##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937221_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2094225 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7110348697012 33.0 31.0 34.0 30.0 34.0 2 31.805936802397067 33.0 31.0 34.0 30.0 34.0 3 31.863795914956608 33.0 31.0 34.0 30.0 34.0 4 35.50027384832098 37.0 35.0 37.0 33.0 37.0 5 35.476742947868544 37.0 35.0 37.0 33.0 37.0 6 35.54706872470723 37.0 35.0 37.0 33.0 37.0 7 35.482608124723946 37.0 35.0 37.0 33.0 37.0 8 35.46117728515322 37.0 35.0 37.0 33.0 37.0 9 37.07965380988194 39.0 37.0 39.0 33.0 39.0 10-11 36.986024663061514 39.0 37.0 39.0 33.0 39.0 12-13 36.879145984791506 39.0 37.0 39.0 33.0 39.0 14-15 38.05115352934857 40.0 38.0 41.0 33.0 41.0 16-17 37.99495971063281 40.0 38.0 41.0 33.0 41.0 18-19 37.93763611837315 40.0 38.0 41.0 33.0 41.0 20-21 37.84636392937722 40.0 38.0 41.0 33.0 41.0 22-23 37.7102233523141 40.0 37.5 41.0 32.0 41.0 24-25 37.55966980625291 40.0 37.0 41.0 32.0 41.0 26-27 37.3861182537693 40.0 37.0 41.0 32.0 41.0 28-29 37.2130310735475 39.0 37.0 41.0 31.5 41.0 30-31 37.18370327925605 39.0 37.0 41.0 31.0 41.0 32-33 37.03023863242966 39.0 36.0 41.0 31.0 41.0 34-35 36.82478864496413 39.0 36.0 41.0 30.5 41.0 36-37 36.85780539340329 39.0 36.0 41.0 30.5 41.0 38-39 36.8378746314269 39.0 36.0 41.0 31.0 41.0 40-41 36.68029032219556 39.0 36.0 41.0 30.0 41.0 42-43 36.44043834831501 39.0 35.0 41.0 30.0 41.0 44-45 36.22887703088254 39.0 35.0 41.0 30.0 41.0 46-47 35.92997481168452 39.0 35.0 41.0 28.5 41.0 48-49 35.617450846972034 39.0 35.0 40.0 27.5 41.0 50-51 34.66316202891284 38.0 34.0 39.5 26.0 40.5 52-53 34.77175637765761 38.0 34.0 39.5 26.5 40.5 54-55 35.1086817319056 38.0 35.0 40.0 26.5 41.0 56-57 34.85156251119149 38.0 34.0 40.0 26.0 41.0 58-59 34.577235254091605 38.0 34.0 40.0 26.0 41.0 60-61 34.26265683009228 37.5 34.0 40.0 25.0 41.0 62-63 33.92022562045625 37.0 33.5 40.0 24.5 41.0 64-65 33.53162196039108 36.0 33.0 39.0 23.0 41.0 66-67 33.11898363356373 36.0 33.0 39.0 22.5 41.0 68-69 32.61154770857955 35.0 32.0 39.0 21.5 40.0 70-71 32.1667323234132 35.0 32.0 38.0 20.0 40.0 72-73 31.761262519547806 35.0 31.0 37.0 18.5 39.5 74-75 31.319286132101375 35.0 31.0 37.0 18.0 39.0 76-77 30.900738459334598 35.0 31.0 36.0 15.0 39.0 78-79 30.452049564874834 34.0 30.0 36.0 9.5 37.5 80-81 30.039019685086366 34.0 30.0 35.0 7.0 37.0 82-83 29.6204808461364 34.0 29.5 35.0 4.5 36.5 84-85 29.187577027301266 34.0 29.0 35.0 2.0 36.0 86-87 28.794189258556266 34.0 29.0 35.0 2.0 36.0 88-89 28.409396077307832 34.0 28.5 35.0 2.0 35.0 90-91 27.998833697429838 33.5 27.0 35.0 2.0 35.0 92-93 27.580921581969463 33.0 27.0 35.0 2.0 35.0 94-95 27.044752115937875 33.0 25.5 35.0 2.0 35.0 96-97 26.409479162936172 33.0 25.0 35.0 2.0 35.0 98-99 25.69537227375282 32.0 23.5 34.5 2.0 35.0 100-101 23.835644212059357 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05321141618035696 1101 2 0.00165352225064197 1101 3 0.11834514127333762 1101 4 0.09902126409784984 1101 5 0.11982064368722689 1101 6 -0.13472705102152815 1101 7 -0.059810225025849206 1101 8 -0.005984083689348552 1101 9 -0.026049922994964447 1101 10-11 0.04699519797682683 1101 12-13 0.06197441843990248 1101 14-15 0.1434518696976852 1101 16-17 0.11644584253490109 1101 18-19 0.27254884176561234 1101 20-21 0.23696393397498383 1101 22-23 -0.013718066874830015 1101 24-25 0.2533180889075055 1101 26-27 0.24241287767090114 1101 28-29 0.09064181428215079 1101 30-31 -0.11256879139433806 1101 32-33 -0.08101908282615256 1101 34-35 -0.19431987033294718 1101 36-37 -0.04749569656190289 1101 38-39 0.14691106454656477 1101 40-41 0.15463066781574497 1101 42-43 0.21765545468775827 1101 44-45 0.06693232605713462 1101 46-47 0.13282137999593147 1101 48-49 0.2263960270256149 1101 50-51 0.16054787169723284 1101 52-53 0.23467825510508789 1101 54-55 0.14220179618694573 1101 56-57 0.3004477774751706 1101 58-59 -0.054468766992485484 1101 60-61 -0.045285411874374404 1101 62-63 0.18464379671655706 1101 64-65 0.06218666243208304 1101 66-67 0.32469278237815047 1101 68-69 0.3430412906399809 1101 70-71 0.25138492806993895 1101 72-73 0.20662088894484754 1101 74-75 0.1761351277911949 1101 76-77 0.06265711581783862 1101 78-79 0.340068966478821 1101 80-81 0.4791303547739467 1101 82-83 0.35963586062928954 1101 84-85 0.3094870571435635 1101 86-87 0.36755236525251433 1101 88-89 0.3873970071909163 1101 90-91 0.4235769326022272 1101 92-93 0.3732737095661882 1101 94-95 0.27470491075459336 1101 96-97 0.18787668601902396 1101 98-99 0.444373619624141 1101 100-101 0.7798211380455058 1102 1 -0.006336962634378551 1102 2 -0.028643940153052938 1102 3 0.09377308777200355 1102 4 -0.13683370137935924 1102 5 0.025063706859462798 1102 6 0.050674875540039466 1102 7 -0.032934183329061284 1102 8 -0.007275205805306939 1102 9 0.1608235160027789 1102 10-11 -0.0123751710351101 1102 12-13 0.14846346559107104 1102 14-15 0.22106149869668457 1102 16-17 0.2297287594481361 1102 18-19 0.3552079890536888 1102 20-21 0.31193180580651614 1102 22-23 0.3629890286188342 1102 24-25 0.3260145062816875 1102 26-27 0.46414158826275553 1102 28-29 0.4565757459306141 1102 30-31 0.2803479525656769 1102 32-33 0.3633178072941021 1102 34-35 0.4319262864539297 1102 36-37 0.42506019975051856 1102 38-39 0.5211036283038766 1102 40-41 0.49556819669053453 1102 42-43 0.3725908139413434 1102 44-45 0.3522532973928776 1102 46-47 0.42670269198196564 1102 48-49 0.4249076393659337 1102 50-51 0.4657217411630583 1102 52-53 0.3026667143566186 1102 54-55 0.36852287755549895 1102 56-57 0.3486074964400956 1102 58-59 0.2979089996095965 1102 60-61 0.30389479082580095 1102 62-63 0.3242219972428799 1102 64-65 0.49728000340187606 1102 66-67 0.5020001561983847 1102 68-69 0.4791898708993827 1102 70-71 0.31975911931118617 1102 72-73 0.29566299694768716 1102 74-75 0.2472682505574788 1102 76-77 0.24369299709244174 1102 78-79 0.30616475567853385 1102 80-81 0.29691761621965185 1102 82-83 0.37324942259574456 1102 84-85 0.4152044251870777 1102 86-87 0.4861086572598303 1102 88-89 0.6295160838464007 1102 90-91 0.599236723864351 1102 92-93 0.37385993402323336 1102 94-95 0.06857255680727192 1102 96-97 0.4605682189241236 1102 98-99 0.6392140332628422 1102 100-101 0.8755547041875644 1103 1 0.08142485403497801 1103 2 -0.032633823108209725 1103 3 -0.018730792143074382 1103 4 -0.07491965723966842 1103 5 -0.15246613079602156 1103 6 -0.1147634617456319 1103 7 -0.04653632309113931 1103 8 -0.00506023988648252 1103 9 0.08088343075993976 1103 10-11 0.08121866792579624 1103 12-13 0.14980693607090956 1103 14-15 0.18607051783641282 1103 16-17 0.2305302833900953 1103 18-19 0.15643448563670148 1103 20-21 0.10296011189323906 1103 22-23 0.11265690788828664 1103 24-25 0.2964317279609645 1103 26-27 0.05765055685274234 1103 28-29 -0.08238622824111275 1103 30-31 0.07763547067933985 1103 32-33 0.1465185288252755 1103 34-35 0.25791542091803166 1103 36-37 0.2152670280463198 1103 38-39 0.10993834126110613 1103 40-41 0.20949633800280765 1103 42-43 0.12995573525223136 1103 44-45 0.14034014009092033 1103 46-47 0.12839761625488677 1103 48-49 