##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937221_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2094225 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22610607742721 33.0 31.0 34.0 31.0 34.0 2 32.45276128400721 34.0 31.0 34.0 31.0 34.0 3 32.539123542121786 34.0 31.0 34.0 31.0 34.0 4 36.12800296052239 37.0 35.0 37.0 35.0 37.0 5 36.0249548162208 37.0 35.0 37.0 35.0 37.0 6 36.10136207905072 37.0 35.0 37.0 35.0 37.0 7 36.056540725089235 37.0 35.0 37.0 35.0 37.0 8 36.03190822380594 37.0 35.0 37.0 35.0 37.0 9 37.74946531533145 39.0 37.0 39.0 35.0 39.0 10-11 37.67220475354845 39.0 37.0 39.0 35.0 39.0 12-13 37.605079683415106 39.0 37.0 39.0 35.0 39.0 14-15 38.857853860019816 40.0 38.0 41.0 35.0 41.0 16-17 38.82874595614129 40.0 38.0 41.0 35.0 41.0 18-19 38.81994842961 40.0 38.0 41.0 35.0 41.0 20-21 38.69481598204587 40.0 38.0 41.0 34.0 41.0 22-23 38.57573183397199 40.0 38.0 41.0 34.0 41.0 24-25 38.42743807375044 40.0 38.0 41.0 34.0 41.0 26-27 38.36882378922991 40.0 38.0 41.0 34.0 41.0 28-29 38.2858262603111 40.0 38.0 41.0 34.0 41.0 30-31 38.376993876016186 40.0 38.0 41.0 34.0 41.0 32-33 38.314096622855715 40.0 38.0 41.0 34.0 41.0 34-35 38.21060058016689 40.0 38.0 41.0 34.0 41.0 36-37 38.05760173811315 40.0 37.5 41.0 33.5 41.0 38-39 37.91792715682412 40.0 37.0 41.0 33.0 41.0 40-41 37.76254390048825 40.0 37.0 41.0 33.0 41.0 42-43 37.56263486492617 40.0 37.0 41.0 33.0 41.0 44-45 37.29135861714954 40.0 37.0 41.0 32.0 41.0 46-47 36.96646157886569 40.0 36.0 41.0 31.5 41.0 48-49 36.661800188613924 39.0 36.0 41.0 31.0 41.0 50-51 36.4371361720923 39.0 36.0 41.0 30.5 41.0 52-53 36.19852379758622 39.0 35.0 41.0 30.0 41.0 54-55 35.95116331817259 39.0 35.0 40.0 29.5 41.0 56-57 35.66589860210818 38.5 35.0 40.0 29.0 41.0 58-59 35.38569566307345 38.0 35.0 40.0 28.0 41.0 60-61 35.031833494490805 38.0 34.0 40.0 28.0 41.0 62-63 34.64693645620695 37.0 34.0 40.0 27.5 41.0 64-65 34.26718237056668 36.5 34.0 39.0 26.5 41.0 66-67 33.81993410450167 36.0 33.0 39.0 26.0 40.5 68-69 33.40949659181798 35.5 33.0 39.0 26.0 40.0 70-71 32.98687390323389 35.0 33.0 38.0 25.5 40.0 72-73 32.54434432785398 35.0 32.0 37.0 24.5 39.0 74-75 32.124098890997864 35.0 32.0 37.0 24.0 39.0 76-77 30.81514641454476 33.5 30.5 35.0 22.0 37.5 78-79 31.464165502751612 34.0 31.0 36.0 23.0 37.5 80-81 31.34923659110172 34.5 31.0 35.0 23.0 37.0 82-83 31.051419976363572 34.0 31.0 35.0 21.0 37.0 84-85 30.720932325800714 34.0 31.0 35.0 20.0 36.0 86-87 30.397868662631762 34.0 31.0 35.0 18.5 36.0 88-89 30.020917284436962 34.0 30.5 35.0 17.5 35.5 90-91 29.615429574186155 34.0 30.0 35.0 10.0 35.0 92-93 29.291603576502048 34.0 29.5 35.0 4.5 35.0 94-95 28.899742625553607 34.0 29.0 35.0 2.0 35.0 96-97 28.44629254258735 34.0 29.0 35.0 2.0 35.0 98-99 27.966579999761247 33.5 29.0 35.0 2.0 35.0 100-101 26.03690601535174 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1561046618561832 1101 2 0.13675280276993362 1101 3 0.07628249956696465 1101 4 0.05828451955429159 1101 5 -0.053488622714262135 1101 6 -0.007833339737956635 1101 7 0.053311717382833024 1101 8 0.039422319110911985 1101 9 0.09721847526068927 1101 10-11 0.06475698535827945 1101 12-13 0.024730712677573763 1101 14-15 0.008666537022556042 1101 16-17 0.04724454346154516 1101 18-19 -0.08551760512114726 1101 20-21 0.01223568398953745 1101 22-23 -0.05147625960913871 1101 24-25 -0.06729604376543818 1101 26-27 0.02877472484341581 1101 28-29 -0.12539161730531134 