##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937223_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985306 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3163336029811 33.0 31.0 34.0 31.0 34.0 2 32.51635516137059 34.0 31.0 34.0 31.0 34.0 3 32.58791692565277 34.0 31.0 34.0 31.0 34.0 4 36.189861915493125 37.0 35.0 37.0 35.0 37.0 5 36.121052875476124 37.0 35.0 37.0 35.0 37.0 6 36.16038837337922 37.0 36.0 37.0 35.0 37.0 7 36.10428971654748 37.0 35.0 37.0 35.0 37.0 8 36.07648191261196 37.0 35.0 37.0 35.0 37.0 9 37.79955331822903 39.0 38.0 39.0 35.0 39.0 10-11 37.71526731899264 39.0 37.0 39.0 35.0 39.0 12-13 37.61730811270404 39.0 37.0 39.0 35.0 39.0 14-15 38.89088860860744 40.0 38.0 41.0 35.5 41.0 16-17 38.83723390751854 40.0 38.0 41.0 35.0 41.0 18-19 38.81289584577894 40.0 38.0 41.0 35.0 41.0 20-21 38.69148811316744 40.0 38.0 41.0 34.5 41.0 22-23 38.636313998950286 40.0 38.0 41.0 34.0 41.0 24-25 38.655007842619725 40.0 38.0 41.0 34.0 41.0 26-27 38.50038079772085 40.0 38.0 41.0 34.0 41.0 28-29 38.36326616652546 40.0 38.0 41.0 34.0 41.0 30-31 38.415638193809926 40.0 38.0 41.0 34.0 41.0 32-33 38.332346499733546 40.0 38.0 41.0 34.0 41.0 34-35 38.209902402954505 40.0 38.0 41.0 34.0 41.0 36-37 38.03890735231748 40.0 37.5 41.0 33.5 41.0 38-39 37.915315825368985 40.0 37.0 41.0 33.0 41.0 40-41 37.734196390883824 40.0 37.0 41.0 33.0 41.0 42-43 37.53821300091774 40.0 37.0 41.0 33.0 41.0 44-45 37.2567944185934 40.0 36.5 41.0 32.0 41.0 46-47 36.92685938590827 39.5 36.0 41.0 31.0 41.0 48-49 36.59812240531183 39.0 36.0 41.0 31.0 41.0 50-51 36.33646425286581 39.0 35.5 41.0 30.0 41.0 52-53 36.09026291161161 39.0 35.0 40.0 30.0 41.0 54-55 35.85939346377838 39.0 35.0 40.0 29.0 41.0 56-57 35.52645838978978 38.0 35.0 40.0 28.5 41.0 58-59 35.23121120875069 38.0 34.5 40.0 28.0 41.0 60-61 34.8529581837762 37.5 34.0 40.0 27.5 41.0 62-63 34.45855223325775 37.0 34.0 40.0 26.5 41.0 64-65 34.017020549980714 36.0 33.5 39.0 26.0 40.5 66-67 33.56296082316781 36.0 33.0 39.0 26.0 40.0 68-69 33.10214999602076 35.0 32.5 38.5 25.0 40.0 70-71 32.637288407933084 35.0 32.0 37.5 24.5 40.0 72-73 32.167698329627775 35.0 31.5 37.0 23.0 39.0 74-75 31.708735328458182 35.0 31.0 36.5 22.0 39.0 76-77 30.415384076812337 33.5 29.5 35.0 20.5 37.0 78-79 31.077477728874037 34.0 31.0 35.0 21.0 37.0 80-81 30.919861724086864 34.0 31.0 35.0 20.0 37.0 82-83 30.55313009682135 34.0 31.0 35.0 19.0 36.5 84-85 30.154935057870173 34.0 30.0 35.0 18.0 36.0 86-87 29.827238471046783 34.0 30.0 35.0 14.0 36.0 88-89 29.39599739284523 34.0 29.5 35.0 7.5 35.0 90-91 28.87100326095826 34.0 29.0 35.0 3.5 35.0 92-93 28.40632401251998 33.0 29.0 35.0 2.0 35.0 94-95 27.93933831862695 33.0 27.5 35.0 2.0 35.0 96-97 27.29207008894347 33.0 27.0 35.0 2.0 35.0 98-99 26.326161810824125 32.5 25.0 34.0 2.0 35.0 100-101 23.32770943119096 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20514511958691628 1101 2 0.19663582741586794 1101 3 0.18203154332987026 1101 4 0.11517805413863158 1101 5 0.0928320880622664 1101 6 0.17211882671741563 1101 7 0.1899911367677447 1101 8 0.188279668001897 1101 9 0.2317500763836975 1101 10-11 0.19620370215758953 1101 12-13 0.25745633539472834 1101 14-15 0.30009109302462633 1101 16-17 0.27494710841336456 1101 18-19 0.20267082395937308 1101 20-21 0.35576088534469363 1101 22-23 0.24604946593461108 1101 24-25 0.25149103058902966 1101 26-27 0.25616924017638354 1101 28-29 0.12036378998897135 1101 30-31 