##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937219_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2262491 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.626946140338237 31.0 31.0 34.0 30.0 34.0 2 31.752560783667207 33.0 31.0 34.0 30.0 34.0 3 31.796545047030023 33.0 31.0 34.0 30.0 34.0 4 35.43900727118915 37.0 35.0 37.0 33.0 37.0 5 35.4407946816142 37.0 35.0 37.0 33.0 37.0 6 35.4735550329261 37.0 35.0 37.0 33.0 37.0 7 35.40500978788424 37.0 35.0 37.0 33.0 37.0 8 35.37821763710883 37.0 35.0 37.0 33.0 37.0 9 36.96934043052547 39.0 37.0 39.0 33.0 39.0 10-11 36.847254420017585 39.0 37.0 39.0 33.0 39.0 12-13 36.691679878505596 39.0 37.0 39.0 32.5 39.0 14-15 37.77361722101878 40.0 37.5 41.0 32.5 41.0 16-17 37.66891846199609 40.0 37.0 41.0 32.0 41.0 18-19 37.56508622575736 40.0 37.0 41.0 32.0 41.0 20-21 37.397683128905264 39.5 37.0 41.0 32.0 41.0 22-23 37.484974304870164 40.0 37.0 41.0 32.0 41.0 24-25 37.45114102111346 40.0 37.0 41.0 32.0 41.0 26-27 37.3580688276771 40.0 37.0 41.0 32.0 41.0 28-29 37.20580280761338 39.5 37.0 41.0 31.0 41.0 30-31 36.94078893573499 39.0 36.0 41.0 31.0 41.0 32-33 36.68669157136979 39.0 36.0 40.0 30.0 41.0 34-35 36.67916535358594 39.0 36.0 41.0 30.0 41.0 36-37 36.51595719054794 39.0 35.5 41.0 30.0 41.0 38-39 36.343899710540285 39.0 35.0 40.0 30.0 41.0 40-41 36.08849228571517 39.0 35.0 40.0 29.5 41.0 42-43 35.80309336037138 38.5 35.0 40.0 28.5 41.0 44-45 35.55733613967968 38.0 35.0 40.0 27.5 41.0 46-47 35.18554084856028 38.0 34.5 40.0 26.5 41.0 48-49 34.83111733925129 38.0 34.0 40.0 24.5 41.0 50-51 33.87429938947824 37.5 33.0 39.5 23.5 40.5 52-53 33.98628414433472 37.5 33.0 39.5 24.0 40.0 54-55 34.248789055956465 38.0 33.5 40.0 23.5 41.0 56-57 33.9263106460976 38.0 33.0 40.0 22.5 41.0 58-59 33.59640502437358 37.5 33.0 40.0 22.0 41.0 60-61 33.21626605365502 37.0 32.0 40.0 20.5 41.0 62-63 32.771188261080376 36.5 31.5 39.0 18.5 41.0 64-65 32.28064752522772 36.0 31.0 39.0 15.5 40.0 66-67 31.791363147963903 35.0 31.0 39.0 12.0 40.0 68-69 31.240984605021634 35.0 30.0 38.0 8.0 40.0 70-71 30.707060050183625 35.0 29.5 37.5 7.0 40.0 72-73 30.15285298372458 34.0 29.0 37.0 2.0 39.0 74-75 29.53256720137229 34.0 28.5 36.0 2.0 39.0 76-77 28.943522648266885 34.0 27.5 36.0 2.0 38.5 78-79 28.320542490555763 33.5 26.5 35.0 2.0 37.5 80-81 27.665227618585 33.0 26.0 35.0 2.0 37.0 82-83 27.01160247709273 32.5 25.0 35.0 2.0 36.0 84-85 26.33451889974369 32.0 23.5 35.0 2.0 36.0 86-87 25.629560736374202 31.5 21.0 34.5 2.0 35.5 88-89 24.935774109156675 31.0 19.0 34.0 2.0 35.0 90-91 24.24657446151167 31.0 14.0 34.0 2.0 35.0 92-93 23.440337884216998 30.5 4.5 34.0 2.0 35.0 94-95 22.52927746452914 30.0 2.0 34.0 2.0 35.0 96-97 21.514334863652493 29.0 2.0 34.0 2.0 35.0 98-99 20.177651535409424 29.0 2.0 34.0 2.0 35.0 100-101 17.180899062139915 22.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12896521525017945 1101 2 0.20734329117331285 1101 3 0.16425337714226984 1101 4 0.002860362356194912 1101 5 -0.002236763091367777 1101 6 -0.0350642042278011 1101 7 0.04104186365902507 1101 8 0.05952188487034249 1101 9 0.08987023549869377 1101 10-11 0.24220008662759795 1101 12-13 0.24947243895447713 1101 14-15 0.307444161196166 1101 16-17 0.4492261734078866 1101 18-19 0.35463268947531645 1101 20-21 0.4552414746894442 1101 22-23 0.25047220333408404 1101 24-25 0.3740014079684073 1101 26-27 0.22329628795947798 1101 28-29 0.3343102444252182 1101 30-31 0.26967299259884925 1101 32-33 0.09847342695000805 1101 34-35 0.24679877474082446 1101 36-37 0.29999434455444884 1101 38-39 0.37287572734876306 1101 40-41 0.499008157051108 1101 42-43 