FastQCFastQC Report
Thu 26 May 2016
SRR937216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470502
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75140.30414871147645295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59080.23914168051675327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.17352748550699412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.046.8876461
GTATCAA76250.040.019891
ATCAACG98700.030.5108183
TATCAAC99350.030.4546282
TCAACGC99450.030.2807224
CAACGCA101550.029.607765
AACGCAG105400.028.5701716
ACGCAGA120550.024.8265657
CGCAGAG122150.024.2291348
TACATGG76800.021.955782
GTACATG79250.021.7115121
ACATGGG74400.021.3233433
GCAGAGT137650.021.0866859
GAGTACT84350.020.78186812-13
TAAGGTG14250.020.6661035
AGAGTAC119250.020.49605410-11
GTACTTT86900.019.7857714-15
GTAAGGT14500.019.6546364
AGTACTT89100.019.48735812-13
AGGTAAG14800.019.2562332
Produced by FastQC (version 0.11.5)