##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937218_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2225942 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.651001688273997 31.0 31.0 34.0 30.0 34.0 2 31.776985204466243 33.0 31.0 34.0 30.0 34.0 3 31.818881624049503 33.0 31.0 34.0 30.0 34.0 4 35.45401272809444 37.0 35.0 37.0 33.0 37.0 5 35.4587671197183 37.0 35.0 37.0 33.0 37.0 6 35.49515216479136 37.0 35.0 37.0 33.0 37.0 7 35.43773018344593 37.0 35.0 37.0 33.0 37.0 8 35.41669279792555 37.0 35.0 37.0 33.0 37.0 9 37.0400522565278 39.0 37.0 39.0 33.0 39.0 10-11 36.91288497184563 39.0 37.0 39.0 33.0 39.0 12-13 36.78204822946869 39.0 37.0 39.0 33.0 39.0 14-15 37.94960156194546 40.0 38.0 41.0 33.0 41.0 16-17 37.89751170515674 40.0 38.0 41.0 33.0 41.0 18-19 37.85002282179859 40.0 38.0 41.0 33.0 41.0 20-21 37.76629175423259 40.0 37.5 41.0 32.5 41.0 22-23 37.61558162791304 40.0 37.0 41.0 32.0 41.0 24-25 37.45217238364701 40.0 37.0 41.0 32.0 41.0 26-27 37.28697198758997 39.0 37.0 41.0 32.0 41.0 28-29 37.35090761574201 40.0 37.0 41.0 32.0 41.0 30-31 37.204793745748994 39.0 37.0 41.0 31.5 41.0 32-33 37.01262386890584 39.0 36.0 41.0 31.0 41.0 34-35 36.89330090361744 39.0 36.0 41.0 31.0 41.0 36-37 36.89948075915724 39.0 36.0 41.0 31.0 41.0 38-39 36.804565437913475 39.0 36.0 41.0 30.0 41.0 40-41 36.62543071652361 39.0 36.0 41.0 30.0 41.0 42-43 36.392943526830436 39.0 35.5 41.0 30.0 41.0 44-45 36.190334923371765 39.0 35.0 41.0 30.0 41.0 46-47 35.89710221560131 39.0 35.0 40.5 28.5 41.0 48-49 35.59804792757404 39.0 35.0 40.0 27.5 41.0 50-51 34.74260762409802 38.0 34.0 39.5 26.0 40.5 52-53 34.846224654550745 38.0 34.5 39.5 26.5 40.5 54-55 35.166396294243064 38.0 35.0 40.0 26.5 41.0 56-57 34.94810017511687 38.0 34.0 40.0 26.0 41.0 58-59 34.68925852515474 38.0 34.0 40.0 26.0 41.0 60-61 34.38781378849943 38.0 34.0 40.0 25.0 41.0 62-63 34.04034606472226 37.0 33.5 40.0 24.0 41.0 64-65 33.66127262076011 37.0 33.0 39.5 23.0 41.0 66-67 33.27823029530868 36.0 33.0 39.0 22.0 41.0 68-69 32.790205450097076 36.0 32.0 39.0 21.5 40.0 70-71 32.336117023714 35.0 32.0 38.5 20.0 40.0 72-73 31.93439653863398 35.0 31.0 37.5 18.5 40.0 74-75 31.474887935085462 35.0 31.0 37.0 17.5 39.0 76-77 31.023618315302016 35.0 31.0 36.5 14.5 39.0 78-79 30.541079012840406 34.0 30.0 36.0 9.5 38.5 80-81 30.050701905081084 34.0 30.0 35.5 7.0 37.0 82-83 29.555521662289493 34.0 29.0 35.0 4.0 37.0 84-85 29.100023495670598 34.0 29.0 35.0 2.0 36.5 86-87 28.65227553098868 34.0 29.0 35.0 2.0 36.0 88-89 28.21326925858805 34.0 27.5 35.0 2.0 36.0 90-91 27.76344464500872 33.0 27.0 35.0 2.0 35.0 92-93 27.280027062699745 33.0 26.0 35.0 2.0 35.0 94-95 26.69669515198509 33.0 25.0 35.0 2.0 35.0 96-97 26.00112716324145 32.0 24.5 34.5 2.0 35.0 98-99 25.16117872792732 32.0 20.5 34.0 2.0 35.0 100-101 23.14424746916137 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10981218961704897 1101 2 0.11221278392978107 1101 3 0.10090884332849726 1101 4 0.0804190289479152 1101 5 0.18614507281868953 1101 6 0.1758554871580884 1101 7 0.051013491519832144 1101 8 0.14862153892833874 1101 9 0.07082837874328618 1101 10-11 0.0689075045815315 1101 12-13 0.0035738922125858608 1101 14-15 0.10008923915437862 1101 16-17 0.2682275422146958 1101 18-19 0.27725209188501054 1101 20-21 0.15899154987874198 1101 22-23 0.23431983049184169 1101 24-25 0.343877881672789 1101 26-27 0.35016040571738216 1101 28-29 0.2918174171368335 1101 30-31 