##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937220_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2277799 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782179200184036 33.0 31.0 34.0 30.0 34.0 2 31.881362227308028 33.0 31.0 34.0 30.0 34.0 3 31.93164497833215 33.0 31.0 34.0 30.0 34.0 4 35.54589452361688 37.0 35.0 37.0 33.0 37.0 5 35.55627559762736 37.0 35.0 37.0 33.0 37.0 6 35.5824135492201 37.0 35.0 37.0 33.0 37.0 7 35.47063810283524 37.0 35.0 37.0 33.0 37.0 8 35.46652360458495 37.0 35.0 37.0 33.0 37.0 9 37.09621568891724 39.0 37.0 39.0 33.0 39.0 10-11 36.98478860514031 39.0 37.0 39.0 33.0 39.0 12-13 36.842980438572496 39.0 37.0 39.0 33.0 39.0 14-15 38.03329178737896 40.0 38.0 41.0 33.0 41.0 16-17 37.968667560219316 40.0 38.0 41.0 33.0 41.0 18-19 37.91333212456411 40.0 38.0 41.0 33.0 41.0 20-21 37.77374496169328 40.0 38.0 41.0 32.5 41.0 22-23 37.626235897021644 40.0 37.0 41.0 32.0 41.0 24-25 37.555605433139625 40.0 37.0 41.0 32.0 41.0 26-27 37.57470610883577 40.0 37.0 41.0 32.0 41.0 28-29 37.49390727627855 40.0 37.0 41.0 32.0 41.0 30-31 37.27520536272077 39.5 37.0 41.0 31.5 41.0 32-33 37.04060103635132 39.0 36.0 41.0 31.0 41.0 34-35 37.090854373015354 39.5 36.0 41.0 31.0 41.0 36-37 36.964603988323816 39.5 36.0 41.0 30.5 41.0 38-39 36.844997078319906 39.0 36.0 41.0 30.5 41.0 40-41 36.62913167491952 39.0 35.5 41.0 30.0 41.0 42-43 36.35230610778211 39.0 35.0 41.0 30.0 41.0 44-45 36.13456520966073 39.0 35.0 41.0 29.5 41.0 46-47 35.82765182529275 39.0 35.0 40.5 28.5 41.0 48-49 35.49980441645641 39.0 35.0 40.0 27.0 41.0 50-51 34.49583194127313 38.0 34.0 39.5 25.0 40.5 52-53 34.5975880663746 38.0 34.0 39.5 26.0 40.5 54-55 34.95322699676311 38.0 34.0 40.0 26.0 41.0 56-57 34.69248208467911 38.0 34.0 40.0 25.5 41.0 58-59 34.383565011662576 38.0 34.0 40.0 25.0 41.0 60-61 34.04620864264143 37.0 33.5 40.0 24.0 41.0 62-63 33.70155290260466 37.0 33.0 40.0 23.0 41.0 64-65 33.24751569387817 36.0 33.0 39.0 22.0 41.0 66-67 32.864009291425624 36.0 32.0 39.0 21.5 40.5 68-69 32.36531691338876 35.0 32.0 38.5 20.0 40.0 70-71 31.871677000472825 35.0 31.0 37.5 18.0 40.0 72-73 31.408273074138677 35.0 31.0 37.0 17.0 39.0 74-75 30.927219434199415 35.0 30.5 36.5 12.0 39.0 76-77 30.456486722489558 34.0 30.0 36.0 7.5 38.5 78-79 29.966887771923687 34.0 29.5 35.5 7.0 37.5 80-81 29.507703050181338 34.0 29.0 35.0 2.0 37.0 82-83 29.0452070178273 34.0 29.0 35.0 2.0 36.5 84-85 28.53965692319647 34.0 28.5 35.0 2.0 36.0 86-87 28.11066955424952 33.5 27.0 35.0 2.0 35.5 88-89 27.633673778941862 33.0 26.5 35.0 2.0 35.0 90-91 27.126795208883664 33.0 25.5 35.0 2.0 35.0 92-93 26.624641155782403 33.0 25.0 35.0 2.0 35.0 94-95 26.063075802562032 32.0 24.5 34.5 2.0 35.0 96-97 25.30801159364808 32.0 21.0 34.0 2.0 35.0 98-99 24.345374855287933 31.5 10.0 34.0 2.0 35.0 100-101 21.761894706249322 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11169996566786722 1101 2 -0.08528217302168528 1101 3 -0.06950264629722369 1101 4 -0.04278059140267487 1101 5 -0.08897058811906078 1101 6 -0.1573066790237263 1101 7 -0.031422594792466896 1101 8 -0.1829486310986539 1101 9 -0.2044833530893797 1101 10-11 -0.16827213052506096 1101 12-13 -0.14563090848985638 1101 14-15 -0.21651399524513693 1101 16-17 -0.19152020196602848 1101 18-19 -0.06288766054316852 1101 20-21 -0.05399136435077878 1101 22-23 -0.2220100727024601 1101 24-25 -0.18148203329899104 1101 26-27 -0.1870616478953835 1101 28-29 -0.22253655348384171 1101 30-31 -0.11577972141785153 1101 32-33 -0.298597532501951 1101 34-35 -0.24885625769456965 1101 36-37 -0.14557265946285725 1101 38-39 -0.09050912464613958 1101 40-41 -0.31380040699704637 1101 42-43 -0.1876847051236794 1101 44-45 -0.15910795436683145 1101 46-47 -0.1144744181727404 1101 48-49 -0.14167984461484906 1101 50-51 -0.20616207831363198 1101 52-53 -0.13398419866538092 1101 54-55 -0.2710003343034586 1101 56-57 -0.32792651023503794 1101 58-59 -0.17173165847843563 1101 60-61 -0.16366262307864332 1101 62-63 -0.21428841255188047 1101 64-65 -0.360250108231142 1101 66-67 -0.2712677040225131 1101 68-69 -0.2757821274274903 1101 70-71 -0.3560897706676336 1101 72-73 -0.37490939617122976 1101 74-75 -0.4571937206304959 1101 76-77 -0.40195308228927473 1101 78-79 -0.4288831664944581 1101 80-81 -0.48380950422478364 1101 82-83 -0.22559955227452733 1101 84-85 -0.2675983270726121 1101 86-87 -0.189826271383307 1101 88-89 -0.36304570045293616 