##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937216_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2470502 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01880872794274 31.0 31.0 34.0 30.0 34.0 2 32.23986703916856 33.0 31.0 34.0 30.0 34.0 3 32.30273887655221 34.0 31.0 34.0 30.0 34.0 4 35.987434537596 37.0 35.0 37.0 35.0 37.0 5 35.893792030931365 37.0 35.0 37.0 35.0 37.0 6 35.93309821242808 37.0 35.0 37.0 35.0 37.0 7 35.871545540137184 37.0 35.0 37.0 35.0 37.0 8 35.83627578524526 37.0 35.0 37.0 35.0 37.0 9 37.52857718795613 39.0 37.0 39.0 35.0 39.0 10-11 37.42903588015715 39.0 37.0 39.0 34.0 39.0 12-13 37.32646968106077 39.0 37.0 39.0 34.0 39.0 14-15 38.4970635522659 40.0 38.0 41.0 34.0 41.0 16-17 38.45093284684651 40.0 38.0 41.0 34.0 41.0 18-19 38.41510126282027 40.0 38.0 41.0 34.0 41.0 20-21 38.2976409247999 40.0 38.0 41.0 34.0 41.0 22-23 38.14043441373454 40.0 37.5 41.0 33.0 41.0 24-25 38.07926486195923 40.0 37.0 41.0 33.0 41.0 26-27 38.02683057937213 40.0 37.0 41.0 33.0 41.0 28-29 37.9002753286579 40.0 37.0 41.0 33.0 41.0 30-31 37.98951488401953 40.0 37.0 41.0 33.0 41.0 32-33 37.94640684362935 40.0 37.0 41.0 33.0 41.0 34-35 37.82790400493503 40.0 37.0 41.0 33.0 41.0 36-37 37.65650442703548 40.0 37.0 41.0 33.0 41.0 38-39 37.52358063260017 40.0 37.0 41.0 32.5 41.0 40-41 37.34038486914805 40.0 36.5 41.0 32.0 41.0 42-43 37.139889585193615 39.5 36.0 41.0 31.0 41.0 44-45 36.87786712983839 39.0 36.0 41.0 31.0 41.0 46-47 36.58916791000372 39.0 36.0 41.0 30.5 41.0 48-49 36.29883116872603 39.0 35.0 40.0 30.0 41.0 50-51 36.0613865117292 39.0 35.0 40.0 29.5 41.0 52-53 35.803111877666964 38.5 35.0 40.0 28.5 41.0 54-55 35.55532053809307 38.0 35.0 40.0 28.0 41.0 56-57 35.261379266238194 38.0 34.0 40.0 28.0 41.0 58-59 34.96531332498415 38.0 34.0 40.0 27.0 41.0 60-61 34.61728162940163 37.5 34.0 40.0 26.0 41.0 62-63 34.22471728417949 37.0 33.0 40.0 26.0 41.0 64-65 33.79425436611668 36.0 33.0 39.0 25.5 40.5 66-67 33.35211871919148 36.0 32.0 39.0 24.5 40.0 68-69 32.89452083017945 35.0 32.0 38.5 23.0 40.0 70-71 32.46744406602383 35.0 31.0 38.0 22.5 40.0 72-73 31.969650500181743 35.0 31.0 37.0 21.5 39.0 74-75 31.475839323343997 34.5 31.0 36.5 20.0 39.0 76-77 30.26013599665169 33.5 29.5 35.5 20.0 37.5 78-79 30.886913469408242 34.0 30.0 36.0 20.0 38.0 80-81 30.705096373125784 34.0 30.0 35.5 19.0 37.0 82-83 30.33166882682143 34.0 30.0 35.0 18.0 37.0 84-85 29.93913241114559 34.0 30.0 35.0 15.0 36.0 86-87 29.486014988046964 34.0 29.0 35.0 8.5 36.0 88-89 28.95521031757918 33.5 29.0 35.0 4.5 35.5 90-91 28.376986944353817 33.0 28.5 35.0 2.0 35.0 92-93 27.938219843578352 33.0 27.0 35.0 2.0 35.0 94-95 27.400916898670793 33.0 26.5 34.5 2.0 35.0 96-97 26.81384653807202 32.5 25.5 34.0 2.0 35.0 98-99 25.897544911924783 32.0 24.5 34.0 2.0 35.0 100-101 23.2282420738781 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030297142040645042 1101 2 0.07858214108573947 1101 3 0.09079770054852077 1101 4 0.10068720415964805 1101 5 0.011349772015257997 1101 6 0.07819147254357972 1101 7 0.09186572026901274 1101 8 0.043201252281562574 1101 9 0.13278443236387005 1101 10-11 0.13773819500927686 1101 12-13 0.07117525622119558 1101 14-15 0.016528243941934306 1101 16-17 0.037066563915317374 1101 18-19 