1101 30-31 -0.03539389948358007 1101 32-33 0.06252560193568257 1101 34-35 0.004806892805127916 1101 36-37 -0.02757558539966709 1101 38-39 -0.059214940583245834 1101 40-41 -0.029470955062976145 1101 42-43 -0.06139447576857293 1101 44-45 -0.041795834335154325 1101 46-47 -0.004456206497174264 1101 48-49 -0.05276461916786701 1101 50-51 -0.1266657538804168 1101 52-53 -0.14402355613032825 1101 54-55 -0.16582050426867312 1101 56-57 -0.1232511244222323 1101 58-59 -0.20383616977277086 1101 60-61 -0.2201665960498147 1101 62-63 -0.25702466104203125 1101 64-65 -0.31480785883075413 1101 66-67 -0.15173207876896555 1101 68-69 -0.22739178259417514 1101 70-71 -0.40475832019509284 1101 72-73 -0.5386853719194598 1101 74-75 -0.5205387795747569 1101 76-77 -0.4260459444609275 1101 78-79 -0.36550404347835297 1101 80-81 -0.3490755067720279 1101 82-83 -0.3130828332797897 1101 84-85 -0.3083144739779655 1101 86-87 -0.3685605742133937 1101 88-89 -0.3252891414740553 1101 90-91 -0.24671248827063152 1101 92-93 -0.22472227582368376 1101 94-95 -0.20850141911809672 1101 96-97 -0.12819151787747884 1101 98-99 -0.14808740841023393 1101 100-101 0.05424838849225111 1102 1 0.20043906565823733 1102 2 0.18463082717323687 1102 3 0.1581253938811642 1102 4 0.12913771110518013 1102 5 0.021949974395681693 1102 6 0.07638407662123825 1102 7 0.10741241432779702 1102 8 0.2166377344248005 1102 9 0.21153056920613267 1102 10-11 0.24927441744653578 1102 12-13 0.16133150307988586 1102 14-15 0.14321576224349286 1102 16-17 0.0150849874298018 1102 18-19 0.09876406757499012 1102 20-21 0.12257019782439471 1102 22-23 0.16793832354639449 1102 24-25 0.13363223459499096 1102 26-27 0.1684352736390835 1102 28-29 0.0455288282711237 1102 30-31 0.13319601953651272 1102 32-33 0.05792176940843774 1102 34-35 0.12364505570869966 1102 36-37 0.11293251576561403 1102 38-39 0.0826551692167996 1102 40-41 0.24289409428603648 1102 42-43 0.25329176498179606 1102 44-45 0.15120770152672236 1102 46-47 0.1464351826599355 1102 48-49 0.0018596152753644901 1102 50-51 0.14137874451458998 1102 52-53 0.15270273867718487 1102 54-55 0.14225495143289635 1102 56-57 0.09812826727974766 1102 58-59 0.11745671216273479 1102 60-61 0.10106650844819853 1102 62-63 -0.03651327652604408 1102 64-65 0.12245748386853705 1102 66-67 0.07295103027848171 1102 68-69 0.01047445560286775 1102 70-71 0.012444198004395446 1102 72-73 0.06624325135148723 1102 74-75 -0.12244493922529998 1102 76-77 -0.12529287218277574 1102 78-79 -0.0761132690237396 1102 80-81 0.06797439381050907 1102 82-83 0.11187338283310311 1102 84-85 -0.011637200195892206 1102 86-87 -0.10365518479248337 1102 88-89 0.12164789084244276 1102 90-91 0.13280423495242744 1102 92-93 0.17902793842288034 1102 94-95 0.12003819868718679 1102 96-97 0.09425399296040027 1102 98-99 0.04817267528523317 1102 100-101 0.295276659164486 1103 1 0.15491626594699426 1103 2 0.18866558411762213 1103 3 0.12541261046999352 1103 4 0.1451185270313644 1103 5 0.03704075997428191 1103 6 0.07005010417351798 1103 7 0.04136127216948182 1103 8 0.13647221503054396 1103 9 0.1732507506176617 1103 10-11 0.11714073907419476 1103 12-13 0.12520377361506263 1103 14-15 0.1360502907384742 1103 16-17 0.1648261194959062 1103 18-19 0.1944739013638639 1103 20-21 0.12425714055773796 1103 22-23 0.15801593958467208 1103 24-25 0.08806757614280514 1103 26-27 0.1757522170286876 1103 28-29 0.03050162895789299 1103 30-31 0.12384227592839636 1103 32-33 0.19280411264656294 