FastQCFastQC Report
Thu 26 May 2016
SRR937223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985306
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76220.38392066512668577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58390.2941108322847964No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41500.20903578591914798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.2056609913031039No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28570.14390728683638693No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24500.12340666879564158No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24160.12169408645317145No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23750.11962891362842806No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23010.11590152853011072No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22940.11554893804783747No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20420.10285568068600004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT804.4960078E-429.6861443
TGTACGC1354.229405E-728.1468642
GTAAGGT10750.027.835944
TAAGGTG11750.027.083875
AGGTAAG10400.024.6623362
GTATCAA132150.024.0191781
GGTAAGG11950.023.8482863
GGTATCA99650.022.9359441
GTACATG72550.022.2029721
TACATGG72900.022.0223162
GAGTACT78900.021.94512612-13
AGGCGAC7800.021.313135
GTACTTT81150.021.15892414-15
ACATGGG74600.020.5017453
ATCAACG154250.020.4463923
TCAACGC154100.020.4354724
TATCAAC155400.020.3562152
CAACGCA154900.020.2686045
CATGGGG42000.020.1300354
AGAGTAC116700.020.06751610-11
Produced by FastQC (version 0.11.5)