##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937217_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2370429 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.574432307400897 31.0 31.0 34.0 30.0 34.0 2 31.678348518348365 33.0 31.0 34.0 30.0 34.0 3 31.733367251244395 33.0 31.0 34.0 30.0 34.0 4 35.38142294074195 37.0 35.0 37.0 33.0 37.0 5 35.35353727110156 37.0 35.0 37.0 33.0 37.0 6 35.42104741378037 37.0 35.0 37.0 33.0 37.0 7 35.35880973444048 37.0 35.0 37.0 33.0 37.0 8 35.336316337675584 37.0 35.0 37.0 33.0 37.0 9 36.945447849313354 39.0 37.0 39.0 33.0 39.0 10-11 36.84553492215966 39.0 37.0 39.0 33.0 39.0 12-13 36.72941564585989 39.0 37.0 39.0 33.0 39.0 14-15 37.8756845279905 40.0 38.0 41.0 33.0 41.0 16-17 37.8016405469221 40.0 38.0 41.0 33.0 41.0 18-19 37.73476151363319 40.0 37.5 41.0 32.0 41.0 20-21 37.647006512323294 40.0 37.0 41.0 32.0 41.0 22-23 37.50895808311491 40.0 37.0 41.0 32.0 41.0 24-25 37.36004917253375 39.5 37.0 41.0 32.0 41.0 26-27 37.187419450234536 39.0 36.5 41.0 31.5 41.0 28-29 36.99906177320645 39.0 36.0 40.5 31.0 41.0 30-31 37.00019743261663 39.0 36.0 41.0 31.0 41.0 32-33 36.86030418966356 39.0 36.0 41.0 30.5 41.0 34-35 36.66553248378247 39.0 36.0 40.5 30.0 41.0 36-37 36.67722783513027 39.0 36.0 41.0 30.0 41.0 38-39 36.64789917774378 39.0 36.0 41.0 30.0 41.0 40-41 36.48627315983731 39.0 35.5 41.0 30.0 41.0 42-43 36.25965996028567 39.0 35.0 41.0 30.0 41.0 44-45 36.05937933597674 39.0 35.0 41.0 29.0 41.0 46-47 35.788280307066785 39.0 35.0 40.0 28.0 41.0 48-49 35.50950039001379 39.0 35.0 40.0 27.0 41.0 50-51 34.60177398268415 38.0 34.0 39.5 25.5 40.5 52-53 34.749079175119775 38.0 34.0 39.5 26.0 40.5 54-55 35.09412895302918 38.0 34.5 40.0 26.0 41.0 56-57 34.85760003779907 38.0 34.0 40.0 26.0 41.0 58-59 34.596140614209496 38.0 34.0 40.0 25.5 41.0 60-61 34.30531287796428 38.0 34.0 40.0 24.5 41.0 62-63 33.97357123963637 37.0 33.5 40.0 23.5 41.0 64-65 33.60136013354545 37.0 33.0 39.5 23.0 41.0 66-67 33.21159777407381 36.0 32.5 39.0 22.0 41.0 68-69 32.695469680804614 35.5 32.0 39.0 20.5 40.0 70-71 32.2643755623982 35.0 31.5 38.5 19.5 40.0 72-73 31.838746910369387 35.0 31.0 37.5 18.0 40.0 74-75 31.37035173801873 35.0 31.0 37.0 17.0 39.0 76-77 30.929064527982064 35.0 30.5 36.5 12.5 39.0 78-79 30.45449452398701 34.0 30.0 36.0 8.0 38.5 80-81 30.003447266296526 34.0 30.0 35.5 7.0 37.0 82-83 29.538636466226155 34.0 29.0 35.0 2.0 37.0 84-85 29.06333410534549 34.0 29.0 35.0 2.0 36.5 86-87 28.61329805701837 34.0 29.0 35.0 2.0 36.0 88-89 28.19161847918668 34.0 27.5 35.0 2.0 36.0 90-91 27.735890634142596 33.0 27.0 35.0 2.0 35.0 92-93 27.257424289021102 33.0 26.0 35.0 2.0 35.0 94-95 26.66792951824332 33.0 25.0 35.0 2.0 35.0 96-97 25.981506723044646 32.0 24.5 34.5 2.0 35.0 98-99 25.197636799077298 32.0 20.5 34.0 2.0 35.0 100-101 23.290076184521872 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10811741940391784 1101 2 0.14804086975396302 1101 3 0.1767724053005928 1101 4 0.18907104687844623 1101 5 0.21731233712844755 1101 6 0.11578084132130328 1101 7 0.20284020729647523 1101 8 0.132495689637679 1101 9 0.12881664600686804 1101 10-11 0.15757333357035463 1101 12-13 0.2392278080811323 1101 14-15 0.2978381667878338 1101 16-17 0.3094545142053775 1101 18-19 0.286487576404717 1101 20-21 0.31522705871260825 1101 22-23 0.13110503521716765 1101 24-25 0.3905650127185254 1101 26-27 0.41723068295235777 1101 28-29 0.3919981496249605 1101 30-31 0.4037087619268078 1101 32-33 0.3196427608204786 1101 34-35 0.19790022085434344 