##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2470502 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.700696052866988 33.0 31.0 34.0 30.0 34.0 2 31.809257794569685 33.0 31.0 34.0 30.0 34.0 3 31.8628408315395 33.0 31.0 34.0 30.0 34.0 4 35.484346096461366 37.0 35.0 37.0 33.0 37.0 5 35.493963170238274 37.0 35.0 37.0 33.0 37.0 6 35.51884556256178 37.0 35.0 37.0 33.0 37.0 7 35.4118851148471 37.0 35.0 37.0 33.0 37.0 8 35.40805067148296 37.0 35.0 37.0 33.0 37.0 9 37.031295663796264 39.0 37.0 39.0 33.0 39.0 10-11 36.91877845069544 39.0 37.0 39.0 33.0 39.0 12-13 36.769495228095344 39.0 37.0 39.0 33.0 39.0 14-15 37.9436687361516 40.0 38.0 41.0 33.0 41.0 16-17 37.86209543647405 40.0 38.0 41.0 33.0 41.0 18-19 37.799596397817126 40.0 38.0 41.0 33.0 41.0 20-21 37.666991971672154 40.0 37.0 41.0 32.0 41.0 22-23 37.50920784520717 40.0 37.0 41.0 32.0 41.0 24-25 37.44723845598992 40.0 37.0 41.0 32.0 41.0 26-27 37.48345923217225 40.0 37.0 41.0 32.0 41.0 28-29 37.403514751252985 40.0 37.0 41.0 32.0 41.0 30-31 37.19019373390509 39.5 37.0 41.0 31.0 41.0 32-33 36.967291870235286 39.0 36.0 41.0 31.0 41.0 34-35 36.998603320296844 39.0 36.0 41.0 31.0 41.0 36-37 36.8619699559037 39.0 36.0 41.0 30.5 41.0 38-39 36.74513054431853 39.0 36.0 41.0 30.0 41.0 40-41 36.523901822382655 39.0 35.5 41.0 30.0 41.0 42-43 36.267579625517406 39.0 35.0 41.0 30.0 41.0 44-45 36.05957716286002 39.0 35.0 40.5 29.0 41.0 46-47 35.775342825061465 39.0 35.0 40.0 28.0 41.0 48-49 35.47707996188629 39.0 35.0 40.0 27.0 41.0 50-51 34.517975901254076 38.0 34.0 39.5 25.0 40.5 52-53 34.647939366169304 38.0 34.0 39.5 26.0 40.5 54-55 35.01502811979104 38.0 34.0 40.0 26.0 41.0 56-57 34.785346257562225 38.0 34.0 40.0 25.5 41.0 58-59 34.48333779936223 38.0 34.0 40.0 25.0 41.0 60-61 34.156281193053076 38.0 34.0 40.0 23.5 41.0 62-63 33.823991844572475 37.0 33.0 40.0 23.0 41.0 64-65 33.38808145065254 36.5 33.0 39.5 22.0 41.0 66-67 33.01904025983383 36.0 32.0 39.0 21.5 41.0 68-69 32.51652619589055 35.5 32.0 39.0 19.5 40.0 70-71 32.026618679118656 35.0 31.0 38.0 18.0 40.0 72-73 31.55201797043678 35.0 31.0 37.5 16.0 39.5 74-75 31.03988197540419 35.0 30.5 37.0 12.0 39.0 76-77 30.542955642213606 34.0 30.0 36.0 7.5 39.0 78-79 30.026026896557866 34.0 29.5 36.0 6.5 38.5 80-81 29.527550878323513 34.0 29.0 35.5 2.0 37.0 82-83 29.01656222095752 34.0 29.0 35.0 2.0 37.0 84-85 28.462755140453233 34.0 27.5 35.0 2.0 36.0 86-87 27.978729424222283 33.0 26.5 35.0 2.0 36.0 88-89 27.476789737470362 33.0 26.0 35.0 2.0 35.5 90-91 26.922331574716395 32.5 25.5 35.0 2.0 35.0 92-93 26.36035550669459 32.0 25.0 34.5 2.0 35.0 94-95 25.74726594028258 32.0 23.5 34.0 2.0 35.0 96-97 24.935544071609737 32.0 19.0 34.0 2.0 35.0 98-99 23.913326522301944 31.0 4.5 34.0 2.0 35.0 100-101 21.26689353014084 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08404556192704504 1101 2 -0.04335832819300478 1101 3 -0.08893671477071052 1101 4 -0.030846431534961027 1101 5 -0.12211617496677007 1101 6 -0.25266411703411507 1101 7 -0.10126683033278283 1101 8 -0.18544079025662796 1101 9 -0.13051566815214244 1101 10-11 -0.19142459329113137 1101 12-13 -0.12232909518994717 1101 14-15 -0.23869285157705633 1101 16-17 -0.17390805073374338 1101 18-19 -0.04608977422672211 1101 20-21 -0.034229887451196817 1101 22-23 -0.03962464621513817 1101 24-25 -0.0913297809886302 1101 26-27 -0.11791027859834458 1101 28-29 -0.15247601636122 1101 30-31 0.015380375843797367 1101 32-33 -0.19578645378985016 1101 34-35 -0.1398426091157816 1101 36-37 -0.03800084189891351 1101 38-39 -0.2874014085435235 1101 40-41 -0.3401110022815459 1101 