0.40625265265369137 1101 44-45 0.535935827000614 1101 46-47 0.7797306965519937 1101 48-49 0.7558292994009577 1101 50-51 0.699869077051396 1101 52-53 0.801199369332771 1101 54-55 0.7802606705486994 1101 56-57 0.7640825133247588 1101 58-59 0.7373507423631693 1101 60-61 0.9443917204887455 1101 62-63 0.8971580938918962 1101 64-65 0.7376833722413352 1101 66-67 0.949903908415525 1101 68-69 0.9905104662327204 1101 70-71 0.9696450813609552 1101 72-73 0.8319028144280338 1101 74-75 1.034480348476464 1101 76-77 0.8468295993205537 1101 78-79 1.0636890504976009 1101 80-81 1.1243745374482543 1101 82-83 1.031934017637191 1101 84-85 1.3691532198553773 1101 86-87 1.3949209373657858 1101 88-89 1.416823688240978 1101 90-91 1.3466621683094822 1101 92-93 1.6213776276070249 1101 94-95 1.7786101537612566 1101 96-97 1.7793938504066027 1101 98-99 1.86852654312424 1101 100-101 1.7283823845173707 1102 1 0.29175985960494444 1102 2 0.19950751613050244 1102 3 0.08958272313248727 1102 4 0.14005414705222563 1102 5 0.15907859371096578 1102 6 0.14065761221389295 1102 7 0.02470278641082757 1102 8 -0.03964079101481133 1102 9 0.18144416265194252 1102 10-11 0.11192635743625345 1102 12-13 0.1917682049737408 1102 14-15 0.4169124267467552 1102 16-17 0.4795062711061675 1102 18-19 0.563397907740196 1102 20-21 0.5591644749558498 1102 22-23 0.41712616288599236 1102 24-25 0.4800866508820931 1102 26-27 0.4978707864844054 1102 28-29 0.49652454737420015 1102 30-31 0.5526828027904358 1102 32-33 0.5131696088058604 1102 34-35 0.49801852321171225 1102 36-37 0.6648576772927939 1102 38-39 0.5685518699351491 1102 40-41 0.6127147985068575 1102 42-43 0.6933532867237702 1102 44-45 0.8048365240381798 1102 46-47 0.818607919811221 1102 48-49 0.7872820096491395 1102 50-51 0.7119044787741231 1102 52-53 0.6492728062903907 1102 54-55 0.7320010220364708 1102 56-57 0.7651552928012961 1102 58-59 0.8485394017565753 1102 60-61 0.7430674298372608 1102 62-63 0.8676644619503335 1102 64-65 0.762611143506092 1102 66-67 0.8696327355966709 1102 68-69 0.9650105160147149 1102 70-71 0.9633929118079827 1102 72-73 1.1024186007009398 1102 74-75 1.0852874894563698 1102 76-77 1.1455255802544997 1102 78-79 1.1637534531451088 1102 80-81 1.253174899107691 1102 82-83 1.3103630774033839 1102 84-85 1.4394596526752537 1102 86-87 1.5639022722586553 1102 88-89 1.5635353410838029 1102 90-91 1.5276344150738765 1102 92-93 1.6511184652540507 1102 94-95 1.5611746169544247 1102 96-97 1.5144706895820725 1102 98-99 1.8762980052168245 1102 100-101 1.9181811127893234 1103 1 0.18963481228504264 1103 2 0.332324322299808 1103 3 0.21518123270669065 1103 4 0.23477208322546517 1103 5 0.26553112173318283 1103 6 0.2869278737359977 1103 7 0.3046758873131381 1103 8 0.23944144004769186 1103 9 0.10856379201734967 1103 10-11 0.16576910560879554 1103 12-13 0.24086724332169496 1103 14-15 0.44135498201221424 1103 16-17 0.3831546705075439 1103 18-19 0.3574209505564525 1103 20-21 0.3864258547530568 1103 22-23 0.2702986659065516 1103 24-25 0.37219243539725255 1103 26-27 0.2259926211665686 1103 28-29 0.10589551588122958 1103 30-31 0.14721759814987934 1103 32-33 0.36180693511740003 1103 34-35 0.5562293053597216 1103 36-37 0.4173640276935373 1103 38-39 0.48753573140665196 1103 40-41 0.7738123040154932 1103 42-43 0.5852335169101011 1103 44-45 0.7929852950228735 1103 46-47 0.8359697433691338 1103 48-49 0.8015620634845462 1103 50-51 0.8297110861672721 1103 52-53 0.7381621395703917 1103 54-55 0.8886670593212997 1103 56-57 0.7879981330558223 1103 58-59 1.0390038948546092 1103 60-61 0.9804169944011463 1103 62-63 0.965416173605405 1103 64-65 0.8707590959843543 