##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937219_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2262491 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0382755113722 31.0 31.0 34.0 30.0 34.0 2 32.24904762052092 33.0 31.0 34.0 30.0 34.0 3 32.32398095727232 34.0 31.0 34.0 30.0 34.0 4 35.99992839750523 37.0 35.0 37.0 35.0 37.0 5 35.91181799176218 37.0 35.0 37.0 35.0 37.0 6 35.94651956626568 37.0 35.0 37.0 35.0 37.0 7 35.88255378695429 37.0 35.0 37.0 35.0 37.0 8 35.84907696870396 37.0 35.0 37.0 35.0 37.0 9 37.524578882302734 39.0 37.0 39.0 35.0 39.0 10-11 37.430902708563266 39.0 37.0 39.0 34.5 39.0 12-13 37.31530777359998 39.0 37.0 39.0 34.0 39.0 14-15 38.50916865525653 40.0 38.0 41.0 34.0 41.0 16-17 38.444857902197185 40.0 38.0 41.0 34.0 41.0 18-19 38.399833855692684 40.0 38.0 41.0 34.0 41.0 20-21 38.26928748003859 40.0 38.0 41.0 34.0 41.0 22-23 38.21717500754699 40.0 38.0 41.0 33.5 41.0 24-25 38.253164100984264 40.0 38.0 41.0 34.0 41.0 26-27 38.08402088671292 40.0 37.5 41.0 33.0 41.0 28-29 37.9217636666842 40.0 37.0 41.0 33.0 41.0 30-31 37.97959041605027 40.0 37.0 41.0 33.0 41.0 32-33 37.895850856423294 40.0 37.0 41.0 33.0 41.0 34-35 37.763309776701874 40.0 37.0 41.0 33.0 41.0 36-37 37.58444762874195 40.0 37.0 41.0 33.0 41.0 38-39 37.45157461399846 40.0 37.0 41.0 32.0 41.0 40-41 37.25191879216315 39.5 36.0 41.0 32.0 41.0 42-43 37.05748884746945 39.0 36.0 41.0 31.0 41.0 44-45 36.76923731409318 39.0 36.0 40.5 31.0 41.0 46-47 36.465970693364085 39.0 35.5 40.0 30.0 41.0 48-49 36.158836653935865 39.0 35.0 40.0 30.0 41.0 50-51 35.906471009166445 39.0 35.0 40.0 29.0 41.0 52-53 35.64791506352954 38.0 35.0 40.0 28.0 41.0 54-55 35.40730438264727 38.0 34.5 40.0 28.0 41.0 56-57 35.0634417993265 38.0 34.0 40.0 27.0 41.0 58-59 34.76114755815603 38.0 34.0 40.0 26.5 41.0 60-61 34.37843200260244 37.0 33.5 40.0 26.0 41.0 62-63 33.95921420239904 37.0 33.0 39.5 25.5 41.0 64-65 33.49975381117538 36.0 32.5 39.0 24.5 40.0 66-67 33.01578083625526 35.5 32.0 39.0 23.0 40.0 68-69 32.53078443184967 35.0 31.0 38.0 22.0 40.0 70-71 32.05587580237888 35.0 31.0 37.5 21.5 39.5 72-73 31.54092038377169 34.5 30.5 37.0 20.0 39.0 74-75 31.025841428761485 34.0 30.0 36.5 18.5 39.0 76-77 29.767090786217494 32.5 28.5 35.0 18.0 37.5 78-79 30.432413653800168 34.0 30.0 35.5 18.0 37.5 80-81 30.231822800621085 34.0 30.0 35.0 17.5 37.0 82-83 29.786522023734015 34.0 29.0 35.0 11.5 36.5 84-85 29.289275846843147 34.0 29.0 35.0 7.0 36.0 86-87 28.836275370819152 33.5 29.0 35.0 4.5 36.0 88-89 28.28885374571656 33.0 27.5 35.0 2.0 35.0 90-91 27.62597000385858 33.0 26.0 35.0 2.0 35.0 92-93 27.007124227234492 32.0 25.5 34.0 2.0 35.0 94-95 26.380523281639576 32.0 25.0 34.0 2.0 35.0 96-97 25.53769473558127 32.0 23.0 34.0 2.0 35.0 98-99 24.3360819998842 31.0 10.0 34.0 2.0 35.0 100-101 21.25961186143945 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24320853524735497 1101 2 0.3077707911878207 1101 3 0.24428702099126554 1101 4 0.21115155212496717 1101 5 0.20243493448562333 1101 6 0.16504746488570987 1101 7 0.21466794018178348 1101 8 0.2367688659841889 1101 9 0.2668464498638059 1101 10-11 0.2740711245677758 1101 12-13 0.30582989143142214 1101 14-15 0.3771746716116908 1101 16-17 0.4288784943717303 1101 18-19 0.26557387134364774 1101 20-21 0.3457522793143539 1101 22-23 0.2850895313794126 