1101 90-91 -0.34239189929023084 1101 92-93 -0.1144741189364531 1101 94-95 -0.1612376630140666 1101 96-97 -0.18945552518244213 1101 98-99 0.05745049450183615 1101 100-101 0.26604490679102 1102 1 -0.11127542634639553 1102 2 -0.06592353572175469 1102 3 -0.017795565384535195 1102 4 -0.12357054286525937 1102 5 -0.06261120253892472 1102 6 -0.21405916666999047 1102 7 -0.10917688258032143 1102 8 -0.09016381324143907 1102 9 -0.1619292154319112 1102 10-11 -0.15088862387769808 1102 12-13 -0.09620542996406556 1102 14-15 -0.10746082762444331 1102 16-17 -0.05647541484640328 1102 18-19 -0.12732425061487618 1102 20-21 -0.17694886179820912 1102 22-23 -0.1941945202143387 1102 24-25 -0.08975708141542071 1102 26-27 -0.22165493211022635 1102 28-29 -0.22286096141158396 1102 30-31 -0.26202180725780977 1102 32-33 -0.2128685314856611 1102 34-35 -0.04018510134669384 1102 36-37 -0.09120805726687564 1102 38-39 0.0536363535950386 1102 40-41 -0.04173859264879809 1102 42-43 -0.039836811158899366 1102 44-45 0.0942817638362925 1102 46-47 0.08391707586176977 1102 48-49 -0.148487519408242 1102 50-51 -0.018091307134397994 1102 52-53 -0.26622753025549883 1102 54-55 -0.08738952361124319 1102 56-57 -0.1307276989451438 1102 58-59 -0.22283372638812438 1102 60-61 -0.043773029984158995 1102 62-63 -0.10118151698974032 1102 64-65 -0.12641313844577695 1102 66-67 -0.28932366968938084 1102 68-69 -0.2700968729351416 1102 70-71 -0.35117547602548527 1102 72-73 -0.22065279654145797 1102 74-75 -0.34093198205896513 1102 76-77 -0.3161323991604412 1102 78-79 -0.3993261726834838 1102 80-81 -0.2068657100291169 1102 82-83 -0.37601565713810814 1102 84-85 -0.37881999529868793 1102 86-87 -0.38460132483612597 1102 88-89 -0.4327685017720917 1102 90-91 -0.44001215183183007 1102 92-93 -0.22515229085590605 1102 94-95 -0.19636651306985797 1102 96-97 0.05697724759296463 1102 98-99 0.040076993582200515 1102 100-101 0.3629720218091599 1103 1 -0.01804723915142148 1103 2 -0.09491637131538866 1103 3 -0.11783590423084433 1103 4 -0.045762021710089584 1103 5 -0.015607924297476927 1103 6 -0.06539631420221781 1103 7 0.0055331606561068725 1103 8 -0.04417184692153597 1103 9 -0.09021697344428503 1103 10-11 -0.05001310046696261 1103 12-13 -0.01852474318101116 1103 14-15 -0.23226978625724115 1103 16-17 -0.0895646318750849 1103 18-19 -0.11105519619152204 1103 20-21 -0.05767087033186158 1103 22-23 -0.08340757181965586 1103 24-25 -0.20165192770169682 1103 26-27 -0.26682307692100693 1103 28-29 -0.3375340747182918 1103 30-31 -0.29010275766374605 1103 32-33 -0.2888902121951702 1103 34-35 -0.04286110086945172 1103 36-37 -0.05826371919324913 1103 38-39 -0.18132038157191488 1103 40-41 0.06855122207658582 1103 42-43 0.004726250957240552 1103 44-45 -0.16172503127076965 1103 46-47 0.07435222406441966 1103 48-49 0.12193899287237997 1103 50-51 0.06221424542257381 1103 52-53 0.22890055978535173 1103 54-55 0.2579734975979022 1103 56-57 0.23736995515209713 1103 58-59 0.10740506821896645 1103 60-61 0.11508532385001757 1103 62-63 0.22381038683335674 1103 64-65 0.08076092651680966 1103 66-67 0.20340113415587524 1103 68-69 0.19100170725110388 1103 70-71 0.20347513843173104 1103 72-73 0.08664114865452532 1103 74-75 0.10094314932636195 1103 76-77 0.1788706972721279 1103 78-79 0.09998636247096826 1103 80-81 0.01830459376217064 1103 82-83 0.11047935163984945 1103 84-85 -0.006336201856310453 1103 86-87 0.08336338151411837 1103 88-89 0.16635995188652686 1103 90-91 0.3180911269777731 1103 92-93 0.12892903453086646 1103 94-95 -0.22148454680677077 1103 96-97 -0.1469952765393252 1103 98-99 0.07365766275252739 1103 100-101 0.3631907606236524 1104 1 0.02306553113355747 1104 2 -0.03872360654839824 1104 3 -0.001544306021443731 1104 4 -0.007429657075689988 1104 5 -0.004270224082439711 1104 6 0.05400748810997413 1104 7 0.03037211582114452 1104 8 -0.02523203941040464 1104 9 0.10393922222984742 1104 10-11 0.15659362781723019 1104 12-13 0.018451906053435607 1104 14-15 0.1297502746367627 1104 16-17 0.0910439714789888 1104 18-19 0.16788996912909937 1104 20-21 0.279840562821704 1104 22-23 0.14065477883017508 1104 24-25 0.019166643597053223 1104 26-27 0.04341493207906666 1104 28-29 0.04754573343355872 1104 30-31 -0.027347477164937573 1104 32-33 -0.025131118792764084 1104 34-35 0.01882488965189566 1104 36-37 -0.0199585685698338 1104 38-39 0.08426000747240181 1104 40-41 0.12678046113687458 1104 42-43 0.016236388298921156 