0.2484390685674427 1101 32-33 0.3106434222636878 1101 34-35 0.21066628905968798 1101 36-37 0.23300346566946928 1101 38-39 0.29894437969018384 1101 40-41 0.23185859486360272 1101 42-43 0.30516644526232284 1101 44-45 0.3377089181865571 1101 46-47 0.38463300514403187 1101 48-49 0.5256237297933453 1101 50-51 0.43012232970706776 1101 52-53 0.4027773594845101 1101 54-55 0.58596369133042 1101 56-57 0.5693110753390513 1101 58-59 0.5198804899644927 1101 60-61 0.506112903918897 1101 62-63 0.5704414665395845 1101 64-65 0.5944423688110518 1101 66-67 0.5273380353652257 1101 68-69 0.6147264307955922 1101 70-71 0.5472663607035102 1101 72-73 0.5321647057124714 1101 74-75 0.5598894674233819 1101 76-77 0.5914250841710995 1101 78-79 0.47962962505039286 1101 80-81 0.4513934698453035 1101 82-83 0.4281078977165116 1101 84-85 0.5933295460877979 1101 86-87 0.5911430533765696 1101 88-89 0.7230527006988936 1101 90-91 0.78508318382789 1101 92-93 0.8035360590824894 1101 94-95 0.9179618069689575 1101 96-97 0.838674380379679 1101 98-99 0.9533821793951525 1101 100-101 1.4248086431001745 1102 1 0.07182331869040581 1102 2 0.050075372226359605 1102 3 0.17718310753008026 1102 4 0.04205279701547937 1102 5 0.0015916141119447502 1102 6 -0.013187336992537269 1102 7 0.11540156766403697 1102 8 0.123232218757515 1102 9 0.15504448559797623 1102 10-11 0.27770333159774907 1102 12-13 0.2517392563172507 1102 14-15 0.37490341705856167 1102 16-17 0.3212560779189815 1102 18-19 0.335620678905606 1102 20-21 0.3353415939127373 1102 22-23 0.3787743242239472 1102 24-25 0.3878940179575352 1102 26-27 0.3614842267505125 1102 28-29 0.3086558702233262 1102 30-31 0.3358111302830551 1102 32-33 0.3804321359907519 1102 34-35 0.4587381674441673 1102 36-37 0.47334337427906803 1102 38-39 0.6156785286906938 1102 40-41 0.6490393902647185 1102 42-43 0.6046605114826846 1102 44-45 0.4286449734412301 1102 46-47 0.5161102453020092 1102 48-49 0.5288634287180614 1102 50-51 0.37782006012330527 1102 52-53 0.5088475906745202 1102 54-55 0.4485753166129953 1102 56-57 0.46508030814600687 1102 58-59 0.4421067797555338 1102 60-61 0.40962359179776087 1102 62-63 0.2921771186605966 1102 64-65 0.3957238580744544 1102 66-67 0.35010081697386397 1102 68-69 0.43868564162571744 1102 70-71 0.41643557344607274 1102 72-73 0.3869636080157868 1102 74-75 0.6022090173383461 1102 76-77 0.5752371857352188 1102 78-79 0.5529727615663731 1102 80-81 0.3880470673108327 1102 82-83 0.44890505233249556 1102 84-85 0.6130996395786461 1102 86-87 0.5783897724465099 1102 88-89 0.5390475092505653 1102 90-91 0.6556506660762835 1102 92-93 0.5645464807748688 1102 94-95 0.6034422611899224 1102 96-97 0.8073172702266689 1102 98-99 1.1473735086373331 1102 100-101 1.5546709046239968 1103 1 -0.005793667934064217 1103 2 -0.0052916373830882435 1103 3 -0.008424563438406807 1103 4 -0.16288627723947968 1103 5 -0.1761899311283628 1103 6 -0.08704740306484382 1103 7 -0.05547895400163583 1103 8 -0.1320951115217497 1103 9 -0.0233991306959922 1103 10-11 0.06424676767565529 1103 12-13 0.0028785680951699533 1103 14-15 0.055256514707728854 1103 16-17 -0.12735374568773494 1103 18-19 -0.06241718167339627 1103 20-21 -0.20315375067874442 1103 22-23 -0.18947524701116691 1103 24-25 -0.30413665817410873 1103 26-27 -0.10375773421028356 1103 28-29 -0.10129849163418925 1103 30-31 -0.12080481043852132 1103 32-33 -0.09238907821057296 1103 34-35 -0.08351012285368853 1103 36-37 -0.026428158497516563 1103 