FastQCFastQC Report
Thu 26 May 2016
SRR937217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370429
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63390.2674199480347228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46230.19502798860459436No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35060.14790571664453986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.13330920268018995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.046.62981
GTATCAA70200.041.7235641
ATCAACG88950.032.5392073
TCAACGC89750.032.197574
TATCAAC90650.031.9814112
CAACGCA91750.031.3383225
AACGCAG95750.030.2129886
ACGCAGA109850.026.163087
CGCAGAG111300.025.7368668
GTACATG76350.024.288091
TACATGG76750.023.5310252
ACATGGG77050.022.144073
GCAGAGT126650.022.1299489
GTGTAGC15650.021.5715731
AGAGTAC106250.021.30386210-11
GAGTACT71100.020.97853912-13
CAGAGTA114700.020.0450110-11
GTACTTT76200.019.47889514-15
AGTACTT77200.019.2901412-13
GTAAGGT12850.019.2333184
Produced by FastQC (version 0.11.5)