FastQCFastQC Report
Thu 26 May 2016
SRR937222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943925
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75840.3901385084301092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58180.2992913821263681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.21862983396993196No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40290.20726108260349552No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27970.14388415190915288No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24050.12371876487004385No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23320.11996347595714855No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23230.11950049513227104No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22680.11667116786913076No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22620.1163625139858791No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20580.10586828195532234No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20320.10453078179456513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT11200.029.6875024
TAAGGTG12350.028.0769235
AGGTAAG10850.027.5806472
GGTAAGG11650.026.9098723
GTATCAA126800.025.7440191
AAGGTAA12550.024.231211
GTACATG71600.022.1652341
GGTGTGC17100.021.9467038
ATCAACG148500.021.942763
TCAACGC148800.021.8985214
CAACGCA149050.021.893665
AGAGTAC113550.021.5455910-11
TACATGG72500.021.4896552
TATCAAC151750.021.4415152
GGTATCA93550.021.4342121
AACGCAG153000.021.3273336
GAGTACT78950.020.87928612-13
AGTACTT82000.020.7978712-13
ACATGGG71900.020.4798323
TATAGGG5350.020.420562
Produced by FastQC (version 0.11.5)