1103 34-35 0.20421878651087155 1103 36-37 0.3264590082691825 1103 38-39 0.2601239924363341 1103 40-41 0.249800343122061 1103 42-43 0.1866373957983427 1103 44-45 0.15828910081476266 1103 46-47 0.3070067318412981 1103 48-49 0.39565955532512476 1103 50-51 0.31531414015950077 1103 52-53 0.3993440181357144 1103 54-55 0.38166903548027875 1103 56-57 0.26673749287529347 1103 58-59 0.34658241431650794 1103 60-61 0.19670504048344384 1103 62-63 0.09564487856768977 1103 64-65 0.1862021384541066 1103 66-67 0.30700231787817955 1103 68-69 0.4533871145020143 1103 70-71 0.3572410323484334 1103 72-73 0.3806439884910162 1103 74-75 0.38557881383650994 1103 76-77 0.5438161751583976 1103 78-79 0.5736118999631898 1103 80-81 0.42608479091870777 1103 82-83 0.4044681104054213 1103 84-85 0.5592174985364622 1103 86-87 0.5459281788411374 1103 88-89 0.4382816853905176 1103 90-91 0.629818607044708 1103 92-93 0.5573303004264964 1103 94-95 0.6433149399487306 1103 96-97 0.5817572077471773 1103 98-99 0.8383950232413362 1103 100-101 0.9600382505770604 1104 1 0.06196302362136663 1104 2 0.042957809016542114 1104 3 0.04002068564292216 1104 4 0.02253812045514536 1104 5 -0.011093652490671957 1104 6 -0.0018220342258175037 1104 7 -0.02502293249924037 1104 8 -0.026697839771308907 1104 9 0.04058306962362224 1104 10-11 -0.060708358597075573 1104 12-13 0.06861170227403335 1104 14-15 0.007359442225443047 1104 16-17 -0.041332925627571626 1104 18-19 -0.10283267204103907 1104 20-21 -0.142872665170394 1104 22-23 -0.046689764598013994 1104 24-25 -0.107431522232865 1104 26-27 -0.16712072437982073 1104 28-29 -0.17329998551317516 1104 30-31 -0.05511236814130882 1104 32-33 -0.03691409452979855 1104 34-35 -0.007108573561254161 1104 36-37 -0.11099925638573183 1104 38-39 -0.17961052146136325 1104 40-41 -0.06991001006110054 1104 42-43 0.1366662759527415 1104 44-45 0.16565618166510632 1104 46-47 0.19726637147332582 1104 48-49 0.1517671796858906 1104 50-51 -0.13690822294152127 1104 52-53 -0.07529586042675618 1104 54-55 -0.04460838305654846 1104 56-57 0.012121598928857225 1104 58-59 0.1551709143799016 1104 60-61 0.11596405320479874 1104 62-63 -0.10561035155783571 1104 64-65 0.018663432003265257 1104 66-67 0.07438341616931865 1104 68-69 0.08368697503200195 1104 70-71 0.02321175773338524 1104 72-73 -0.07829213001623003 1104 74-75 -0.09787796454705244 1104 76-77 0.028474649722728174 1104 78-79 0.09029318280555998 1104 80-81 -0.008475244582751174 1104 82-83 0.1464683076357396 1104 84-85 0.10847964581115832 1104 86-87 0.13217858386769166 1104 88-89 0.03808146139322233 1104 90-91 -0.0961154194123317 1104 92-93 -0.044498658620032217 1104 94-95 -0.1303523465846652 1104 96-97 -0.162048879625857 1104 98-99 -0.1519232571920064 1104 100-101 0.17343001339351005 1105 1 0.014886484663904298 1105 2 0.10554523036579866 1105 3 0.13739212215509156 1105 4 0.07535143629282004 1105 5 0.04688179435486717 1105 6 -0.03659010282338926 1105 7 0.03401239142106505 1105 8 0.023039497516769814 1105 9 -0.0035900554020074082 1105 10-11 -0.012958362014195757 1105 12-13 -0.034908274216171264 1105 14-15 0.04862146479667473 1105 16-17 0.04433629476763201 1105 18-19 0.07188957296199305 1105 20-21 0.0900354643886132 1105 22-23 0.17134249758174036 1105 24-25 0.1611066447322287 1105 26-27 0.09890329570374234 1105 28-29 -0.03524829130451934 1105 30-31 0.13673136235262007 1105 32-33 0.05327483809823974 1105 34-35 0.006004059307805676 1105 36-37 -0.06460500261665203 