38-39 -0.24123678704685858 1103 40-41 -0.0010803390033515825 1103 42-43 -0.09766181809493446 1103 44-45 -0.08237087962634604 1103 46-47 -0.19343835392776754 1103 48-49 -0.12672033096069413 1103 50-51 -0.14337243509132946 1103 52-53 -0.08739172131078021 1103 54-55 -0.1632025316750969 1103 56-57 -0.2145647484938138 1103 58-59 -0.1058775586607652 1103 60-61 -0.16036099962611416 1103 62-63 -0.2747131417229056 1103 64-65 -0.2588909033276465 1103 66-67 -0.17161186458153566 1103 68-69 -0.16267499993513468 1103 70-71 0.055575828126542604 1103 72-73 0.0032456478651745613 1103 74-75 -0.11133084552601957 1103 76-77 4.783724651780119E-4 1103 78-79 0.15933686988403295 1103 80-81 0.047443049744089194 1103 82-83 -0.0945953143902365 1103 84-85 -0.0336753433640844 1103 86-87 0.13274164100423747 1103 88-89 0.06792657854214923 1103 90-91 0.039776047920707214 1103 92-93 0.04321711260944383 1103 94-95 0.14356426821745316 1103 96-97 0.1743958346091503 1103 98-99 0.2900001351269488 1103 100-101 0.72607904386388 1104 1 -0.002189891942229849 1104 2 0.023273936276165585 1104 3 0.05283284292855939 1104 4 0.053569631976039034 1104 5 0.09647361963934742 1104 6 0.07139117328417655 1104 7 0.06392945946838324 1104 8 0.12416925676230761 1104 9 0.22651401497771673 1104 10-11 0.24388187093730807 1104 12-13 0.09098956792417567 1104 14-15 0.16978649709259486 1104 16-17 0.21740330647643447 1104 18-19 0.2559741540647167 1104 20-21 0.2584410304619311 1104 22-23 0.2651582955868079 1104 24-25 0.18557721110470737 1104 26-27 0.2101918169971313 1104 28-29 0.08002857461665513 1104 30-31 0.1661147244565626 1104 32-33 0.009476118446620774 1104 34-35 0.20782134039743028 1104 36-37 0.3236297217683699 1104 38-39 0.3104856428114857 1104 40-41 0.42944366400398337 1104 42-43 0.35250245872066444 1104 44-45 0.37148267040853256 1104 46-47 0.44734199148103215 1104 48-49 0.40697745126836793 1104 50-51 0.36621445044824696 1104 52-53 0.39538872995173335 1104 54-55 0.5116538872667462 1104 56-57 0.4660505194110698 1104 58-59 0.5180944389615973 1104 60-61 0.590297532590057 1104 62-63 0.3213138156886117 1104 64-65 0.32269143321658333 1104 66-67 0.47991025748679306 1104 68-69 0.5546467383456388 1104 70-71 0.636764266730026 1104 72-73 0.6812715711673754 1104 74-75 0.48480637616693656 1104 76-77 0.5667132781332356 1104 78-79 0.5277858144682597 1104 80-81 0.47440738247215464 1104 82-83 0.49022980985216336 1104 84-85 0.5848685981395292 1104 86-87 0.5291593750291455 1104 88-89 0.4649424538844791 1104 90-91 0.6490873066841765 1104 92-93 0.703895919882406 1104 94-95 0.8254409595018934 1104 96-97 1.0914733629521578 1104 98-99 1.3222505714342283 1104 100-101 1.46774340346251 1105 1 0.017326954847344922 1105 2 -0.0520050400666392 1105 3 0.012516872631930909 1105 4 -0.014640591069777997 1105 5 -0.06798851397172712 1105 6 -0.02142510135028175 1105 7 -0.04144649919577148 1105 8 0.029817090553272863 1105 9 0.0695541469927008 1105 10-11 0.05815463878841598 1105 12-13 0.04557932053133129 1105 14-15 0.1571585061280416 1105 16-17 0.020382435664458853 1105 18-19 0.06254649854808747 1105 20-21 0.1368398077001629 1105 22-23 0.08254173419848598 1105 24-25 0.0720886203917459 1105 26-27 0.21326630537141966 1105 28-29 0.22934854864649878 1105 30-31 0.05602693990069696 1105 32-33 0.12196614292890473 1105 34-35 0.025220672206828 1105 36-37 -0.15049412343864788 1105 38-39 0.10229726445869858 1105 40-41 0.056894936136103524 1105 42-43 0.08019990634074503 