FastQCFastQC Report
Thu 26 May 2016
SRR937218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225942
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59820.26874015585311745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44690.20076893288324674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.14092909878154958No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30780.13827853555932723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.046.765621
GTATCAA64800.042.047941
ATCAACG80450.033.2645033
TATCAAC81550.033.0497062
TCAACGC81650.032.776364
CAACGCA82250.032.5943225
AACGCAG85750.031.1888376
ACGCAGA98350.027.1442037
CGCAGAG99150.026.7808538
GTACATG73700.023.93711
TACATGG71500.023.069172
GCAGAGT112050.022.976979
ACATGGG71950.021.8012753
GAGTACT62900.021.59714112-13
GTATAGG5300.021.5327991
AGAGTAC96500.020.42709210-11
GTACTTT66800.020.12268614-15
TAAGGTG10700.019.9906755
CAGAGTA103600.019.92121510-11
CATGGGG43500.019.3416084
Produced by FastQC (version 0.11.5)