0.1309245673245556 1103 50-51 -0.20317437643871727 1103 52-53 -0.01057616744031975 1103 54-55 0.1352002158361998 1103 56-57 0.10788639094365493 1103 58-59 0.10178997889129704 1103 60-61 0.11025643261076112 1103 62-63 0.18849511843280453 1103 64-65 0.3742916617707053 1103 66-67 0.3476519021158282 1103 68-69 0.3122225366804017 1103 70-71 0.3666859605193409 1103 72-73 0.4376967109709007 1103 74-75 0.4963651836952323 1103 76-77 0.5696110071704616 1103 78-79 0.7221915097992273 1103 80-81 0.5838466922588914 1103 82-83 0.6050249958919984 1103 84-85 0.6121548562788988 1103 86-87 0.8482212643676554 1103 88-89 0.7376083007810976 1103 90-91 0.6761416956540458 1103 92-93 0.7012826416807236 1103 94-95 0.6404071225494405 1103 96-97 0.736527644324223 1103 98-99 0.8826609232116063 1103 100-101 1.1636258498577554 1104 1 0.04431420782391271 1104 2 0.05851652274924746 1104 3 0.03239271630260987 1104 4 -0.009327895832008437 1104 5 0.07582620680593521 1104 6 0.10198421023216042 1104 7 0.02118693459027554 1104 8 0.07710344719352236 1104 9 0.10406010686548228 1104 10-11 0.10385049526473722 1104 12-13 0.2047940456605275 1104 14-15 0.2077267594717398 1104 16-17 0.10606016378399374 1104 18-19 0.1603743374322093 1104 20-21 0.1895993424132243 1104 22-23 0.2297888267984476 1104 24-25 0.24788172303021128 1104 26-27 0.2337159527926147 1104 28-29 0.22613253681530665 1104 30-31 0.07753069207037555 1104 32-33 0.1608284692335431 1104 34-35 0.1809682997763531 1104 36-37 0.09055389250682566 1104 38-39 0.20236997982244986 1104 40-41 0.07624594706413035 1104 42-43 0.1801114063284075 1104 44-45 0.2634345781958203 1104 46-47 0.3173139765101567 1104 48-49 0.46004351129705867 1104 50-51 0.3242295442183405 1104 52-53 0.28631287641525205 1104 54-55 0.3368703340335131 1104 56-57 0.2726938096213729 1104 58-59 0.28935143726865675 1104 60-61 0.4223029789669326 1104 62-63 0.5511881223915012 1104 64-65 0.35274301632038174 1104 66-67 0.46221155020058546 1104 68-69 0.4064614741724739 1104 70-71 0.3575040085026586 1104 72-73 0.5006202278693088 1104 74-75 0.5289127655105936 1104 76-77 0.45438253697337316 1104 78-79 0.45498721440455725 1104 80-81 0.5647970606569608 1104 82-83 0.6464456529507281 1104 84-85 0.618305272575757 1104 86-87 0.6607330629673207 1104 88-89 0.5326032291442253 1104 90-91 0.5876171545667432 1104 92-93 0.8050425208067118 1104 94-95 0.8412270371768855 1104 96-97 1.0454320651010285 1104 98-99 1.0967361243160916 1104 100-101 0.9692514926172926 1105 1 0.1413109000506445 1105 2 0.14267281275804322 1105 3 0.17735838562552075 1105 4 0.1597398679583506 1105 5 0.19604565432579335 1105 6 0.1383036518290055 1105 7 0.11090003309904262 1105 8 0.1793659231028215 1105 9 0.1244341221650771 1105 10-11 0.18385953924536125 1105 12-13 0.24040044797870053 1105 14-15 0.42536605271996564 1105 16-17 0.2526743092986834 1105 18-19 0.3229390349258594 1105 20-21 0.17686572523103194 1105 22-23 0.25731316809140026 1105 24-25 0.2806000884617035 1105 26-27 0.3066623768892427 1105 28-29 0.40229452886024575 1105 30-31 0.2403209195595224 1105 32-33 0.20074471559431117 1105 34-35 0.4053327721956279 1105 36-37 0.3746012333031459 1105 38-39 0.34873723833450043 1105 40-41 0.4122540319727577 1105 42-43 0.44935093740826204 1105 44-45 0.40996842136362943 1105 46-47 0.32318117351402265 1105 48-49 0.2704046782211478 1105 50-51 0.2613473961704287 1105 52-53 0.25755153726825597 1105 54-55 0.3843979329819547 1105 56-57 0.4675989959000333 1105 58-59 0.4409149980000038 1105 60-61 0.5485291559373522 1105 62-63 0.5436479598276591 1105 64-65 