1104 44-45 0.029461813427289485 1104 46-47 -0.06014473025461342 1104 48-49 -0.11510321515968513 1104 50-51 -0.1569092117146198 1104 52-53 -0.13082633249554476 1104 54-55 -0.12881809636186148 1104 56-57 -0.19507775962284057 1104 58-59 -0.08747340201691856 1104 60-61 -0.1260935772988887 1104 62-63 -0.011521342970901571 1104 64-65 -0.06641424279846575 1104 66-67 -0.1740621015949344 1104 68-69 -0.231222038665166 1104 70-71 -0.10477796396552108 1104 72-73 -0.08018237314250598 1104 74-75 -0.1390671838254356 1104 76-77 -0.1312033738720011 1104 78-79 -0.10032592034814769 1104 80-81 -0.009508860975618205 1104 82-83 0.18221581148439014 1104 84-85 0.2001751548038193 1104 86-87 0.2841244094812936 1104 88-89 0.04022930371253253 1104 90-91 0.21654788824568172 1104 92-93 0.2740251514756018 1104 94-95 0.4025987578142818 1104 96-97 0.6172541594033056 1104 98-99 0.7091757554115752 1104 100-101 0.9259613071202182 1105 1 0.048504877095879806 1105 2 0.02873087724251633 1105 3 -0.16327384044027582 1105 4 -0.06912495039149746 1105 5 0.017686801702737398 1105 6 -0.042902496921769284 1105 7 0.02368515450707065 1105 8 -0.03354300732485882 1105 9 -0.02466237112281533 1105 10-11 -0.07100934822377525 1105 12-13 -0.015325556126448703 1105 14-15 -0.031018389210174746 1105 16-17 -0.09311144725708687 1105 18-19 0.03547416147930704 1105 20-21 -0.018174594573238778 1105 22-23 0.0547151540474502 1105 24-25 0.09505992875369174 1105 26-27 0.10359929596090467 1105 28-29 -0.08925391372002878 1105 30-31 -0.18365974298025378 1105 32-33 -0.017708381395934225 1105 34-35 -0.014653533561613585 1105 36-37 0.04013676844414249 1105 38-39 0.1067822560367162 1105 40-41 0.031245256391386533 1105 42-43 0.12419059653047526 1105 44-45 0.1624529531342489 1105 46-47 0.09266687193551348 1105 48-49 0.03187574123671055 1105 50-51 0.053810373217984875 1105 52-53 0.07798912100340516 1105 54-55 0.19426798903219122 1105 56-57 0.03150515765391759 1105 58-59 0.09713473301695075 1105 60-61 0.35413332707712897 1105 62-63 0.2581909339320063 1105 64-65 0.3056875739366305 1105 66-67 0.25772619707879585 1105 68-69 0.18857950968490655 1105 70-71 0.17745033288201384 1105 72-73 0.35841077407140887 1105 74-75 0.3963729284853805 1105 76-77 0.380702665280257 1105 78-79 0.3930986465018975 1105 80-81 0.4175129946312772 1105 82-83 0.7136520790085008 1105 84-85 0.7041807116456802 1105 86-87 0.6956966794055397 1105 88-89 0.6991473174676734 1105 90-91 0.7933664984482256 1105 92-93 0.9554121770325068 1105 94-95 1.033923334121397 1105 96-97 1.109068171514874 1105 98-99 1.0967141135995995 1105 100-101 1.2896401386181218 1106 1 -0.2393790933937261 1106 2 -0.05643092691576612 1106 3 -0.06963115097012107 1106 4 0.11975834940729158 1106 5 0.0060410941239297244 1106 6 -0.1704569905502069 1106 7 -0.4281827194030612 1106 8 -0.27206078063136374 1106 9 -0.45218584530121575 1106 10-11 -0.6972090464129153 1106 12-13 -0.21915310475153404 1106 14-15 -0.2675245092638292 1106 16-17 -0.16353733592241326 1106 18-19 -0.54618789418803 1106 20-21 -0.32087999363427144 1106 22-23 -0.3665369418613338 1106 24-25 -0.2761997124267239 1106 26-27 -0.4366819749981872 1106 28-29 -0.25879239460533654 1106 30-31 -0.3589125095798451 1106 32-33 -0.16566528951129555 1106 34-35 0.004564692461194397 1106 36-37 -0.27531175603606783 1106 38-39 -0.17276963866482475 1106 40-41 -0.2783778992088841 1106 42-43 -0.199207290793467 1106 44-45 -0.1419681568590434 1106 46-47 -0.24158111599205512 1106 48-49 0.20053394043188888 1106 50-51 -0.04963949264384837 1106 52-53 0.16307966426296616 1106 54-55 -0.035057966702218835 1106 56-57 -0.16330034201073573 1106 58-59 -0.12622542794884595 1106 60-61 -0.21357500625621384 1106 62-63 -0.20962135647710767 1106 64-65 -0.03795260044297777 1106 66-67 -0.05717697193528437 1106 68-69 -0.16031678474836042 1106 70-71 0.045493173257600716 1106 72-73 -0.19960565785347484 1106 74-75 0.14311001768725973 1106 76-77 0.40198578063907675 1106 78-79 0.4681573319479071 1106 80-81 0.007117287052473387 1106 82-83 0.1416006034887154 1106 84-85 0.08129380688377452 1106 86-87 0.2218209248783758 1106 88-89 0.5489188634099911 1106 90-91 0.6677658109901436 1106 92-93 0.8723638872940747 1106 94-95 0.7768211376089873 1106 96-97 0.8351161259078985 1106 98-99 0.9666210552495009 1106 100-101 1.4051414799997453 1107 1 0.05734654033272335 1107 2 -0.016921810743880883 1107 3 -0.011541649817889521 1107 4 