0.32908933173161614 1101 32-33 0.2719035209552203 1101 34-35 0.32337185708077243 1101 36-37 0.36852838941724286 1101 38-39 0.34136247853090396 1101 40-41 0.28113237757737153 1101 42-43 0.34143195538583626 1101 44-45 0.23556149171136553 1101 46-47 0.2555859345859304 1101 48-49 0.2409381493192413 1101 50-51 0.2007350697866528 1101 52-53 0.2921889384932541 1101 54-55 0.3847381453368186 1101 56-57 0.5143503933240439 1101 58-59 0.34062197571831376 1101 60-61 0.48406046776978684 1101 62-63 0.5816532813329758 1101 64-65 0.4645753829567383 1101 66-67 0.3771165265489955 1101 68-69 0.3526115599992252 1101 70-71 0.41147596946697007 1101 72-73 0.4693228321808789 1101 74-75 0.43594621944444967 1101 76-77 0.4955430673990229 1101 78-79 0.5479147022080326 1101 80-81 0.37504318453133934 1101 82-83 0.3495312518174387 1101 84-85 0.5076505116552745 1101 86-87 0.6186396101468468 1101 88-89 1.0645317293151209 1101 90-91 1.0628170441978178 1101 92-93 1.0291629490696614 1101 94-95 0.9965909705497857 1101 96-97 1.068136979023631 1101 98-99 1.2226329939587544 1101 100-101 1.9935023573114314 1102 1 0.20704958086637504 1102 2 0.2172482011532395 1102 3 0.1884288497271811 1102 4 0.10325675784233113 1102 5 0.04926096011613623 1102 6 0.10115923075781552 1102 7 0.15560561488221936 1102 8 0.08607007204229689 1102 9 0.2257315578651813 1102 10-11 0.1814993671407592 1102 12-13 0.1085663943172932 1102 14-15 0.16813612669466949 1102 16-17 0.11185788282414677 1102 18-19 0.2589839552724982 1102 20-21 0.2931346227184335 1102 22-23 0.2109642386618802 1102 24-25 0.04544094641394736 1102 26-27 0.20115556172520854 1102 28-29 0.1466158943660716 1102 30-31 0.22037194957673734 1102 32-33 0.32719602937272896 1102 34-35 0.3257655860369937 1102 36-37 0.33353259817145897 1102 38-39 0.2453081856016084 1102 40-41 0.3464311991261937 1102 42-43 0.38764933754071507 1102 44-45 0.26038788060025553 1102 46-47 0.33550491606741417 1102 48-49 0.5004814994875986 1102 50-51 0.5115726118742003 1102 52-53 0.4896952516245676 1102 54-55 0.4867109988721694 1102 56-57 0.4382929438290972 1102 58-59 0.4965037097250544 1102 60-61 0.601474188308508 1102 62-63 0.5348887611309578 1102 64-65 0.6700941119129666 1102 66-67 0.7413473767173429 1102 68-69 0.46935714080731117 1102 70-71 0.46160749303599147 1102 72-73 0.6027982109687571 1102 74-75 0.5051697042929284 1102 76-77 0.4653347340656886 1102 78-79 0.47907000523833787 1102 80-81 0.41312714917780724 1102 82-83 0.40296138649757296 1102 84-85 0.5894160840458476 1102 86-87 0.639751773446509 1102 88-89 0.6828976804935749 1102 90-91 0.8718027344335049 1102 92-93 0.9439567201134338 1102 94-95 1.065483016004329 1102 96-97 1.262960211346865 1102 98-99 1.610180342443602 1102 100-101 2.4691178876144626 1103 1 0.22325570788695615 1103 2 0.2454553750467099 1103 3 0.20497056704898142 1103 4 0.11547660677744886 1103 5 0.10586315404652424 1103 6 0.1089460514161118 1103 7 0.03709228695210243 1103 8 0.12470446575655814 1103 9 0.09695493577503811 1103 10-11 0.10241930669228339 1103 12-13 0.20472567940441877 1103 14-15 0.21064774923507912 1103 16-17 0.2278356395894221 1103 18-19 0.18598145628560303 1103 20-21 0.2406657474876681 1103 22-23 0.1586701940178159 1103 24-25 0.1503470374130842 1103 26-27 0.1904071742471629 1103 28-29 0.16911592250781382 1103 30-31 0.08861687562023945 1103 32-33 0.17783020358138657 1103 34-35 0.2766318592820767 1103 36-37 0.2913143533157694 1103 38-39 0.26495604795471195 1103 