0.566587311778342 1105 66-67 0.6125856680138 1105 68-69 0.657822342667977 1105 70-71 0.7138177662880025 1105 72-73 0.7265067429980441 1105 74-75 0.4080417868926638 1105 76-77 0.590200857754482 1105 78-79 0.7749883648353411 1105 80-81 0.7679335222507966 1105 82-83 0.8182274652095352 1105 84-85 0.7873793010882153 1105 86-87 0.724271583034934 1105 88-89 0.8802423401459869 1105 90-91 0.7683404338184374 1105 92-93 0.9876184492385285 1105 94-95 0.9156382672038106 1105 96-97 0.7638826398608032 1105 98-99 0.8583418067607447 1105 100-101 1.1870103851055482 1106 1 0.030998593898541316 1106 2 0.14506288063707018 1106 3 -0.08234913624411888 1106 4 0.0032857326352768723 1106 5 0.04336036641326757 1106 6 0.07727811235533721 1106 7 0.12697274769315925 1106 8 0.04402169155079605 1106 9 0.09734978917956028 1106 10-11 -0.029593071105651347 1106 12-13 -0.09679122543693808 1106 14-15 -0.09701547209976269 1106 16-17 -0.20387668121382774 1106 18-19 -0.185794116967962 1106 20-21 -0.1754658656397865 1106 22-23 -0.12850486686109974 1106 24-25 -0.18710289488124232 1106 26-27 -0.06830440715457797 1106 28-29 0.10775954801548693 1106 30-31 0.11089632147258754 1106 32-33 0.10986977345777404 1106 34-35 -0.03199347873420777 1106 36-37 -0.21679373953593029 1106 38-39 -0.20102608211018946 1106 40-41 0.041694285988079116 1106 42-43 -0.032506486167534376 1106 44-45 -0.011902043177393296 1106 46-47 0.014428176673142445 1106 48-49 0.11794365090452175 1106 50-51 0.056401561451330906 1106 52-53 0.08022921301451191 1106 54-55 0.18406097085830453 1106 56-57 0.1718526904875688 1106 58-59 0.07896347383191227 1106 60-61 0.15317486227784372 1106 62-63 0.29542734071608123 1106 64-65 0.20213633979907542 1106 66-67 0.2781993677127872 1106 68-69 0.2184213638762671 1106 70-71 0.22863282322903444 1106 72-73 0.3631060934039603 1106 74-75 0.30261444774886215 1106 76-77 0.2552859583036238 1106 78-79 0.2875771064456707 1106 80-81 0.23680244912576498 1106 82-83 0.09085969645013847 1106 84-85 0.17002320234246682 1106 86-87 0.12399292866620826 1106 88-89 0.30596299945521466 1106 90-91 0.4451295044571424 1106 92-93 0.38031091772144876 1106 94-95 0.4000966905587333 1106 96-97 0.3331130494283556 1106 98-99 0.44101695640180694 1106 100-101 0.8994193374192818 1107 1 -0.034316588326568365 1107 2 -0.13388319622527334 1107 3 -0.10219462694615089 1107 4 -0.03667485624983158 1107 5 0.06269290528680926 1107 6 0.040755121444021825 1107 7 -0.08507251025184814 1107 8 0.030028365669622303 1107 9 -0.07731400167397595 1107 10-11 0.005209889734373974 1107 12-13 0.04924246688059952 1107 14-15 0.14646121690698521 1107 16-17 0.09645127966723521 1107 18-19 -0.022963304074728796 1107 20-21 0.1427645244169824 1107 22-23 -0.029520142156187035 1107 24-25 0.053215241946404035 1107 26-27 0.009671041739828468 1107 28-29 -0.1073449546346339 1107 30-31 0.045767853713044815 1107 32-33 -0.18928188629019616 1107 34-35 -0.11128659828563059 1107 36-37 -0.11135197721400658 1107 38-39 -0.17189431668593613 1107 40-41 -0.09173802653007357 1107 42-43 0.0220213191044607 1107 44-45 -0.12202931268145534 1107 46-47 -0.1631600551449779 1107 48-49 0.018926302463313505 1107 50-51 -0.08848188768600806 1107 52-53 0.037807481256308506 1107 54-55 0.09903263950290864 1107 56-57 0.0584417085582416 1107 58-59 0.08001728206835423 1107 60-61 0.02379692101205677 1107 62-63 0.03479220786565662 1107 64-65 0.06049474621778472 1107 66-67 0.025384288078036832 1107 68-69 0.07599639026251026 1107 70-71 0.21298963908671453 1107 72-73 0.10060299517491345 1107 74-75 0.08353046481333237 