FastQCFastQC Report
Thu 26 May 2016
SRR937217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370429
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71500.30163316429220194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57530.24269868449972556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.17937681322663535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40850.048.051491
GTATCAA76550.043.0265851
TATCAAC98350.033.424172
ATCAACG98150.033.2018853
TCAACGC99450.032.767874
CAACGCA101050.032.2960435
AACGCAG104350.031.2700826
ACGCAGA119800.027.082187
CGCAGAG120650.026.7338928
TAAGGTG13000.025.5790025
GTAAGGT12500.025.0820394
GCAGAGT133750.023.8668589
AGAGTAC117700.021.69310610-11
GAGTACT81350.021.54708512-13
GGTAAGG14150.021.150133
GTACTTT85300.020.37965414-15
GGACCGA4900.020.3557916
TGGTATC23200.020.2710462
AGTACTT87250.020.06281312-13
CAGAGTA127800.019.58842510-11
Produced by FastQC (version 0.11.5)