1101 36-37 0.3481914830869073 1101 38-39 0.4198699820713472 1101 40-41 0.48486531057944404 1101 42-43 0.47148017558198063 1101 44-45 0.2872486294689409 1101 46-47 0.5600384673227623 1101 48-49 0.5816233772250641 1101 50-51 0.5133174323753664 1101 52-53 0.48035276638172064 1101 54-55 0.5954541565499554 1101 56-57 0.672236764855171 1101 58-59 0.5565051299966584 1101 60-61 0.6405414692810112 1101 62-63 0.6815298059753943 1101 64-65 0.5506145182751041 1101 66-67 0.6307792610527443 1101 68-69 0.5113860103384482 1101 70-71 0.5353024812233969 1101 72-73 0.7171977465340049 1101 74-75 0.7057811716963265 1101 76-77 0.731102494051683 1101 78-79 0.781565812539732 1101 80-81 0.7872510069075389 1101 82-83 0.8766362454129037 1101 84-85 0.8527182584584274 1101 86-87 0.980444753734222 1101 88-89 0.8654359437736296 1101 90-91 0.7948746184543403 1101 92-93 0.8263777060261184 1101 94-95 0.8947479502802267 1101 96-97 0.988870880780869 1101 98-99 1.1442169914251004 1101 100-101 1.6769275183769032 1102 1 0.18191115851354311 1102 2 0.09264812451955251 1102 3 0.0765155233316861 1102 4 -0.005871799687859891 1102 5 0.1419602929163304 1102 6 0.007688965821188276 1102 7 0.06880820735630522 1102 8 0.16849702452650916 1102 9 0.24234015425924582 1102 10-11 0.09909155461598118 1102 12-13 0.3110582416769532 1102 14-15 0.40998658997376225 1102 16-17 0.35198773685885243 1102 18-19 0.4297495193597243 1102 20-21 0.3691640927167512 1102 22-23 0.35963744272843456 1102 24-25 0.5026010266731618 1102 26-27 0.3808339293122671 1102 28-29 0.2785160061001122 1102 30-31 0.3129169241924572 1102 32-33 0.2411121761616002 1102 34-35 0.33948163411648125 1102 36-37 0.4518311584509149 1102 38-39 0.4176680698524393 1102 40-41 0.2771599265278155 1102 42-43 0.259554888225658 1102 44-45 0.1841639892906528 1102 46-47 0.21854716840487498 1102 48-49 0.29538974550481356 1102 50-51 0.37712955892433087 1102 52-53 0.32841778518981357 1102 54-55 0.36333984407379916 1102 56-57 0.34067697342688064 1102 58-59 0.3326048708711724 1102 60-61 0.3582907700535287 1102 62-63 0.39663854145801025 1102 64-65 0.3866029062379255 1102 66-67 0.2858614670219737 1102 68-69 0.31888064460881793 1102 70-71 0.2177155545338465 1102 72-73 0.13886183316342837 1102 74-75 0.19867087884340506 1102 76-77 0.25133475349125334 1102 78-79 0.5094962113611388 1102 80-81 0.483813154022009 1102 82-83 0.453613198949796 1102 84-85 0.4078548695608113 1102 86-87 0.3827344900469356 1102 88-89 0.4605276098794171 1102 90-91 0.41343971578531225 1102 92-93 0.21687031552813707 1102 94-95 0.10544701884938945 1102 96-97 0.2730012212709987 1102 98-99 0.49093777343813017 1102 100-101 0.6285892119283041 1103 1 0.07230138659699037 1103 2 0.039275516152891043 1103 3 0.09712590779462715 1103 4 0.030908007900507073 1103 5 -0.1343837059306381 1103 6 0.0012437166589336357 1103 7 0.07019544477207518 1103 8 0.03737606463027987 1103 9 0.1501564263739752 1103 10-11 0.10213401849375714 1103 12-13 0.1447113602587322 1103 14-15 0.1668372007185468 1103 16-17 0.19096685623333798 1103 18-19 0.09069157967752517 1103 20-21 0.08566546193809899 1103 22-23 0.11928054437510838 1103 24-25 0.10288523018269302 1103 26-27 -0.040577719878811536 1103 28-29 -0.1879853631212356 1103 30-31 -0.06247942935490869 1103 32-33 -0.13626369311378284 1103 34-35 -0.071079836715505 1103 36-37 -0.026292828145130898 1103 38-39 -0.0013879781423682402 1103 40-41 0.06253222862128638 1103 42-43 -0.031001538106636417 1103 44-45 -0.1693090586681265 1103 46-47 -0.0906806628205743 1103 