0.08282260316898515 1107 5 -0.05266552780910416 1107 6 -0.006696484924617607 1107 7 0.15788817662513566 1107 8 0.07686290108207317 1107 9 0.19458282760104595 1107 10-11 0.14007186045979836 1107 12-13 0.14387192235975732 1107 14-15 0.02141094891700135 1107 16-17 -0.036611078636255456 1107 18-19 -0.02270688903688267 1107 20-21 0.14688620536260544 1107 22-23 0.1597333068847675 1107 24-25 0.1947436758069827 1107 26-27 0.03519726114116395 1107 28-29 0.056696371153101666 1107 30-31 0.07995161201429823 1107 32-33 0.1492460904534596 1107 34-35 0.1333679830942387 1107 36-37 0.01239479811873423 1107 38-39 0.06981821886533623 1107 40-41 0.05269833435490057 1107 42-43 0.0305919162012529 1107 44-45 0.022181311081851618 1107 46-47 0.09277177136053183 1107 48-49 -0.13113518319561024 1107 50-51 -0.11111688860426483 1107 52-53 -0.15389064887245496 1107 54-55 -0.12963909724309275 1107 56-57 -0.17028353300978694 1107 58-59 -0.1483604421822733 1107 60-61 -0.034886599722391054 1107 62-63 0.046725312222896775 1107 64-65 0.10937320950686313 1107 66-67 0.16666834120012908 1107 68-69 0.1339584118085071 1107 70-71 0.28510946375509505 1107 72-73 0.1655383708844944 1107 74-75 0.25901146814137377 1107 76-77 0.11000470770970239 1107 78-79 0.2439689762477606 1107 80-81 0.39043028691691717 1107 82-83 0.5208979126929982 1107 84-85 0.5445862191109683 1107 86-87 0.6915398146709748 1107 88-89 0.7298998685611373 1107 90-91 0.6819798376947688 1107 92-93 0.7578966241789864 1107 94-95 0.9772566974571646 1107 96-97 1.0201218870574174 1107 98-99 1.218641693720425 1107 100-101 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0.24605573707800232 2310 9 0.3569046876348949 2310 10-11 0.3158582326337651 2310 12-13 0.3212848710842806 2310 14-15 0.24339881910426442 2310 16-17 0.39371357437219245 2310 18-19 0.26668925741740424 2310 20-21 0.43774173758055923 2310 22-23 0.37664184827438163 2310 24-25 0.4568661184239957 2310 26-27 0.4826959213937343 2310 28-29 0.6000749092675477 2310 30-31 0.6134006731411077 2310 32-33 0.499351675069974 2310 34-35 0.540468159856097 2310 36-37 0.712737317118517 2310 38-39 0.6518639677568885 2310 40-41 0.6025197754021718 2310 42-43 0.7915486674984962 2310 44-45 0.7412652509529352 2310 46-47 0.7448228065501539 2310 48-49 0.6929700129244409 2310 50-51 0.808356617915571 2310 52-53 0.8153736066661352 2310 54-55 0.6807066807655104 2310 56-57 0.8791210416370561 2310 58-59 0.9409145919121329 2310 60-61 0.7488205167494186 2310 62-63 0.9361919089978912 2310 64-65 0.9672749732081272 2310 66-67 0.8480992197231032 2310 68-69 0.8872454876779869 2310 70-71 0.8579201428041081 2310 72-73 0.92518000914421 2310 74-75 0.9528137581705423 2310 76-77 0.8461675499207679 2310 78-79 0.9009982786542992 2310 80-81 0.9459123880124203 2310 82-83 1.04417963361119 2310 84-85 1.0539040766619934 2310 86-87 1.244366853378871 2310 88-89 1.2097130954523614 2310 90-91 1.2582124760017308 2310 92-93 1.4853976509484959 2310 94-95 1.4806677529681416 2310 96-97 1.4109147025381539 2310 98-99 1.7157211049019345 2310 100-101 1.7184193681162618 2311 1 0.43096369620438324 2311 2 0.518134675921111 2311 3 0.500046745010966 2311 4 0.39688600898175963 2311 5 0.350789912090832 2311 6 0.28980010164837466 2311 7 0.38695912456856973 2311 8 0.3549841366263209 2311 9 0.28726450930871295 2311 10-11 0.4738991096154521 2311 12-13 0.4462473089600465 2311 14-15 0.6734285049205653 2311 16-17 0.689098597883735 2311 18-19 0.6297119285070067 2311 20-21 0.7264929378078122 2311 22-23 0.6782724553017871 2311 24-25 0.6282934908829745 2311 26-27 0.6048591006519288 2311 28-29 0.7965016361275232 2311 30-31 0.6239465329733491 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1.4180838212069489 2311 100-101 1.737854245957191 2312 1 0.4701576335542548 2312 2 0.44117149549995105 2312 3 0.4738682350535477 2312 4 0.3951937144441331 2312 5 0.48817968532732436 2312 6 0.508551437670377 2312 7 0.48999432904525264 2312 8 0.5487184111525139 2312 9 0.6438149361232064 2312 10-11 0.6058493929708746 2312 12-13 0.7302271977713488 2312 14-15 0.8291071328437809 2312 16-17 0.8104624408134669 2312 18-19 0.8343904994266182 2312 20-21 0.8848196603063414 2312 22-23 0.9035667364150655 2312 24-25 0.9455508178255698 2312 26-27 1.0360791044838393 2312 28-29 0.8458178699626444 2312 30-31 0.9314043859325452 2312 32-33 1.0889359049517537 2312 34-35 0.4078574196324354 2312 36-37 0.7151389584091206 2312 38-39 