1105 38-39 0.016809588524381525 1105 40-41 0.17734718070082778 1105 42-43 -0.003984708405951665 1105 44-45 0.18138091888849317 1105 46-47 0.15353177699447684 1105 48-49 0.1981605791746901 1105 50-51 0.12826607301762039 1105 52-53 0.1604345264369229 1105 54-55 0.16198734417516647 1105 56-57 0.23532909108087097 1105 58-59 0.18845965428231182 1105 60-61 0.10643113949393523 1105 62-63 0.19637431240247594 1105 64-65 0.16556774805507501 1105 66-67 0.2021458738539721 1105 68-69 0.1961700602823484 1105 70-71 0.32147631580850344 1105 72-73 0.27996824778067975 1105 74-75 0.2884255874364712 1105 76-77 0.3332644853819815 1105 78-79 0.3862708325551978 1105 80-81 0.3192147131816334 1105 82-83 0.29465746123761605 1105 84-85 0.431941485680305 1105 86-87 0.6019903118830747 1105 88-89 0.5671398053196306 1105 90-91 0.5203486119705119 1105 92-93 0.3123051712893421 1105 94-95 0.4025812974788856 1105 96-97 0.5761993560005969 1105 98-99 0.6895339969005683 1105 100-101 0.7707624377726177 1106 1 -0.17726951439166072 1106 2 -0.05798517302400086 1106 3 -0.059455154686574474 1106 4 -0.023695794681209748 1106 5 -0.0419186314863893 1106 6 -0.13439226435702523 1106 7 -0.1535612866473528 1106 8 -0.1038972889219778 1106 9 -0.21626780308091043 1106 10-11 -0.3155823802393911 1106 12-13 -0.39005327773241305 1106 14-15 -0.37917751900663177 1106 16-17 -0.16052713476081237 1106 18-19 -0.18810811249939974 1106 20-21 -0.15518228418733315 1106 22-23 -0.21370860844613304 1106 24-25 -0.07896241906863821 1106 26-27 -0.0870746412849499 1106 28-29 -0.14813747864335625 1106 30-31 -0.1226944585892511 1106 32-33 -0.1991852583253504 1106 34-35 -0.35757303683059405 1106 36-37 -0.13837668925096835 1106 38-39 -0.1529279673397781 1106 40-41 -0.23750212887272681 1106 42-43 -0.4973513550161073 1106 44-45 -0.3786956386013429 1106 46-47 -0.20001949532473873 1106 48-49 -0.13285081072477567 1106 50-51 -0.34174584241328887 1106 52-53 -0.2521918528715261 1106 54-55 -0.3611769770641686 1106 56-57 -0.32380726443902574 1106 58-59 -0.4515541694793299 1106 60-61 -0.4299322929964333 1106 62-63 -0.26203624273982484 1106 64-65 -0.4283639083167614 1106 66-67 -0.5542027108924188 1106 68-69 -0.32422421946455415 1106 70-71 -0.3460428584724511 1106 72-73 -0.11414668873664624 1106 74-75 -0.3560076510303958 1106 76-77 -0.44524432552063686 1106 78-79 -0.38230609009858796 1106 80-81 -0.2822969513312863 1106 82-83 -0.37980363631912795 1106 84-85 -0.4631260294530506 1106 86-87 -0.4185187427442649 1106 88-89 -0.4534343060371775 1106 90-91 -0.5769746685642616 1106 92-93 -0.4835088354350887 1106 94-95 -0.9974394148912467 1106 96-97 -0.4862567968215785 1106 98-99 -0.34369518219563844 1106 100-101 0.16004688568111192 1107 1 0.007149799516803057 1107 2 0.03002997423831033 1107 3 -0.0024678479873401216 1107 4 0.012944149246997938 1107 5 -0.0855753460668538 1107 6 -0.03424601153168538 1107 7 -0.07393064479278166 1107 8 -0.0668765295562963 1107 9 -0.07830263784476443 1107 10-11 -0.05381362384800781 1107 12-13 -0.07380657498676158 1107 14-15 -0.05139576072266294 1107 16-17 -0.05520027364846669 1107 18-19 -0.0589207841330861 1107 20-21 0.01552562216489406 1107 22-23 -0.0052948442660891715 1107 24-25 -0.07730578984335779 1107 26-27 -0.05610240470008421 1107 28-29 -0.17479127637454894 1107 30-31 -0.10339208128323207 1107 32-33 -0.07373406589701403 1107 34-35 -0.036808459560354834 1107 36-37 -0.14909676692419538 1107 38-39 -0.18310677003635334 1107 40-41 -0.05990179139146079 1107 42-43 -0.16647670238708656 1107 44-45 -0.034141996971648325 1107 46-47 -0.1266353756567682 1107 48-49 -0.15767070307876452 1107 50-51 -0.18799653387235082 1107 52-53 -0.1353832832110342 1107 54-55 -0.2012535751348139 1107 56-57 -0.22862576977126992 1107 58-59 -0.18924271005340643 1107 60-61 -0.19447796540651296 1107 62-63 -0.1653216376470752 1107 64-65 -0.16833118609371667 1107 66-67 -0.07942003832311428 1107 68-69 -0.1364573819233712 1107 70-71 -0.23559017232838642 1107 72-73 -0.19793839582391826 1107 74-75 -0.22812896536891714 1107 76-77 -0.4243425644133829 1107 78-79 -0.430324741847798 1107 80-81 -0.4309181778524547 1107 82-83 -0.17754611480387794 1107 84-85 -0.1678830626381611 1107 86-87 -0.20054663419217178 1107 88-89 -0.2301371419631728 1107 90-91 -0.18202371496629866 1107 92-93 -0.04576128488266207 1107 94-95 -0.3271662865088416 1107 96-97 -0.38888581856909354 1107 98-99 -0.12803443458271246 1107 100-101 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-0.2898070152276624 2316 86-87 -0.2679137176295079 2316 88-89 -0.30550607099113947 2316 90-91 -0.201412627807521 2316 92-93 -0.30371837742188745 2316 94-95 -0.33883510888587054 2316 96-97 -0.23516488599114282 2316 98-99 -0.2746775775327812 2316 100-101 -0.5269385861465601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 3.0 4 15.0 5 30.0 6 68.0 7 211.0 8 418.0 9 845.0 10 1516.0 11 2321.0 12 3746.0 13 5173.0 14 6714.0 15 9500.0 16 12619.0 17 17787.0 18 15712.0 19 10306.0 20 8997.0 21 9201.0 22 9563.0 23 10403.0 24 11889.0 25 14330.0 26 17447.0 27 21664.0 28 27621.0 29 34653.0 30 44391.0 31 56484.0 32 73315.0 33 98114.0 34 137780.0 35 205265.0 36 329377.0 37 493071.0 38 370035.0 39 33633.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07146488100015 18.77548836373782 15.251692217138881 22.90135453812314 2 15.88005109288639 20.11039883489119 38.774964485668924 25.2345855865535 3 19.631414962575654 23.43769174754384 29.88394274731703 27.04695054256348 4 13.505616636225811 16.485382420704557 35.99794673447218 34.01105420859745 5 14.081523200409569 37.17352298836577 33.756847844722806 14.988105966501855 6 33.372440879084145 35.71750886366078 16.270410294977854 14.639639962277215 7 29.43528035430768 32.58957370865117 19.753608136661533 18.221537800379615 8 27.609879549714094 33.72994783273048 19.02794589884086 19.63222671871456 9 26.725351860473445 16.24013656603278 19.667943988826416 37.36656758466736 10-11 25.630268953908963 26.02146378731989 26.538862825150115 21.80940443362103 12-13 26.536608030530644 23.296236482634068 27.246797997094774 22.920357489740507 14-15 23.157373252635225 24.745144385154415 25.485418233475393 26.612064128734975 16-17 22.64723226969404 27.94164905872101 26.459859852690137 22.951258818894818 18-19 22.769640320404925 26.31331399443708 28.85840824171233 22.058637443445665 20-21 23.26294930105409 25.136004966037557 29.199441320774987 22.401604412133366 22-23 23.058404894089605 24.936449464341866 29.318362457826964 22.686783183741568 24-25 23.173674768493647 25.326649218634728 29.074156045762166 22.425519967109455 26-27 22.485951055691288 25.69352971414096 29.03949080231965 22.781028427848103 28-29 22.572703687556437 26.191473298725832 28.863773433720873 22.372049579996858 30-31 22.616218410151724 25.618999868686508 28.680466521028062 23.0843152001337 32-33 22.45868996884289 25.613293700533614 28.749919421265623 23.178096909357876 34-35 23.274624264346 