40-41 0.25179614126676597 1103 42-43 0.2365601816804812 1103 44-45 0.3046045410757401 1103 46-47 0.3733910911540832 1103 48-49 0.38384030109955347 1103 50-51 0.262615263501921 1103 52-53 0.33319830781800874 1103 54-55 0.3007464827976065 1103 56-57 0.4227435747669972 1103 58-59 0.3683567591644632 1103 60-61 0.35726364866242477 1103 62-63 0.29345264570478236 1103 64-65 0.4659563608886046 1103 66-67 0.4338435916527317 1103 68-69 0.4431927057809659 1103 70-71 0.2968541132124969 1103 72-73 0.1844757544196156 1103 74-75 0.4605840351948167 1103 76-77 0.45268150226776527 1103 78-79 0.43398763435265764 1103 80-81 0.5865144161641602 1103 82-83 0.5386622847820526 1103 84-85 0.549839645439878 1103 86-87 0.7946914234757791 1103 88-89 0.8934531563141874 1103 90-91 1.0521420675867326 1103 92-93 1.052816022648443 1103 94-95 1.1093479046755874 1103 96-97 1.37017181475089 1103 98-99 1.8822726670642496 1103 100-101 2.547179920461506 1104 1 0.0564030429983049 1104 2 0.13319091149570284 1104 3 0.09285322073570512 1104 4 0.0766257465269149 1104 5 0.049035267904059765 1104 6 0.0397736279118206 1104 7 0.07111770180519983 1104 8 0.09297478037027673 1104 9 0.11917804537107202 1104 10-11 0.09879321706146271 1104 12-13 0.088316713099168 1104 14-15 0.08993210093494497 1104 16-17 0.11639047894089316 1104 18-19 0.040693376214591126 1104 20-21 0.12992499818455627 1104 22-23 0.13409460973470289 1104 24-25 0.04700873712158682 1104 26-27 0.16526413776138327 1104 28-29 -0.017431537162792665 1104 30-31 0.10695223706976975 1104 32-33 0.09485292899126563 1104 34-35 0.02278316725940499 1104 36-37 -0.042078177191797295 1104 38-39 0.13115011800913834 1104 40-41 0.05766303659565608 1104 42-43 0.13016499535650183 1104 44-45 0.1625898195441451 1104 46-47 0.18238212610984306 1104 48-49 0.2891766636468702 1104 50-51 0.3072401250540864 1104 52-53 0.21148506453442906 1104 54-55 0.2248273328269974 1104 56-57 0.2643287840202504 1104 58-59 0.12525743331169537 1104 60-61 0.20738410965778087 1104 62-63 0.2056523776711856 1104 64-65 0.10503749464410106 1104 66-67 0.20575933130565716 1104 68-69 0.226546810667152 1104 70-71 0.2934718178613238 1104 72-73 0.4325388859291124 1104 74-75 0.3867388879970157 1104 76-77 0.36932312397610545 1104 78-79 0.35844923780445015 1104 80-81 0.496172172430132 1104 82-83 0.45410980491084274 1104 84-85 0.417376335428127 1104 86-87 0.4647663061497802 1104 88-89 0.6589994201318206 1104 90-91 0.7427884937237224 1104 92-93 0.8575038784006921 1104 94-95 0.8091646386308753 1104 96-97 1.0260302683525673 1104 98-99 1.1719198869337646 1104 100-101 1.8804071192161942 1105 1 0.12454358849462466 1105 2 0.11192964317157816 1105 3 0.12258624352660519 1105 4 0.10065619972070294 1105 5 0.08911876157180387 1105 6 0.01896576025603025 1105 7 0.06094101274071306 1105 8 0.07360812578993858 1105 9 0.143976361560334 1105 10-11 0.17658551295221514 1105 12-13 0.13906024010776008 1105 14-15 0.15511846450696254 1105 16-17 0.19227988576346178 1105 18-19 0.21352853501915803 1105 20-21 0.27787244551919343 1105 22-23 0.08421824961435931 1105 24-25 0.19492722082082992 1105 26-27 0.1080499603885201 1105 28-29 0.17332546991369213 1105 30-31 0.10177558611533044 1105 32-33 0.2162704485063287 1105 34-35 0.19632434189814774 1105 36-37 0.14800351985946492 1105 38-39 0.11567765687321696 1105 40-41 0.16892052658087664 1105 42-43 0.0618557856826456 1105 44-45 0.12247289792127702 1105 46-47 0.2099215346201433 1105 48-49 