1107 76-77 0.0247598667010962 1107 78-79 0.06342228691443097 1107 80-81 0.25444286250748505 1107 82-83 0.23176571710937566 1107 84-85 0.2961566207026962 1107 86-87 0.2793834778775768 1107 88-89 0.2449332219860274 1107 90-91 0.35600145690773743 1107 92-93 0.36240253830452573 1107 94-95 0.22331563973792612 1107 96-97 0.40817862519010717 1107 98-99 0.5840369541366073 1107 100-101 0.7635295752812006 1108 1 -0.06649276703415552 1108 2 -0.17959144254849946 1108 3 -0.1559014172469979 1108 4 -0.16929742076503373 1108 5 -0.02027163290504319 1108 6 0.03303437120933239 1108 7 -0.0015587706371817944 1108 8 -0.058900755416516404 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-1.3286250589715678 2316 84-85 -1.5105182653976357 2316 86-87 -1.7376016288111735 2316 88-89 -1.6727783969146337 2316 90-91 -1.8198020179460883 2316 92-93 -1.802446272020699 2316 94-95 -1.8836761736052878 2316 96-97 -2.001872027700184 2316 98-99 -2.1788477645242814 2316 100-101 -2.132789328659843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7275.0 3 1386.0 4 1276.0 5 1416.0 6 1671.0 7 1998.0 8 2612.0 9 3179.0 10 4000.0 11 5252.0 12 7035.0 13 8580.0 14 10003.0 15 12128.0 16 15262.0 17 19238.0 18 16504.0 19 12699.0 20 12459.0 21 13432.0 22 14409.0 23 16259.0 24 18561.0 25 21158.0 26 24225.0 27 29240.0 28 34981.0 29 42658.0 30 52401.0 31 65505.0 32 83807.0 33 109382.0 34 150370.0 35 215138.0 36 316556.0 37 415747.0 38 292624.0 39 33794.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66752323634857 18.386567582389947 14.878112449318296 23.067796731943183 2 15.398085001347884 19.665677261868908 39.6992809537741 25.236956783009116 3 19.334174800422904 23.628290532154512 29.519070913074792 27.518463754347795 4 12.967833716156976 15.956012869003446 36.47018883572293 34.60596457911665 5 14.086950110404024 37.135393825418014 33.757633964222585 15.02002209995538 6 32.964079449157616 35.66656618290915 16.666570967849985 14.702783400083257 7 29.222517156514407 32.54528932192708 19.956492007823016 18.275701513735502 8 27.258232766458356 34.0325004067336 19.222468920768296 19.486797906039754 9 26.532842646322074 16.668253695584248 19.856507065787692 36.94239659230599 10-11 25.365340796115728 26.139941047783644 26.70515301902201 21.78956513707862 12-13 26.4212996106566 23.344835935974643 27.29528844718214 22.93857600618662 14-15 23.116926862726196 24.883677923554345 25.43140663767297 26.567988576046492 16-17 22.54487031483092 28.180938355730113 26.212303013844807 23.06188831559416 18-19 22.757905303343843 26.684277335012425 28.44225010179324 22.1155672598505 20-21 23.2707371229508 25.58091074363992 28.641683846273285 22.506668287136 22-23 23.057121195483013 25.327839378621377 28.810462151068446 22.804577274827164 24-25 23.13626265267354 25.694678515100676 28.58448659563571 22.584572236590073 26-27 22.439311956544625 25.93443700750478 28.700377248706616 22.92587378724398 28-29 22.576930622478912 26.527032781943376 28.401958016527345 22.494078579050374 30-31 22.622937405071106 25.945867440740923 28.224735156159525 23.206459998028446 32-33 22.386753370923895 26.05795830902772 28.273099268845563 23.282189051202824 34-35 23.267440077266098 26.030057124182477 28.042071456785077 22.660431341766348 36-37 23.28445527504554 25.56973496659989 28.173345652530692 22.972464105823885 38-39 22.712231263918085 26.06022635417915 28.16925633626401 23.058286045638752 40-41 22.976454898302144 25.94903445347495 28.210584231898615 22.863926416324283 42-43 22.789720114661105 25.988112390465893 27.794984067005412 