1101 24-25 0.3157649915412577 1101 26-27 0.3099874644609315 1101 28-29 0.14521607985301443 1101 30-31 0.37788525533108697 1101 32-33 0.24922348695376684 1101 34-35 0.2000071691182015 1101 36-37 0.3502279826944914 1101 38-39 0.37668491793912295 1101 40-41 0.385824409453015 1101 42-43 0.4045007816456163 1101 44-45 0.3446424798902541 1101 46-47 0.4336899007340662 1101 48-49 0.2553564889600253 1101 50-51 0.23913037497480616 1101 52-53 0.297679570796447 1101 54-55 0.19340440194304875 1101 56-57 0.2989530362628372 1101 58-59 0.41927362579677663 1101 60-61 0.43622320422062444 1101 62-63 0.4512012185543526 1101 64-65 0.40546001963126344 1101 66-67 0.3640964749943336 1101 68-69 0.4509116624891689 1101 70-71 0.33825262555837554 1101 72-73 0.449358073291382 1101 74-75 0.5562474232640504 1101 76-77 0.6969121580598241 1101 78-79 0.6449758798654734 1101 80-81 0.6371199155035292 1101 82-83 0.5923403797861475 1101 84-85 0.7669864031873992 1101 86-87 0.9637264675734301 1101 88-89 1.2038245890990176 1101 90-91 1.352473762859951 1101 92-93 1.4735052917793787 1101 94-95 1.6753290599552741 1101 96-97 1.7903748547619571 1101 98-99 2.2079888150013183 1101 100-101 2.8582348401888034 1102 1 0.3031851601360174 1102 2 0.31484342176688074 1102 3 0.26185584795713623 1102 4 0.18422550484194034 1102 5 0.22320344284675286 1102 6 0.19977520224489353 1102 7 0.19956596782827773 1102 8 0.2373533334648883 1102 9 0.3140943130503331 1102 10-11 0.25056433762303243 1102 12-13 0.30246534643433876 1102 14-15 0.4243182168608257 1102 16-17 0.3544812376733617 1102 18-19 0.47517793857713286 1102 20-21 0.4018115548652972 1102 22-23 0.49967081101875266 1102 24-25 0.2937924765819844 1102 26-27 0.3824758733298381 1102 28-29 0.39762844336738823 1102 30-31 0.4754105073580135 1102 32-33 0.49310142330637063 1102 34-35 0.4832029217725662 1102 36-37 0.39211239698470024 1102 38-39 0.3669103361751169 1102 40-41 0.4422175382825273 1102 42-43 0.5640193225220074 1102 44-45 0.5657878962246627 1102 46-47 0.6181867124442277 1102 48-49 0.5581740599397307 1102 50-51 0.6083732671380417 1102 52-53 0.5752194614195787 1102 54-55 0.43633432773648195 1102 56-57 0.6027741404184255 1102 58-59 0.5438050247783508 1102 60-61 0.5736232029648676 1102 62-63 0.6649966603179465 1102 64-65 0.7220218152273077 1102 66-67 0.6832950118522945 1102 68-69 0.7300352482994228 1102 70-71 0.6070565392301219 1102 72-73 0.8506222667648267 1102 74-75 0.6151469632066977 1102 76-77 0.6265750487699648 1102 78-79 0.645494330147617 1102 80-81 0.7284007119436247 1102 82-83 0.740200426913102 1102 84-85 0.9757443559725409 1102 86-87 1.1097285252290483 1102 88-89 1.1039341094786899 1102 90-91 1.3429119430456424 1102 92-93 1.4810366342516694 1102 94-95 1.5323265690617873 1102 96-97 1.8132370770479618 1102 98-99 2.280218357518276 1102 100-101 3.07209091731411 1103 1 0.25668432163198673 1103 2 0.2795564934075898 1103 3 0.22084755964940683 1103 4 0.15776002953618473 1103 5 0.14315909468016486 1103 6 0.1272663219151866 1103 7 0.10139021403500692 1103 8 0.15031317363290242 1103 9 0.17697290715392455 1103 10-11 0.23105849227795972 1103 12-13 0.30255231274257 1103 14-15 0.3758803084795943 1103 16-17 0.2792831916714391 1103 18-19 0.37402393752238083 1103 20-21 0.38409721546974396 1103 22-23 0.1868318865734082 1103 24-25 0.267099121645046 1103 26-27 