1.0149328339853199 2312 40-41 1.0515217714765441 2312 42-43 1.134807277190454 2312 44-45 1.2290832776129363 2312 46-47 1.1278537467005165 2312 48-49 0.9823854976991342 2312 50-51 1.065591306641707 2312 52-53 0.973454747987013 2312 54-55 0.9629159113955765 2312 56-57 0.9681118035574698 2312 58-59 1.0418187783471993 2312 60-61 1.0726865755700956 2312 62-63 1.0559648513817805 2312 64-65 1.182957759012261 2312 66-67 1.4244535538517127 2312 68-69 1.2233630265934607 2312 70-71 1.2382193677206494 2312 72-73 1.1931767712601662 2312 74-75 1.2186034313956746 2312 76-77 1.340100175593303 2312 78-79 1.524115693189259 2312 80-81 1.3982537014306828 2312 82-83 1.4057630839531008 2312 84-85 1.5744132252807397 2312 86-87 1.5774279242085854 2312 88-89 1.4704533856247721 2312 90-91 1.595891090070296 2312 92-93 1.6886230969390859 2312 94-95 1.8487382787119095 2312 96-97 1.8706179120208688 2312 98-99 1.9406875321416628 2312 100-101 1.9476370147173157 2313 1 0.4781795760070473 2313 2 0.5428567809903626 2313 3 0.5082615922515679 2313 4 0.49944050935260975 2313 5 0.42490330647298435 2313 6 0.4912805920426351 2313 7 0.4100184653770711 2313 8 0.5075177110523938 2313 9 0.5224923274032705 2313 10-11 0.7282378093628878 2313 12-13 0.7365242989850813 2313 14-15 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2315 3 0.3279333102982598 2315 4 0.22026855348892838 2315 5 0.33068367696351686 2315 6 0.29698568006642745 2315 7 0.3542936905157461 2315 8 0.2856328774770418 2315 9 0.3525019975778676 2315 10-11 0.39475853094060653 2315 12-13 0.44592767756139295 2315 14-15 0.3473513685066294 2315 16-17 0.2956979065616778 2315 18-19 0.41292596909921997 2315 20-21 0.5017634669757811 2315 22-23 0.466891437554942 2315 24-25 0.4328878177466393 2315 26-27 0.5107046005997944 2315 28-29 0.5329780958489039 2315 30-31 0.7238915555946122 2315 32-33 0.6325081250158036 2315 34-35 0.5826630850440324 2315 36-37 0.6958285226560577 2315 38-39 0.6744342509555352 2315 40-41 0.6497572500121009 2315 42-43 0.8108330317871832 2315 44-45 0.762786753169479 2315 46-47 0.8272133211376413 2315 48-49 0.9248677949414272 2315 50-51 0.7876122628970705 2315 52-53 0.7808347663037765 2315 54-55 0.9003765296769828 2315 56-57 0.910736304906095 2315 58-59 0.9865922152374651 2315 60-61 1.0818489294470979 2315 62-63 1.1242675781289861 2315 64-65 1.0295199692300514 2315 66-67 1.2309554342529907 2315 68-69 1.2104656653284422 2315 70-71 1.1445302316293358 2315 72-73 1.1784100322935522 2315 74-75 1.3103702766295449 2315 76-77 1.3978368065333022 2315 78-79 1.1431436167844247 2315 80-81 1.2136566770020032 2315 82-83 1.2490728988584756 2315 84-85 1.3228113897233627 2315 86-87 1.33930858123124 2315 88-89 1.4555261434187692 2315 90-91 1.5221808592675181 2315 92-93 1.435908982751613 2315 94-95 1.7389015907579868 2315 96-97 1.993013317042415 2315 98-99 1.7680323455720846 2315 100-101 1.6125174949218426 2316 1 -0.1620676220107704 2316 2 -0.05826063706069462 2316 3 -0.027355604421362756 2316 4 -0.024659484048861202 2316 5 -0.06121787173212567 2316 6 -0.05343199669120935 2316 7 0.1004920533800373 2316 8 -0.11135579414155927 2316 9 -0.1358292989980967 2316 10-11 -0.6134977346958905 2316 12-13 -0.08753449138872327 2316 14-15 -0.021155527441180766 2316 16-17 -0.15501240347338552 2316 18-19 -0.08560188952087344 2316 20-21 -0.003670769856327638 2316 22-23 0.028279902894247755 2316 24-25 0.039677375512361834 2316 26-27 0.1097984659254223 2316 28-29 -0.03101190455375047 2316 30-31 0.15754889425213037 2316 32-33 0.2355454081059989 2316 34-35 -0.0280604303587495 2316 36-37 -0.14979990391128695 2316 38-39 -0.05741115794755558 2316 40-41 0.12484873429639975 2316 42-43 0.06111234811589128 2316 44-45 0.0056379288692767204 2316 46-47 -0.041878647311136774 2316 48-49 0.019096486489367237 2316 50-51 0.0030671519152321025 2316 52-53 -0.07596970935531289 2316 54-55 0.09189487043853006 2316 56-57 0.13592712052918188 2316 58-59 0.21799712179323905 2316 60-61 0.11220259752663964 2316 62-63 0.14019333892942143 2316 64-65 0.24267071078276103 2316 66-67 0.17017702585395256 2316 68-69 0.36829212583872106 2316 70-71 0.19359821501638308 2316 72-73 0.03801470569356624 2316 74-75 0.2496231594214784 2316 76-77 0.15764271382502315 2316 78-79 0.49621861910094367 2316 80-81 0.43160758427674395 2316 82-83 0.47573318762037786 2316 84-85 0.44048979681253897 2316 86-87 0.36322936260866356 2316 88-89 0.5008294811943195 2316 90-91 0.602845183380218 2316 92-93 0.7655658032852344 2316 94-95 0.9839205910602296 2316 96-97 0.8438639765588434 2316 98-99 0.8240501267307074 2316 100-101 0.7144113154210174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8166.0 3 1954.0 4 1409.0 5 1780.0 6 1925.0 7 2379.0 8 2964.0 9 3539.0 10 4804.0 11 5944.0 12 7951.0 13 9835.0 14 11061.0 15 13979.0 16 17258.0 17 21883.0 18 18974.0 19 14840.0 20 15022.0 21 15734.0 22 17770.0 23 19951.0 24 22311.0 25 25500.0 26 29212.0 27 34991.0 28 41202.0 29 49624.0 30 60305.0 31 75625.0 32 95476.0 33 125153.0 34 169485.0 35 239785.0 36 344297.0 37 436176.0 38 282437.0 39 27095.