42-43 -0.2518420175986975 1101 44-45 -0.12879835268268636 1101 46-47 -0.32012922948231193 1101 48-49 -0.32653693399979744 1101 50-51 -0.2570466532130524 1101 52-53 -0.25157459010706873 1101 54-55 -0.18326040896134543 1101 56-57 -0.062043715494674245 1101 58-59 -0.17458443164782267 1101 60-61 -0.05411545609560875 1101 62-63 -0.09208371137772531 1101 64-65 -0.2046537113658502 1101 66-67 -0.17370512770624202 1101 68-69 -0.023513772493998886 1101 70-71 -0.08733630878435505 1101 72-73 -0.054439704671597866 1101 74-75 -0.03064913773146216 1101 76-77 -0.07692194220559401 1101 78-79 -0.08736505840238351 1101 80-81 0.19810815427551276 1101 82-83 0.10145267528336888 1101 84-85 0.25582687992109854 1101 86-87 0.10465571160005283 1101 88-89 0.13883388781291828 1101 90-91 0.14485609618123263 1101 92-93 0.2528626172590869 1101 94-95 0.17424493562043253 1101 96-97 0.41704117104909955 1101 98-99 0.5310262441936366 1101 100-101 0.8549615406475901 1102 1 -0.07104247623968263 1102 2 -0.1591837193854282 1102 3 -0.014236638782339384 1102 4 -0.14502226018949216 1102 5 -0.10195305629384421 1102 6 -0.1632898076357705 1102 7 -0.11082494893968686 1102 8 -0.09609630249507006 1102 9 -0.19944752954628342 1102 10-11 -0.17988113471686518 1102 12-13 -0.007852778042810371 1102 14-15 -0.024334145285102693 1102 16-17 -0.10517791141131738 1102 18-19 -0.11154812497375843 1102 20-21 -0.1536222304193373 1102 22-23 -0.2060336397391609 1102 24-25 -0.07818979978762286 1102 26-27 -0.3880125824333973 1102 28-29 -0.2771779597520876 1102 30-31 -0.26449767354282017 1102 32-33 -0.3030327991127564 1102 34-35 -0.08068457616798952 1102 36-37 -0.17970623054087298 1102 38-39 -0.20960797677322063 1102 40-41 -0.3539859448216447 1102 42-43 -0.3264769830134142 1102 44-45 -0.2136245074510157 1102 46-47 -0.2232649319884743 1102 48-49 -0.4720755390316711 1102 50-51 -0.5116367356443163 1102 52-53 -0.5787241230363307 1102 54-55 -0.46543905561853904 1102 56-57 -0.3965711213658736 1102 58-59 -0.5539351776928712 1102 60-61 -0.23666832169219276 1102 62-63 -0.30674411322854667 1102 64-65 -0.4386856804445074 1102 66-67 -0.5798977609951095 1102 68-69 -0.5595351145613776 1102 70-71 -0.45396656465257834 1102 72-73 -0.4446925310882328 1102 74-75 -0.5746967603292745 1102 76-77 -0.5409204102255103 1102 78-79 -0.6390662620746461 1102 80-81 -0.5793993590018651 1102 82-83 -0.4777075815064826 1102 84-85 -0.45765570928429256 1102 86-87 -0.45321218053707213 1102 88-89 -0.6606260423448056 1102 90-91 -0.7584693287380411 1102 92-93 -0.5795175597593065 1102 94-95 -0.389991793076657 1102 96-97 -0.3932609831949385 1102 98-99 -0.27757607675335194 1102 100-101 0.0834424250494088 1103 1 -0.07087233789100011 1103 2 -0.17159147791598528 1103 3 -0.06303542321525057 1103 4 0.03664791878717466 1103 5 -0.11170612966908777 1103 6 -0.12918531911712705 1103 7 0.01854744716013812 1103 8 -0.026108845865095986 1103 9 -0.04051584862099844 1103 10-11 -0.05873486537801398 1103 12-13 0.010538595069341739 1103 14-15 -0.1641707431393371 1103 16-17 -0.1430879801904723 1103 18-19 -0.16680038038150968 1103 20-21 -0.1582235229161384 1103 22-23 -0.07053695639699953 1103 24-25 -0.13028097619377377 1103 26-27 -0.16294277301884819 1103 28-29 -0.18911971669468386 1103 30-31 -0.3263659811917776 1103 32-33 -0.2940141493428996 1103 34-35 -0.15865598626962907 1103 36-37 -0.11767333102566369 1103 38-39 -0.21054172709110475 1103 40-41 -0.11476705871616133 1103 42-43 -0.12453427207582735 1103 44-45 -0.0058895355758394885 1103 46-47 0.05196296604166406 1103 48-49 -0.10610725770076357 1103 50-51 -0.21347094351926899 1103 52-53 -0.15678933802215766 1103 54-55 -0.12311515158267383 1103 56-57 -0.06596339883198254 1103 58-59 -0.10474899919430669 1103 60-61 -0.15728699575564775 1103 62-63 -0.017429259110734563 1103 64-65 -0.19030192542597746 1103 66-67 -0.0846046446512645 1103 68-69 0.052772419031583695 1103 70-71 -0.04688220903320328 1103 72-73 -0.10820789442858825 1103 74-75 -0.10795547960765717 1103 76-77 -0.010441726605463941 1103 78-79 -0.0070203227792369205 1103 80-81 -0.058799792570393805 1103 82-83 0.031175595429910885 1103 84-85 0.07950780295915294 1103 86-87 0.19911662104278705 1103 88-89 0.3039313109181485 1103 90-91 0.18825233962169108 1103 92-93 0.37762976230191114 1103 94-95 0.06255777237031168 1103 96-97 0.10580006665989572 1103 98-99 0.19915504281244978 1103 100-101 0.6250056151531531 1104 1 0.11123087147023725 1104 2 0.0222860326619454 1104 3 0.038624546312153285 1104 4 0.065280100046472 1104 5 0.04048869565136215 1104 6 0.05873808833298 1104 7 0.04383058695869835 1104 8 0.04549694449453057 1104 9 5.505337404514421E-4 1104 10-11 0.18083837671493797 1104 12-13 0.07930647620469955 1104 14-15 0.13895176383476837 1104 16-17 0.0034365470794455177 1104 18-19 0.05979030107341288 1104 20-21 0.08538951813240914 1104 22-23 0.0011216316154119 1104 24-25 0.0882100074392369 1104 26-27 -0.014209520349389493 1104 28-29 0.09360053161564252 1104 30-31 -0.03801365297099579 1104 32-33 -0.016641185025051186 1104 34-35 0.13098386862599654 1104 36-37 0.04711762707196954 1104 38-39 0.09440534220036056 1104 40-41 0.16826204878552176 1104 42-43 0.001267388799611524 1104 44-45 -0.008516678834986635 1104 46-47 0.18051789003852292 1104 48-49 0.16817484992491671 1104 50-51 -0.023555521678765956 1104 52-53 -0.07323669862078219 1104 54-55 -0.09352751682126126 1104 56-57 -0.1382691473498241 1104 58-59 -0.07342286370505491 1104 60-61 9.00575478716803E-4 1104 62-63 0.1523042953404854 1104 64-65 0.20735484963111617 1104 66-67 0.23320098864596872 1104 68-69 0.19430862972847507 1104 70-71 0.20621176172963374 1104 72-73 0.28539988100954616 1104 74-75 0.25829603425533776 1104 76-77 0.15268292058448196 1104 78-79 0.21984326450561653 1104 80-81 0.3262810911688909 1104 82-83 0.5256980866638017 1104 84-85 0.7345839929446427 1104 86-87 0.7208900616435869 1104 88-89 0.6164277368143054 1104 90-91 0.7325018684061781 1104 92-93 0.7916136099004198 1104 94-95 0.843583966234231 1104 96-97 0.801805478348534 1104 98-99 1.1156264666844642 1104 100-101 1.3249129573957497 1105 1 0.041194384812936136 1105 2 0.09620893773272599 1105 3 -0.0753446310550423 1105 4 0.029023810039326747 1105 5 -0.07102966911011066 1105 6 0.10140148649202985 1105 7 0.0980524014065054 1105 8 0.07911679300885766 1105 9 0.048361689369038174 1105 10-11 -0.11080953059276055 1105 12-13 -0.042525866965881676 1105 14-15 -0.12421240623935859 1105 16-17 -0.2444533623501286 1105 18-19 -0.19356664183043648 1105 20-21 0.01814996313044759 1105 22-23 0.080640317357215 1105 24-25 -0.045011527215990554 1105 26-27 -0.021689498136410634 1105 28-29 -0.07581622156894952 1105 30-31 0.006536711824566055 1105 32-33 -0.06287115748607164 1105 34-35 0.04585335319693229 1105 36-37 0.00674504844953816 1105 38-39 0.06551398835932076 1105 40-41 0.11547292859827252 1105 42-43 0.13277473228973946 1105 44-45 0.1604511279746319 1105 46-47 0.14140104041780432 1105 48-49 0.16036061231709908 1105 50-51 -0.01043026691838378 1105 52-53 0.1326245299385178 1105 54-55 0.15584280783755133 1105 56-57 0.13940965360402657 1105 58-59 0.25765570120043435 1105 60-61 0.33815020017318176 1105 62-63 0.32653092449317 1105 64-65 0.26043661581825006 