48-49 -0.1488617768531313 1103 50-51 -0.11791827374241137 1103 52-53 -0.12555565912251154 1103 54-55 -0.15120588071271612 1103 56-57 -0.028069198158171105 1103 58-59 -0.09795443803236736 1103 60-61 -0.08578059160106477 1103 62-63 -0.038778350409529594 1103 64-65 0.075019473816198 1103 66-67 0.028400561894606824 1103 68-69 0.05776472921228759 1103 70-71 -0.039686798364940046 1103 72-73 0.018793732417563547 1103 74-75 0.20875762622207006 1103 76-77 0.18362585715572521 1103 78-79 0.2612167460781123 1103 80-81 0.08272588417730731 1103 82-83 0.07517888554223617 1103 84-85 0.04089228893889896 1103 86-87 0.23367628012368158 1103 88-89 0.32199979306681925 1103 90-91 0.469544010816481 1103 92-93 0.39783048199662474 1103 94-95 0.5505407924954824 1103 96-97 0.5374343456179176 1103 98-99 0.6987734128284657 1103 100-101 1.0220939681708678 1104 1 -0.023515298734999135 1104 2 -0.0046201895185973285 1104 3 0.04021052773114775 1104 4 0.021138055973906944 1104 5 0.06508115354147748 1104 6 0.041386591966421804 1104 7 0.038536937052455755 1104 8 0.07112158373004007 1104 9 0.03624558467384986 1104 10-11 0.031226675930469128 1104 12-13 0.11938968908467729 1104 14-15 0.13235994388532646 1104 16-17 0.15395480365091174 1104 18-19 0.053871172691962954 1104 20-21 0.15684055815824394 1104 22-23 0.1385386344479258 1104 24-25 0.014240124817348487 1104 26-27 0.15121367434748834 1104 28-29 0.07597053210516691 1104 30-31 -0.041896019811630936 1104 32-33 -0.0415831268942739 1104 34-35 0.15544984359396352 1104 36-37 -0.0480487297291603 1104 38-39 -0.030354583515048716 1104 40-41 -0.08240655482347137 1104 42-43 -0.03245248172431303 1104 44-45 -0.045664026659949286 1104 46-47 -2.532839699327383E-4 1104 48-49 0.10980384406637711 1104 50-51 0.06293373306512962 1104 52-53 0.15198078716292684 1104 54-55 0.22291475025672725 1104 56-57 0.06480617046158699 1104 58-59 0.033902928738790195 1104 60-61 0.04462308065867404 1104 62-63 0.12105163213146142 1104 64-65 0.1882719349491282 1104 66-67 0.1407534807762545 1104 68-69 0.07530809552374507 1104 70-71 0.0634102800636498 1104 72-73 0.12152005150167255 1104 74-75 0.008819307372700536 1104 76-77 -0.018858872327498943 1104 78-79 0.05308412610246194 1104 80-81 0.30845994488770145 1104 82-83 0.3347883686676454 1104 84-85 0.31812399732949004 1104 86-87 0.26656688258163896 1104 88-89 0.18237329107884293 1104 90-91 0.34007380849741864 1104 92-93 0.23837798320701964 1104 94-95 0.4003869258392463 1104 96-97 0.2981863666078475 1104 98-99 0.39755941285780594 1104 100-101 0.5339524597519123 1105 1 0.15938201291838539 1105 2 0.12384093027422338 1105 3 0.08556343977047831 1105 4 0.06745881512051 1105 5 0.11587824689193837 1105 6 0.12867122366538553 1105 7 0.09274298829578242 1105 8 0.16963416772640727 1105 9 0.19304210664070354 1105 10-11 0.16183941209739316 1105 12-13 0.19882961742364813 1105 14-15 0.2077139669483259 1105 16-17 0.25047258763485303 1105 18-19 0.24068988537264602 1105 20-21 0.25093186314152405 1105 22-23 0.34505048966567387 1105 24-25 0.35767919938754034 1105 26-27 0.34437158718371563 1105 28-29 0.31616178028074415 1105 30-31 0.2200646790487113 1105 32-33 0.1806302041993817 1105 34-35 0.3706092490375852 1105 36-37 0.4034073064690489 1105 38-39 0.3680370113263578 1105 40-41 0.3177338276464283 1105 42-43 0.2202702702548649 1105 44-45 0.2469516669461811 1105 46-47 0.3192328146770578 1105 48-49 0.28833972649362494 1105 50-51 0.20985206422822245 1105 52-53 0.24089673888366292 1105 54-55 0.3990328335557294 1105 56-57 0.42517504685292806 