1103 66-67 1.2170987606038999 1103 68-69 1.2518292140500193 1103 70-71 1.3389838408964927 1103 72-73 1.2382674151222801 1103 74-75 1.3169623861431816 1103 76-77 1.3276400055932278 1103 78-79 1.4030646427790963 1103 80-81 1.246598478975784 1103 82-83 1.5438831480181676 1103 84-85 1.5294548067451395 1103 86-87 1.6923344882997071 1103 88-89 1.6449507805333923 1103 90-91 1.7601054952884354 1103 92-93 1.849582928588216 1103 94-95 1.9552348646084283 1103 96-97 1.9175172077208238 1103 98-99 2.1607681478694722 1103 100-101 1.8206934624701496 1104 1 0.11242226118969612 1104 2 0.16550115985375058 1104 3 0.15129061188018866 1104 4 0.1657273307861118 1104 5 0.15570511788971686 1104 6 0.1448251172879793 1104 7 0.20292514216570368 1104 8 0.12194609142346735 1104 9 0.33758585304668287 1104 10-11 0.24314819039241087 1104 12-13 0.2393870819580073 1104 14-15 0.46422506680673337 1104 16-17 0.360661628690373 1104 18-19 0.313081977447915 1104 20-21 0.320541227227352 1104 22-23 0.3137245717035597 1104 24-25 0.30066755467151296 1104 26-27 0.3336479511145427 1104 28-29 0.40190790399918086 1104 30-31 0.3501912856359368 1104 32-33 0.34432510421046914 1104 34-35 0.5412607756380439 1104 36-37 0.5117229523409605 1104 38-39 0.690164497223769 1104 40-41 0.6274681964687687 1104 42-43 0.5653352267388172 1104 44-45 0.5367393735208523 1104 46-47 0.6677739685830986 1104 48-49 0.7518312037213946 1104 50-51 0.5815526600502068 1104 52-53 0.6548039982622598 1104 54-55 0.9329936045426521 1104 56-57 1.0016923072255821 1104 58-59 1.1548960460320288 1104 60-61 0.9793433205475282 1104 62-63 0.9974480012332876 1104 64-65 0.9877141183134484 1104 66-67 1.0239540022947615 1104 68-69 1.035556826884232 1104 70-71 1.1402251191486634 1104 72-73 1.2209124442637638 1104 74-75 1.3245228435123764 1104 76-77 1.3501273114624581 1104 78-79 1.4606615903370432 1104 80-81 1.4271602883079026 1104 82-83 1.6056441069768859 1104 84-85 1.3802688237958165 1104 86-87 1.4055745665915715 1104 88-89 1.510777876501347 1104 90-91 1.9385806623622628 1104 92-93 2.0686355297163246 1104 94-95 2.2621587464761994 1104 96-97 2.2923460135748606 1104 98-99 2.282626258490289 1104 100-101 1.793737470765862 1105 1 0.24708474232777178 1105 2 0.16160044864962586 1105 3 0.2340545902213833 1105 4 0.21570858543747562 1105 5 0.13843962593156078 1105 6 0.1780411289865711 1105 7 0.24975065001543584 1105 8 0.10753865893936165 1105 9 0.19606910253888543 1105 10-11 0.2336233150349969 1105 12-13 0.2911947490853848 1105 14-15 0.36969758948301035 1105 16-17 0.3865633172639278 1105 18-19 0.40743832186245754 1105 20-21 0.42957070312233014 1105 22-23 0.5383644465107409 1105 24-25 0.43657795377023945 1105 26-27 0.45318069765318114 1105 28-29 0.32689638633471674 1105 30-31 0.3839116497993018 1105 32-33 0.4495772524647279 1105 34-35 0.5510296203090519 1105 36-37 0.5297235230948587 1105 38-39 0.6047748644274265 1105 40-41 0.5929868271070546 1105 42-43 0.49148274983476625 1105 44-45 0.58329880933249 1105 46-47 0.6962540285682124 1105 48-49 0.6923414875502161 1105 50-51 0.5182424366541412 1105 52-53 0.5081881398838348 1105 54-55 0.6289527228957468 1105 56-57 0.6747851778379186 1105 58-59 0.8669938836954358 1105 60-61 0.9760742623101137 1105 62-63 0.7912469279921268 1105 64-65 0.8478355807117666 1105 66-67 0.8847599030693836 1105 68-69 0.6877636486989758 1105 70-71 0.7811650538290529 1105 72-73 0.8915977965923823 1105 74-75 0.9905538470133735 1105 76-77 0.9966293921028999 1105 78-79 1.0813332333808425 1105 80-81 1.0820436847785544 1105 82-83 1.3195097895593761 1105 84-85 1.3986104479823531 1105 86-87 1.3612405114224266 1105 88-89 