.3762517732362305 1104 98-99 1.6298770656113426 1104 100-101 1.5799823619868612 1105 1 0.027878990525369574 1105 2 -0.024997949571741174 1105 3 0.07205367651988226 1105 4 0.059339023449169304 1105 5 0.06274441191316527 1105 6 0.08219322872078294 1105 7 0.08189523257150455 1105 8 0.15146786417466274 1105 9 0.09837556353755872 1105 10-11 0.16359757951874343 1105 12-13 0.22539343933525657 1105 14-15 0.28848978604820275 1105 16-17 0.23664637759171114 1105 18-19 0.23799652181159558 1105 20-21 0.30006423476873323 1105 22-23 0.32492551581948703 1105 24-25 0.28307149679218213 1105 26-27 0.32580168595625025 1105 28-29 0.3515483509748307 1105 30-31 0.37950025633428197 1105 32-33 0.39350456731149563 1105 34-35 0.48689598162292924 1105 36-37 0.38384623279363694 1105 38-39 0.5418791971062902 1105 40-41 0.45418985742063 1105 42-43 0.4411375459215776 1105 44-45 0.5242378114952047 1105 46-47 0.5310552064402643 1105 48-49 0.5564445735164583 1105 50-51 0.5061461940984486 1105 52-53 0.535686404502421 1105 54-55 0.7004774162789786 1105 56-57 0.7226570388881015 1105 58-59 0.6439795289735102 1105 60-61 0.8056477100227326 1105 62-63 0.70522260781771 1105 64-65 0.7081270077219912 1105 66-67 0.8482749040475035 1105 68-69 0.7091829449609364 1105 70-71 0.8139977539016137 1105 72-73 0.9378617804474381 1105 74-75 1.1115034042692997 1105 76-77 1.1170005667234975 1105 78-79 1.1518348779163716 1105 80-81 1.1126600132855522 1105 82-83 1.2308294395177164 1105 84-85 1.332821130523591 1105 86-87 1.1927401130007702 1105 88-89 1.1693561607104535 1105 90-91 1.3496682016146728 1105 92-93 1.5417796811370366 1105 94-95 1.650255605661556 1105 96-97 1.5791101356148651 1105 98-99 1.6677521365232515 1105 100-101 1.6577238081869083 1106 1 0.03445378766055285 1106 2 -0.4511809907018538 1106 3 -0.01976343511642753 1106 4 0.14790805140542318 1106 5 0.11437294688319355 1106 6 0.05904057912201921 1106 7 0.0838427797718353 1106 8 0.2029384656250457 1106 9 0.227407473746851 1106 10-11 -0.20530883756952534 1106 12-13 -0.07990081574872221 1106 14-15 -0.009234892917660886 1106 16-17 0.07695447151610324 1106 18-19 0.14523405562239589 1106 20-21 0.1629461508190957 1106 22-23 0.29485007616671055 1106 24-25 0.14397958083169016 1106 26-27 0.08820789186741251 1106 28-29 0.38451860196107646 1106 30-31 0.34530787398689 1106 32-33 0.3206239767344883 1106 34-35 0.5564152616437852 1106 36-37 0.6138855027498735 1106 38-39 0.6055866134666772 1106 40-41 0.5376052652620373 1106 42-43 0.6026879228951856 1106 44-45 0.6124380658063657 1106 46-47 0.5062367288924392 1106 48-49 0.7555328051103487 1106 50-51 0.597326629213164 1106 52-53 0.727124183638125 1106 54-55 0.8323238231229126 1106 56-57 0.8232182308256313 1106 58-59 0.5908618583918326 1106 60-61 0.7159042902116255 1106 62-63 0.71929037320443 1106 64-65 0.7372531613331219 1106 66-67 0.9084047779702722 1106 68-69 0.9623772234091916 1106 70-71 0.8981330734979487 1106 72-73 0.9277847008022135 1106 74-75 0.7525092812833449 1106 76-77 0.7691713101567892 1106 78-79 1.0046628539410314 1106 80-81 0.9519006518491899 1106 82-83 0.9991103005811546 1106 84-85 0.8045858522640543 1106 86-87 0.8426080149786479 1106 88-89 1.2134155688753694 1106 90-91 1.216628194785116 1106 92-93 1.2145342394174286 1106 94-95 1.1180023458957393 