1105 44-45 0.04445950015518463 1105 46-47 0.22728139443784556 1105 48-49 0.20673600992441266 1105 50-51 0.156620369480855 1105 52-53 0.27530615295425775 1105 54-55 0.1494884898015485 1105 56-57 0.04658439559291594 1105 58-59 0.3090488423918174 1105 60-61 0.287584191772261 1105 62-63 0.10531680496239204 1105 64-65 0.3689900089155529 1105 66-67 0.2628220265687702 1105 68-69 0.358516562911781 1105 70-71 0.44900811572436083 1105 72-73 0.4143608706493289 1105 74-75 0.3462579323476973 1105 76-77 0.31539346641690713 1105 78-79 0.20577170671914047 1105 80-81 0.15761875523865498 1105 82-83 0.18503408681311484 1105 84-85 0.35885785839571227 1105 86-87 0.3282318209584574 1105 88-89 0.24140868591163667 1105 90-91 0.46794306407958075 1105 92-93 0.5882789932886929 1105 94-95 0.6326145402731527 1105 96-97 0.804957557674264 1105 98-99 0.9411695634673372 1105 100-101 1.3086335577955985 1106 1 0.046962967582146575 1106 2 -0.046810234871834666 1106 3 -0.015358418415328146 1106 4 0.042059618374594265 1106 5 -0.03747707494635222 1106 6 -0.02411626045854831 1106 7 0.005293954271387236 1106 8 -0.014887951098010888 1106 9 0.061962062747085156 1106 10-11 -0.42020780231678145 1106 12-13 -0.3286787602564374 1106 14-15 -0.2947836060800455 1106 16-17 -0.34170886453548377 1106 18-19 -0.05427521056182627 1106 20-21 -0.16862511859223162 1106 22-23 -0.14137403480594202 1106 24-25 -0.07723159921415146 1106 26-27 -0.0294225143931115 1106 28-29 0.06748515170007607 1106 30-31 0.0071055052981492395 1106 32-33 0.10422276893729077 1106 34-35 -0.03651250736339762 1106 36-37 0.0013076281449784233 1106 38-39 -0.08940756884479129 1106 40-41 -0.24335837092834822 1106 42-43 -0.12523431606505397 1106 44-45 0.1001889733276684 1106 46-47 0.1146714178678323 1106 48-49 0.14383937236551958 1106 50-51 -0.06364392153350451 1106 52-53 -0.31055388484075763 1106 54-55 -0.23573974242218299 1106 56-57 -0.292993553899187 1106 58-59 -0.3170067864712962 1106 60-61 -0.4183017250128884 1106 62-63 -0.09315869223953399 1106 64-65 0.08284157389996238 1106 66-67 0.09824480812158498 1106 68-69 -0.03174957399100009 1106 70-71 -0.14118592187328005 1106 72-73 0.007462548031892169 1106 74-75 -0.07141992174304335 1106 76-77 0.05431109958587754 1106 78-79 0.03274918322518516 1106 80-81 0.09435778316822407 1106 82-83 0.0112109777521745 1106 84-85 0.06730416862924571 1106 86-87 0.18609073659953168 1106 88-89 0.11838369514108038 1106 90-91 0.2183037433665227 1106 92-93 0.4369857748268835 1106 94-95 0.5989904947436742 1106 96-97 0.5977502771979175 1106 98-99 0.6838063876619991 1106 100-101 0.5597494064839239 1107 1 0.14135172002941587 1107 2 0.13992794162743394 1107 3 0.11390250995458473 1107 4 0.091843345409508 1107 5 0.11105275192960562 1107 6 0.1817876027932357 1107 7 0.07201780565853966 1107 8 0.13900813221406594 1107 9 0.16834620972364434 1107 10-11 0.2030330693866702 1107 12-13 0.27928450768051505 1107 14-15 0.277366598532069 1107 16-17 0.29321653997157426 1107 18-19 0.18320871331129496 1107 20-21 0.05424066235280378 1107 22-23 0.2985199306839732 1107 24-25 0.32674901584487515 1107 26-27 0.3868845464229551 1107 28-29 0.3908475755271539 1107 30-31 0.44417850676386905 1107 32-33 0.3232308454928656 1107 34-35 0.3303796150325411 1107 36-37 0.25825055261132945 1107 38-39 0.3441960459440452 1107 40-41 0.35533970808770476 1107 42-43 0.35034770765515333 1107 44-45 0.26334118858775213 1107 46-47 0.28418313759075176 1107 48-49 