0.2563937938770451 1103 28-29 0.31975192808410924 1103 30-31 0.2487405914688523 1103 32-33 0.30021387213808737 1103 34-35 0.3156215431659035 1103 36-37 0.35117619305010805 1103 38-39 0.43518791030808046 1103 40-41 0.33825187462728223 1103 42-43 0.3553392478519015 1103 44-45 0.3131450970814882 1103 46-47 0.37219976576155034 1103 48-49 0.3523792204367453 1103 50-51 0.16501065669780957 1103 52-53 0.3218725607220847 1103 54-55 0.2913773969867819 1103 56-57 0.3810526539735406 1103 58-59 0.2886418769142338 1103 60-61 0.3718873182477793 1103 62-63 0.37703698292109067 1103 64-65 0.4734227168865459 1103 66-67 0.5691128997522554 1103 68-69 0.5902269090210623 1103 70-71 0.5562142682096223 1103 72-73 0.44390577041880874 1103 74-75 0.5511339936002599 1103 76-77 0.4331181705720937 1103 78-79 0.39448119590976916 1103 80-81 0.6108417027456561 1103 82-83 0.7028838816427161 1103 84-85 0.7655645542929292 1103 86-87 0.8706353559442483 1103 88-89 1.2005383309256032 1103 90-91 1.5773720868981478 1103 92-93 1.4992324972597366 1103 94-95 1.6343520934407252 1103 96-97 1.7152643686566904 1103 98-99 1.934609970059654 1103 100-101 2.570913663235764 1104 1 0.12112551698059093 1104 2 0.21255679760402302 1104 3 0.2313135614432298 1104 4 0.12282504746197986 1104 5 0.06257285020985393 1104 6 0.06990722499812563 1104 7 0.12471276952218346 1104 8 0.1017511088599008 1104 9 0.11248383575991738 1104 10-11 0.1743991810085248 1104 12-13 0.17293117019144688 1104 14-15 0.20074642667492526 1104 16-17 0.19578270325547464 1104 18-19 0.1771360907914925 1104 20-21 0.2853171177559588 1104 22-23 0.16931678716847642 1104 24-25 0.12595837926424736 1104 26-27 0.15286203689692002 1104 28-29 0.08429183909660765 1104 30-31 0.16622486971356665 1104 32-33 0.2701277736476584 1104 34-35 0.2402915301816293 1104 36-37 0.25640074298372895 1104 38-39 0.3305389818691964 1104 40-41 0.3644909684392914 1104 42-43 0.3915370944623291 1104 44-45 0.37677530587190944 1104 46-47 0.2823014966220754 1104 48-49 0.4426033870178543 1104 50-51 0.3015744950259105 1104 52-53 0.37170265238444244 1104 54-55 0.3641106507433918 1104 56-57 0.42291723474362186 1104 58-59 0.6095746644147653 1104 60-61 0.6510615444158248 1104 62-63 0.7133118059527632 1104 64-65 0.6787409730895462 1104 66-67 0.7550865759576126 1104 68-69 0.7610416220334244 1104 70-71 0.6461410258904863 1104 72-73 0.8131439390224067 1104 74-75 0.7637303073359618 1104 76-77 0.8003153933025828 1104 78-79 0.8748398762058152 1104 80-81 0.9032417820546961 1104 82-83 0.761039606128314 1104 84-85 0.9497382900374269 1104 86-87 1.0716951488038724 1104 88-89 1.2104247151809595 1104 90-91 1.2780695590467737 1104 92-93 1.3476116044135544 1104 94-95 1.625527337548327 1104 96-97 1.8344930251466387 1104 98-99 2.1144381516513526 1104 100-101 2.85915298151453 1105 1 0.1725107143103628 1105 2 0.18305926620789847 1105 3 0.18764230822807804 1105 4 0.1350444034799807 1105 5 0.07152436037617349 1105 6 0.07016872727461276 1105 7 -0.018629460857219726 1105 8 0.13637609930070482 1105 9 0.2063844163994233 1105 10-11 0.1557785215825973 1105 12-13 0.12373992473556683 1105 14-15 0.17358824018015184 1105 16-17 0.24001780909674864 1105 18-19 0.2822440584040109 1105 20-21 0.37047518487736397 1105 22-23 0.32652796424580544 1105 24-25 0.2700866086401703 1105 26-27 0.21442698440061747 1105 28-29 