25.561150306199192 28.566510284234027 22.597715145220786 36-37 23.33758311547231 25.23869211760914 28.585037424345522 22.838687342573028 38-39 22.799006792488868 25.71189819623011 28.604734448304264 22.88436056297676 40-41 23.03472645011878 25.6676813618403 28.583031909178814 22.71456027886211 42-43 22.945586720403224 25.617681486246603 28.199299972921587 23.237431820428583 44-45 22.955150473325457 25.664673089090233 28.349843020687842 23.030333416896465 46-47 23.64003390275639 26.183671764017713 27.638076137950797 22.5382181952751 48-49 23.315116570569064 26.33360789790973 27.324189139180366 23.02708639234084 50-51 23.138846112523726 26.461984743759626 27.76105719299502 22.638111950721626 52-53 23.756282156883813 26.28915231171436 27.193544151177644 22.76102138022419 54-55 23.18836323699698 26.504434814788286 27.280473683582233 23.026728264632503 56-57 23.341140517375162 26.503742434552162 27.20564886771956 22.949468180353115 58-59 23.407919397390444 26.08766966300183 27.235827095942412 23.268583843665315 60-61 22.888849096921295 26.422113192231016 27.895331208442265 22.793706502405424 62-63 22.93048741181105 26.55287755613652 27.856032661247 22.66060237080543 64-65 22.712960641764855 26.744738507084957 27.48178920603087 23.060511645119316 66-67 22.88713008392126 26.56968568324798 27.290835511943556 23.252348720887202 68-69 23.161957287301984 26.1471188625864 27.66519834306247 23.025725507049145 70-71 23.171435733982737 26.360753978201963 27.22792441117836 23.239885876636944 72-73 23.138186877990968 26.38660084747462 27.276668853636338 23.198543420898073 74-75 22.867027181893064 27.062612661008252 26.973558237534174 23.096801919564516 76-77 23.224080507108834 26.32131217992336 27.2951569196242 23.1594503933436 78-79 23.153720350010147 26.62147094987406 27.187455980135848 23.037352719979946 80-81 23.41374494144612 26.726115866251238 26.857047356420633 23.00309183588201 82-83 23.3093434488133 26.666399263495265 27.034436753419893 22.989820534271537 84-85 22.937750752795587 26.877428738693744 27.084214738462624 23.10060577004805 86-87 23.17621077009395 26.99153624849288 26.659957740930416 23.172295240482757 88-89 22.92618987931096 26.93390156263057 26.52642385608041 23.613484701978056 90-91 22.864310988994458 26.605755852282996 26.93233326512226 23.597599893600282 92-93 23.08454302358896 26.319907363193586 27.288176869448954 23.307372743768504 94-95 22.76071100287696 26.1518461483365 27.534219102531964 23.55322374625458 96-97 22.771120581599398 25.907292673900844 27.634232233881267 23.687354510618487 98-99 22.456785820018908 26.46727659940216 27.010509879573302 24.065427701005625 100-101 22.232314824233516 26.208521435397238 27.522143630461766 24.03702010990748 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 143.0 2 153.5 3 240.5 4 219.0 5 225.0 6 406.5 7 938.0 8 1624.0 9 1966.0 10 1879.0 11 1718.5 12 1795.5 13 2006.0 14 2178.0 15 2344.5 16 2571.5 17 2709.5 18 2879.0 19 3134.5 20 3486.0 21 3916.5 22 4534.0 23 5417.0 24 6442.5 25 7864.5 26 9833.0 27 12329.5 28 14507.0 29 16840.5 30 19873.0 31 23878.5 32 30139.0 33 33008.0 34 32774.5 35 34955.0 36 38885.5 37 43454.0 38 48067.0 39 51221.5 40 54332.0 41 59089.0 42 61327.0 43 63355.0 44 66671.5 45 70602.0 46 74670.5 47 80691.5 48 88940.5 49 87878.0 50 80436.5 51 76870.0 52 77311.5 53 80021.5 54 82106.0 55 77207.0 56 69590.0 57 64679.5 58 60900.0 59 54628.5 60 44298.5 61 36488.0 62 30762.0 63 22560.5 64 14968.5 65 