FastQCFastQC Report
Thu 26 May 2016
SRR937216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470502
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65020.263185376899108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48430.1960330329625315No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35250.14268355176397346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33800.13681429928006533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.049.9607731
GTATCAA74650.042.4488641
TCAACGC95000.032.906464
ATCAACG95000.032.9017873
TATCAAC96900.032.4037172
CAACGCA97200.032.1629685
AACGCAG101200.031.0757126
ACGCAGA113350.027.5340337
CGCAGAG114650.027.1792938
GCAGAGT129000.023.529879
TAAGGTG13350.023.488715
TACATGG73550.022.4757252
GTACATG78050.022.3998431
GAGTACT75000.022.23007812-13
AGAGTAC109800.022.15011610-11
AGTACTT80850.020.85659812-13
GTACTTT80550.020.81612614-15
CAGAGTA118650.020.69813210-11
ACATGGG76250.020.18473
GTAAGGT14850.018.5558324
Produced by FastQC (version 0.11.5)