0.20802233134386938 1105 30-31 0.2712878857935337 1105 32-33 0.29735515938962465 1105 34-35 0.3635820196683781 1105 36-37 0.3831126782684464 1105 38-39 0.32983169468485585 1105 40-41 0.24196801238174004 1105 42-43 0.057555694324676665 1105 44-45 0.15809523954508364 1105 46-47 0.25522724452418544 1105 48-49 0.3314339570443181 1105 50-51 0.34307324626860947 1105 52-53 0.24326381351423976 1105 54-55 0.39802937986471676 1105 56-57 0.4777085831590355 1105 58-59 0.26219462998300713 1105 60-61 0.4276836698958846 1105 62-63 0.5723971448518768 1105 64-65 0.6585687397071567 1105 66-67 0.6844600709672477 1105 68-69 0.8011564815925283 1105 70-71 0.8103838536962726 1105 72-73 0.5903599469905849 1105 74-75 0.670999026105374 1105 76-77 0.6928282585804126 1105 78-79 0.6926633453823037 1105 80-81 0.691425963964786 1105 82-83 0.6743895584722317 1105 84-85 0.8142643498024462 1105 86-87 1.14918991223011 1105 88-89 1.3528861006953754 1105 90-91 1.3979174326154613 1105 92-93 1.3890142041416063 1105 94-95 1.6008078183245544 1105 96-97 1.921015026217269 1105 98-99 1.9982400254401362 1105 100-101 2.530188713319884 1106 1 -0.008118549401423536 1106 2 0.11267688062477532 1106 3 0.053803861674360576 1106 4 0.07482779333943057 1106 5 0.04872558040892727 1106 6 0.07587424303336832 1106 7 0.033827290844385516 1106 8 -0.018115204473474478 1106 9 0.04914338232077853 1106 10-11 -0.11793470087159363 1106 12-13 -0.19491679747415702 1106 14-15 0.027253771150512307 1106 16-17 0.03668134548430402 1106 18-19 0.08940554200108863 1106 20-21 0.21111015294075486 1106 22-23 0.16224904085486713 1106 24-25 0.2635172910668899 1106 26-27 0.3054899728599807 1106 28-29 0.24687692082458312 1106 30-31 0.2244976507121592 1106 32-33 0.1903490902699616 1106 34-35 -0.1671607489988176 1106 36-37 0.3208025322836079 1106 38-39 0.33325411162251584 1106 40-41 0.33224655548257687 1106 42-43 0.18603922931524863 1106 44-45 0.2937670336606715 1106 46-47 0.4132859023329374 1106 48-49 0.24336420097326084 1106 50-51 0.32072402353501417 1106 52-53 0.25589798219230175 1106 54-55 0.11636751209701401 1106 56-57 0.18738250379481514 1106 58-59 0.2632536215157373 1106 60-61 0.3458980138178305 1106 62-63 0.3077336627914775 1106 64-65 0.18750377218702852 1106 66-67 0.11753463887231419 1106 68-69 0.34769875200208133 1106 70-71 0.43745119608930594 1106 72-73 0.44921546779400146 1106 74-75 0.3978995065126725 1106 76-77 0.2302447305747677 1106 78-79 0.30660621649500186 1106 80-81 0.4068154896886931 1106 82-83 0.3538736833005167 1106 84-85 0.5941891066884146 1106 86-87 0.899544979013708 1106 88-89 0.9248317502541639 1106 90-91 0.8497888091880377 1106 92-93 0.9203471985769518 1106 94-95 0.5061173279055353 1106 96-97 0.9434587394009171 1106 98-99 1.3805805946335319 1106 100-101 1.775188092438075 1107 1 0.0880092531371588 1107 2 0.08835129504969785 1107 3 0.07037314927264049 1107 4 0.0325250490154545 1107 5 0.041122603226526167 1107 6 0.06820465604569392 1107 7 0.028809089419482348 1107 8 0.01874997255094968 1107 9 -0.008965198756364146 1107 10-11 -0.05764238236162811 1107 12-13 0.02358819471397311 1107 14-15 0.10654190546111408 1107 16-17 0.031854696517193304 1107 18-19 0.01715636930275366 1107 20-21 0.141412056956419 1107 22-23 0.14804518962569801 1107 24-25 -0.0542603535788686 1107 26-27 0.0432639204644687 1107 28-29 0.09848111353738886 