FastQCFastQC Report
Thu 26 May 2016
SRR937221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094225
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66110.31567763731213216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47940.22891523117143575No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40220.19205195239288997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33100.15805369528107055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30640.1463071064474925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23340.11144934283565519No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23110.11035108453007676No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23070.11016008308562834No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22450.10719956069667777No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22010.10509854480774512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG10600.028.6943172
TAAGGTG13500.027.4595245
GTAAGGT11950.025.8510194
GTATCAA128300.025.7559451
GTACATG81250.025.046081
TACATGG80100.024.6227972
GGTAAGG13050.024.3997883
GGTATCA93150.023.1224941
GAGTACT71500.022.25415812-13
ACATGGG84900.022.0551683
AAGGTAA14350.021.8681161
TCAACGC150150.021.8401684
ATCAACG150350.021.8105933
CAACGCA151350.021.6356035
TATCAAC152300.021.500132
GTACTTT74050.021.4885814-15
AACGCAG153250.021.4175976
AGAGTAC114550.021.35451710-11
AGTACTT74500.021.23050712-13
CCCTATA4400.020.5221422
Produced by FastQC (version 0.11.5)