1106 96-97 1.0093130678315063 1106 98-99 1.1060999901550481 1106 100-101 0.8293836153417686 1107 1 0.2134166448391568 1107 2 0.27776609315502654 1107 3 0.1645735223572693 1107 4 0.2723997751033025 1107 5 0.15040068826863262 1107 6 0.23425090497293155 1107 7 0.2848045055609063 1107 8 0.2917632682574478 1107 9 0.32656402552892416 1107 10-11 0.23748159262660096 1107 12-13 0.3436110852895453 1107 14-15 0.3443699143260943 1107 16-17 0.557650025852297 1107 18-19 0.4756695065427934 1107 20-21 0.39815173573675366 1107 22-23 0.5241729493599294 1107 24-25 0.4464925658948502 1107 26-27 0.43466041372217745 1107 28-29 0.5015761982676992 1107 30-31 0.4982841836194254 1107 32-33 0.43998132781735677 1107 34-35 0.6265348261034873 1107 36-37 0.5511076822743988 1107 38-39 0.7068680953188604 1107 40-41 0.6651989266208886 1107 42-43 0.6430386938665507 1107 44-45 0.821740996695091 1107 46-47 0.999970519905311 1107 48-49 0.9148246991422226 1107 50-51 0.8078128943586762 1107 52-53 0.7945577033319893 1107 54-55 0.8395589966473267 1107 56-57 0.8325968781270063 1107 58-59 0.894305156651491 1107 60-61 0.8882382190288141 1107 62-63 1.0070263698013306 1107 64-65 0.7280471045563033 1107 66-67 1.0030602358970562 1107 68-69 1.1375802881700707 1107 70-71 0.941505575012723 1107 72-73 1.0657421718159412 1107 74-75 1.0955046306110354 1107 76-77 1.1192473393548603 1107 78-79 0.952602402350383 1107 80-81 1.075746808788825 1107 82-83 1.1557870851113243 1107 84-85 1.1371057945685479 1107 86-87 1.1238588527967295 1107 88-89 0.976394152904728 1107 90-91 0.8893485253009956 1107 92-93 1.0858614683709895 1107 94-95 1.2003719277820686 1107 96-97 1.231082731756981 1107 98-99 1.276805424042042 1107 100-101 1.1905115821114478 1108 1 0.049959062155494394 1108 2 0.12721434076271265 1108 3 0.05916541994284685 1108 4 0.1601532564924355 1108 5 -0.07595264302980809 1108 6 0.0535911138241687 1108 7 0.08632454840481785 1108 8 -0.06241994256175332 1108 9 0.07952035410338709 1108 10-11 0.1189189357559215 1108 12-13 0.22190491457572392 1108 14-15 0.12586669867795308 1108 16-17 0.16575192655869841 1108 18-19 0.08381946110033311 1108 20-21 0.05256138828922019 1108 22-23 0.008698753100723877 1108 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-0.4974759097246917 2316 48-49 -0.46212778421190137 2316 50-51 -0.3210002685088611 2316 52-53 -0.1422743917871614 2316 54-55 -0.14462300083487634 2316 56-57 -0.24612372894787882 2316 58-59 -0.18520375887027996 2316 60-61 -0.27982201655553496 2316 62-63 -0.20205963335073562 2316 64-65 -0.015156637039716259 2316 66-67 0.08662735571089186 2316 68-69 -0.05229414607735805 2316 70-71 0.17371169102474582 2316 72-73 0.12157642817635761 2316 74-75 0.18169642770607908 2316 76-77 0.40490030083360296 2316 78-79 0.35440039973446247 2316 80-81 0.4881181264509955 2316 82-83 0.44132878690558286 2316 84-85 0.7751178847875941 2316 86-87 1.2174840233000062 2316 88-89 0.9299170988275556 2316 90-91 0.9071291953763172 2316 92-93 1.0520624699417631 2316 94-95 1.0948882562358442 2316 96-97 1.361699119169625 2316 98-99 1.8207436547677602 2316 100-101 2.2669440651501844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7264.0 3 1302.0 4 1280.0 5 1585.0 6 1785.0 7 2284.0 8 2791.0 9 3550.0 10 4500.0 11 5819.0 12 7801.0 13 9472.0 14 10859.0 15 13483.0 16 16961.0 17 21037.0 18 18853.0 19 15352.0 20 15983.0 21 17253.0 22 19172.0 23 21596.0 24 24720.0 25 27812.0 26 32736.0 27 37930.0 28 45232.0 29 54082.0 30 64941.0 31 79477.0 32 99481.0 33 126481.0 34 164623.0 35 220710.0 36 288460.0 37 316213.0 38 169710.0 39 12716.