10865.5 66 8084.5 67 6353.5 68 4966.0 69 3737.5 70 2774.5 71 1833.5 72 1281.5 73 1021.0 74 747.0 75 547.0 76 366.5 77 211.5 78 133.5 79 95.5 80 61.0 81 41.0 82 27.5 83 16.5 84 8.0 85 4.5 86 4.0 87 3.0 88 1.5 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05314615191777388 2 0.0 3 0.0 4 0.0 5 0.015184614833649678 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031992741945110962 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012176342083587007 24-25 2.8650216667263544E-4 26-27 0.0013608852916950184 28-29 0.0035335267222958372 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014611610500304408 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.4325108333631772E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.297532500089532E-4 84-85 0.0014802611944752832 86-87 0.0 88-89 0.0 90-91 0.011197459680788835 92-93 0.0011937590278026477 94-95 0.0 96-97 0.0 98-99 2.3875180556052957E-4 100-101 0.013465601833613866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2094225.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.373319295426384 #Duplication Level Percentage of deduplicated Percentage of total 1 67.69090525042755 29.35979246823248 2 15.440265400523748 13.393911224460822 3 6.427710322033953 8.363733964082597 4 3.212096610174776 5.572771675234691 5 1.9133188179561247 4.149349400257939 6 1.17382601830664 3.054763841357575 7 0.81306629263682 2.4685768743219763 8 0.5744207505260225 1.993162769798691 9 0.4167443318051105 1.6268026471147958 >10 2.1381108841507106 16.49497455918715 >50 0.1306802547350785 3.885007947348492 >100 0.0602644192558581 4.691539187765215 >500 0.004846006263839436 1.4460413838269044 >1k 0.0035243681918832215 2.8071001895484224 >5k 2.2027301199270134E-4 0.6924718674624157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8120 0.3877329322303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6376 0.3044563024507873 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4414 0.21077009394883547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4398 0.21000608817104177 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2895 0.1382372954195466 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2510 0.11985340639138584 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2458 0.11737038761355634 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2447 0.11684513364132318 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2437 0.1163676300302021 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2402 0.1146963673912784 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2210 0.10552829805775407 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2098 0.1001802576131982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4325108333631772E-4 2 0.0 0.0 0.0 0.0 1.4325108333631772E-4 3 0.0 0.0 0.0 0.0 1.4325108333631772E-4 4 0.0 0.0 0.0 0.0 1.4325108333631772E-4 5 0.0 0.0 0.0 4.775036111210591E-5 1.4325108333631772E-4 6 0.0 0.0 0.0 4.775036111210591E-5 1.4325108333631772E-4 7 0.0 0.0 0.0 4.775036111210591E-5 1.4325108333631772E-4 8 0.0 0.0 0.0 4.775036111210591E-5 1.4325108333631772E-4 9 0.0 0.0 0.0 4.775036111210591E-5 1.4325108333631772E-4 10-11 0.0 0.0 0.0 4.775036111210591E-5 1.4325108333631772E-4 12-13 0.0 0.0 0.0 4.775036111210591E-5 1.9100144444842364E-4 14-15 2.3875180556052956E-5 0.0 0.0 4.775036111210591E-5 1.9100144444842364E-4 16-17 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 2.1487662500447658E-4 