23.427183427867586 44-45 22.82603910564803 26.02240569310555 27.97110859947192 23.180446601774502 46-47 23.524179647924136 26.447005010687636 27.245581109384666 22.78323423200357 48-49 23.168619325943773 26.624383638334166 27.007203745660647 23.199793290061415 50-51 23.087171050270396 26.754960834447118 27.301062965882018 22.85680514940047 52-53 23.51241572798459 26.506571273038336 26.95386051074674 23.027152488230332 54-55 23.089435446416328 26.642059374782406 27.017916376841868 23.250588801959395 56-57 23.214659896262404 26.803615219822856 26.842174757950392 23.139550125964348 58-59 23.25699592183099 26.392433658300096 26.922205774970504 23.428364644898416 60-61 22.751390334038966 26.71264037888845 27.5362922033655 22.99967708370708 62-63 22.827559581729513 26.75714554982438 27.538628019989265 22.876666848456843 64-65 22.62126354714562 26.925542424003236 27.217163345508123 23.23603068334302 66-67 22.66468305656805 26.825240649079714 27.014606996651164 23.495469297701074 68-69 22.99868251602126 26.465952080706177 27.364821551244102 23.170543852028466 70-71 23.040671702039862 26.5596440553293 26.97852491074472 23.421159331886113 72-73 22.91633273221144 26.663639525909566 27.015867608365816 23.404160133513173 74-75 22.748533325359567 27.390772358939092 26.74841371886493 23.112280596836406 76-77 23.10346700210454 26.734846743152946 26.844559425589754 23.31712682915276 78-79 23.029971735159762 26.930782839187057 26.882673999815793 23.156571425837388 80-81 23.303753946190678 26.98285467158652 26.603269280541085 23.110122101681714 82-83 23.145773565622793 26.904608341674496 26.87432178201715 23.07529631068556 84-85 22.91041888015109 27.249334698936618 26.682004987545486 23.158241433366804 86-87 23.322234421457257 27.180804623270877 26.43990441540548 23.057056539866384 88-89 23.021593920240598 27.05786215668408 26.212276304769734 23.70826761830559 90-91 22.86109119022798 26.9495261966125 26.603556671018698 23.585825942140826 92-93 23.14554767812493 26.711849988373636 26.89894826891628 23.24365406458515 94-95 22.832363650542923 26.45610902550169 27.13930060836322 23.57222671559217 96-97 22.870316859497173 26.28091475526536 27.24698889709625 23.60177948814121 98-99 22.666121242132608 26.850484303495264 26.634993768285476 23.848400686086652 100-101 22.70672336020432 26.296049048504834 27.232367841781112 23.76485974950973 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2738.0 1 1548.5 2 362.5 3 341.0 4 297.0 5 285.5 6 434.0 7 875.0 8 1426.5 9 1766.0 10 1688.5 11 1578.5 12 1733.5 13 1860.0 14 1994.0 15 2120.5 16 2210.5 17 2344.5 18 2523.0 19 2806.5 20 3187.5 21 3714.5 22 4380.5 23 5241.5 24 6301.5 25 7853.5 26 9988.0 27 12493.5 28 14755.0 29 16962.0 30 19958.0 31 24264.0 32 30830.5 33 33792.5 34 33517.0 35 35511.5 36 39360.5 37 44242.5 38 48707.0 39 51749.0 40 54768.0 41 59169.5 42 61383.0 43 63134.0 44 66715.5 45 71249.0 46 74615.5 47 79959.5 48 88062.5 49 87338.5 50 80728.0 51 77183.0 52 76978.5 53 79136.0 54 80877.0 55 76613.0 56 69759.0 57 64111.5 58 59653.5 59 53553.5 60 43916.0 61 36564.0 62 30296.0 63 22265.0 64 15475.0 65 11454.5 66 8330.5 67 6354.0 68 4942.5 69 3749.0 70 2839.5 71 1953.0 72 1335.5 73 991.5 74 731.5 75 516.0 76 368.5 77 248.5 78 161.5 79 111.5 80 74.0 81 48.0 82 28.5 83 17.5 84 13.0 85 12.5 86 8.5 87 6.0 88 9.0 89 7.5 90 6.5 91 7.0 92 5.0 93 4.0 94 4.0 95 3.