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.609033951580884 18.38096739221588 14.905232017119769 23.104766639083472 2 15.3706232401052 19.686733675650125 39.70534765623422 25.237295428010455 3 19.234067179328694 23.657090107723665 29.61128179645823 27.497560916489416 4 12.869121179006223 16.035246161584126 36.50403687626373 34.59159578314592 5 13.968328033462662 37.18509451141454 33.84014135681739 15.006436098305404 6 32.85025837366951 35.78020972793525 16.67590511557653 14.693626782818706 7 29.2025712466851 32.529057278543476 20.03343044755504 18.234941027216383 8 27.12759861733541 34.105793934106856 19.263807335674795 19.50280011288294 9 26.457278199004037 16.649710465502 19.918226510948994 36.97478482454497 10-11 25.360788512531123 26.15955608931837 26.735323128611952 21.74433226953855 12-13 26.388745484660152 23.378056098387894 27.321715865747386 22.911482551204564 14-15 23.049073052228337 24.88805039574879 25.498005353387164 26.564871198635704 16-17 22.5068434888731 28.27607183107657 26.20875133619988 23.008333343850452 18-19 22.672856163602205 26.70483741844169 28.5452814550315 22.0770249629246 20-21 23.25079183353285 25.59659788586946 28.724053238674234 22.42855704192345 22-23 22.97767120137967 25.377659288514202 28.87135947752999 22.773310032576134 24-25 23.046423840792162 25.73717521584971 28.67265048904396 22.543750454314168 26-27 22.334018649414134 26.032302566124255 28.765078257779464 22.86860052668215 28-29 22.514553308956238 26.577565564985427 28.43591117016631 22.47196995589203 30-31 22.58731881002955 25.94970581953734 28.321780361074772 23.141195009358338 32-33 22.34517228322605 26.02784298876688 28.3352013126341 23.291783415372965 34-35 23.196141078136403 26.052846873713403 28.08587913581677 22.66513291233342 36-37 23.249684686424214 25.57605146381977 28.23924911324761 22.935014736508403 38-39 22.696173639867474 26.033083922705586 28.213298580055362 23.057443857371577 40-41 22.946554259119146 26.002590293063495 28.27076234464929 22.78009310316806 42-43 22.763323115442812 25.985479574104524 27.86028790978305 23.39090940066961 44-45 22.786060887206077 26.055526474794217 28.040233752113124 23.118178885886586 46-47 23.450911399338427 26.44578868256951 27.31731942707354 22.785980491018524 48-49 23.10441410631941 26.598002005264576 27.098207479253183 23.199376409162827 50-51 22.96016670083219 26.856172565857833 27.32210280557864 22.861557927731333 52-53 23.495713002029696 26.534395334962507 26.970404451539714 22.999487211468082 54-55 23.023841678131035 26.750691017404804 27.00342037838725 23.222046926076914 56-57 23.17778977379268 26.834084261993972 26.874741162423938 23.113384801789408 58-59 23.253110977763267 26.365375676713683 26.98297863535359 23.398534710169468 60-61 22.693275454349767 26.741094759675793 27.544173227134383 23.021456558840057 62-63 22.763211422122488 26.783974787990378 27.557894449945948 22.89491933994119 64-65 22.581346840124496 26.967411804546533 27.22238007734845 