1107 30-31 0.04395571491297545 1107 32-33 0.10828009512420067 1107 34-35 0.050908914609841815 1107 36-37 0.12904945680248403 1107 38-39 0.11452623277826035 1107 40-41 0.07584216065433935 1107 42-43 0.18084859126425812 1107 44-45 0.16299681147559397 1107 46-47 0.15030920867025088 1107 48-49 0.31759471977298404 1107 50-51 0.27281364013055764 1107 52-53 0.3098822733142015 1107 54-55 0.2272511601386853 1107 56-57 0.15393748407989705 1107 58-59 0.3018719370838241 1107 60-61 0.41772860892323394 1107 62-63 0.31696779192148483 1107 64-65 0.4731297703487627 1107 66-67 0.5268587897746642 1107 68-69 0.6106752485943332 1107 70-71 0.4696040249959097 1107 72-73 0.49756216513008056 1107 74-75 0.4054335090446344 1107 76-77 0.4653503445595142 1107 78-79 0.5486786643146928 1107 80-81 0.6452180919643062 1107 82-83 0.62942583523078 1107 84-85 0.8577453844851988 1107 86-87 0.9717050758777681 1107 88-89 1.2166396507288653 1107 90-91 1.2517307017893806 1107 92-93 1.5325652104989267 1107 94-95 1.4676217872152222 1107 96-97 1.6389277749067013 1107 98-99 2.009790216084884 1107 100-101 2.4858238652931206 1108 1 0.07542607262186607 1108 2 0.11765592147622073 1108 3 0.0642263541940622 1108 4 0.0724102715071524 1108 5 -0.01610179401319556 1108 6 0.001441420903304902 1108 7 0.06355475429018043 1108 8 0.10279887534869658 1108 9 0.016832124047901686 1108 10-11 -0.0029934214923841296 1108 12-13 0.0020683708155999625 1108 14-15 0.05515879506121024 1108 16-17 0.11826251415274669 1108 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1.1354258318945334 2316 100-101 1.5891885210832193 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 20.0 5 47.0 6 92.0 7 234.0 8 569.0 9 1129.0 10 1870.0 11 2911.0 12 4564.0 13 6443.0 14 8510.0 15 11542.0 16 14453.0 17 19052.0 18 17399.0 19 13431.0 20 12728.0 21 13301.0 22 14622.0 23 16524.0 24 19453.0 25 23230.0 26 28645.0 27 35679.0 28 44157.0 29 54738.0 30 68177.0 31 85001.0 32 107850.0 33 139239.0 34 185836.0 35 256161.0 36 359797.0 37 440225.0 38 242130.0 39 12721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.64126373504897 19.908027680130882 15.785589529305122 22.665119055515024 2 15.728253264435759 20.644918052640257 39.5707559241172 24.05607275880678 3 19.4555912045617 23.938747159657208 30.051301861532266 26.554359774248827 4 13.405799183289568 17.36409117207538 36.923417595915296 32.30669204871975 5 13.845077419742651 37.326296192836864 34.49969767581654 14.328928711603945 6 32.36198508634951 36.8747544189126 16.853326709365916 13.909933785371964 7 28.789639384200864 32.51199673280468 20.37148435065598 18.326879532338474 8 26.62180755636155 35.43788682474317 19.3014248454469 18.63888077344838 9 25.916080992145385 16.908663946066525 19.96467610257897 37.210578959209116 10-11 24.932474869513293 26.885698108854356 27.252572496420978 20.92925452521137 12-13 25.758985845642474 23.83377663542004 27.93852926843553 22.46870825050196 14-15 22.44892907861291 25.49227820132765 25.948036920367862 26.110755799691574 16-17 22.21425411195006 28.73843476062446 26.849631667043095 22.197679460382382 18-19 22.177679380824056 27.172616377258517 29.123209771884174 21.52649447003325 20-21 22.606336957585878 26.26367989774096 29.54782773577513 21.58215540889803 22-23 22.32528515727988 26.166844826660306 29.445924261413197 22.061945754646615 