1105 66-67 0.14535293448266629 1105 68-69 0.029149623744892494 1105 70-71 -0.046781734868112324 1105 72-73 0.29457359811793893 1105 74-75 0.06233917912398468 1105 76-77 0.066021428423646 1105 78-79 0.27862534300945185 1105 80-81 0.4041745654684519 1105 82-83 0.42632399829763656 1105 84-85 0.520401349458627 1105 86-87 0.5093209933528939 1105 88-89 0.47417685518361097 1105 90-91 0.704612157813969 1105 92-93 0.7938845457000987 1105 94-95 0.8747322662457364 1105 96-97 0.9579121290324615 1105 98-99 0.9048112050641954 1105 100-101 1.1073918344759086 1106 1 -0.20947560880617289 1106 2 -0.10036252349145514 1106 3 -0.13318408020314365 1106 4 0.07873381280121095 1106 5 -0.07354774036657119 1106 6 -0.1884909907224781 1106 7 -0.4384129655899969 1106 8 -0.4505448292614034 1106 9 -0.5269566409325748 1106 10-11 -0.8865592853570021 1106 12-13 -0.5113537828904882 1106 14-15 -0.5199886369419247 1106 16-17 -0.31924217388525733 1106 18-19 -0.6064084242896541 1106 20-21 -0.5061564720463636 1106 22-23 -0.4958360971531235 1106 24-25 -0.5137480154399086 1106 26-27 -0.736776021121905 1106 28-29 -0.2839923341773911 1106 30-31 -0.3068431607480804 1106 32-33 -0.26207973645199445 1106 34-35 -0.05503044790972211 1106 36-37 -0.3976215766302218 1106 38-39 -0.36834473679567736 1106 40-41 -0.20415831300894638 1106 42-43 -0.2583005908024134 1106 44-45 -0.07608090505362952 1106 46-47 -0.018589816812131232 1106 48-49 0.21930671759228204 1106 50-51 0.11713708958298952 1106 52-53 0.20179625211259378 1106 54-55 -0.01515842072218021 1106 56-57 -0.09843474787786732 1106 58-59 -0.10824748067402368 1106 60-61 -0.0778286952659073 1106 62-63 -0.06259870284371516 1106 64-65 0.11507609544224806 1106 66-67 0.12963784605494055 1106 68-69 -0.013863299941554885 1106 70-71 0.0654114823393428 1106 72-73 -0.10933017422708602 1106 74-75 0.2087777844706018 1106 76-77 0.4116371844640625 1106 78-79 0.38602336779016966 1106 80-81 -0.1445773511799615 1106 82-83 0.01601076318558725 1106 84-85 -0.1422637680164449 1106 86-87 0.020583743231462392 1106 88-89 0.3591655695768097 1106 90-91 0.4987941179857778 1106 92-93 0.5276166053947655 1106 94-95 0.7296707906407427 1106 96-97 0.9848528248353574 1106 98-99 0.82585576654456 1106 100-101 1.0665084143180046 1107 1 -0.030709487054053852 1107 2 -0.12647679094221687 1107 3 -0.04443920930633638 1107 4 0.051425098179009865 1107 5 0.06483998477328612 1107 6 0.01120120772611699 1107 7 0.0493619982599256 1107 8 0.00755481724660001 1107 9 0.039841241215597734 1107 10-11 -0.02021734809684972 1107 12-13 0.08117136346287879 1107 14-15 0.14320922188124285 1107 16-17 0.06919836468625817 1107 18-19 0.14029348577766854 1107 20-21 0.08076092863228013 1107 22-23 0.20716670258617143 1107 24-25 0.20765430480804525 1107 26-27 0.17012840317477895 1107 28-29 0.16014138898869135 1107 30-31 0.09604043047669819 1107 32-33 0.11177816847497724 1107 34-35 0.17413142396658543 1107 36-37 0.021716480263606286 1107 38-39 0.1424289080101886 1107 40-41 0.28866068417802637 1107 42-43 0.37015702817173946 1107 44-45 0.2880461158628478 1107 46-47 0.3817139250065864 1107 48-49 0.29279924657692646 1107 50-51 0.288755424516836 1107 52-53 0.14975763888775617 1107 54-55 0.148729666472029 1107 56-57 0.16895600287003987 1107 58-59 0.10041576745167191 1107 60-61 0.22588308142812963 1107 62-63 0.2765410324174269 1107 64-65 0.4373111037720818 1107 66-67 0.18604318157708377 1107 68-69 0.20693382488194345 1107 70-71 0.34224886199594806 1107 72-73 0.45723918187715995 1107 74-75 0.4471893513133587 1107 76-77 0.23790756709017913 1107 78-79 0.3643565005973137 1107 80-81 