1105 58-59 0.3970334925370125 1105 60-61 0.41020930436197034 1105 62-63 0.4951630611922724 1105 64-65 0.33258952833959654 1105 66-67 0.5869328985337177 1105 68-69 0.717600468015597 1105 70-71 0.5083280576988756 1105 72-73 0.5120527245254252 1105 74-75 0.4110989063989656 1105 76-77 0.5924139507770292 1105 78-79 0.8267757138677112 1105 80-81 0.673903240025183 1105 82-83 0.6130737582434662 1105 84-85 0.6423948549802461 1105 86-87 0.36595406705587763 1105 88-89 0.5384210789844239 1105 90-91 0.6450805573048832 1105 92-93 0.6269666954710651 1105 94-95 0.7002313825255264 1105 96-97 0.7468041671910015 1105 98-99 0.7290422525020439 1105 100-101 0.9287564566595634 1106 1 -0.025586009066749682 1106 2 0.05007954186184804 1106 3 0.033224614115699325 1106 4 -0.08775770172555752 1106 5 -0.016282141043710396 1106 6 -0.11315071102102081 1106 7 0.019223267309406822 1106 8 -0.15898466991929894 1106 9 0.004686778930434343 1106 10-11 -0.21924040550312895 1106 12-13 -0.35797543355761974 1106 14-15 -0.20358616418596398 1106 16-17 -0.3560155884891998 1106 18-19 -0.25384948726928513 1106 20-21 -0.1816112309146476 1106 22-23 -0.0428323320062205 1106 24-25 -0.1741231774049936 1106 26-27 -0.01169499153185427 1106 28-29 0.1460048021008049 1106 30-31 0.17195767452305688 1106 32-33 0.25608920723540507 1106 34-35 0.253770311793204 1106 36-37 -0.09269432062622229 1106 38-39 -0.1128712620193042 1106 40-41 -0.010972168743087707 1106 42-43 -0.03405498240645244 1106 44-45 0.04106818762194564 1106 46-47 0.09534445182200812 1106 48-49 0.05688704114593435 1106 50-51 0.0473247775987673 1106 52-53 0.15236854633150188 1106 54-55 0.2838439698020139 1106 56-57 0.1912456005354457 1106 58-59 0.011706667056550657 1106 60-61 0.05383965092362786 1106 62-63 0.2393983686711536 1106 64-65 0.2885935112300757 1106 66-67 0.19168384242328784 1106 68-69 0.2639187374127019 1106 70-71 0.07094303130695323 1106 72-73 0.21726072358517 1106 74-75 0.297459751521842 1106 76-77 0.2213468240178429 1106 78-79 0.14056964154444174 1106 80-81 0.2010855431025469 1106 82-83 0.24366991084522027 1106 84-85 0.38443402623265044 1106 86-87 0.4016554770466456 1106 88-89 0.5777006195257854 1106 90-91 0.5223102034271783 1106 92-93 0.5772564982942185 1106 94-95 0.48485420306170823 1106 96-97 0.8136991633546948 1106 98-99 0.9474706482925406 1106 100-101 0.9980034183189623 1107 1 -0.038241091681886275 1107 2 -0.04749518347431092 1107 3 0.026472816721891945 1107 4 -0.019628436023459983 1107 5 0.15657639573289828 1107 6 0.17509386521656722 1107 7 -0.014591077138256026 1107 8 0.20471051619435343 1107 9 0.16479549012846917 1107 10-11 0.1330150215262762 1107 12-13 0.17014897883635882 1107 14-15 0.32276326964315416 1107 16-17 0.29319259991643776 1107 18-19 0.28708745892905796 1107 20-21 0.21762254898110456 1107 22-23 0.2848673342517074 1107 24-25 0.21349578672408853 1107 26-27 0.23126453543576986 1107 28-29 0.2907975509608036 1107 30-31 0.2684889567619848 1107 32-33 0.17993746312998127 1107 34-35 0.3354519050141036 1107 36-37 0.3311097400354086 1107 38-39 0.2737968239768165 1107 40-41 0.4060036830542302 1107 42-43 0.3475337373003029 1107 44-45 0.34446927850913056 1107 46-47 0.33993448341025356 1107 48-49 0.36222219528655586 1107 50-51 0.21805050869863862 1107 52-53 0.1754793861607169 1107 54-55 0.29263147041003634 1107 56-57 0.3123588702857276 1107 58-59 0.24842168893454897 1107 60-61 0.36713194349280087 1107 62-63 0.4872409697539055 1107 64-65 0.4998450911869625 1107 66-67 0.41681517577259797 1107 68-69 