0.27923897901730754 1105 50-51 0.2150302541164848 1105 52-53 0.1405322153729074 1105 54-55 0.10130537654030292 1105 56-57 0.17296920266456084 1105 58-59 0.078135340699923 1105 60-61 0.3269887887004188 1105 62-63 0.43309509554904935 1105 64-65 0.36951882235457134 1105 66-67 0.33782882660887026 1105 68-69 0.3961707080618382 1105 70-71 0.36547530656387295 1105 72-73 0.38202009930943603 1105 74-75 0.38555649658070124 1105 76-77 0.39189603121733896 1105 78-79 0.5028656259955646 1105 80-81 0.3787848447828175 1105 82-83 0.377492931742168 1105 84-85 0.5251356497957254 1105 86-87 0.8109274811587355 1105 88-89 0.9366839190337082 1105 90-91 0.9943904767527734 1105 92-93 0.9345688168311881 1105 94-95 1.0255796156614068 1105 96-97 1.2298831940608395 1105 98-99 1.3719112849518282 1105 100-101 1.8974861910296745 1106 1 -0.04288593676125885 1106 2 0.05971408076649709 1106 3 0.00787820711428111 1106 4 -0.0030378271122728506 1106 5 -0.002197815382032786 1106 6 -0.017186188414449077 1106 7 -0.016728131138293634 1106 8 -0.1282140630832771 1106 9 -0.09236182734882448 1106 10-11 -0.28723681520801847 1106 12-13 -0.41350039468164823 1106 14-15 -0.14980802788615932 1106 16-17 -0.0877132172819799 1106 18-19 -0.04569709649026521 1106 20-21 -0.009670913185345853 1106 22-23 -0.011838017852682015 1106 24-25 0.06437112858586147 1106 26-27 0.10539463288426987 1106 28-29 -0.05394086486043648 1106 30-31 -0.048054348914007505 1106 32-33 0.044407664203532704 1106 34-35 -0.41655824812171005 1106 36-37 -0.043545576572093125 1106 38-39 0.16920240503328188 1106 40-41 0.0716110128274039 1106 42-43 0.005135298807076083 1106 44-45 -0.02362841317405895 1106 46-47 0.002404640451324269 1106 48-49 0.18930246694426245 1106 50-51 0.24040397020458926 1106 52-53 0.08703599858836952 1106 54-55 0.15810606578644837 1106 56-57 0.11253997395525772 1106 58-59 0.09738177540126713 1106 60-61 0.11536000660824186 1106 62-63 0.19092965400915318 1106 64-65 0.2672441752899246 1106 66-67 0.2026533474165575 1106 68-69 0.3030709201375785 1106 70-71 0.4999496328190318 1106 72-73 0.27847490740785474 1106 74-75 0.22308143345222575 1106 76-77 0.14361375481795946 1106 78-79 0.1641841574609515 1106 80-81 0.22966650921789622 1106 82-83 0.33923437906920384 1106 84-85 0.4049799179097775 1106 86-87 0.46649592950121743 1106 88-89 0.6964104222103522 1106 90-91 0.4137826011945016 1106 92-93 0.6696118610166266 1106 94-95 0.3757657435718329 1106 96-97 0.6072150162047834 1106 98-99 0.8169909706124514 1106 100-101 1.1090179215137645 1107 1 0.11074554661017544 1107 2 0.10820118238958543 1107 3 0.1261854928696735 1107 4 0.05597025883758988 1107 5 0.04205864006213034 1107 6 0.06446622119503331 1107 7 0.052307185016267965 1107 8 0.06963142628291052 1107 9 0.10676049677193333 1107 10-11 0.0718539131172875 1107 12-13 0.027454655372956438 1107 14-15 0.04403068029218815 1107 16-17 0.10355830458887283 1107 18-19 0.13952135731311444 1107 20-21 0.1045267073219378 1107 22-23 0.19444653376495324 1107 24-25 0.08168570045411627 1107 26-27 0.06691521237734577 1107 28-29 0.18882999390532973 1107 30-31 0.19640376375666335 1107 32-33 0.20552086303317907 1107 34-35 0.15883499017252234 1107 36-37 0.2796062190843003 1107 38-39 0.17059262450140267 1107 40-41 0.18315744180098648 1107 42-43 0.23621368015148647 1107 44-45 0.31620221799115455 1107 46-47 0.38629992257484247 1107 48-49 0.46447163917106593 1107 50-51 0.4656991726124957 1107 52-53 0.44090499780015335 1107 54-55 