0.46159395920579627 1107 82-83 0.6719462031019141 1107 84-85 0.7180903587756511 1107 86-87 0.7826555798323902 1107 88-89 0.8760349399270311 1107 90-91 0.8149218858930745 1107 92-93 0.8316835717927269 1107 94-95 0.9565046641645694 1107 96-97 1.2174144644949614 1107 98-99 1.3700282417045315 1107 100-101 1.5495684784322563 1108 1 0.1047142434547439 1108 2 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-0.48038405493893777 2316 12-13 -0.03126001708486825 2316 14-15 -0.20014451145612355 2316 16-17 -0.12067381892743612 2316 18-19 -0.13059020101801622 2316 20-21 -0.12136550584974515 2316 22-23 -0.22859699959003166 2316 24-25 -0.08736517945668965 2316 26-27 0.13455412728384175 2316 28-29 0.050940014501414055 2316 30-31 0.015849579346628673 2316 32-33 -0.05678555868480828 2316 34-35 -0.08426740180514969 2316 36-37 -0.17760213162525673 2316 38-39 -0.07041030996472131 2316 40-41 -0.1137626255022326 2316 42-43 0.13390673173571344 2316 44-45 0.018577221883994355 2316 46-47 0.010706101047141203 2316 48-49 0.04276992014791148 2316 50-51 0.05585780742338642 2316 52-53 0.0038385017581532566 2316 54-55 -0.1449001061481212 2316 56-57 -0.11605580089564427 2316 58-59 0.081070083511527 2316 60-61 0.1198984404743797 2316 62-63 0.0382362268843508 2316 64-65 0.28837773673694045 2316 66-67 0.06521643977750102 2316 68-69 0.17881783080382974 2316 70-71 0.20194712671064252 2316 72-73 0.11115443093710908 2316 74-75 0.22713261126068218 2316 76-77 0.14545026607805056 2316 78-79 0.387982281700161 2316 80-81 0.39773387896556045 2316 82-83 0.30232535820798034 2316 84-85 0.46227147802052215 2316 86-87 0.3438110899317124 2316 88-89 0.49466868942565156 2316 90-91 0.39423528636327276 2316 92-93 0.4854770258995522 2316 94-95 0.5417342935194931 2316 96-97 0.5768929517782198 2316 98-99 0.6898219710211038 2316 100-101 0.637119726488951 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9611.0 3 2276.0 4 1690.0 5 1860.0 6 2243.0 7 2622.0 8 3361.0 9 4261.0 10 5294.0 11 6830.0 12 8781.0 13 10838.0 14 12776.0 15 15071.0 16 18280.0 17 22225.0 18 19644.0 19 16195.0 20 16684.0 21 17636.0 22 19533.0 23 22087.0 24 24984.0 25 28478.0 26 33102.0 27 38708.0 28 46292.0 29 55434.0 30 67547.0 31 83789.0 32 106520.0 33 137855.0 34 184229.0 35 253782.0 36 361175.0 37 459398.0 38 317392.0 39 32017.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6806283827183 19.00156986126062 15.151615975174362 23.166185780846718 2 14.987636052103817 19.655310331847065 40.956307560505465 24.400746055543657 3 19.096220732733705 23.754403399008282 29.58560897208392 27.563766896174087 4 12.598846795832564 16.47268796355826 37.51815698260459 33.41030825800459 5 13.836441761141808 37.10537556073593 34.481461996108365 14.5767206820139 6 32.29279685396432 36.33258161085012 17.32599950822905 14.048622026956515 7 28.766141126519162 32.32838793237769 20.491196634465567 18.414274306637584 8 26.327371514721705 35.22076472048802 19.546972650069215 18.904891114721057 9 25.839056323308906 17.096837534159434 20.06915459862259 36.994951543909075 10-11 24.824989050087204 26.68506427837184 27.46889342280986 21.021053248731096 12-13 25.72686720619585 23.710875275600518 27.923150768192066 22.639106750011564 14-15 22.4536996655901 25.384218432991645 25.937578982684766 26.224502918733485 16-17 22.228612570240575 28.62470175840062 26.708145097010554 22.438540574348252 18-19 22.206059489764332 27.228391377850514 28.844101148097195 21.72144798428796 20-21 22.66160900496909 26.346986802237204 29.18700272084945 21.804401471944256 22-23 22.33254294563769 26.24735551422191 29.1006618567282 