23.228861277980524 66-67 22.64342638530684 26.809062599365074 27.09281139436641 23.454699620961676 68-69 22.97262035626646 26.451199853027717 27.42095445171567 23.155225338990153 70-71 23.027340171614856 26.58387409863337 27.004356237353612 23.384429492398162 72-73 22.88840165390703 26.677049011532127 27.038664518525696 23.395884816035146 74-75 22.690869830431307 27.42046569873865 26.772238776664437 23.116425694165603 76-77 23.100415805768176 26.68953589978258 26.896101057409666 23.313947237039574 78-79 22.92855323757695 26.944223382094933 26.93748424063127 23.18973913969685 80-81 23.27823729699894 26.99634271886585 26.58768426793679 23.137735716198414 82-83 23.14304641611094 26.91995634096946 26.840436337941085 23.096560904978517 84-85 22.89297476575992 27.19150609079724 26.717272752047172 23.198246391395667 86-87 23.338119438991033 27.127932856573285 26.47216909000066 23.06177861443503 88-89 23.004782394730533 27.00263725725246 26.251511060076467 23.74106928794054 90-91 22.89034245066343 26.874239595086667 26.6297239110295 23.6056940432204 92-93 23.20062285719333 26.58984424784568 26.855139013498185 23.354393881462805 94-95 22.926553022158558 26.332531825610495 27.113345709258624 23.62756944297232 96-97 22.9373242152403 26.1455412000105 27.259408333484764 23.65772625126444 98-99 22.79006692701833 26.699531584065628 26.57202270425335 23.93837878466269 100-101 23.238631230597402 25.540219373353096 27.35093528672823 23.87021410932127 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2851.0 1 1578.0 2 304.5 3 293.0 4 271.5 5 269.5 6 372.5 7 749.5 8 1295.5 9 1586.5 10 1514.0 11 1424.0 12 1566.0 13 1699.5 14 1779.5 15 1969.0 16 2132.0 17 2247.0 18 2432.0 19 2678.0 20 3032.5 21 3516.0 22 4146.0 23 5029.5 24 6095.5 25 7581.0 26 9643.0 27 12113.0 28 14233.5 29 16375.5 30 19245.5 31 23399.5 32 29411.5 33 32219.5 34 32424.5 35 34217.0 36 37609.0 37 42219.0 38 46559.0 39 49396.0 40 52249.5 41 56318.5 42 58202.5 43 59940.0 44 63419.0 45 67707.5 46 70965.0 47 75901.5 48 82769.0 49 82113.0 50 76685.5 51 73124.5 52 72623.5 53 74431.5 54 75749.5 55 71918.0 56 65553.5 57 60369.0 58 56301.0 59 50322.5 60 41113.0 61 34175.0 62 28516.0 63 21298.5 64 14653.5 65 10808.5 66 8095.5 67 6133.5 68 4737.0 69 3604.0 70 2771.5 71 1919.5 72 1321.5 73 997.0 74 732.5 75 545.0 76 374.0 77 235.0 78 161.0 79 111.0 80 74.0 81 52.5 82 39.5 83 23.5 84 14.5 85 12.0 86 14.5 87 12.5 88 8.0 89 9.5 90 7.5 91 4.0 92 4.5 93 3.5 94 2.0 95 1.5 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2941612023536926 2 0.2555777295792185 3 0.25457032820129494 4 0.25497328875246433 5 0.2553258792347376 6 0.2243482868635868 7 0.22550679844819893 8 0.2263127195505378 9 0.23739413470769744 10-11 0.2426578069073483 12-13 0.2365630285709105 14-15 0.22686679030839577 16-17 0.22205644872881056 18-19 0.21195724991512643 20-21 0.21671722142581548 22-23 0.2142994581187988 24-25 0.21649055611578266 26-27 0.2128135410863615 28-29 0.22590975899936835 30-31 0.2376963551210745 32-33 0.22948603389099714 34-35 0.22895714816758725 36-37 0.21868165411276652 38-39 0.20661802261213136 40-41 0.20465358992518032 42-43 0.21676759149471164 44-45 0.21321650163753092 46-47 0.2228119997622533 48-49 0.22767271141073464 50-51 0.23935856739464847 52-53 0.24970961655281354 54-55 