FastQCFastQC Report
Thu 26 May 2016
SRR937218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225942
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70070.31478807623918326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.25387004692844645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.17803698389266207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42300.044.834821
GTATCAA72850.040.3219761
ATCAACG93500.031.0426463
TCAACGC94150.030.727424
CAACGCA95550.030.3766355
TATCAAC96250.030.2544252
AACGCAG99500.029.1667986
ACGCAGA112550.025.6606547
CGCAGAG113350.025.3538278
ATGGGCG2501.4551915E-1124.7020195
TAAGGTG12700.022.8168285
GTAAGGT11950.022.6587564
GCAGAGT127750.022.2356539
GTACATG71750.021.3975221
TACATGG70750.021.3515722
AGAGTAC111250.021.30640410-11
GAGTACT78700.020.70279712-13
AGTACTT83150.020.45174612-13
AGGACCG6800.020.259015
ACATGGG70400.020.108183
Produced by FastQC (version 0.11.5)