22.319439683412202 24-25 22.27555794616425 26.444323832286358 29.04916108294574 22.230957138603653 26-27 22.005341974826838 26.749022111913725 28.84830706294427 22.39732885031517 28-29 22.094653931566317 27.001466485362634 28.81074711231809 22.09313247075296 30-31 22.230722089044118 26.635925143559504 28.724356621954122 22.408996145442256 32-33 21.9766151104735 26.66055111382312 28.720330710488756 22.642503065214623 34-35 22.48325204413211 26.84986272366872 28.55301029050078 22.113874941698388 36-37 22.53102746611258 26.47143655727982 28.578797228805303 22.418738747802298 38-39 22.129258686740247 26.825504971573764 28.569739250230576 22.475497091455416 40-41 22.565706849608468 26.6182309532077 28.573839279050066 22.242222918133763 42-43 22.332200488228857 26.590481058566162 28.430537934084033 22.646780519120945 44-45 22.31167989665133 26.898040769410386 28.448534517753217 22.34174481618507 46-47 22.656081994019242 27.01386043001416 27.902798459771887 22.427259116194712 48-49 22.46774854874302 27.172046760774947 27.727453198953217 22.632751491528815 50-51 22.537884167327384 27.32896433576819 27.712289702599247 22.420861794305182 52-53 22.81752147409564 27.12120803214677 27.63166358471044 22.429606909047152 54-55 22.5096110168049 27.22456313974963 27.747348893148054 22.518476950297416 56-57 22.522901503455 27.41136138495377 27.633297662346934 22.432439449244296 58-59 22.51520105259038 27.0816919562943 27.73422741865716 22.668879572458163 60-61 22.293977111576048 27.293957846250127 27.872424606160973 22.539640436012856 62-63 22.34537848648401 27.255938767716724 28.02566203928197 22.373020706517295 64-65 22.20692290034044 27.278458834188967 27.907855811980305 22.606762453490287 66-67 22.206066446474935 27.323384755342406 27.732335433651777 22.73821336453088 68-69 22.48424949817013 27.079344078245306 27.927658623463124 22.508747800121437 70-71 22.421272298070136 27.152742287443317 27.643887956000096 22.78209745848645 72-73 22.362277638675547 27.177069318609092 27.681994862524046 22.77865818019131 74-75 22.308886565550754 27.50554947679259 27.512485688992005 22.673078268664646 76-77 22.451719948317372 27.24357973181017 27.59290398349726 22.711796336375198 78-79 22.416418290366515 27.32975617374408 27.589534627831057 22.664290908058344 80-81 22.535613465894652 27.358771634322377 27.354674810557395 22.750940089225576 82-83 22.524370057389746 27.35702960506169 27.50698358861745 22.611616748931116 84-85 22.477354011582335 27.46228034684132 27.37712149282518 22.68324414875116 86-87 22.586386323814168 27.470008248758376 27.303025146442334 22.64058028098512 88-89 22.520001752333606 27.474623613344345 27.18579279304129 22.819581841280762 90-91 22.428557403969727 27.48316873937144 27.311144249866942 22.777129606791892 92-93 22.575514629558796 27.20686629751874 27.44760612935375 22.77001294356872 94-95 22.435473746867867 27.042830241677475 27.5119074298103 23.009788581644354 96-97 22.62203064411378 26.879660741240148 27.542483405601065 22.95582520904501 98-99 22.41613204998284 27.033524462009424 27.35973056455035 23.190612923457387 100-101 22.729335695478017 26.540119145539588 27.800727300454746 22.929817858527652 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3334.0 1 1943.5 2 491.0 3 409.0 4 352.0 5 335.0 6 470.5 7 873.0 8 1411.5 9 1682.0 10 1523.5 11 1396.0 12 1517.5 13 1648.5 14 1766.5 15 1907.0 16 2094.0 17 2315.5 18 2550.5 19 2895.0 20 3322.0 21 3854.5 22 4709.5 23 5939.5 24 7474.5 25 9483.0 26 12382.5 27 16036.0 28 19432.0 29 23000.5 30 27153.0 31 32833.5 32 40746.5 33 45858.0 34 48617.5 35 52495.5 36 58222.5 