1.4355054161230463 1105 90-91 1.4945976394929374 1105 92-93 1.6110985939186797 1105 94-95 1.7578132762290757 1105 96-97 1.8132548179200505 1105 98-99 1.7517482860468832 1105 100-101 1.3223953784119153 1106 1 0.014399583731275811 1106 2 -0.5342885376832136 1106 3 -0.11166016115425847 1106 4 0.09287061230613602 1106 5 -0.004388025039730792 1106 6 0.061205918779272395 1106 7 0.10207701653748558 1106 8 0.08564985887106502 1106 9 0.10196398724889377 1106 10-11 -0.39327580719335486 1106 12-13 -0.2722712071890143 1106 14-15 -0.3394763272722585 1106 16-17 -0.2017289861299716 1106 18-19 0.03513605331555425 1106 20-21 -0.08077202363534752 1106 22-23 0.032919701111083555 1106 24-25 0.02353904776398963 1106 26-27 0.06775492795376437 1106 28-29 0.1607489967546627 1106 30-31 0.13313709974458732 1106 32-33 0.11369222425341263 1106 34-35 0.026408164185994565 1106 36-37 0.10971075621269222 1106 38-39 0.4188894481649825 1106 40-41 0.38692915494817726 1106 42-43 0.26625011318255787 1106 44-45 0.21584815003362223 1106 46-47 0.21008378277662842 1106 48-49 0.42315973216212655 1106 50-51 0.19996852688379363 1106 52-53 0.2375927142305514 1106 54-55 0.4339360755022881 1106 56-57 0.30635856994805977 1106 58-59 0.22379564389536455 1106 60-61 0.2445800326304095 1106 62-63 0.30479844954060553 1106 64-65 0.4971004178467098 1106 66-67 0.5969988416719367 1106 68-69 0.41761661612596157 1106 70-71 0.47905541386288775 1106 72-73 0.44225961779394396 1106 74-75 0.24749239523648825 1106 76-77 0.5664374620141892 1106 78-79 0.5928998734013291 1106 80-81 0.6303440208308331 1106 82-83 0.3500971437491671 1106 84-85 0.352746490947375 1106 86-87 0.46248285711389414 1106 88-89 0.589654070749777 1106 90-91 0.6369307192995279 1106 92-93 0.7395751439889153 1106 94-95 0.7257214478097644 1106 96-97 0.5068054081458193 1106 98-99 0.5322624925987398 1106 100-101 0.18754101400085688 1107 1 0.0021894940405822183 1107 2 0.12814164026350383 1107 3 0.13104468567471983 1107 4 0.059465203747606665 1107 5 -0.026876125927174144 1107 6 0.0342104230210154 1107 7 0.0443897720393025 1107 8 -0.04870921291144015 1107 9 0.10005673962911033 1107 10-11 0.09255069042367126 1107 12-13 -0.003217058541160611 1107 14-15 0.0630171654621492 1107 16-17 0.2467368994020589 1107 18-19 0.19563237913445874 1107 20-21 0.19518766994377756 1107 22-23 0.16903112315554125 1107 24-25 0.14036633996898473 1107 26-27 0.14547221386828113 1107 28-29 0.18124136539933744 1107 30-31 0.09650131270046813 1107 32-33 0.038825562106225675 1107 34-35 0.16833395219181568 1107 36-37 0.32155535908425037 1107 38-39 0.31254078485429204 1107 40-41 0.16666176190618387 1107 42-43 0.15074595184336914 1107 44-45 0.19017736170460608 1107 46-47 0.3845687589153428 1107 48-49 0.42794696704327606 1107 50-51 0.28104744019015016 1107 52-53 0.5397182370584019 1107 54-55 0.5621071357884944 1107 56-57 0.5526701997835772 1107 58-59 0.4548717588016089 1107 60-61 0.5500648180049481 1107 62-63 0.7432186699126433 1107 64-65 0.5811275047831401 1107 66-67 0.6929293767102642 1107 68-69 0.7314577789360897 1107 70-71 0.8984289742633997 1107 72-73 0.9536879290895506 1107 74-75 1.004918994384198 1107 76-77 0.9444370710264565 1107 78-79 0.9965249445437543 1107 80-81 0.9745756116937017 1107 82-83 0.9563543663804168 1107 84-85 0.9178232853524655 1107 86-87 0.8724234443207095 1107 88-89 0.9180877076766762 1107 90-91 1.1596275177721544 1107 92-93 1.2638287988571086 1107 94-95 1.3763002042480572 1107 96-97 1.3389364804769706 1107 98-99 1.5633604034945279 1107 100-101 1.3204125981790966 1108 1 0.0624900696541566 1108 2 0.0881536227837536 1108 3 0.10739416715455263 1108 4 