24-25 22.300606853510953 26.4106360691097 29.397367502176806 21.891389575202542 26-27 22.026363763538075 26.750510450675076 29.136031707058883 22.08709407872797 28-29 22.04433557414228 26.938283533568153 29.184433354092747 21.832947538196812 30-31 22.20075372758801 26.62245576054862 29.031970770238168 22.144819741625206 32-33 22.047024317024302 26.588129268252796 29.053599818606852 22.311246596116046 34-35 22.488111554918895 26.681918292713654 28.939982523687384 21.889987628680068 36-37 22.59876392878469 26.382999976574496 28.95759585341997 22.06064024122085 38-39 22.15226981063567 26.744200693881364 28.940386711016025 22.16314278446694 40-41 22.54930516850675 26.600812997709163 28.912777995580978 21.93710383820311 42-43 22.34668517292708 26.57294188761646 28.766508383623286 22.313864555833174 44-45 22.37120501252823 26.796592782026536 28.71573853774446 22.116463667700778 46-47 22.727184329131035 27.059466755889854 28.132200304885192 22.08114861009392 48-49 22.622233635404516 27.189677218605514 27.932597300939538 22.255491845050436 50-51 22.64530555038672 27.295909685386594 27.992022951693507 22.06676181253318 52-53 22.954792517360847 27.110522674017858 27.891697224806027 22.04298758381527 54-55 22.61852091345336 27.257633290032977 27.948287971090274 22.175557825423393 56-57 22.61312862681001 27.35796518085597 27.873702039035734 22.15520415329829 58-59 22.54837698801896 27.08695857795678 27.952531081891596 22.41213335213267 60-61 22.387426071529124 27.25522444067181 28.172797151458283 22.184552336340786 62-63 22.42660854783511 27.285876496304297 28.22979185331566 22.057723102544937 64-65 22.315978273504737 27.246716119533737 28.16517281173715 22.27213279522438 66-67 22.315182690229484 27.33199822673328 27.928928778059227 22.42389030497801 68-69 22.563603567925796 27.068063475169623 28.115448857034124 22.25288409987045 70-71 22.517084045859185 27.17268267586479 27.862563873182257 22.447669405093766 72-73 22.463856674440567 27.222826400434393 27.779117258431047 22.534199666693997 74-75 22.38492882402626 27.449965546824274 27.617678921153722 22.547426707995744 76-77 22.457746793246912 27.18147829096337 27.89493969257778 22.46583522321194 78-79 22.508818819610774 27.279754924991966 27.816000152044808 22.395426103352456 80-81 22.59701806548623 27.349169565757386 27.59568988340727 22.458122485349115 82-83 22.590090259321517 27.39161124447012 27.633933721432356 22.384364774776007 84-85 22.453067112901472 27.450004110522674 27.641961099427796 22.45496767714806 86-87 22.476487199286098 27.50026408944831 27.50061768201509 22.522631029250505 88-89 22.4348516745481 27.564220144964114 27.350385040205683 22.650543140282107 90-91 22.31866408666507 27.461148027039183 27.532492217449487 22.68769566884626 92-93 22.426051665931478 27.177830483483007 27.7433216439819 22.652796206603615 94-95 22.28274057222769 27.111864754379134 27.74304958561161 22.862345087781566 96-97 22.46826617210853 26.8619190087386 27.796154769234445 22.87366004991843 98-99 22.2156046186404 27.07254504341246 27.543553899165694 23.168296438781447 100-101 22.256328421480717 26.718547971788713 28.05133464325893 22.97378896347164 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 215.0 1 157.5 2 139.0 3 191.5 4 171.0 5 182.5 6 362.5 7 848.0 8 1414.0 9 1672.5 10 1584.5 11 1454.0 12 1474.5 13 