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30751232556196206 2 0.27523308145017844 3 0.2773818477002232 4 0.27676109300576585 5 0.264202748033282 6 0.20699781542097914 7 0.21459012283780396 8 0.21034034069882654 9 0.2084780766154544 10-11 0.21805202401843166 12-13 0.21740739414341823 14-15 0.20200790268476407 16-17 0.21260848285165157 18-19 0.20074251811529326 20-21 0.20511167615705092 22-23 0.2049206747126025 24-25 0.1959436068235266 26-27 0.20804832336544543 28-29 0.20795282264322126 30-31 0.2150198760878129 32-33 0.2156406307822703 34-35 0.20554142940705988 36-37 0.20351203905979537 38-39 0.19546610321240554 40-41 0.1951796010457329 42-43 0.19412909310126658 44-45 0.2100538385321539 46-47 0.20019338896250402 48-49 0.20723656722653966 50-51 0.21516312717114924 52-53 0.22144229965739118 54-55 0.21967553629624326 56-57 0.18866167675393045 58-59 0.17684346237868423 60-61 0.18622640833721305 62-63 0.18636965942054937 64-65 0.18336138667048668 66-67 0.19560935429574186 68-69 0.1851281500316346 70-71 0.19159832396232496 72-73 0.20265253255977747 74-75 0.1927920829901276 76-77 0.201530399073643 78-79 0.1992383817402619 80-81 0.18708591483723094 82-83 0.20002626269861168 84-85 0.18231087872602036 86-87 0.18765891917057623 88-89 0.19465434707349974 90-91 0.1915744487817689 92-93 0.19802074753190324 94-95 0.19618235862908714 96-97 0.1891391803650515 98-99 0.19625398417075526 100-101 0.18166624885100693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2094225.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.576462182529035 #Duplication Level Percentage of deduplicated Percentage of total 1 69.74551811311653 31.787539686833494 2 14.70296857033197 13.402185820332962 3 5.944395440814019 8.127735420187745 4 2.9564811173500476 5.389837993530625 5 1.7561951725350693 4.002058143309233 6 1.1050798091397875 3.0219376877961563 7 0.7230465591831431 2.3067732912572785 8 0.5248145983997352 1.9135354157443754 9 0.3798056263459354 1.557917708928055 >10 1.982744718946889 15.91829470905435 >50 0.11676253075150589 3.6162339852670256 >100 0.05424736957758092 4.409417005038977 >500 0.004388101149048336 1.3992446177730213 >1k 0.003447793759966544 2.8324882369784 >5k 1.0447859878686497E-4 0.31480027796826376 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6611 0.31567763731213216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4794 0.22891523117143575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4022 0.19205195239288997 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3310 0.15805369528107055 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3064 0.1463071064474925 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2334 0.11144934283565519 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2311 0.11035108453007676 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2307 0.11016008308562834 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2245 0.10719956069667777 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2201 0.10509854480774512 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.775036111210591E-5 2 0.0 0.0 0.0 0.0 4.775036111210591E-5 3 0.0 0.0 0.0 0.0 4.775036111210591E-5 4 0.0 0.0 0.0 0.0 4.775036111210591E-5 5 0.0 0.0 0.0 4.775036111210591E-5 4.775036111210591E-5 6 0.0 0.0 0.0 4.775036111210591E-5 4.775036111210591E-5 7 0.0 0.0 0.0 4.775036111210591E-5 4.775036111210591E-5 8 0.0 0.0 0.0 4.775036111210591E-5 4.775036111210591E-5 9 0.0 0.0 0.0 4.775036111210591E-5 4.775036111210591E-5 10-11 0.0 0.0 0.0 4.775036111210591E-5 9.550072222421182E-5 12-13 2.3875180556052956E-5 0.0 0.0 4.775036111210591E-5 9.550072222421182E-5 14-15 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 1.6712626389237068E-4 