0.09530881881719466 1108 5 0.11154959951584686 1108 6 0.11358867443991016 1108 7 0.05346603249571302 1108 8 0.03574588351447261 1108 9 0.0909659037639372 1108 10-11 0.18402691467170484 1108 12-13 0.23426803522666972 1108 14-15 0.2255608773047797 1108 16-17 0.24435132690877737 1108 18-19 0.19925992987894148 1108 20-21 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2.4831678370996677 2315 92-93 2.4264821840670656 2315 94-95 2.8461241447027135 2315 96-97 2.6768491403684465 2315 98-99 3.1452319958379533 2315 100-101 3.816687761161049 2316 1 -0.26254545049208033 2316 2 -0.24744439433190024 2316 3 -0.45158986699084025 2316 4 -0.36071671981922293 2316 5 -0.16108194237401108 2316 6 -0.19808480546721086 2316 7 -0.1046860209639604 2316 8 -0.02463139058857422 2316 9 -0.32443304554382735 2316 10-11 -0.30277810103643077 2316 12-13 -0.2344172222907943 2316 14-15 -0.37242535126273424 2316 16-17 -0.3927214543524471 2316 18-19 -0.1945623434059982 2316 20-21 -0.26398483275356455 2316 22-23 -0.4109864847570961 2316 24-25 -0.3695121685052385 2316 26-27 -0.39889891424475366 2316 28-29 -0.14817753838195102 2316 30-31 -0.24377280559855308 2316 32-33 -0.09062407764018587 2316 34-35 0.07022347910366022 2316 36-37 -0.06177335251938132 2316 38-39 -0.05272873731004779 2316 40-41 -0.13033234407213 2316 42-43 -0.0667222997255763 2316 44-45 -0.18025409138915904 2316 46-47 0.005667145156508013 2316 48-49 -0.012457949131558621 2316 50-51 0.05029822416567953 2316 52-53 0.13595899378180576 2316 54-55 0.16687399658243862 2316 56-57 0.154448914875438 2316 58-59 -0.038481945244164706 2316 60-61 -0.06071519102497547 2316 62-63 0.2851426579622611 2316 64-65 0.409107237035812 2316 66-67 0.5169201247679318 2316 68-69 0.6352469683885431 2316 70-71 0.5637543520788348 2316 72-73 0.6363920709637831 2316 74-75 0.640558058940254 2316 76-77 0.7790818567281086 2316 78-79 0.8720461506046 2316 80-81 1.1267803961492433 2316 82-83 1.1794963761922332 2316 84-85 1.2786496945010342 2316 86-87 1.43089746617769 2316 88-89 1.2388523860196479 2316 90-91 1.3902728545712009 2316 92-93 1.6352352029071433 2316 94-95 1.7151329571594154 2316 96-97 1.775368427627786 2316 98-99 2.065593861687688 2316 100-101 2.640852365998608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9067.0 3 1734.0 4 1665.0 5 2093.0 6 2283.0 7 2896.0 8 3709.0 9 4510.0 10 5764.0 11 7483.0 12 9644.0 13 11802.0 14 13985.0 15 16796.0 16 19781.0 17 23882.0 18 21965.0 19 19329.0 20 20433.0 21 22363.0 22 24663.0 23 28285.0 24 31775.0 25 36264.0 26 42009.0 27 49134.0 28 57226.0 29 67323.0 30 79726.0 31 96268.0 32 118606.0 33 147664.0 34 187064.0 35 240325.0 36 304454.0 37 331039.0 38 185284.0 39 14196.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52967363325308 19.04826284520242 15.179619842381886 23.24244367916261 2 14.910598090862806 19.705241553310813 40.98410558876584 24.40005476706054 3 19.062939492198474 23.84430444696318 29.58957244249326 27.503183618345084 4 12.557075905803764 16.515889533392148 37.56732242940755 33.35971213139655 5 13.803736164405414 37.18967946616095 34.4841505454481 14.522433823985537 6 32.216525670363666 36.409648523636264 17.347993759485025 14.025832046515049 7 28.660307937101383 32.45840262169132 20.4940566314385 18.387232809768793 8 26.299912424434513 35.24276416303319 19.564035811628813 18.893287600903484 9 25.812282284956346 17.15865891741769 20.118586723408253 36.91047207421771 10-11 24.730254056407013 26.708475894159594 27.514075170214582 21.047194879218814 12-13 25.676513821783654 23.754936351304295 28.02312476243048 22.545425064481574 14-15 22.461980961299552 25.405384693246308 