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71114783462098 18.31025400101662 14.826591439705842 23.15200672465656 2 15.410729574834855 19.63140680148078 39.650739289476064 25.307124334208297 3 19.30557779650906 23.553595027724448 29.466266974735646 27.674560201030847 4 12.93107961672328 16.005133468870756 36.365444204763286 34.698342709642674 5 14.064204861428864 37.08326587214991 33.73373012241565 15.118799144005576 6 32.957584575377055 35.62910631383779 16.62452805859181 14.788781052193341 7 29.32064685210817 32.52066797668375 19.86858753606031 18.290097635147774 8 27.241479914261184 34.02674519457351 19.165335890301492 19.566439000863813 9 26.5742561535994 16.66341783664883 19.736444348421838 37.025881661329926 10-11 25.439796234492274 26.124067952346753 26.614317096869588 21.821818716291382 12-13 26.45852077311332 23.317497628604865 27.217785276469137 23.006196321812677 14-15 23.10752046140856 24.84099260216844 25.39753499482772 26.653951941595277 16-17 22.598225002447855 28.20820301025679 26.15988179884901 23.033690188446343 18-19 22.813119949239102 26.656202952061182 28.35237636986987 22.178300728829843 20-21 23.370119267560693 25.548192111102264 28.56642213597479 22.515266485362257 22-23 23.143401214002743 25.311574384506947 28.695466233018834 22.84955816847148 24-25 23.138463325375923 25.639283850740934 28.516776114044667 22.705476709838475 26-27 22.49104445334701 25.887255261074287 28.618831738681738 23.002868546896966 28-29 22.647301246566958 26.466367511719984 28.313906744452233 22.572424497260826 30-31 22.719279678264975 25.878366276790167 28.165962500918624 23.23639154402624 32-33 22.428940261457065 25.9393404121335 28.220631846503657 23.411087479905774 34-35 23.273147522643946 25.983929521560434 27.948729910251597 22.794193045544027 36-37 23.311150612243104 25.5493908368129 28.107876222616255 23.031582328327737 38-39 22.82019767016972 25.99584702131905 28.04798342504366 23.13597188346757 40-41 23.07428328567777 25.917228465572943 28.141846542775394 22.86664170597389 42-43 22.86030449460078 25.93347581653766 27.71237458929748 23.49384509956408 44-45 22.88322827779549 25.95656208200295 27.948535658483358 23.211673981718203 46-47 23.550486814625803 26.386965642089866 27.194109506720476 22.86843803656385 48-49 23.222062171545865 26.516692874781544 26.98529220837806 23.275952745294532 50-51 23.105910902950214 26.70495305377141 27.21946030541675 22.969675737861625 52-53 23.55990278812839 26.403605343747678 26.919817813578057 23.11667405454588 54-55 23.113984890798157 26.599232503070276 26.941083090927854 23.345699515203712 56-57 23.252349943804614 26.672711877717788 26.881324455584526 23.19361372289307 58-59 23.324395957285354 26.251800855612622 26.892234600622462 23.531568586479562 60-61 22.812038869715717 26.587167961451406 27.464840001469238 23.13595316736364 62-63 22.92001828294306 26.695934332736364 27.475664901280467 22.908382483040107 64-65 22.680279724586313 26.861236326365876 27.101041104206097 23.35744284484171 66-67 22.75539568978259 26.734463967736716 26.971850564114835 23.53828977836586 68-69 23.06030140585092 26.37087841267914 27.324880103676996 23.243940077792942 70-71 23.134490369821297 26.49436860274702 26.872207259174047 23.498933768257636 72-73 22.972916199711545 26.609326227477 26.931786462697005 23.485971110114452 74-75 22.8115222448642 27.29338021278767 26.663503535316206 23.23159400703192 76-77 23.217017614336104 26.626564018671644 26.77147650578528 23.384941861206972 78-79 23.111389168265937 26.877809719153472 26.79969413712969 23.211106975450896 80-81 23.360978754827574 26.872071202486747 26.537470900376398 23.229479142309287 82-83 23.22833372669601 26.801635890161407 26.8231040340534 23.146926349089174 84-85 22.966587268086897 