37 64308.0 38 68757.0 39 72491.0 40 76534.5 41 81412.0 42 84010.5 43 85388.5 44 88189.0 45 91976.5 46 93877.5 47 94880.0 48 95634.5 49 94199.0 50 91490.0 51 88478.0 52 86817.0 53 86140.0 54 84837.5 55 78663.5 56 70887.5 57 64490.5 58 58709.0 59 51397.0 60 41263.0 61 33583.0 62 27654.5 63 20423.0 64 14160.0 65 10438.0 66 7612.5 67 5636.0 68 4212.5 69 3140.5 70 2436.0 71 1685.5 72 1143.5 73 840.5 74 583.5 75 410.5 76 296.5 77 194.5 78 118.0 79 77.0 80 50.0 81 35.0 82 25.0 83 17.5 84 15.5 85 18.0 86 12.5 87 7.5 88 7.0 89 4.5 90 3.0 91 4.5 92 4.0 93 2.5 94 3.5 95 2.0 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24096317266693165 2 0.2133169695875575 3 0.2055047921434591 4 0.2103621045439348 5 0.21109070140400615 6 0.2404369638235468 7 0.24246084399041165 8 0.23096520464261922 9 0.22626980265549268 10-11 0.23762377039160462 12-13 0.23817021803665814 14-15 0.22517690736538568 16-17 0.23669278551484677 18-19 0.22833416042569488 20-21 0.22112914703165593 22-23 0.2212910574450051 24-25 0.21453129768767643 26-27 0.22246490794178672 28-29 0.2333736220411884 30-31 0.23304980121448998 32-33 0.23266526398278567 34-35 0.24074054584857651 36-37 0.2197124309148505 38-39 0.22009696814655483 40-41 0.2149360737210494 42-43 0.22139225145334837 44-45 0.23620705427479918 46-47 0.24011314299684844 48-49 0.2428858588254533 50-51 0.24351326167718143 52-53 0.26021027305381655 54-55 0.24337159006550085 56-57 0.21776950595466024 58-59 0.20099153937135042 60-61 0.19965577846121962 62-63 0.20524168772176665 64-65 0.19785452511270987 66-67 0.19929148003118397 68-69 0.20339995676991965 70-71 0.2040880760266537 72-73 0.20599052338350665 74-75 0.2096537464855321 76-77 0.22088628141163213 78-79 0.21552299896944022 80-81 0.2095727912788575 82-83 0.2055857473501337 84-85 0.20682031425192127 86-87 0.2138229396292737 88-89 0.21121213421401805 90-91 0.22044102777492186 92-93 0.20987637330388723 94-95 0.2076298663186672 96-97 0.21708138669792615 98-99 0.22246490794178672 100-101 0.20020222610627314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2470502.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.036097979167366 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1380475704795 42.10442995622727 2 13.587405581837924 15.227703809331109 3 4.800515655609152 8.07006496884724 4 2.233493738902801 5.006250959560568 5 1.2031037295881775 3.370861923515239 6 0.7115988087879552 2.3925132342660382 7 0.476623501845065 1.8695684873994745 8 0.3385754985305793 1.5177959847203981 9 0.23757127984459064 1.1981310762966906 >10 1.1789214881030725 11.57572190335253 >50 0.06260188241317763 2.386353021821877 >100 0.028003225525559724 2.7893124159809135 >500 0.0022383509083708755 0.9133214258726503 >1k 0.0012274827562005872 1.31489477242549 >5k 7.220486801179924E-5 0.2630760603825016 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6502 0.263185376899108 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4843 0.1960330329625315 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3525 0.14268355176397346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3380 0.13681429928006533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.095520667459488E-5 0.0 0.0 0.0 0.0 3 8.095520667459488E-5 0.0 0.0 0.0 0.0 4 8.095520667459488E-5 0.0 0.0 0.0 0.0 5 1.6191041334918976E-4 0.0 0.0 0.0 0.0 6 1.6191041334918976E-4 0.0 0.0 0.0 0.0 7 1.6191041334918976E-4 0.0 0.0 0.0 0.0 8 1.6191041334918976E-4 0.0 0.0 0.0 0.0 9 1.6191041334918976E-4 0.0 0.0 0.0 0.0 10-11 1.6191041334918976E-4 0.0 0.0 0.0 0.0 12-13 1.6191041334918976E-4 0.0 0.0 0.0 1.2143281001189232E-4 