25.978596689324192 26.154037656129947 16-17 22.11552921451961 28.70746943336135 26.778557008815906 22.398444343303133 18-19 22.187813218756077 27.26236122042318 28.908715403929563 21.641110156891177 20-21 22.565161957858468 26.41172891987714 29.26367812573983 21.75943099652456 22-23 22.254597235955828 26.260922137932024 29.214071135215818 22.270409490896327 24-25 22.157000964920265 26.492963301803453 29.215601987775607 22.134433745500676 26-27 21.94815585973253 26.799854636388336 28.9842507252125 22.267738778666626 28-29 21.982398417695936 27.07308078632361 28.918666603173687 22.02585419280676 30-31 22.194705728077572 26.643738880185996 28.837687043245886 22.323868348490542 32-33 21.88848759124993 26.703564589157697 28.798209798090635 22.609738021501737 34-35 22.381894664473027 26.902868478103905 28.581300715768432 22.13393614165464 36-37 22.40996931761254 26.51096473452227 28.708961144058392 22.370104803806797 38-39 22.022334814053703 26.85401543905647 28.669256081951893 22.45439366493793 40-41 22.428061178169127 26.65594919386447 28.69720703544516 22.21878259252124 42-43 22.22481828254443 26.639476687390612 28.57127038224171 22.564434647823255 44-45 22.265123519194745 26.944460377102768 28.53056328012662 22.259852823575866 46-47 22.622131198917053 27.089933894902927 27.934436642692944 22.353498263487072 48-49 22.406753608037352 27.237758365596733 27.826960389109885 22.528527637256033 50-51 22.475339354538022 27.401443254985903 27.785637847420432 22.337579543055643 52-53 22.73963747357349 27.159884739095595 27.719396809833018 22.381080977497895 54-55 22.430701631788885 27.258269966672284 27.849960386377344 22.461068015161487 56-57 22.524548024488013 27.404213876393374 27.74082884210741 22.330409257011205 58-59 22.378677450696454 27.149144832460497 27.83164921435828 22.640528502484774 60-61 22.224670169272954 27.33983636002919 27.98798385211113 22.44750961858672 62-63 22.261665082653597 27.32209968387646 28.06922830957609 22.347006923893854 64-65 22.109649388900525 27.30540094227868 28.003075978802052 22.581873690018746 66-67 22.14053434529659 27.418333404928934 27.786836712867235 22.654295536907238 68-69 22.46449964028546 27.144442271961022 27.95262086751766 22.438437220235862 70-71 22.348549182446035 27.17825247327762 27.766210190455165 22.706988153821182 72-73 22.335948682700074 27.230428281912104 27.77069663128357 22.66292640410425 74-75 22.292118280162796 27.518887769105177 27.583594585443443 22.60539936528858 76-77 22.408489623947982 27.286732327070045 27.61978121713224 22.684996831849734 78-79 22.423828070497155 27.396934740654217 27.615549628968168 22.563687559880456 80-81 22.528519320589872 27.377661952736087 27.445293816600724 22.648524910073316 82-83 22.500311117271945 27.354471297715538 27.57415773579257 22.57105984921995 84-85 22.46268256857821 27.42233957899463 27.502157633683616 22.612820218743536 86-87 22.56456174798633 27.419761959483424 27.381169052872945 22.6345072396573 88-89 22.535767359866284 27.44637359527485 27.23578522998309 22.782073814875776 90-91 22.46156482943573 27.410567050658543 27.346549952269505 22.781318167636226 92-93 22.56168301929076 27.14548886141492 27.533018039593966 22.759810079700358 94-95 22.44900218734458 26.999201501867372 27.555625824572218 22.99617048621583 96-97 22.61885437494782 26.785647454364632 27.639193725085153 22.956304445602395 98-99 22.475268859591523 27.001296213166277 27.29995896321549 23.223475964026708 100-101 23.044334499797618 26.028339679359604 27.937616746069494 22.989709074773284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3180.0 1 1764.5 2 348.5 3 329.5 