27.105065019364265 26.656080678166077 23.27226703438276 86-87 23.390546657929256 27.07765935051471 26.376862666683387 23.154931324872653 88-89 23.090317724214906 26.96907491347373 26.20394375913162 23.73666360317975 90-91 22.965128366006628 26.845796895737973 26.547725614264657 23.641349123990747 92-93 23.274324564506223 26.604230787505077 26.753391475425992 23.368053172562707 94-95 22.997835140755445 26.35715030440446 27.042967682843944 23.602046871996155 96-97 23.050110322343627 26.148947263059945 27.14616005649208 23.65478235810435 98-99 22.805761995706025 26.635183092326177 26.639539107223747 23.91951580474405 100-101 22.97444781061986 25.950822361981796 27.229421595068597 23.845308232329742 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3095.0 1 1781.0 2 456.5 3 402.0 4 319.5 5 311.0 6 444.5 7 836.5 8 1406.5 9 1729.0 10 1695.0 11 1624.5 12 1694.5 13 1843.0 14 1994.5 15 2158.5 16 2316.0 17 2431.5 18 2679.5 19 2992.5 20 3386.5 21 3918.5 22 4538.0 23 5530.0 24 6657.0 25 8110.5 26 10411.5 27 13169.0 28 15557.0 29 18211.5 30 21570.5 31 25993.5 32 32976.0 33 36243.5 34 35996.0 35 38055.0 36 42008.0 37 47182.0 38 52436.5 39 55997.0 40 59210.0 41 63882.0 42 66339.0 43 68520.0 44 72377.0 45 77613.5 46 81443.0 47 86989.5 48 95372.5 49 94879.0 50 88246.0 51 84468.5 52 84623.5 53 87033.0 54 88620.5 55 83695.5 56 76572.5 57 70902.5 58 66094.0 59 59050.5 60 48498.0 61 40506.0 62 33534.5 63 24705.0 64 16912.5 65 12654.0 66 9391.5 67 7014.0 68 5450.5 69 4201.5 70 3141.0 71 2081.5 72 1419.0 73 1097.0 74 787.5 75 561.5 76 408.0 77 266.5 78 196.0 79 138.5 80 80.0 81 49.0 82 34.0 83 24.0 84 19.0 85 16.0 86 17.0 87 15.5 88 8.5 89 5.0 90 4.0 91 5.0 92 5.0 93 2.5 94 2.0 95 2.0 96 2.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2442708948419066 2 0.21691993016065067 3 0.2069980713838227 4 0.21389069009161912 5 0.2145492205414086 6 0.2430416380022996 7 0.2442708948419066 8 0.2333831914053874 9 0.22767592750721202 10-11 0.23632462741444699 12-13 0.23718071699917334 14-15 0.22416376510833486 16-17 0.23713681496918734 18-19 0.22756617243224708 20-21 0.21994917022968222 22-23 0.21893942354000503 24-25 0.21358337588171739 26-27 0.221507692294184 28-29 0.23698315786423646 30-31 0.23575390102462948 32-33 0.23619292132448913 34-35 0.2440074826619908 36-37 0.21990526819969627 38-39 0.2183028441052086 40-41 0.21476873069133845 42-43 0.220234533424591 44-45 0.23836607180879438 46-47 0.24266847074741887 48-49 0.24593917198137324 50-51 0.24694891867105043 52-53 0.266046301714945 54-55 0.24618063314629604 56-57 0.21623944869586822 58-59 0.20080788515580172 60-61 0.19810791031166491 62-63 0.2037932231948473 64-65 0.19718596768195962 66-67 0.19769084102679824 68-69 0.20129080748564734 70-71 0.20223470113034558 72-73 0.204166390449728 74-75 0.207876111983542 76-77 0.22161744736914887 78-79 0.2144614164814367 80-81 0.2102248705877911 82-83 0.20473711683954557 84-85 0.20495662698947537 86-87 0.21136632336742617 88-89 0.21029072363277007 90-91 0.22041014154453487 92-93 0.20840293634337356 94-95 0.20511028409442625 96-97 0.21573457535102963 98-99 0.2230003613137068 100-101 0.19887619583641927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2277799.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.40462488856387 #Duplication Level Percentage of deduplicated Percentage of total 1 69.0854370016446 30.677129153205403 2 14.79179844059938 13.13648522364119 3 6.0259671421129735 8.027424316090135 4 3.0993862212734373 5.5050833016172165 5 1.8085431197000477 4.015383941253685 6 1.123296413451424 2.9927733526787788 7 0.7790495161203495 2.421538107305892 8 0.5775627654696072 2.05171663602236 9 0.43422888655771097 1.7353593741036522 >10 2.081814695867593 16.42128092945642 >50 0.12744559571312952 3.860122885224381 >100 0.0570691599945478 4.482281588693333 >500 0.004546445985621936 1.3564358576827358 >1k 0.003656923944956635 2.7863835431590482 >5k 1.976715645922505E-4 0.530601789865849 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6994 0.30705079772183586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.2237247448084752 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4288 0.18825190457981586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3540 0.1554131861503144 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3305 0.14509620910361276 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2523 