0.3930651940621672 1107 70-71 0.42795557150010666 1107 72-73 0.558658189555743 1107 74-75 0.5653435748686846 1107 76-77 0.41680573207070637 1107 78-79 0.48801132743195197 1107 80-81 0.5621911789657723 1107 82-83 0.6534187076821851 1107 84-85 0.6531744384988514 1107 86-87 0.7617095023157923 1107 88-89 0.7180110461159579 1107 90-91 0.8887990364624905 1107 92-93 0.8384103042388489 1107 94-95 0.9219876185782141 1107 96-97 1.056982395447367 1107 98-99 1.0517937225238327 1107 100-101 1.3656734528983279 1108 1 -0.08876567345587105 1108 2 -0.12369516428229232 1108 3 -0.10183284812153204 1108 4 -0.2162128968877397 1108 5 -0.09499990852453521 1108 6 0.018438074025702633 1108 7 -0.07574099877086127 1108 8 0.003943155416436639 1108 9 0.020030112525553534 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-0.9565679300896051 2316 54-55 -1.0083976816280042 2316 56-57 -1.1325725341178057 2316 58-59 -1.19513207369193 2316 60-61 -1.1530603176835754 2316 62-63 -1.1696330433839037 2316 64-65 -1.102676953857177 2316 66-67 -1.3331728292173253 2316 68-69 -1.213005391318113 2316 70-71 -1.171827051160271 2316 72-73 -1.403653587918381 2316 74-75 -1.744340918696043 2316 76-77 -1.4000895469312482 2316 78-79 -1.2935248427774368 2316 80-81 -1.1626620259026552 2316 82-83 -1.2370948056384208 2316 84-85 -1.5166729215283006 2316 86-87 -1.6304703260822073 2316 88-89 -1.6278801375318075 2316 90-91 -1.7205187270173248 2316 92-93 -1.746436151701598 2316 94-95 -1.861759301380772 2316 96-97 -1.766801191572167 2316 98-99 -2.0926589124527446 2316 100-101 -1.993809465017737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9205.0 3 1716.0 4 1582.0 5 1786.0 6 2193.0 7 2474.0 8 3254.0 9 3956.0 10 5156.0 11 6490.0 12 8302.0 13 10049.0 14 11951.0 15 14318.0 16 17110.0 17 20422.0 18 17694.0 19 14350.0 20 14786.0 21 15620.0 22 17195.0 23 19537.0 24 22293.0 25 25228.0 26 29180.0 27 34821.0 28 42546.0 29 50593.0 30 62154.0 31 77799.0 32 98409.0 33 127839.0 34 171697.0 35 239023.0 36 341182.0 37 450689.0 38 336752.0 39 41076.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60967968415738 18.991649840940283 15.219752944308151 23.178917530594184 2 14.968662017264975 19.677630201227288 40.96080583988543 24.3929019416223 3 19.11127996026592 23.77817282463107 29.60303150042687 27.50751571467614 4 12.596442790921209 16.479919042419386 37.48078759338615 33.442850573273255 5 13.833127678988019 37.18652165250493 34.42653740547267 14.553813263034376 6 32.22815713545905 36.40905103626121 17.344000676396917 14.018791151882814 7 28.76234936397182 32.318002252564575 20.50683548499474 18.412812898468864 8 26.298518189697084 35.28585621578202 19.592280212230747 18.823345382290146 9 25.827214314129428 17.120331574815502 20.098002949980955 36.95445116107412 10-11 24.802146794743194 26.726655943121948 27.46982761080932 21.001369651325543 12-13 25.680060459683858 23.68398188323459 27.99934466776241 22.63661298931914 14-15 22.47650119980157 25.393119164930493 25.945325909986032 26.185053725281897 16-17 22.158879042029582 28.691381428584716 26.746624366791327 22.40311516259437 18-19 22.195607305868513 27.20369710849001 28.865874137255048 21.734821448386423 20-21 22.655349856214787 26.400761781562732 29.189571371048284 21.7543169911742 22-23 22.35489545852974 26.219523665693035 29.145826679650288 22.279754196126937 24-25 22.21844143108346 26.51183974705803 29.0831487555796 22.186570066278914 26-27 22.004532292637887 26.783562417524305 28.845098192980295 22.366807096857517 28-29 22.06195068127148 