4 283.5 5 251.0 6 370.5 7 796.0 8 1317.5 9 1577.0 10 1472.0 11 1337.0 12 1404.0 13 1542.0 14 1701.5 15 1855.5 16 2021.0 17 2224.0 18 2470.0 19 2741.0 20 3069.0 21 3682.0 22 4543.5 23 5694.0 24 7092.5 25 8985.5 26 11613.5 27 14967.5 28 18180.5 29 21380.0 30 25226.0 31 30772.0 32 38328.5 33 42842.5 34 45485.0 35 49223.0 36 53910.5 37 59360.5 38 63659.0 39 67242.0 40 70660.5 41 74518.5 42 76764.5 43 78010.0 44 80533.5 45 84036.5 46 86086.5 47 86949.0 48 87063.5 49 85127.0 50 82844.0 51 80640.5 52 79029.0 53 77859.5 54 76033.5 55 70809.0 56 64202.5 57 58050.0 58 52548.5 59 46220.5 60 37488.5 61 30464.5 62 25036.0 63 18747.0 64 13221.0 65 9746.5 66 7130.5 67 5322.0 68 4032.5 69 2996.5 70 2272.5 71 1580.5 72 1083.5 73 807.0 74 570.0 75 416.5 76 299.0 77 192.0 78 115.0 79 73.5 80 57.0 81 44.5 82 34.0 83 21.0 84 14.0 85 16.0 86 14.0 87 9.0 88 9.0 89 7.0 90 6.5 91 7.0 92 4.5 93 4.0 94 2.5 95 1.5 96 1.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28870833077347047 2 0.2501667409947708 3 0.24861977351512116 4 0.24875237072766254 5 0.24888496794020393 6 0.22033236817295626 7 0.22174673844006454 8 0.22152574308582887 9 0.23160313123897508 10-11 0.23927167003095262 12-13 0.23235451544337632 14-15 0.2226086203215836 16-17 0.2176141253158576 18-19 0.20702844784796934 20-21 0.21169144982234184 22-23 0.20987928791760938 24-25 0.2117798479640361 26-27 0.20793452880033558 28-29 0.2220340324005709 30-31 0.2324429135850706 32-33 0.22506166875359948 34-35 0.22351470127394982 36-37 0.21410029918351056 38-39 0.2024759435507147 40-41 0.19871902252870838 42-43 0.21200084331827176 44-45 0.2088185102172782 46-47 0.2187633011578831 48-49 0.22236552543192437 50-51 0.23432137409607373 52-53 0.24417776689498435 54-55 0.22366939802191477 56-57 0.2019897537713962 58-59 0.1916913702640143 60-61 0.19284054610603976 62-63 0.19947040673310965 64-65 0.19217756004333278 66-67 0.1961554764195747 68-69 0.19670796480516384 70-71 0.19913891370175615 72-73 0.2006195825751351 74-75 0.2039566124240936 76-77 0.21500638013587678 78-79 0.21516107688384173 80-81 0.20682955202915723 82-83 0.19889581881209692 84-85 0.18696206968337112 86-87 0.19023280092605893 88-89 0.20028808954378163 90-91 0.19891791834752048 92-93 0.20316102914884523 94-95 0.19898421695379118 96-97 0.20665275574576872 98-99 0.21040967676777497 100-101 0.19381292566467667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2262491.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.00511905399791 #Duplication Level Percentage of deduplicated Percentage of total 1 75.4670232521039 43.02006645137011 2 13.77851426861465 15.708916925391742 3 4.607355860567232 7.879286080673101 4 2.0954049191905293 4.777952275391559 5 1.1725876938905266 3.342175054574117 6 0.6728993414355925 2.3015224241935646 7 0.4482261658909205 1.7885830164810257 8 0.31584876917376165 1.440399735184719 9 0.22938375819829335 1.176844360063246 >10 1.1229690509595678 11.18221540706288 >50 0.06176583515020664 2.3930454427129733 >100 0.02437662698722038 2.5049329426907616 >500 0.002403791339686284 0.9535409331848781 >1k 0.0011631248417832815 1.2609266020779129 >5k 7.754165611888544E-5 0.269592348947388 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6099 0.26957013309666206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4541 0.20070798071682938 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3549 0.1568625024364738 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3140 0.13878508245999652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 4.419907084713265E-5 0.0 0.0 4.419907084713265E-5 4.419907084713265E-5 14-15 