0.1107648216545885 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2521 0.11067701759461657 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2456 0.10782338564552887 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2453 0.10769167955557096 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2442 0.10720875722572536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.390202998596452E-5 0.0 0.0 0.0 0.0 3 4.390202998596452E-5 0.0 0.0 0.0 0.0 4 4.390202998596452E-5 0.0 0.0 0.0 0.0 5 4.390202998596452E-5 0.0 0.0 0.0 0.0 6 4.390202998596452E-5 0.0 0.0 0.0 0.0 7 4.390202998596452E-5 0.0 0.0 0.0 0.0 8 4.390202998596452E-5 0.0 0.0 0.0 0.0 9 4.390202998596452E-5 0.0 0.0 0.0 0.0 10-11 4.390202998596452E-5 0.0 0.0 0.0 0.0 12-13 4.390202998596452E-5 0.0 0.0 0.0 1.097550749649113E-4 14-15 4.390202998596452E-5 0.0 0.0 0.0 1.3170608995789355E-4 16-17 4.390202998596452E-5 0.0 0.0 0.0 1.3170608995789355E-4 18-19 4.390202998596452E-5 0.0 0.0 0.0 1.3170608995789355E-4 20-21 4.390202998596452E-5 0.0 0.0 0.0 1.536571049508758E-4 22-23 4.390202998596452E-5 0.0 0.0 1.536571049508758E-4 1.7560811994385807E-4 24-25 4.390202998596452E-5 0.0 0.0 3.292652248947339E-4 1.7560811994385807E-4 26-27 4.390202998596452E-5 0.0 0.0 3.5121623988771614E-4 1.7560811994385807E-4 28-29 4.390202998596452E-5 0.0 0.0 6.146284198035033E-4 1.7560811994385807E-4 30-31 4.390202998596452E-5 0.0 0.0 0.0012073058246140243 1.7560811994385807E-4 32-33 4.390202998596452E-5 0.0 0.0 0.0020633954093403324 1.7560811994385807E-4 34-35 4.390202998596452E-5 0.0 0.0 0.0029853380390455877 2.1951014992982262E-4 36-37 4.390202998596452E-5 0.0 0.0 0.004236545893645576 2.1951014992982262E-4 38-39 4.390202998596452E-5 0.0 0.0 0.00572921491316837 2.1951014992982262E-4 40-41 4.390202998596452E-5 0.0 0.0 0.008297483667347293 2.1951014992982262E-4 42-43 4.390202998596452E-5 0.0 0.0 0.011699890991259544 2.4146116492280485E-4 44-45 8.780405997192904E-5 0.0 0.0 0.02056810104842438 2.8536319490876937E-4 46-47 8.780405997192904E-5 0.0 0.0 0.0392264637924593 3.073142099017516E-4 48-49 8.780405997192904E-5 0.0 0.0 0.05542631285728021 3.292652248947339E-4 50-51 8.780405997192904E-5 0.0 0.0 0.07638953217557827 3.5121623988771614E-4 52-53 8.780405997192904E-5 0.0 0.0 0.10360879076687626 3.5121623988771614E-4 54-55 8.780405997192904E-5 0.0 0.0 0.1367109213762935 3.5121623988771614E-4 56-57 8.780405997192904E-5 0.0 0.0 0.17536665877893526 3.5121623988771614E-4 58-59 8.780405997192904E-5 0.0 0.0 0.2234393816135664 3.7316725488069846E-4 60-61 8.780405997192904E-5 0.0 0.0 0.2857583131786431 3.951182698736807E-4 62-63 8.780405997192904E-5 0.0 0.0 0.3571869159658073 4.3902029985964523E-4 64-65 8.780405997192904E-5 0.0 0.0 0.42244728353994365 5.268243598315742E-4 66-67 8.780405997192904E-5 0.0 0.0 0.525924368216862 5.268243598315742E-4 68-69 8.780405997192904E-5 0.0 0.0 0.6514841739767205 5.268243598315742E-4 70-71 8.780405997192904E-5 0.0 0.0 0.7467515790462635 5.268243598315742E-4 72-73 1.097550749649113E-4 0.0 0.0 0.9051720542506165 5.707263898175387E-4 74-75 1.3170608995789355E-4 0.0 0.0 1.0480292598249452 6.146284198035033E-4 76-77 1.3170608995789355E-4 0.0 0.0 1.1819963043271158 6.146284198035033E-4 78-79 1.3170608995789355E-4 0.0 0.0 1.3552556656667247 6.146284198035033E-4 80-81 2.1951014992982262E-4 0.0 2.195101499298226E-5 1.5350564294742424 6.365794347964855E-4 82-83 2.1951014992982262E-4 0.0 4.390202998596452E-5 1.7429983945027634 6.585304497894678E-4 84-85 2.4146116492280485E-4 0.0 4.390202998596452E-5 2.036395660898964 6.585304497894678E-4 86-87 2.634121799157871E-4 0.0 4.390202998596452E-5 2.3971825433236207 7.024324797754323E-4 88-89 2.634121799157871E-4 0.0 4.390202998596452E-5 2.8035616838887014 7.463345097613968E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1295 0.0 27.881952 5 GTATCAA 12705 0.0 27.523975 1 GGTATCA 9160 0.0 26.920303 1 AGGTAAG 1230 0.0 26.649843 2 GTAAGGT 1365 0.0 25.05989 4 TCAACGC 15005 0.0 23.113525 4 ATCAACG 15040 0.0 23.0898 3 TATCAAC 15110 0.0 22.951393 2 CAACGCA 15095 0.0 22.944244 5 GAGTACT 7405 0.0 22.80559 12-13 GTACATG 8850 0.0 22.655685 1 GTACTTT 7505 0.0 22.532621 14-15 AACGCAG 15455 0.0 22.531763 6 GGTAAGG 1560 0.0 21.62143 3 TACATGG 8970 0.0 21.60819 2 AGAGTAC 12065 0.0 20.927256 10-11 AGTACTT 7775 0.0 20.803839 12-13 ACGCAGA 17015 0.0 20.32636 7 CGCAGAG 17120 0.0 20.062067 8 AAGGTAA 1600 0.0 19.895866 1 >>END_MODULE