0.1945480427566153 1107 50-51 0.05496289338574201 1107 52-53 0.09266575580874559 1107 54-55 0.2547563796839256 1107 56-57 0.11176294183723456 1107 58-59 0.1973280430236457 1107 60-61 0.2600815826841725 1107 62-63 0.28993446778358845 1107 64-65 0.32568231986243035 1107 66-67 0.326124850025181 1107 68-69 0.3633870957298839 1107 70-71 0.4766926558196971 1107 72-73 0.34320292914931017 1107 74-75 0.37902702100028307 1107 76-77 0.252268695593564 1107 78-79 0.468247026156142 1107 80-81 0.6680427673109754 1107 82-83 0.7411654438209823 1107 84-85 0.9582581207148415 1107 86-87 0.8506456505356468 1107 88-89 0.6569722489387431 1107 90-91 0.672713111748326 1107 92-93 1.0010145163755944 1107 94-95 1.1090019404931617 1107 96-97 0.9702982904754371 1107 98-99 1.1785218326688174 1107 100-101 1.411276846092786 1108 1 0.025647315098517254 1108 2 0.06696979041261386 1108 3 0.0260543274002778 1108 4 0.0650726428671149 1108 5 -0.132998282963797 1108 6 -0.0033681382007983984 1108 7 0.07929859105856707 1108 8 0.07253649236358228 1108 9 0.07026235538393166 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-1.9958862538961917 2316 80-81 -1.9477802537801203 2316 82-83 -1.7522804577928248 2316 84-85 -2.101138228595655 2316 86-87 -2.226558785935037 2316 88-89 -2.4521315497174854 2316 90-91 -2.7105230129642557 2316 92-93 -2.66120288665266 2316 94-95 -2.7054868490806356 2316 96-97 -3.0280945183418204 2316 98-99 -3.238271465606786 2316 100-101 -3.602200172338808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8257.0 3 1669.0 4 1588.0 5 1819.0 6 1940.0 7 2273.0 8 2920.0 9 3643.0 10 4550.0 11 5972.0 12 7434.0 13 9333.0 14 10933.0 15 13174.0 16 15740.0 17 19056.0 18 16372.0 19 13579.0 20 13258.0 21 14259.0 22 15903.0 23 17474.0 24 20125.0 25 22958.0 26 26809.0 27 31697.0 28 38077.0 29 46005.0 30 56864.0 31 70588.0 32 90763.0 33 118528.0 34 160964.0 35 225090.0 36 324544.0 37 432375.0 38 321947.0 39 37460.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58191004374095 19.052527442315363 15.184169429114206 23.181393084829487 2 14.922280679781624 19.713613022570183 41.013824677179414 24.350281620468774 3 19.087181792830783 23.81858964626954 29.649900596511035 27.444327964388638 4 12.563603515932979 16.477264023860048 37.563400777614085 33.39573168259289 5 13.73807055554279 37.22405662608752 34.49119283406612 14.546679984303571 6 32.171375499662126 36.423799021362285 17.34945758043553 14.055367898540059 7 28.727165838325064 32.382612343782846 20.518954025353057 18.37126779253903 8 26.30476511166852 35.283938568359716 19.579399620779007 18.83189669919276 9 25.817892578984846 17.14772257688717 20.150105649240917 36.884279194887064 10-11 24.757372584447126 26.72682441341357 27.506748498851557 21.009054503287746 12-13 25.707092943791814 23.72210952433689 27.97686737094591 22.593930160925378 14-15 22.4648822679002 25.425285307306744 25.991927237549127 26.117905187243927 16-17 22.14324734405144 28.663896768745033 26.76077927181801 22.432076615385515 18-19 22.12251488463996 27.288367799886696 28.902276230738533 21.686841084734805 20-21 22.59567040174862 26.405044386169124 29.25575633834135 21.743528873740907 22-23 22.31126009356366 26.195304351975096 29.204321771488782 22.289113782972457 24-25 22.179021601585557 26.46384954211768 29.165099809636718 22.192029046660046 26-27 22.01681356859847 26.764364740475756 28.90645640854145 22.312365282384324 28-29 21.973316623586854 27.059885894759205 