14-15 1.6191041334918976E-4 0.0 0.0 4.047760333729744E-5 1.2143281001189232E-4 16-17 1.821492150178385E-4 0.0 0.0 4.047760333729744E-5 1.2143281001189232E-4 18-19 2.023880166864872E-4 0.0 0.0 6.071640500594616E-5 1.2143281001189232E-4 20-21 2.023880166864872E-4 0.0 0.0 8.095520667459488E-5 2.226268183551359E-4 22-23 2.023880166864872E-4 0.0 0.0 8.095520667459488E-5 2.4286562002378463E-4 24-25 2.833432233610821E-4 0.0 0.0 1.6191041334918976E-4 2.4286562002378463E-4 26-27 2.833432233610821E-4 0.0 0.0 3.64298430035677E-4 2.4286562002378463E-4 28-29 2.833432233610821E-4 0.0 0.0 0.0010321788851010848 2.4286562002378463E-4 30-31 2.833432233610821E-4 0.0 0.0 0.0028131934319421724 2.4286562002378463E-4 32-33 2.833432233610821E-4 0.0 0.0 0.00601092409558867 2.4286562002378463E-4 34-35 2.833432233610821E-4 0.0 0.0 0.009795580007625981 2.4286562002378463E-4 36-37 2.833432233610821E-4 0.0 0.0 0.015563638483190867 2.4286562002378463E-4 38-39 2.833432233610821E-4 0.0 0.0 0.02108883133873197 2.4286562002378463E-4 40-41 2.833432233610821E-4 0.0 0.0 0.026553307789267122 2.4286562002378463E-4 42-43 2.833432233610821E-4 0.0 0.0 0.03361664957162552 2.4286562002378463E-4 44-45 2.833432233610821E-4 0.0 0.0 0.04385748321596178 2.4286562002378463E-4 46-47 2.833432233610821E-4 0.0 0.0 0.05632458504384939 2.4286562002378463E-4 48-49 2.833432233610821E-4 0.0 0.0 0.07383114848723053 2.833432233610821E-4 50-51 2.833432233610821E-4 0.0 0.0 0.09121627912059978 2.833432233610821E-4 52-53 2.833432233610821E-4 0.0 0.0 0.11685884083477771 2.833432233610821E-4 54-55 2.833432233610821E-4 0.0 0.0 0.15367322107004971 2.833432233610821E-4 56-57 2.833432233610821E-4 0.0 0.0 0.20722509028529423 2.833432233610821E-4 58-59 2.833432233610821E-4 0.0 0.0 0.2608376759055447 2.833432233610821E-4 60-61 2.833432233610821E-4 0.0 0.0 0.31776942499945354 2.833432233610821E-4 62-63 2.833432233610821E-4 0.0 0.0 0.3825943067441354 2.833432233610821E-4 64-65 2.833432233610821E-4 0.0 0.0 0.45506945551956646 2.833432233610821E-4 66-67 2.833432233610821E-4 0.0 0.0 0.5547455537376613 2.833432233610821E-4 68-69 3.238208266983795E-4 0.0 0.0 0.6776760350730338 2.833432233610821E-4 70-71 3.238208266983795E-4 0.0 0.0 0.8045733215354612 2.833432233610821E-4 72-73 3.238208266983795E-4 0.0 0.0 0.9449901275125461 2.833432233610821E-4 74-75 3.238208266983795E-4 0.0 0.0 1.093300066140404 2.833432233610821E-4 76-77 3.238208266983795E-4 0.0 0.0 1.2606547171384601 2.833432233610821E-4 78-79 3.238208266983795E-4 0.0 0.0 1.4413669772378246 2.833432233610821E-4 80-81 4.047760333729744E-4 0.0 0.0 1.6414275317324172 2.833432233610821E-4 82-83 4.4525363671027186E-4 0.0 0.0 1.8569302919001887 2.833432233610821E-4 84-85 4.4525363671027186E-4 0.0 0.0 2.102973403785951 2.833432233610821E-4 86-87 4.4525363671027186E-4 0.0 0.0 2.3689112577119955 3.238208266983795E-4 88-89 4.4525363671027186E-4 0.0 0.0 2.6629203295524553 3.64298430035677E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4165 0.0 49.960773 1 GTATCAA 7465 0.0 42.448864 1 TCAACGC 9500 0.0 32.90646 4 ATCAACG 9500 0.0 32.901787 3 TATCAAC 9690 0.0 32.403717 2 CAACGCA 9720 0.0 32.162968 5 AACGCAG 10120 0.0 31.075712 6 ACGCAGA 11335 0.0 27.534033 7 CGCAGAG 11465 0.0 27.179293 8 GCAGAGT 12900 0.0 23.52987 9 TAAGGTG 1335 0.0 23.48871 5 TACATGG 7355 0.0 22.475725 2 GTACATG 7805 0.0 22.399843 1 GAGTACT 7500 0.0 22.230078 12-13 AGAGTAC 10980 0.0 22.150116 10-11 AGTACTT 8085 0.0 20.856598 12-13 GTACTTT 8055 0.0 20.816126 14-15 CAGAGTA 11865 0.0 20.698132 10-11 ACATGGG 7625 0.0 20.1847 3 GTAAGGT 1485 0.0 18.555832 4 >>END_MODULE