27.03571866425977 28.84073931589605 22.061591338572693 30-31 22.243098643415482 26.638294651941465 28.77693461745873 22.34167208718433 32-33 21.932147337258844 26.665188293823856 28.792819082256017 22.609845286661287 34-35 22.523154733948314 26.83471859425855 28.513962593093467 22.128164078699676 36-37 22.53107980402978 26.437464054547675 28.647233523851718 22.384222617570824 38-39 22.090751292746987 26.849340951066985 28.602155173598053 22.45775258258797 40-41 22.521606109818133 26.668170055702888 28.622653297071647 22.187570537407332 42-43 22.301427684982762 26.603355837463035 28.48277191362878 22.612444563925425 44-45 22.30775604695346 26.91470301504266 28.491239981635285 22.286300956368592 46-47 22.62937334306438 27.06386833582422 27.901955699224455 22.404802621886947 48-49 22.448000348351847 27.193258546193295 27.80071822203583 22.558022883419024 50-51 22.498149220008802 27.38668174067602 27.752905756295714 22.36226328301946 52-53 22.76177201632849 27.144351210123514 27.68368019784628 22.41019657570172 54-55 22.517838860122577 27.255334428550803 27.782112749333386 22.444713961993234 56-57 22.512382533152348 27.420973581886386 27.66785287921237 22.398791005748894 58-59 22.465422621328017 27.111648592868203 27.775651752393156 22.647277033410624 60-61 22.267616048535047 27.291410824483332 27.950910423060737 22.49006270392088 62-63 22.30107578691194 27.293850478594788 28.049116035619775 22.355957698873492 64-65 22.155142974035765 27.310416124265856 27.946314154040703 22.588126747657675 66-67 22.183495840418846 27.35018131724367 27.78008853878042 22.686234303557065 68-69 22.487024010309366 27.120447150459775 27.92023430217483 22.472294537056037 70-71 22.39431851179635 27.146588194816346 27.714965262088736 22.74412803129857 72-73 22.344779832765436 27.227368791716433 27.715870754960847 22.711980620557284 74-75 22.2779645915021 27.550880879956395 27.55502316403297 22.61613136450853 76-77 22.39012443584525 27.285274559714644 27.644930147639297 22.67967085680081 78-79 22.41633848386414 27.38396193734694 27.626070068270053 22.573629510518863 80-81 22.48641518261972 27.41329819057185 27.45712802178051 22.643158605027917 82-83 22.530590211639538 27.404141652146198 27.539010815741793 22.526257320472467 84-85 22.44875921108521 27.55043775768321 27.41662739562179 22.584175635609785 86-87 22.544182037157142 27.491691069889267 27.376199741460034 22.58792715149356 88-89 22.487070329250887 27.503281687917237 27.21988672232556 22.789761260506314 90-91 22.368141863732504 27.523145760809104 27.345198987605436 22.763513387852957 92-93 22.48664779992518 27.277820870223167 27.53370545479908 22.701825875052574 94-95 22.372640205463064 27.167024970221217 27.568922358733577 22.891412465582146 96-97 22.509718724937102 26.97368573991752 27.59817787776227 22.918417657383113 98-99 22.345141814637266 27.147551558103235 27.40176495279045 23.105541674469055 100-101 22.42479402832169 26.82149797430542 27.810910096014457 22.942797901358436 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3184.0 1 1783.5 2 381.0 3 361.0 4 316.0 5 289.5 6 455.0 7 906.0 8 1432.5 9 1706.5 10 1602.0 11 1478.0 12 1567.5 13 1656.0 14 1754.0 15 1903.0 16 2122.0 17 2324.0 18 2501.5 19 2818.0 20 3285.0 21 3896.5 22 4693.5 23 5835.5 24 7289.5 25 9214.0 26 11879.5 27 15595.5 28 19019.5 29 22090.0 30 25965.5 31 31621.0 32 39454.0 33 44756.5 34 47272.5 35 50748.0 36 56402.5 37 61785.5 38 65731.5 39 69775.0 40 73759.0 41 78144.5 42 80535.5 43 81910.0 44 85014.5 45 88799.5 46 90050.0 47 90939.5 48 91665.0 49 89956.0 50 87486.0 51 84672.5 52 83007.5 53 82339.5 54 80883.0 55 74941.0 56 67627.5 57 61236.0 58 55704.5 59 48685.0 60 39007.5 61 32200.5 62 26523.5 63 19458.5 64 13471.5 65 9788.0 66 7184.0 67 5412.5 68 4077.5 69 3050.5 70 2355.0 71 1584.5 72 1039.5 73 784.5 74 583.5 75 406.5 76 260.5 77 174.0 78 119.0 79 82.0 80 66.0 81 45.5 82 28.5 83 21.5 84 16.5 85 11.5 86 8.0 87 8.5 88 11.0 89 9.5 90 6.5 91 4.0 92 3.0 93 1.5 94 1.0 95 2.0 96 1.5 97 1.