18-19 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 2.3875180556052954E-4 20-21 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 3.3425252778474136E-4 22-23 4.775036111210591E-5 0.0 0.0 4.775036111210591E-5 3.3425252778474136E-4 24-25 9.550072222421182E-5 0.0 0.0 1.1937590278026477E-4 3.3425252778474136E-4 26-27 9.550072222421182E-5 0.0 0.0 2.1487662500447658E-4 3.3425252778474136E-4 28-29 9.550072222421182E-5 0.0 0.0 6.207546944573769E-4 3.3425252778474136E-4 30-31 9.550072222421182E-5 0.0 0.0 0.0015280115555873892 3.3425252778474136E-4 32-33 9.550072222421182E-5 0.0 0.0 0.002602394680609772 3.820028888968473E-4 34-35 1.4325108333631772E-4 0.0 0.0 0.0033664004584034665 4.297532500089532E-4 36-37 1.9100144444842364E-4 0.0 0.0 0.004106531055641108 4.297532500089532E-4 38-39 2.3875180556052954E-4 0.0 0.0 0.005777793694564815 4.297532500089532E-4 40-41 2.3875180556052954E-4 0.0 0.0 0.008642815361291169 4.297532500089532E-4 42-43 2.626269861165825E-4 0.0 0.0 0.012319593166923326 4.297532500089532E-4 44-45 3.3425252778474136E-4 0.0 0.0 0.019888025403192112 4.297532500089532E-4 46-47 3.3425252778474136E-4 0.0 0.0 0.037245281667442606 4.297532500089532E-4 48-49 3.3425252778474136E-4 0.0 0.0 0.051642015542742545 4.297532500089532E-4 50-51 3.3425252778474136E-4 0.0 0.0 0.07067053444591676 4.775036111210591E-4 52-53 3.3425252778474136E-4 0.0 0.0 0.09509484415475891 5.730043333452709E-4 54-55 3.3425252778474136E-4 0.0 0.0 0.12658620730819276 5.730043333452709E-4 56-57 3.3425252778474136E-4 0.0 0.0 0.16017858635055926 5.730043333452709E-4 58-59 3.3425252778474136E-4 0.0 0.0 0.20470579808759803 5.730043333452709E-4 60-61 3.3425252778474136E-4 0.0 0.0 0.26475187718607124 5.730043333452709E-4 62-63 3.3425252778474136E-4 0.0 0.0 0.3403168236459788 7.162554166815887E-4 64-65 3.820028888968473E-4 0.0 0.0 0.4026787952583891 7.640057777936946E-4 66-67 4.297532500089532E-4 0.0 0.0 0.5086845969272642 7.640057777936946E-4 68-69 4.297532500089532E-4 0.0 0.0 0.6396877126383269 7.640057777936946E-4 70-71 4.5362843056500615E-4 0.0 0.0 0.7352123100430947 8.356313194618534E-4 72-73 4.775036111210591E-4 0.0 0.0 0.9018371951437882 9.311320416860653E-4 74-75 4.775036111210591E-4 0.0 0.0 1.0461387864245721 9.550072222421182E-4 76-77 5.25253972233165E-4 0.0 0.0 1.1794577946495721 9.550072222421182E-4 78-79 5.25253972233165E-4 0.0 0.0 1.3511919683892608 9.550072222421182E-4 80-81 5.25253972233165E-4 0.0 0.0 1.5277728037818286 9.550072222421182E-4 82-83 5.730043333452709E-4 0.0 0.0 1.7468514605641705 9.550072222421182E-4 84-85 5.968795139013239E-4 0.0 0.0 2.0544592868483567 9.550072222421182E-4 86-87 6.207546944573769E-4 0.0 0.0 2.4392794470508186 9.550072222421182E-4 88-89 6.207546944573769E-4 0.0 0.0 2.89075911136578 9.550072222421182E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12410 0.0 27.492138 1 TAAGGTG 1205 0.0 25.624634 5 AGGTAAG 1065 0.0 24.978699 2 GTAAGGT 1165 0.0 24.873407 4 GTACATG 7530 0.0 24.169052 1 GGTATCA 8980 0.0 24.129278 1 TACATGG 7635 0.0 23.394306 2 TCAACGC 14985 0.0 22.666302 4 ATCAACG 15000 0.0 22.611965 3 CAACGCA 15100 0.0 22.525137 5 TATCAAC 15240 0.0 22.255873 2 GAGTACT 8290 0.0 22.231415 12-13 GGTAAGG 1335 0.0 22.061846 3 AGAGTAC 12060 0.0 22.036503 10-11 AACGCAG 15535 0.0 21.922365 6 ACATGGG 7670 0.0 21.677244 3 AGTACTT 8600 0.0 21.347202 12-13 GTACTTT 8480 0.0 21.144657 14-15 ACCGAGT 620 0.0 20.685308 8 CCCTATA 400 0.0 20.189257 2 >>END_MODULE