28.90250457132208 22.06429291033186 30-31 22.20416910057412 26.68204108323232 28.803890650306307 22.309899165887252 32-33 21.922904386881246 26.68684777443977 28.8293404406126 22.560907398066384 34-35 22.364385181156663 26.914877955554633 28.610853867663366 22.109882995625334 36-37 22.43143964149846 26.473696624014494 28.720812785930722 22.374050948556327 38-39 22.07106925259625 26.844788410848807 28.65039447909315 22.433747857461796 40-41 22.433506026636973 26.68299455184546 28.690838328056362 22.1926610934612 42-43 22.209486196253433 26.663019156853572 28.51516942465522 22.612325222237775 44-45 22.26102918835023 26.9811846482863 28.504094237458123 22.253691925905354 46-47 22.619675158263675 27.066498428279534 27.948827140459098 22.364999272997693 48-49 22.411895339095405 27.224107248570323 27.80439624059169 22.55960117174258 50-51 22.455831371020984 27.40749894821859 27.806421797998095 22.330247882762333 52-53 22.72232991347927 27.171427220597472 27.73506149658577 22.371181369337485 54-55 22.435011220679364 27.30879264589697 27.811956269737482 22.444239863686192 56-57 22.43468136080067 27.436416410009905 27.742401677169227 22.3865005520202 58-59 22.438973672057923 27.098664743854606 27.830319037369712 22.632042546717763 60-61 22.251661709536073 27.332238616471116 27.994424550162055 22.42167512383075 62-63 22.267772844627366 27.32974329613666 28.10093063702455 22.30155322221142 64-65 22.088724230530605 27.34846508940002 28.009178880832753 22.553631799236623 66-67 22.150077994975558 27.377091530006687 27.84152843148639 22.631302043531367 68-69 22.45294185154265 27.11026402068421 27.98445984108804 22.452334286685105 70-71 22.348555347134155 27.211798026535654 27.745009232680424 22.69463739364976 72-73 22.275725056294622 27.290346130355076 27.733726769325497 22.700202044024802 74-75 22.26725312870777 27.585651244731473 27.622830249486423 22.524265377074325 76-77 22.39200863323277 27.276851975842252 27.720756780722418 22.610382610202564 78-79 22.355675693436556 27.425967381380033 27.669383872262156 22.548973052921255 80-81 22.436331391842135 27.441552712836348 27.476751618159394 22.645364277162123 82-83 22.49367464910984 27.40779097795562 27.59169480246654 22.506839570468 84-85 22.382884077959293 27.56719365765591 27.485396303119618 22.564525961265183 86-87 22.49473778484257 27.524839410739915 27.447228206643377 22.533194597774138 88-89 22.46109449386612 27.52088057096514 27.288649196506448 22.729375738662295 90-91 22.315529467733874 27.541827918345007 27.434730624087596 22.70791198983352 92-93 22.479000815281662 27.257388447843688 27.61952832479309 22.64408241208156 94-95 22.279030582004534 27.117277147137724 27.650762176118466 22.952930094739273 96-97 22.458458932375887 26.984142342681295 27.680918876444743 22.87647984849808 98-99 22.262707687737564 27.20789815396179 27.42785643075275 23.101537727547893 100-101 22.382091302691485 26.853235098681317 27.846697597890934 22.917976000736264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2894.0 1 1606.0 2 326.0 3 323.5 4 272.0 5 264.5 6 410.5 7 797.0 8 1358.0 9 1654.0 10 1514.5 11 1366.5 12 1442.5 13 1580.5 14 1727.5 15 1890.5 16 2045.5 17 2227.5 18 2428.5 19 2733.5 20 3140.5 21 3654.5 22 4436.5 23 5559.5 24 6931.5 25 8706.5 26 11333.0 27 14912.5 28 17936.0 29 20846.5 30 24840.5 31 30226.5 32 37559.0 33 42177.5 34 44457.5 35 48132.5 36 53255.5 37 58617.5 38 62863.0 39 66562.0 40 69700.0 41 73567.0 42 75931.5 