FastQCFastQC Report
Thu 26 May 2016
SRR937220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277799
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69940.30705079772183586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.2237247448084752No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42880.18825190457981586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35400.1554131861503144No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33050.14509620910361276No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25230.1107648216545885No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25210.11067701759461657No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24560.10782338564552887No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24530.10769167955557096No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24420.10720875722572536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG12950.027.8819525
GTATCAA127050.027.5239751
GGTATCA91600.026.9203031
AGGTAAG12300.026.6498432
GTAAGGT13650.025.059894
TCAACGC150050.023.1135254
ATCAACG150400.023.08983
TATCAAC151100.022.9513932
CAACGCA150950.022.9442445
GAGTACT74050.022.8055912-13
GTACATG88500.022.6556851
GTACTTT75050.022.53262114-15
AACGCAG154550.022.5317636
GGTAAGG15600.021.621433
TACATGG89700.021.608192
AGAGTAC120650.020.92725610-11
AGTACTT77750.020.80383912-13
ACGCAGA170150.020.326367
CGCAGAG171200.020.0620678
AAGGTAA16000.019.8958661
Produced by FastQC (version 0.11.5)