0.4759882091351102 1107 56-57 0.4363499594956366 1107 58-59 0.45800180099605114 1107 60-61 0.5592535639435923 1107 62-63 0.5426377528279005 1107 64-65 0.6121126624273003 1107 66-67 0.6012797055755215 1107 68-69 0.6160653445141122 1107 70-71 0.6077043205787547 1107 72-73 0.5992101005930692 1107 74-75 0.5787947495317205 1107 76-77 0.5077406634792503 1107 78-79 0.6109343393233289 1107 80-81 0.5076763315736521 1107 82-83 0.5281375567903055 1107 84-85 0.5158306227493732 1107 86-87 0.8572850022086378 1107 88-89 1.0423508696543564 1107 90-91 1.1545543143112944 1107 92-93 1.1293892203570266 1107 94-95 1.1274524389313498 1107 96-97 1.2954286137911524 1107 98-99 1.4704223830262784 1107 100-101 2.1375652879499256 1108 1 0.00990116873951763 1108 2 0.08642561720565567 1108 3 0.0027945477135631336 1108 4 -0.004098981517714151 1108 5 -0.07915757401360679 1108 6 -0.007557832700626932 1108 7 0.0249873398673941 1108 8 -0.010444055721826828 1108 9 -0.042208796282068306 1108 10-11 -0.05588135703953867 1108 12-13 -0.00996345508540486 1108 14-15 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76-77 -0.09627458464590433 2316 78-79 0.11553628517052417 2316 80-81 0.07388518848697956 2316 82-83 0.1753111957792015 2316 84-85 0.17210375622215324 2316 86-87 0.20439901067897281 2316 88-89 0.3874216130004591 2316 90-91 0.556872399616811 2316 92-93 0.705949462219813 2316 94-95 0.5235630514991385 2316 96-97 0.6778653819338523 2316 98-99 0.7669352825792259 2316 100-101 1.2853809332809156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 13.0 5 29.0 6 57.0 7 177.0 8 395.0 9 742.0 10 1276.0 11 2238.0 12 3379.0 13 5015.0 14 6473.0 15 9143.0 16 12197.0 17 17008.0 18 15475.0 19 10275.0 20 9516.0 21 9514.0 22 10042.0 23 10773.0 24 12748.0 25 14960.0 26 18476.0 27 23418.0 28 28960.0 29 36308.0 30 45798.0 31 58489.0 32 76273.0 33 103177.0 34 144144.0 35 213231.0 36 330741.0 37 453541.0 38 282304.0 39 18991.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.05674391417615 18.820476384836713 15.281653590522692 22.841126110464444 2 15.869063815447818 20.039913302906406 38.86852535859766 25.22249752304812 3 19.611838175072254 23.442431544557866 29.888792961891014 27.056937318478862 4 13.436719578745041 16.44023641695537 36.085369207568 34.03767479673159 5 14.059003974230466 37.20458975766764 33.80664789526971 14.92975837283218 6 33.37727282343377 35.73071355246999 16.285146974824034 14.606866649272202 7 29.49666197553425 32.58631163155705 19.79296894282292 18.124057450085783 8 27.60828809261645 33.803907306984414 19.002410711497372 19.585393888901763 9 26.775620483693697 16.238705771301753 19.670771155680786 37.314902589323765 10-11 25.599655670209025 26.02921161775565 26.556737349305347 21.814395362729975 12-13 26.55791017477085 23.329443715858282 27.20812190412097 22.904524205249903 14-15 23.181892363192375 24.726742376238224 25.473151242176268 26.618214018393132 16-17 22.64046449262733 27.961281535440886 26.511051696816512 22.887202275115275 18-19 22.806962755363656 26.31941876970099 28.804275008487355 22.069343466447993 20-21 23.1977184339116 25.21589903405173 29.179072413631573 22.4073101184051 22-23 23.011999491258845 25.021753721428958 29.329053527376946 22.63719325993525 24-25 23.135354367732692 25.388687422351293 29.11109057129049 22.36486763862553 26-27 22.46715174044775 25.701329545425928 29.101782381697156 22.72973633242917 28-29 22.556279818550586 26.198566266538016 28.849560241311302 