0.22822678216859266 56-57 0.20613950695761762 58-59 0.1941766155947748 60-61 0.19797955579643642 62-63 0.20289063751381398 64-65 0.19558697752386786 66-67 0.2002713939312126 68-69 0.20047287420679732 70-71 0.20193360620478656 72-73 0.2044017395806994 74-75 0.20815430971346482 76-77 0.21825350852714898 78-79 0.21868165411276652 80-81 0.2110254036405471 82-83 0.20367137358170478 84-85 0.19100330125431544 86-87 0.1952343870415946 88-89 0.20556025116531154 90-91 0.2036210035128086 92-93 0.2071972784044374 94-95 0.20349507834056818 96-97 0.2110505886749952 98-99 0.21495426901444914 100-101 0.19820622110646924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1985306.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.22714606964951 #Duplication Level Percentage of deduplicated Percentage of total 1 70.17877019122136 32.44164260617954 2 14.551094397700917 13.453111323915579 3 5.8311340941300704 8.086700625631924 4 2.953385355912511 5.461063049909258 5 1.766784217840383 4.083669605582942 6 1.0379987583561554 2.8790232133586886 7 0.7257379215331465 2.348415503489762 8 0.5039894863628733 1.8638396482931339 9 0.38791117078997905 1.6138823718741406 >10 1.8936698861865733 15.517203146209932 >50 0.11276062425530568 3.593166552415708 >100 0.0490299082237993 4.176439724289685 >500 0.004139317246515738 1.3361183339576173 >1k 0.003485740839171147 2.8317611603392083 >5k 1.0892940122409834E-4 0.31396313455286584 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6235 0.31405737956768376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4653 0.23437193057392666 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3833 0.19306847407905883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3223 0.16234273205238892 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3184 0.16037829936543788 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2234 0.11252673391406665 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2221 0.1118719230184163 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2168 0.10920230936691876 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2149 0.10824527805789133 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2080 0.10476974330405489 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1993 0.1003875473100872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0074013779236048E-4 2 0.0 0.0 0.0 0.0 1.0074013779236048E-4 3 0.0 0.0 0.0 0.0 1.0074013779236048E-4 4 0.0 0.0 0.0 0.0 1.0074013779236048E-4 5 5.037006889618024E-5 0.0 0.0 0.0 1.0074013779236048E-4 6 5.037006889618024E-5 0.0 0.0 0.0 1.5111020668854072E-4 7 1.0074013779236048E-4 0.0 0.0 0.0 1.5111020668854072E-4 8 1.0074013779236048E-4 0.0 0.0 0.0 1.5111020668854072E-4 9 1.0074013779236048E-4 0.0 0.0 5.037006889618024E-5 1.5111020668854072E-4 10-11 1.0074013779236048E-4 0.0 0.0 5.037006889618024E-5 1.5111020668854072E-4 12-13 1.0074013779236048E-4 0.0 0.0 5.037006889618024E-5 2.518503444809012E-4 14-15 1.0074013779236048E-4 0.0 0.0 5.037006889618024E-5 2.518503444809012E-4 16-17 1.0074013779236048E-4 0.0 0.0 5.037006889618024E-5 2.518503444809012E-4 18-19 1.0074013779236048E-4 0.0 0.0 5.037006889618024E-5 2.518503444809012E-4 20-21 1.0074013779236048E-4 0.0 0.0 5.037006889618024E-5 2.518503444809012E-4 22-23 