4.419907084713265E-5 0.0 0.0 4.419907084713265E-5 6.629860627069898E-5 16-17 4.419907084713265E-5 0.0 0.0 8.83981416942653E-5 8.83981416942653E-5 18-19 8.83981416942653E-5 0.0 0.0 1.3259721254139795E-4 8.83981416942653E-5 20-21 8.83981416942653E-5 0.0 0.0 1.5469674796496428E-4 8.83981416942653E-5 22-23 8.83981416942653E-5 0.0 0.0 2.651944250827959E-4 8.83981416942653E-5 24-25 1.3259721254139795E-4 0.0 0.0 3.535925667770612E-4 1.3259721254139795E-4 26-27 1.3259721254139795E-4 0.0 0.0 5.524883855891581E-4 1.3259721254139795E-4 28-29 1.3259721254139795E-4 0.0 0.0 0.0010386781649076174 1.767962833885306E-4 30-31 1.3259721254139795E-4 0.0 0.0 0.0024751479674394285 1.767962833885306E-4 32-33 1.3259721254139795E-4 0.0 0.0 0.005104992682843821 2.2099535423566327E-4 34-35 1.5469674796496428E-4 0.0 0.0 0.008928212311120795 3.3149303135349487E-4 36-37 1.767962833885306E-4 0.0 0.0 0.012839830081092036 3.535925667770612E-4 38-39 1.767962833885306E-4 0.0 0.0 0.016795646921910407 3.535925667770612E-4 40-41 1.767962833885306E-4 0.0 0.0 0.021038757723235144 3.535925667770612E-4 42-43 1.9889581881209694E-4 0.0 0.0 0.02665203972082099 3.535925667770612E-4 44-45 2.2099535423566327E-4 0.0 0.0 0.03299460638738452 3.756921022006276E-4 46-47 2.430948896592296E-4 0.0 0.0 0.04247530708409448 3.977916376241939E-4 48-49 2.651944250827959E-4 0.0 0.0 0.05498364413383302 3.977916376241939E-4 50-51 2.651944250827959E-4 0.0 0.0 0.06828756445881995 4.4199070847132654E-4 52-53 2.651944250827959E-4 0.0 0.0 0.0904312989532334 5.303888501655918E-4 54-55 2.651944250827959E-4 0.0 0.0 0.12833200220464966 5.303888501655918E-4 56-57 2.651944250827959E-4 0.0 0.0 0.1853930026682979 5.524883855891581E-4 58-59 2.651944250827959E-4 0.0 0.0 0.23843188768485707 5.745879210127245E-4 60-61 2.651944250827959E-4 0.0 0.0 0.29100668245752137 5.745879210127245E-4 62-63 2.651944250827959E-4 0.0 0.0 0.35536052961094655 5.745879210127245E-4 64-65 2.651944250827959E-4 0.0 0.0 0.4289519825714224 5.745879210127245E-4 66-67 3.0939349592992856E-4 0.0 0.0 0.5295490678194963 5.745879210127245E-4 68-69 3.535925667770612E-4 0.0 0.0 0.6533064661914677 5.745879210127245E-4 70-71 3.535925667770612E-4 0.0 0.0 0.7808649846562925 5.745879210127245E-4 72-73 3.977916376241939E-4 0.0 0.0 0.9173738149676618 6.187869918598571E-4 74-75 4.4199070847132654E-4 0.0 0.0 1.069728012177728 6.629860627069897E-4 76-77 4.4199070847132654E-4 0.0 0.0 1.2362038125234531 7.071851335541224E-4 78-79 4.4199070847132654E-4 0.0 0.0 1.4185028802324517 7.292846689776887E-4 80-81 4.4199070847132654E-4 0.0 0.0 1.6114539240156094 7.51384204401255E-4 82-83 4.4199070847132654E-4 0.0 0.0 1.8292448456148556 7.51384204401255E-4 84-85 4.4199070847132654E-4 2.2099535423566326E-5 0.0 2.0784834061218365 7.734837398248214E-4 86-87 4.4199070847132654E-4 4.419907084713265E-5 0.0 2.3486723262103584 7.955832752483878E-4 88-89 4.4199070847132654E-4 4.419907084713265E-5 0.0 2.6560326648813186 7.955832752483878E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4015 0.0 49.01634 1 GTATCAA 7105 0.0 41.013084 1 ATCAACG 8725 0.0 32.94247 3 TCAACGC 8780 0.0 32.79022 4 CAACGCA 8825 0.0 32.677574 5 TATCAAC 8900 0.0 32.45774 2 AACGCAG 9265 0.0 31.172142 6 ACGCAGA 10645 0.0 27.131638 7 CGCAGAG 10765 0.0 26.74094 8 GTACATG 7430 0.0 23.032429 1 GCAGAGT 12155 0.0 23.018064 9 GTATAGG 625 0.0 22.817461 1 TACATGG 7205 0.0 22.420725 2 GAGTACT 7045 0.0 22.080553 12-13 GTGTAGC 1370 0.0 21.512812 1 AGAGTAC 10240 0.0 21.152132 10-11 GTACTTT 7310 0.0 21.019255 14-15 ACATGGG 7235 0.0 21.012497 3 CAGAGTA 11195 0.0 20.726679 10-11 AGTACTT 7525 0.0 19.220312 12-13 >>END_MODULE