22.395593673600096 30-31 22.580222701690396 25.694334084772514 28.667131919257606 23.05831129427948 32-33 22.42464309595137 25.705780353371843 28.75493784952167 23.114638701155112 34-35 23.20435741392007 25.644610956698866 28.568341605777647 22.582690023603412 36-37 23.40459022669061 25.27969254094459 28.572990044350867 22.742727188013934 38-39 22.76403947102321 25.751918660336486 28.614401468389357 22.869640400250944 40-41 23.025216263890805 25.744142212837716 28.547110621737907 22.683530901533565 42-43 22.883848388717688 25.69601426597839 28.175005068905634 23.245132276398294 44-45 22.925130936994094 25.749179219727335 28.328529707762932 22.997160135515635 46-47 23.63333410567439 26.282421954096748 27.62322281804417 22.46102112218469 48-49 23.359245375775824 26.437561766296984 27.265771624122426 22.937421233804763 50-51 23.142981484970075 26.4954369754587 27.727614785831506 22.633966753739728 52-53 23.737435809611117 26.34209856923747 27.20944640747895 22.711019213672458 54-55 23.136307450841333 26.62481249741853 27.29554033484007 22.943339716900066 56-57 23.298297592411448 26.559381777922397 27.233887370511145 22.90843325915501 58-59 23.32038486762242 26.188179555191997 27.280378944102317 23.211056633083263 60-61 22.859826142670197 26.488460720916574 27.920481779634976 22.73123135677825 62-63 22.859070591636755 26.619296974874402 27.95198825772954 22.569644175759304 64-65 22.673557627892123 26.768845709427165 27.552603981451725 23.004992681228988 66-67 22.821620445412446 26.613857007433616 27.320901664529295 23.243620882624644 68-69 23.184763457119455 26.164404882672997 27.65765076013471 22.993180900072836 70-71 23.17257390044658 26.412905617572303 27.24224880194791 23.172271680033205 72-73 23.02787030493582 26.49902810805202 27.278632027453742 23.194469559558414 74-75 22.841441067523093 27.073030555491194 27.01986494777128 23.06566342921444 76-77 23.168846515348264 26.424692213694012 27.330245312309536 23.076215958648188 78-79 23.119383107692215 26.645741261044897 27.21575414570852 23.01912148555437 80-81 23.337913651598292 26.741343651809846 26.931918807478546 22.988823889113316 82-83 23.250962008987567 26.72434397144812 27.10499202009169 22.919701999472625 84-85 22.9170113874334 26.93675145223461 27.079777515013454 23.066459645318535 86-87 23.139203729802862 27.03495078340568 26.727441512794503 23.098403973996955 88-89 22.85161582144012 27.01533164157062 26.525054575969648 23.60799796101961 90-91 22.81710794461506 26.64883235283228 26.93689992743528 23.59715977511738 92-93 23.00493758444957 26.3542594064069 27.311780565234418 23.329022443909118 94-95 22.801422047785078 26.174050750866616 27.541421826156775 23.48310537519153 96-97 22.749389766615323 25.915299706946936 27.673366221630317 23.66194430480742 98-99 22.42034209404917 26.48773335240684 27.028708982311006 24.06321557123299 100-101 22.359855138682697 26.013502791587804 27.7084547605602 23.918187309169294 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 140.0 2 143.5 3 219.5 4 214.5 5 213.0 6 387.0 7 880.5 8 1518.5 9 1831.5 10 1740.0 11 1641.5 12 1722.5 13 1916.5 14 2123.5 15 2195.0 16 2343.5 17 2579.5 18 2828.0 19 3047.0 20 3296.5 21 3658.0 22 4212.0 23 5069.5 24 5996.0 25 7426.0 26 9387.0 27 11697.5 28 13836.0 29 16052.5 30 19000.5 31 23049.5 32 28984.0 33 31789.5 34 31725.5 35 33590.0 36 37336.5 37 41723.0 38 45772.0 39 48718.5 40 52181.0 41 56614.5 42 58273.5 43 59955.5 44 63203.5 45 67304.0 46 71158.5 47 76520.0 48 83705.5 49 82732.0 50 76493.5 51 73274.0 52 73385.0 53 75682.0 54 77388.0 55 72790.5 56 65488.5 57 60802.5 58 57372.5 59 51136.0 60 41315.5 61 34115.0 62 28752.0 63 21116.5 64 13985.0 65 10186.5 66 7504.5 67 5977.0 68 4704.0 69 3532.5 70 2678.0 71 1692.5 72 1156.5 73 952.5 74 703.0 75 517.5 76 356.5 77 203.0 78 125.0 79 69.5 80 37.5 81 25.0 82 16.5 83 9.5 84 7.0 85 3.0 86 2.0 87 2.0 88 2.