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.315554694951842 2 0.28197427554252835 3 0.28328205569540366 4 0.2835773608912142 5 0.27125891558025994 6 0.20899170572077883 7 0.21620558978986504 8 0.2142650127888243 9 0.21051041815637592 10-11 0.2205718880422067 12-13 0.22006565056367436 14-15 0.2037394918810055 16-17 0.2149399960935341 18-19 0.2023473388150415 20-21 0.20762064588308698 22-23 0.2060597469909455 24-25 0.19696856560563508 26-27 0.2092237312317728 28-29 0.21089009626527516 30-31 0.21884224332388777 32-33 0.22004455733540215 34-35 0.20797923076371405 36-37 0.2052582043166026 38-39 0.19547094639831017 40-41 0.1967154468663689 42-43 0.1957873448223929 44-45 0.21190257122233994 46-47 0.20268483046739638 48-49 0.21112212177626918 50-51 0.21979143859613598 52-53 0.22614050030606275 54-55 0.22348275354376781 56-57 0.18964921539518795 58-59 0.1793135335418188 60-61 0.18851018106849013 62-63 0.18730786705697577 64-65 0.185831341077923 66-67 0.1976857353668893 68-69 0.18665397698053812 70-71 0.19279210640774305 72-73 0.2037394918810055 74-75 0.19340381002763635 76-77 0.2038660512506386 78-79 0.20040676181400074 80-81 0.1881726894161352 82-83 0.20262155078257987 84-85 0.18536729005593502 86-87 0.18775082485069158 88-89 0.19614592970302 90-91 0.1920749366464889 92-93 0.19886695615013147 94-95 0.19620920938783654 96-97 0.19011326641717596 98-99 0.19827634575851039 100-101 0.18254079746746263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2370429.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.28162144074017 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0475480069625 42.23797686983638 2 13.829664699229152 15.56711906508766 3 4.8212696593675215 8.140466214967473 4 2.1435578070038943 4.825716361205454 5 1.143241776606188 3.2171750443094353 6 0.707278359067943 2.3884063714973918 7 0.46822157954910504 1.8446588783397697 8 0.33542484188917376 1.510260317842128 9 0.2377253154129014 1.2041609588054523 >10 1.1740981889131261 11.565950895640173 >50 0.06151657224040824 2.3475040150049686 >100 0.026783388599478198 2.6574252523445696 >500 0.0023966074505926437 0.9553226815792 >1k 0.0011983037252949697 1.2706584114093293 >5k 7.48939828309356E-5 0.26719866213049387 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6339 0.2674199480347228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4623 0.19502798860459436 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3506 0.14790571664453986 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3160 0.13330920268018995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.218645654436391E-5 0.0 3 0.0 0.0 0.0 4.218645654436391E-5 0.0 4 0.0 0.0 0.0 4.218645654436391E-5 0.0 5 0.0 0.0 0.0 8.437291308872782E-5 0.0 6 0.0 0.0 0.0 8.437291308872782E-5 0.0 7 0.0 0.0 0.0 8.437291308872782E-5 0.0 8 0.0 0.0 0.0 8.437291308872782E-5 0.0 9 0.0 0.0 0.0 8.437291308872782E-5 0.0 10-11 0.0 0.0 0.0 1.2655936963309173E-4 0.0 12-13 0.0 0.0 0.0 1.2655936963309173E-4 1.2655936963309173E-4 14-15 0.0 0.0 0.0 1.2655936963309173E-4 