16-17 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 2.3875180556052954E-4 18-19 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 2.626269861165825E-4 20-21 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 2.8650216667263544E-4 22-23 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 2.8650216667263544E-4 24-25 1.4325108333631772E-4 0.0 0.0 1.1937590278026477E-4 2.8650216667263544E-4 26-27 1.4325108333631772E-4 0.0 0.0 2.1487662500447658E-4 3.581277083407943E-4 28-29 1.9100144444842364E-4 0.0 0.0 5.49129152789218E-4 3.820028888968473E-4 30-31 1.9100144444842364E-4 0.0 0.0 0.0014802611944752832 3.820028888968473E-4 32-33 1.9100144444842364E-4 0.0 0.0 0.002530769138941613 3.820028888968473E-4 34-35 2.1487662500447658E-4 0.0 0.0 0.003223149375067149 3.820028888968473E-4 36-37 2.3875180556052954E-4 0.0 0.0 0.003939404791748738 3.820028888968473E-4 38-39 2.3875180556052954E-4 0.0 0.0 0.00565841779178455 4.058780694529003E-4 40-41 2.3875180556052954E-4 0.0 0.0 0.008595065000179064 4.297532500089532E-4 42-43 2.3875180556052954E-4 0.0 0.0 0.012224092444699113 4.297532500089532E-4 44-45 2.3875180556052954E-4 0.0 0.0 0.01972089913929974 4.297532500089532E-4 46-47 2.8650216667263544E-4 0.0 0.0 0.03703040504243813 4.297532500089532E-4 48-49 2.8650216667263544E-4 0.0 0.0 0.0513555133760699 4.775036111210591E-4 50-51 2.8650216667263544E-4 0.0 0.0 0.0701930308347957 4.775036111210591E-4 52-53 2.8650216667263544E-4 0.0 0.0 0.09452183982141366 4.775036111210591E-4 54-55 2.8650216667263544E-4 0.0 0.0 0.12560732490539459 4.775036111210591E-4 56-57 2.8650216667263544E-4 0.0 0.0 0.15922357912831717 4.775036111210591E-4 58-59 2.8650216667263544E-4 0.0 0.0 0.2032971624347909 4.775036111210591E-4 60-61 2.8650216667263544E-4 0.0 0.0 0.26291348828325517 5.730043333452709E-4 62-63 2.8650216667263544E-4 0.0 0.0 0.3383113084792704 5.730043333452709E-4 64-65 2.8650216667263544E-4 0.0 0.0 0.39993314949444303 5.730043333452709E-4 66-67 2.8650216667263544E-4 0.0 0.0 0.5049361935799639 5.730043333452709E-4 68-69 2.8650216667263544E-4 0.0 0.0 0.6348410509854481 5.730043333452709E-4 70-71 2.8650216667263544E-4 0.0 0.0 0.7299358951402071 5.730043333452709E-4 72-73 2.8650216667263544E-4 0.0 0.0 0.8957490241019948 6.207546944573769E-4 74-75 2.8650216667263544E-4 0.0 0.0 1.038809105993864 7.401305972376416E-4 76-77 2.8650216667263544E-4 0.0 0.0 1.1711969821771777 7.640057777936946E-4 78-79 2.8650216667263544E-4 0.0 0.0 1.3412598932779427 7.640057777936946E-4 80-81 2.8650216667263544E-4 0.0 0.0 1.5151428332676766 7.878809583497476E-4 82-83 3.1037734722868843E-4 0.0 0.0 1.731738471272189 8.117561389058004E-4 84-85 3.3425252778474136E-4 0.0 0.0 2.0354068927646267 8.117561389058004E-4 86-87 3.3425252778474136E-4 0.0 0.0 2.414329883369743 8.595065000179063E-4 88-89 3.3425252778474136E-4 0.0 0.0 2.8592438730317897 9.072568611300123E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 1060 0.0 28.694317 2 TAAGGTG 1350 0.0 27.459524 5 GTAAGGT 1195 0.0 25.851019 4 GTATCAA 12830 0.0 25.755945 1 GTACATG 8125 0.0 25.04608 1 TACATGG 8010 0.0 24.622797 2 GGTAAGG 1305 0.0 24.399788 3 GGTATCA 9315 0.0 23.122494 1 GAGTACT 7150 0.0 22.254158 12-13 ACATGGG 8490 0.0 22.055168 3 AAGGTAA 1435 0.0 21.868116 1 TCAACGC 15015 0.0 21.840168 4 ATCAACG 15035 0.0 21.810593 3 CAACGCA 15135 0.0 21.635603 5 TATCAAC 15230 0.0 21.50013 2 GTACTTT 7405 0.0 21.48858 14-15 AACGCAG 15325 0.0 21.417597 6 AGAGTAC 11455 0.0 21.354517 10-11 AGTACTT 7450 0.0 21.230507 12-13 CCCTATA 440 0.0 20.522142 2 >>END_MODULE