1.0074013779236048E-4 0.0 0.0 1.7629524113663082E-4 3.0222041337708144E-4 24-25 1.5111020668854072E-4 0.0 0.0 3.0222041337708144E-4 3.0222041337708144E-4 26-27 1.5111020668854072E-4 0.0 0.0 5.540707578579826E-4 3.0222041337708144E-4 28-29 2.0148027558472095E-4 0.0 0.0 0.001082956481267875 3.0222041337708144E-4 30-31 2.518503444809012E-4 0.0 0.0 0.001989617721399119 3.0222041337708144E-4 32-33 2.518503444809012E-4 0.0 0.0 0.003324424547147896 3.2740544782517154E-4 34-35 2.770353789289913E-4 0.0 0.0 0.00428145585617532 3.5259048227326165E-4 36-37 3.0222041337708144E-4 0.0 0.0 0.0051629320618584735 3.5259048227326165E-4 38-39 3.0222041337708144E-4 0.0 0.0 0.006271073577574439 3.5259048227326165E-4 40-41 3.0222041337708144E-4 0.0 0.0 0.00883994709127963 3.5259048227326165E-4 42-43 3.0222041337708144E-4 0.0 0.0 0.013751028808657206 3.777755167213518E-4 44-45 3.5259048227326165E-4 0.0 0.0 0.021432464315324688 4.029605511694419E-4 46-47 3.5259048227326165E-4 0.0 0.0 0.03933902380791676 4.029605511694419E-4 48-49 3.5259048227326165E-4 0.0 0.0 0.05339227302995105 4.029605511694419E-4 50-51 3.5259048227326165E-4 0.0 0.0 0.07354030058842315 4.029605511694419E-4 52-53 3.5259048227326165E-4 0.0 0.0 0.10255346027262296 5.037006889618024E-4 54-55 3.5259048227326165E-4 0.0 0.0 0.13670436698423316 5.037006889618024E-4 56-57 3.5259048227326165E-4 0.0 0.0 0.17546413499984387 5.037006889618024E-4 58-59 3.5259048227326165E-4 0.0 0.0 0.22331570045121507 5.037006889618024E-4 60-61 3.5259048227326165E-4 0.0 0.0 0.28786494374167004 5.037006889618024E-4 62-63 3.5259048227326165E-4 0.0 0.0 0.36384819267155794 5.037006889618024E-4 64-65 4.029605511694419E-4 0.0 0.0 0.430361868648964 5.037006889618024E-4 66-67 4.029605511694419E-4 0.0 0.0 0.5389093671202323 5.037006889618024E-4 68-69 4.029605511694419E-4 0.0 0.0 0.6810033314763568 5.037006889618024E-4 70-71 4.029605511694419E-4 0.0 0.0 0.7817686543031653 5.037006889618024E-4 72-73 4.029605511694419E-4 0.0 0.0 0.9569809389585283 5.288857234098925E-4 74-75 4.029605511694419E-4 0.0 0.0 1.1129266722611022 5.540707578579826E-4 76-77 4.029605511694419E-4 0.0 0.0 1.2506888106921552 5.540707578579826E-4 78-79 4.029605511694419E-4 0.0 0.0 1.4314921729949943 5.540707578579826E-4 80-81 4.533306200656221E-4 0.0 0.0 1.619246604805506 5.540707578579826E-4 82-83 4.533306200656221E-4 0.0 0.0 1.8385075147105785 5.540707578579826E-4 84-85 4.533306200656221E-4 0.0 0.0 2.1425916206368187 5.540707578579826E-4 86-87 4.533306200656221E-4 0.0 0.0 2.523817487077559 6.044408267541629E-4 88-89 4.533306200656221E-4 0.0 0.0 2.963850408954589 6.548108956503431E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11675 0.0 25.411142 1 GTACATG 7915 0.0 24.387745 1 TACATGG 7880 0.0 24.360004 2 GGTATCA 8620 0.0 24.3236 1 TAAGGTA 695 0.0 23.927343 4 GTGTAGG 570 0.0 23.35499 1 GTACGCT 150 3.0288942E-5 22.17211 3 ACATGGG 8180 0.0 22.013351 3 TAAGGTG 1155 0.0 21.802992 5 GAGTACT 6720 0.0 21.629314 12-13 ATCAACG 13820 0.0 21.28055 3 TCAACGC 13870 0.0 21.170116 4 TATCAAC 14015 0.0 21.12113 2 CAACGCA 13915 0.0 20.999748 5 AGTACTT 7120 0.0 20.84782 12-13 GTACTTT 6920 0.0 20.831535 14-15 AACGCAG 14170 0.0 20.585194 6 GTAAGGT 1115 0.0 20.45397 4 AGAGTAC 10875 0.0 20.39962 10-11 GGACCGA 430 0.0 19.886597 6 >>END_MODULE