43 76752.0 44 79392.0 45 83171.0 46 84458.0 47 85156.5 48 86002.5 49 84309.5 50 81720.0 51 79349.5 52 77698.0 53 76576.5 54 75475.5 55 70303.0 56 63335.5 57 57215.5 58 51733.0 59 45212.0 60 36398.5 61 29680.5 62 24402.0 63 18145.0 64 12615.0 65 9111.5 66 6585.5 67 4942.0 68 3636.0 69 2652.0 70 2062.5 71 1475.0 72 1028.0 73 739.5 74 509.5 75 367.0 76 260.5 77 171.5 78 124.5 79 87.5 80 54.0 81 39.0 82 27.5 83 23.0 84 18.5 85 20.5 86 20.5 87 17.5 88 16.0 89 12.0 90 9.5 91 7.0 92 5.5 93 4.0 94 6.5 95 7.5 96 7.0 97 10.5 98 8.5 99 6.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32332378831074665 2 0.2824871447683722 3 0.2785786871356037 4 0.2844638359849448 5 0.2843290615838148 6 0.21074223856686292 7 0.19924148967044064 8 0.20422814251224874 9 0.19946611367232392 10-11 0.2197945858427578 12-13 0.20952926895669338 14-15 0.20312748490302082 16-17 0.2048795521177102 18-19 0.19948857607251222 20-21 0.19335634082109954 22-23 0.19555765603955538 24-25 0.1858763615583874 26-27 0.18711179356874527 28-29 0.2014428048888965 30-31 0.21202259537759743 32-33 0.20474477771658023 34-35 0.21307832818644867 36-37 0.1909304016007605 38-39 0.19218829601130666 40-41 0.18780812797458335 42-43 0.1947490096327757 44-45 0.19789374565914114 46-47 0.20175727849153302 48-49 0.20822644974577056 50-51 0.21161827217420762 52-53 0.2285324595160161 54-55 0.20651930733145787 56-57 0.18432645594539301 58-59 0.18850446238042143 60-61 0.1823273023286321 62-63 0.1903913039962407 64-65 0.1843039935452047 66-67 0.1798789007081047 68-69 0.17776743509040216 70-71 0.18933557118738942 72-73 0.1863929967627189 74-75 0.19128980000377369 76-77 0.1929295552175214 78-79 0.19027899199529907 80-81 0.19236799521281328 82-83 0.18547203835499756 84-85 0.17913764150189 86-87 0.17830653269492197 88-89 0.1805976975141311 90-91 0.18556188795575088 92-93 0.18017091191055293 94-95 0.17740803668738897 96-97 0.17628491667797272 98-99 0.18675239516573208 100-101 0.1759030558747712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2225942.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.184306253447616 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56249471314808 43.20988838951177 2 13.691472177313823 15.658746760961417 3 4.706864154482728 8.074762839099453 4 2.090492574669244 4.78173470441777 5 1.0974126226605752 3.1377389750310734 6 0.6865884154469063 2.3557249331391126 7 0.42541359855431965 1.7028887052878003 8 0.311753040833376 1.4261905089967468 9 0.21729077597766847 1.1183060051600646 >10 1.1221777564082507 11.187090364825217 >50 0.058155158545152866 2.24489111783314 >100 0.026349580226405107 2.6600331016595677 >500 0.0022783893394016977 0.9332672368643257 >1k 0.0011784772445179318 1.2399834274034778 >5k 7.85651496345288E-5 0.26875292980897114 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5982 0.26874015585311745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4469 0.20076893288324674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3137 0.14092909878154958 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3078 0.13827853555932723 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.4924800376649525E-5 0.0 5 0.0 0.0 0.0 4.4924800376649525E-5 0.0 6 0.0 0.0 0.0 4.4924800376649525E-5 0.0 7 0.0 0.0 0.0 4.4924800376649525E-5 0.0 8 0.0 0.0 0.0 4.4924800376649525E-5 0.0 9 0.0 0.0 0.0 4.4924800376649525E-5 0.0 10-11 0.0 0.0 0.0 4.4924800376649525E-5 0.0 12-13 0.0 0.0 0.0 