1598.0 14 1819.5 15 2047.0 16 2234.5 17 2400.5 18 2599.5 19 2928.5 20 3382.0 21 3889.0 22 4617.5 23 5708.0 24 7222.0 25 9016.5 26 11595.0 27 15071.0 28 17994.0 29 21209.0 30 25351.0 31 30848.5 32 37973.0 33 42518.5 34 45241.5 35 49035.5 36 54395.0 37 59960.0 38 63999.0 39 67106.5 40 70542.0 41 75340.5 42 77745.0 43 78732.5 44 81444.0 45 84617.0 46 86427.5 47 87541.0 48 88019.0 49 86288.5 50 83521.5 51 81064.0 52 79446.0 53 78982.5 54 77699.0 55 71557.5 56 63839.5 57 57482.5 58 52096.5 59 45456.0 60 36267.5 61 29448.5 62 24422.0 63 17921.0 64 12222.0 65 8716.5 66 6327.0 67 4885.5 68 3673.5 69 2725.5 70 2106.5 71 1394.0 72 916.0 73 687.0 74 473.0 75 334.0 76 244.5 77 143.5 78 90.0 79 57.5 80 28.5 81 17.5 82 11.0 83 5.5 84 3.5 85 2.5 86 2.0 87 1.0 88 0.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041591325667151825 2 8.83981416942653E-5 3 0.0 4 0.0 5 8.397823460955204E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005016594541149556 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.629860627069898E-5 22-23 5.082893147420255E-4 24-25 4.419907084713265E-5 26-27 0.0013038725899904133 28-29 0.0028287405342164898 30-31 1.1049767711783162E-4 32-33 8.83981416942653E-5 34-35 0.0 36-37 0.0 38-39 2.2099535423566326E-5 40-41 0.0 42-43 0.008552520208920167 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.2099535423566326E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.4309488965922957E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.419907084713265E-4 84-85 2.2099535423566324E-4 86-87 0.0 88-89 0.0 90-91 0.009657496980098483 92-93 0.0014585693379553775 94-95 0.0 96-97 0.0 98-99 3.0939349592992856E-4 100-101 0.012198943553808612 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2262491.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.20778324649469 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98612140012773 40.84609753507094 2 14.400697018495686 15.900611191911047 3 4.935566396835156 8.174450395054745 4 2.286172560298747 5.048580766922282 5 1.2495837334362963 3.4493373951948327 6 0.735734796143153 2.437097229142509 7 0.48577024443287686 1.8772808853572904 8 0.35037340967006747 1.5474671405120297 9 0.2472144532808794 1.2283345756918331 >10 1.2255553203673628 11.738438848997209 >50 0.06603146251545237 2.475278051849736 >100 0.027647078573849283 2.7860232490862633 >500 0.002006889671959188 0.7656441947351905 >1k 0.0013646849769318875 1.1817050711052963 >5k 1.6055117375669267E-4 0.543653469368762 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6804 0.3007304780438905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5463 0.24145952403788568 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3874 0.17122720046179188 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2270 0.10033189082299113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2099535423566326E-5 0.0 12-13 0.0 0.0 0.0 4.419907084713265E-5 6.629860627069898E-5 14-15 4.419907084713265E-5 0.0 0.0 4.419907084713265E-5 8.83981416942653E-5 16-17 4.419907084713265E-5 0.0 0.0 6.629860627069898E-5 1.3259721254139795E-4 18-19 4.419907084713265E-5 0.0 0.0 8.83981416942653E-5 1.3259721254139795E-4 20-21 4.419907084713265E-5 0.0 0.0 1.1049767711783162E-4 1.767962833885306E-4 22-23 4.419907084713265E-5 0.0 0.0 2.2099535423566324E-4 1.767962833885306E-4 