2.1093228272181955E-4 16-17 0.0 0.0 0.0 1.476525979052737E-4 2.1093228272181955E-4 18-19 0.0 0.0 0.0 1.6874582617745564E-4 2.1093228272181955E-4 20-21 0.0 0.0 0.0 2.5311873926618346E-4 2.1093228272181955E-4 22-23 0.0 0.0 0.0 2.5311873926618346E-4 2.1093228272181955E-4 24-25 4.218645654436391E-5 0.0 0.0 2.5311873926618346E-4 2.1093228272181955E-4 26-27 4.218645654436391E-5 0.0 0.0 4.218645654436391E-4 2.1093228272181955E-4 28-29 1.0546614136090978E-4 0.0 0.0 0.0010968478701534616 2.5311873926618346E-4 30-31 1.2655936963309173E-4 0.0 0.0 0.0028897722732889276 2.5311873926618346E-4 32-33 1.2655936963309173E-4 0.0 0.0 0.0049780018722349415 2.5311873926618346E-4 34-35 1.2655936963309173E-4 0.0 0.0 0.007909960602068233 2.5311873926618346E-4 36-37 1.2655936963309173E-4 0.0 0.0 0.01206532657168808 2.5311873926618346E-4 38-39 1.2655936963309173E-4 0.0 0.0 0.015482429551781555 2.5311873926618346E-4 40-41 1.2655936963309173E-4 0.0 0.0 0.019469049695223943 2.5311873926618346E-4 42-43 1.2655936963309173E-4 0.0 0.0 0.025417340067979258 2.742119675383654E-4 44-45 1.2655936963309173E-4 0.0 0.0 0.03189296114753912 2.9530519581054735E-4 46-47 1.6874582617745564E-4 0.0 0.0 0.0429036263056181 2.9530519581054735E-4 48-49 1.6874582617745564E-4 0.0 0.0 0.056614224682536365 2.9530519581054735E-4 50-51 1.6874582617745564E-4 0.0 0.0 0.07000842463537191 2.9530519581054735E-4 52-53 1.6874582617745564E-4 0.0 0.0 0.09318988250649987 2.9530519581054735E-4 54-55 1.6874582617745564E-4 0.0 0.0 0.12771949718806175 2.9530519581054735E-4 56-57 1.6874582617745564E-4 0.0 0.0 0.1802205423575226 2.9530519581054735E-4 58-59 1.6874582617745564E-4 0.0 0.0 0.2322997229615399 2.9530519581054735E-4 60-61 1.6874582617745564E-4 0.0 0.0 0.28659369253413625 2.9530519581054735E-4 62-63 1.6874582617745564E-4 0.0 0.0 0.35130771687319046 2.9530519581054735E-4 64-65 2.1093228272181955E-4 0.0 0.0 0.42393170181431294 2.9530519581054735E-4 66-67 2.1093228272181955E-4 0.0 0.0 0.5264447912171173 2.9530519581054735E-4 68-69 2.1093228272181955E-4 0.0 0.0 0.647562107955986 2.9530519581054735E-4 70-71 2.3202551099400152E-4 0.0 0.0 0.7727293245231137 2.9530519581054735E-4 72-73 2.5311873926618346E-4 0.0 0.0 0.9116915123802485 3.163984240827293E-4 74-75 2.5311873926618346E-4 0.0 0.0 1.0649760022341948 3.374916523549113E-4 76-77 2.5311873926618346E-4 0.0 0.0 1.2341015065205496 3.374916523549113E-4 78-79 2.5311873926618346E-4 0.0 0.0 1.408964368896938 3.374916523549113E-4 80-81 2.9530519581054735E-4 0.0 0.0 1.5968206598889907 3.374916523549113E-4 82-83 2.9530519581054735E-4 0.0 0.0 1.8158316490390558 3.374916523549113E-4 84-85 2.9530519581054735E-4 0.0 0.0 2.068191032087441 3.374916523549113E-4 86-87 2.9530519581054735E-4 0.0 0.0 2.3324891823378806 3.5858488062709323E-4 88-89 2.9530519581054735E-4 0.0 0.0 2.6376871022080812 3.7967810889927517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4140 0.0 46.6298 1 GTATCAA 7020 0.0 41.723564 1 ATCAACG 8895 0.0 32.539207 3 TCAACGC 8975 0.0 32.19757 4 TATCAAC 9065 0.0 31.981411 2 CAACGCA 9175 0.0 31.338322 5 AACGCAG 9575 0.0 30.212988 6 ACGCAGA 10985 0.0 26.16308 7 CGCAGAG 11130 0.0 25.736866 8 GTACATG 7635 0.0 24.28809 1 TACATGG 7675 0.0 23.531025 2 ACATGGG 7705 0.0 22.14407 3 GCAGAGT 12665 0.0 22.129948 9 GTGTAGC 1565 0.0 21.571573 1 AGAGTAC 10625 0.0 21.303862 10-11 GAGTACT 7110 0.0 20.978539 12-13 CAGAGTA 11470 0.0 20.04501 10-11 GTACTTT 7620 0.0 19.478895 14-15 AGTACTT 7720 0.0 19.29014 12-13 GTAAGGT 1285 0.0 19.233318 4 >>END_MODULE