FastQCFastQC Report
Thu 26 May 2016
SRR937219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262491
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68040.3007304780438905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54630.24145952403788568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.17122720046179188No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22700.10033189082299113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.045.942481
GTATCAA77000.039.246861
ATCAACG94450.031.6820963
TCAACGC95850.031.2193414
TATCAAC97300.030.75412
CAACGCA97900.030.565625
AACGCAG101750.029.4557616
ACGCAGA115050.025.8894867
CGCAGAG116200.025.5923828
GCAGAGT130150.022.4477839
GAGTACT77150.021.70529612-13
TATACCG2858.367351E-1121.6657525
GTACATG69800.020.966881
AGAGTAC111750.020.87259510-11
GTACTTT80400.020.70652814-15
TACATGG69300.020.6989842
ACATGGG67950.019.8519953
TAAGGTG12950.019.8061165
AGTACTT82950.019.78673712-13
CAGAGTA122850.019.56671310-11
Produced by FastQC (version 0.11.5)