FastQCFastQC Report
Thu 26 May 2016
SRR937219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262491
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60990.26957013309666206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.20070798071682938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35490.1568625024364738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31400.13878508245999652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.049.016341
GTATCAA71050.041.0130841
ATCAACG87250.032.942473
TCAACGC87800.032.790224
CAACGCA88250.032.6775745
TATCAAC89000.032.457742
AACGCAG92650.031.1721426
ACGCAGA106450.027.1316387
CGCAGAG107650.026.740948
GTACATG74300.023.0324291
GCAGAGT121550.023.0180649
GTATAGG6250.022.8174611
TACATGG72050.022.4207252
GAGTACT70450.022.08055312-13
GTGTAGC13700.021.5128121
AGAGTAC102400.021.15213210-11
GTACTTT73100.021.01925514-15
ACATGGG72350.021.0124973
CAGAGTA111950.020.72667910-11
AGTACTT75250.019.22031212-13
Produced by FastQC (version 0.11.5)