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03908717346343586 2 1.511102066885407E-4 3 0.0 4 0.0 5 8.059211023388837E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005364412337443195 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.511102066885407E-4 22-23 6.799959300984332E-4 24-25 1.259251722404506E-4 26-27 0.0020651728247433896 28-29 0.003500719788284527 30-31 2.2666531003281105E-4 32-33 1.7629524113663082E-4 34-35 0.0 36-37 5.037006889618023E-5 38-39 7.555510334427035E-5 40-41 0.0 42-43 0.00823550626452547 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.037006889618023E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.511102066885407E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.7777551672135175E-4 84-85 1.7629524113663082E-4 86-87 0.0 88-89 0.0 90-91 0.009142167504656712 92-93 0.0011836966190602357 94-95 0.0 96-97 0.0 98-99 2.0148027558472093E-4 100-101 0.011836966190602356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1985306.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.896581109582804 #Duplication Level Percentage of deduplicated Percentage of total 1 68.01097931517053 29.854494698505412 2 15.518225911550163 13.623941248063822 3 6.271889731510602 8.25943548928844 4 3.108656853245285 5.458376308013683 5 1.8427782562992396 4.044583259730756 6 1.155295324195615 3.0428108942444747 7 0.7906250666894863 2.4294016157043044 8 0.5622522153404232 1.9744759979786803 9 0.4354887252748722 1.7204819536203522 >10 2.1143674524729548 16.406972072812298 >50 0.1213435456918168 3.647274648297991 >100 0.060148598079305636 4.813638961979669 >500 0.004262509648532099 1.3182629908869055 >1k 0.0034560889042152152 2.7251249061268052 >5k 2.30405926947681E-4 0.68072495474638 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7622 0.38392066512668577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5839 0.2941108322847964 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4150 0.20903578591914798 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4083 0.2056609913031039 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2857 0.14390728683638693 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2450 0.12340666879564158 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2416 0.12169408645317145 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2375 0.11962891362842806 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2301 0.11590152853011072 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2294 0.11554893804783747 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2042 0.10285568068600004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 5.037006889618024E-5 3 0.0 0.0 0.0 0.0 5.037006889618024E-5 4 0.0 0.0 0.0 0.0 5.037006889618024E-5 5 0.0 0.0 0.0 0.0 5.037006889618024E-5 6 0.0 0.0 0.0 0.0 5.037006889618024E-5 7 0.0 0.0 0.0 0.0 5.037006889618024E-5 8 0.0 0.0 0.0 0.0 5.037006889618024E-5 9 0.0 0.0 0.0 0.0 5.037006889618024E-5 10-11 0.0 0.0 0.0 0.0 5.037006889618024E-5 12-13 0.0 