8.984960075329905E-5 2.2462400188324762E-5 14-15 0.0 0.0 0.0 8.984960075329905E-5 6.73872005649743E-5 16-17 0.0 0.0 0.0 1.5723680131827334E-4 8.984960075329905E-5 18-19 0.0 0.0 0.0 2.2462400188324764E-4 8.984960075329905E-5 20-21 0.0 0.0 0.0 3.144736026365467E-4 8.984960075329905E-5 22-23 0.0 0.0 0.0 4.043232033898457E-4 8.984960075329905E-5 24-25 0.0 0.0 0.0 5.615600047081191E-4 8.984960075329905E-5 26-27 0.0 0.0 0.0 7.187968060263924E-4 8.984960075329905E-5 28-29 0.0 0.0 0.0 0.0012129696101695372 8.984960075329905E-5 30-31 0.0 0.0 0.0 0.0028078000235405957 8.984960075329905E-5 32-33 4.4924800376649525E-5 0.0 0.0 0.005188814443503021 1.347744011299486E-4 34-35 8.984960075329905E-5 0.0 0.0 0.007390129661958848 1.347744011299486E-4 36-37 8.984960075329905E-5 0.0 0.0 0.010512403288135989 1.347744011299486E-4 38-39 8.984960075329905E-5 0.0 0.0 0.013657139314501457 1.5723680131827334E-4 40-41 8.984960075329905E-5 0.0 0.0 0.016891724941620223 1.796992015065981E-4 42-43 1.347744011299486E-4 0.0 0.0 0.02214792658568822 1.796992015065981E-4 44-45 1.796992015065981E-4 0.0 0.0 0.028033075435029305 1.796992015065981E-4 46-47 1.796992015065981E-4 0.0 0.0 0.03760205791525566 1.796992015065981E-4 48-49 1.796992015065981E-4 0.0 0.0 0.051146885228815483 2.2462400188324764E-4 50-51 1.796992015065981E-4 0.0 0.0 0.06282733332674437 2.2462400188324764E-4 52-53 1.796992015065981E-4 0.0 0.0 0.08371736550188638 2.695488022598972E-4 54-55 1.796992015065981E-4 0.0 0.0 0.11709649218173698 3.144736026365467E-4 56-57 1.796992015065981E-4 0.0 0.0 0.16804121580885756 3.144736026365467E-4 58-59 1.796992015065981E-4 0.0 0.0 0.21458330899906647 3.144736026365467E-4 60-61 1.796992015065981E-4 0.0 0.0 0.2657301942278819 3.3693600282487144E-4 62-63 1.796992015065981E-4 0.0 0.0 0.3255475659293908 3.593984030131962E-4 64-65 1.796992015065981E-4 0.0 0.0 0.3976743329341016 3.593984030131962E-4 66-67 1.796992015065981E-4 0.0 0.0 0.49554301055463257 3.593984030131962E-4 68-69 1.796992015065981E-4 0.0 0.0 0.618412339584769 3.593984030131962E-4 70-71 1.796992015065981E-4 0.0 0.0 0.7424047886243217 3.593984030131962E-4 72-73 1.796992015065981E-4 0.0 0.0 0.8848388682184891 3.593984030131962E-4 74-75 1.796992015065981E-4 0.0 0.0 1.034101517469907 3.593984030131962E-4 76-77 1.796992015065981E-4 0.0 0.0 1.203602789291006 3.593984030131962E-4 78-79 1.796992015065981E-4 0.0 0.0 1.3748785907269823 3.8186080320152095E-4 80-81 2.2462400188324764E-4 0.0 0.0 1.5711325811723755 4.0432320338984576E-4 82-83 2.2462400188324764E-4 0.0 0.0 1.792701696630011 4.0432320338984576E-4 84-85 2.470864020715724E-4 0.0 0.0 2.050053415587648 4.0432320338984576E-4 86-87 2.695488022598972E-4 0.0 0.0 2.326296013103666 4.0432320338984576E-4 88-89 2.695488022598972E-4 0.0 0.0 2.6445208365716626 4.0432320338984576E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 46.76562 1 GTATCAA 6480 0.0 42.04794 1 ATCAACG 8045 0.0 33.264503 3 TATCAAC 8155 0.0 33.049706 2 TCAACGC 8165 0.0 32.77636 4 CAACGCA 8225 0.0 32.594322 5 AACGCAG 8575 0.0 31.188837 6 ACGCAGA 9835 0.0 27.144203 7 CGCAGAG 9915 0.0 26.780853 8 GTACATG 7370 0.0 23.9371 1 TACATGG 7150 0.0 23.06917 2 GCAGAGT 11205 0.0 22.97697 9 ACATGGG 7195 0.0 21.801275 3 GAGTACT 6290 0.0 21.597141 12-13 GTATAGG 530 0.0 21.532799 1 AGAGTAC 9650 0.0 20.427092 10-11 GTACTTT 6680 0.0 20.122686 14-15 TAAGGTG 1070 0.0 19.990675 5 CAGAGTA 10360 0.0 19.921215 10-11 CATGGGG 4350 0.0 19.341608 4 >>END_MODULE