FastQCFastQC Report
Thu 26 May 2016
SRR937222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943925
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.319816865362604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46280.2380750286147871No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37770.19429761950692542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.1664158853865247No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27260.14023174762400814No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22070.11353318672273878No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21530.11075530177347377No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21200.10905770541558961No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21170.10890337847396375No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21020.10813174376583458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG74750.025.6395021
TACATGG74550.025.3762252
GTATCAA118450.024.6919731
ACATGGG74650.023.240993
GGTATCA89600.022.8231811
GAGTACT63150.022.75459712-13
GTACTTT66100.021.6284914-15
TAAGGTA6050.021.2145394
TCAACGC136750.021.2044814
ATCAACG137500.021.121763
CAACGCA137250.021.0915135
TATCAAC138550.021.064612
AGAGTAC100050.021.03393610-11
CATGGGG41550.020.822154
AACGCAG140050.020.6914946
AGTACTT65050.020.66599312-13
AGGTAAG10500.019.9184252
GGTAAGG11000.019.877273
GTAAGGT11100.019.6997184
TAAGGTG11600.019.669175
Produced by FastQC (version 0.11.5)