0.0 0.0 0.0 7.555510334427036E-5 14-15 0.0 0.0 0.0 0.0 1.0074013779236048E-4 16-17 0.0 0.0 0.0 0.0 1.5111020668854072E-4 18-19 0.0 0.0 0.0 0.0 2.0148027558472095E-4 20-21 0.0 0.0 0.0 0.0 2.0148027558472095E-4 22-23 0.0 0.0 0.0 1.259251722404506E-4 2.0148027558472095E-4 24-25 0.0 0.0 0.0 2.518503444809012E-4 2.0148027558472095E-4 26-27 0.0 0.0 0.0 5.037006889618024E-4 2.0148027558472095E-4 28-29 5.037006889618024E-5 0.0 0.0 9.570313090274244E-4 2.0148027558472095E-4 30-31 5.037006889618024E-5 0.0 0.0 0.0019140626180548488 2.0148027558472095E-4 32-33 5.037006889618024E-5 0.0 0.0 0.003223684409355535 2.518503444809012E-4 34-35 5.037006889618024E-5 0.0 0.0 0.00418071571838296 2.518503444809012E-4 36-37 1.0074013779236048E-4 0.0 0.0 0.005062191924066114 2.518503444809012E-4 38-39 1.0074013779236048E-4 0.0 0.0 0.00619551847423017 2.518503444809012E-4 40-41 1.0074013779236048E-4 0.0 0.0 0.008764391987935362 2.518503444809012E-4 42-43 1.5111020668854072E-4 0.0 0.0 0.013801398877553384 2.518503444809012E-4 44-45 1.5111020668854072E-4 0.0 0.0 0.021508019418668964 2.518503444809012E-4 46-47 2.2666531003281108E-4 0.0 0.0 0.03971679932463812 2.518503444809012E-4 48-49 2.518503444809012E-4 0.0 0.0 0.053870788684464765 3.0222041337708144E-4 50-51 2.518503444809012E-4 0.0 0.0 0.07424548155296967 3.0222041337708144E-4 52-53 2.518503444809012E-4 0.0 0.0 0.10335938137496184 3.0222041337708144E-4 54-55 2.518503444809012E-4 0.0 0.0 0.13786287856884533 3.0222041337708144E-4 56-57 2.518503444809012E-4 0.0 0.0 0.17695005203228117 3.0222041337708144E-4 58-59 2.518503444809012E-4 0.0 0.0 0.22507865286258139 3.0222041337708144E-4 60-61 2.518503444809012E-4 0.0 0.0 0.2895523410496921 3.0222041337708144E-4 62-63 2.518503444809012E-4 0.0 0.0 0.3656867001862685 3.5259048227326165E-4 64-65 2.518503444809012E-4 0.0 0.0 0.4328048169904287 4.029605511694419E-4 66-67 2.518503444809012E-4 0.0 0.0 0.5424604569774131 4.28145585617532E-4 68-69 2.518503444809012E-4 0.0 0.0 0.685813673055942 4.533306200656221E-4 70-71 2.518503444809012E-4 0.0 0.0 0.7874604720884337 5.540707578579826E-4 72-73 2.518503444809012E-4 0.0 0.0 0.9645616343274035 6.296258612022529E-4 74-75 2.518503444809012E-4 0.0 0.0 1.1222703200413437 7.051809645465233E-4 76-77 2.518503444809012E-4 0.0 0.0 1.2620976312971401 7.555510334427035E-4 78-79 2.518503444809012E-4 0.0 0.0 1.446804673939433 7.555510334427035E-4 80-81 2.518503444809012E-4 0.0 0.0 1.6372539044358905 7.555510334427035E-4 82-83 2.518503444809012E-4 0.0 0.0 1.8609725654382752 7.555510334427035E-4 84-85 2.518503444809012E-4 0.0 0.0 2.1712773748731937 7.555510334427035E-4 86-87 2.518503444809012E-4 0.0 0.0 2.560864672750699 7.807360678907937E-4 88-89 2.518503444809012E-4 0.0 0.0 3.013842702334048 8.059211023388838E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCT 80 4.4960078E-4 29.686144 3 TGTACGC 135 4.229405E-7 28.146864 2 GTAAGGT 1075 0.0 27.83594 4 TAAGGTG 1175 0.0 27.08387 5 AGGTAAG 1040 0.0 24.662336 2 GTATCAA 13215 0.0 24.019178 1 GGTAAGG 1195 0.0 23.848286 3 GGTATCA 9965 0.0 22.935944 1 GTACATG 7255 0.0 22.202972 1 TACATGG 7290 0.0 22.022316 2 GAGTACT 7890 0.0 21.945126 12-13 AGGCGAC 780 0.0 21.31313 5 GTACTTT 8115 0.0 21.158924 14-15 ACATGGG 7460 0.0 20.501745 3 ATCAACG 15425 0.0 20.446392 3 TCAACGC 15410 0.0 20.435472 4 TATCAAC 15540 0.0 20.356215 2 CAACGCA 15490 0.0 20.268604 5 CATGGGG 4200 0.0 20.130035 4 AGAGTAC 11670 0.0 20.067516 10-11 >>END_MODULE