FastQCFastQC Report
Thu 26 May 2016
SRR937221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094225
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81200.3877329322303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63760.3044563024507873No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44140.21077009394883547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43980.21000608817104177No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28950.1382372954195466No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25100.11985340639138584No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24580.11737038761355634No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24470.11684513364132318No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24370.1163676300302021No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24020.1146963673912784No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22100.10552829805775407No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20980.1001802576131982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA124100.027.4921381
TAAGGTG12050.025.6246345
AGGTAAG10650.024.9786992
GTAAGGT11650.024.8734074
GTACATG75300.024.1690521
GGTATCA89800.024.1292781
TACATGG76350.023.3943062
TCAACGC149850.022.6663024
ATCAACG150000.022.6119653
CAACGCA151000.022.5251375
TATCAAC152400.022.2558732
GAGTACT82900.022.23141512-13
GGTAAGG13350.022.0618463
AGAGTAC120600.022.03650310-11
AACGCAG155350.021.9223656
ACATGGG76700.021.6772443
AGTACTT86000.021.34720212-13
GTACTTT84800.021.14465714-15
ACCGAGT6200.020.6853088
CCCTATA4000.020.1892572
Produced by FastQC (version 0.11.5)