24-25 4.419907084713265E-5 0.0 0.0 3.0939349592992856E-4 1.767962833885306E-4 26-27 4.419907084713265E-5 0.0 0.0 5.082893147420255E-4 2.2099535423566324E-4 28-29 6.629860627069898E-5 0.0 0.0 0.0010386781649076174 2.651944250827959E-4 30-31 8.83981416942653E-5 0.0 0.0 0.0025414465737101273 2.651944250827959E-4 32-33 8.83981416942653E-5 0.0 0.0 0.00517129128911452 2.651944250827959E-4 34-35 8.83981416942653E-5 0.0 0.0 0.008972411381967929 3.0939349592992856E-4 36-37 8.83981416942653E-5 0.0 0.0 0.012729332403974204 3.0939349592992856E-4 38-39 8.83981416942653E-5 0.0 0.0 0.016640950173945443 3.0939349592992856E-4 40-41 8.83981416942653E-5 0.0 0.0 0.02088406097527018 3.0939349592992856E-4 42-43 8.83981416942653E-5 0.0 0.0 0.026431044366585325 3.0939349592992856E-4 44-45 8.83981416942653E-5 0.0 0.0 0.032751511497725296 3.0939349592992856E-4 46-47 1.3259721254139795E-4 0.0 0.0 0.04234270987155308 3.3149303135349487E-4 48-49 1.3259721254139795E-4 0.0 0.0 0.05504994274010372 3.535925667770612E-4 50-51 1.3259721254139795E-4 0.0 0.0 0.06844226120678491 3.535925667770612E-4 52-53 1.3259721254139795E-4 0.0 0.0 0.09067439384289264 3.535925667770612E-4 54-55 1.3259721254139795E-4 0.0 0.0 0.12853089802346174 3.535925667770612E-4 56-57 1.3259721254139795E-4 0.0 0.0 0.18576869477049854 3.756921022006276E-4 58-59 1.3259721254139795E-4 0.0 0.0 0.23920537142468193 3.977916376241939E-4 60-61 1.3259721254139795E-4 0.0 0.0 0.2919569624807347 3.977916376241939E-4 62-63 1.3259721254139795E-4 0.0 0.0 0.35653180498839554 4.4199070847132654E-4 64-65 1.3259721254139795E-4 0.0 0.0 0.4310293389012376 4.4199070847132654E-4 66-67 1.3259721254139795E-4 0.0 0.0 0.5317148222910058 4.4199070847132654E-4 68-69 1.3259721254139795E-4 0.0 0.0 0.6555827183400951 4.4199070847132654E-4 70-71 1.3259721254139795E-4 0.0 0.0 0.7830749381986493 4.4199070847132654E-4 72-73 1.3259721254139795E-4 0.0 0.0 0.9205340485332317 4.4199070847132654E-4 74-75 1.3259721254139795E-4 0.0 0.0 1.0751202988210782 4.4199070847132654E-4 76-77 1.3259721254139795E-4 0.0 0.0 1.243695555032042 4.4199070847132654E-4 78-79 1.3259721254139795E-4 4.419907084713265E-5 0.0 1.4278288841811966 4.4199070847132654E-4 80-81 1.3259721254139795E-4 4.419907084713265E-5 0.0 1.6226583884753576 4.4199070847132654E-4 82-83 1.9889581881209694E-4 4.419907084713265E-5 0.0 1.8436095436401736 4.4199070847132654E-4 84-85 2.2099535423566327E-4 4.419907084713265E-5 0.0 2.0970912149484793 4.4199070847132654E-4 86-87 2.430948896592296E-4 4.419907084713265E-5 0.0 2.3709928569881606 4.4199070847132654E-4 88-89 2.651944250827959E-4 4.419907084713265E-5 0.0 2.684717861861108 4.4199070847132654E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4530 0.0 45.94248 1 GTATCAA 7700 0.0 39.24686 1 ATCAACG 9445 0.0 31.682096 3 TCAACGC 9585 0.0 31.219341 4 TATCAAC 9730 0.0 30.7541 2 CAACGCA 9790 0.0 30.56562 5 AACGCAG 10175 0.0 29.455761 6 ACGCAGA 11505 0.0 25.889486 7 CGCAGAG 11620 0.0 25.592382 8 GCAGAGT 13015 0.0 22.447783 9 GAGTACT 7715 0.0 21.705296 12-13 TATACCG 285 8.367351E-11 21.665752 5 GTACATG 6980 0.0 20.96688 1 AGAGTAC 11175 0.0 20.872595 10-11 GTACTTT 8040 0.0 20.706528 14-15 TACATGG 6930 0.0 20.698984 2 ACATGGG 6795 0.0 19.851995 3 TAAGGTG 1295 0.0 19.806116 5 AGTACTT 8295 0.0 19.786737 12-13 CAGAGTA 12285 0.0 19.566713 10-11 >>END_MODULE