FastQCFastQC Report
Thu 26 May 2016
SRR937220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277799
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83190.36522098745323883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65950.28953388775743605No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47130.20691026732385082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46220.20291518259512803No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30300.1330231508574725No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27000.11853548096210421No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26420.11598916322291826No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26090.11454039623338143No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26010.11418917999349372No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24760.10870142624524815No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24220.10633071662600607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135150.027.3234271
TAAGGTG14100.025.938475
GTAAGGT12750.025.7046474
GTACATG84350.025.0729331
TACATGG83050.024.7640132
AGGTAAG12250.023.6519242
ATCAACG159800.022.8868853
TCAACGC159800.022.857164
CAACGCA160200.022.8000895
ACATGGG86050.022.7966983
GGTAAGG13200.022.6693633
TATCAAC161950.022.64172
AACGCAG163850.022.3211736
GAGTACT86650.021.4343112-13
GGTATCA97450.021.3122751
AGAGTAC127500.021.14253410-11
AGTACTT89800.020.92047312-13
GTACTTT88300.020.4675914-15
AAGGTAA14150.020.1523191
ACGCAGA181900.019.898657
Produced by FastQC (version 0.11.5)