0.1472939256248651 1101 20-21 0.13104863064832983 1101 22-23 0.12371604774001099 1101 24-25 0.1389792365717284 1101 26-27 0.012585226894820778 1101 28-29 0.007556194182974707 1101 30-31 -0.041619108894551005 1101 32-33 0.11665248424376529 1101 34-35 0.09934965006208785 1101 36-37 0.13752257632913967 1101 38-39 0.015519119284995497 1101 40-41 0.1734351474600686 1101 42-43 0.22321923233381114 1101 44-45 0.15624172585954454 1101 46-47 0.1032865089241497 1101 48-49 0.03695541014332093 1101 50-51 0.1194424719299434 1101 52-53 0.2558249761567879 1101 54-55 0.25145076572996317 1101 56-57 0.33275411860438453 1101 58-59 0.236591802344428 1101 60-61 0.15828946201548177 1101 62-63 0.08682910986309622 1101 64-65 0.28022529769888394 1101 66-67 0.42642533069941635 1101 68-69 0.3208541894532928 1101 70-71 0.3313971254914563 1101 72-73 0.3385290975687276 1101 74-75 0.05272372291838323 1101 76-77 0.2534770876500154 1101 78-79 0.14606786305016328 1101 80-81 0.15504801106073884 1101 82-83 0.1288940734600068 1101 84-85 0.184329371592856 1101 86-87 0.20456336456944868 1101 88-89 0.22955267713742344 1101 90-91 0.23685931516639513 1101 92-93 0.28323940030654526 1101 94-95 0.2489057628836875 1101 96-97 0.2879293113180097 1101 98-99 0.5218589159825413 1101 100-101 0.9763226296796113 1102 1 -0.0979515170152574 1102 2 -0.05695208584583611 1102 3 -0.08927727104637029 1102 4 -0.09456996272521678 1102 5 -0.0763166096417649 1102 6 -0.0797574066385991 1102 7 -0.10867967486554875 1102 8 -0.11165881536810929 1102 9 -0.2229767599449275 1102 10-11 -0.10664422516423855 1102 12-13 -0.2042962913101647 1102 14-15 -0.13812413730866524 1102 16-17 -0.234010268407701 1102 18-19 -0.26986085733254583 1102 20-21 -0.10069012556504475 1102 22-23 -0.2243246068193443 1102 24-25 -0.2535751101661461 1102 26-27 -0.2040034277900844 1102 28-29 -0.24946562971685893 1102 30-31 -0.3088381574792507 1102 32-33 -0.10491020644607829 1102 34-35 -0.1789305986276446 1102 36-37 -0.2522933631085138 1102 38-39 -0.12431098543013519 1102 40-41 -0.13444584489607792 1102 42-43 -0.19414692922533305 1102 44-45 -0.10139215163345483 1102 46-47 0.009367524086741241 1102 48-49 -0.01329887611343139 1102 50-51 0.041312268675604 1102 52-53 -0.009282913554848449 1102 54-55 -0.12142740706143229 1102 56-57 -0.009332859181483855 1102 58-59 -0.0689579190575742 1102 60-61 -0.1641005376820459 1102 62-63 -0.1795324246560881 1102 64-65 -0.13416693663328516 1102 66-67 -0.09197149502876556 1102 68-69 -0.17306468177786627 1102 70-71 -0.0954137614508781 1102 72-73 -0.054797061530837965 1102 74-75 -0.23424860984507134 1102 76-77 -0.10542141185302967 1102 78-79 0.008787792296200081 1102 80-81 -0.09090288520015477 1102 82-83 -0.08164852838123693 1102 84-85 -0.1434570662935748 1102 86-87 -0.09402698086024941 1102 88-89 0.05709550065283864 1102 90-91 -0.12322743455494845 1102 92-93 -0.05134807486161819 1102 94-95 0.11956044863676851 1102 96-97 0.19744357791804745 1102 98-99 0.3843215981093131 1102 100-101 0.9441787394300505 1103 1 0.01689131369861485 1103 2 0.09534669539784346 1103 3 0.0034156820644994923 1103 4 -0.0479940697900787 1103 5 0.027906787140551614 1103 6 0.04575921353468004 1103 7 0.028773807479581137 1103 8 -0.03848296331054968 1103 9 0.019702645882588854 1103 10-11 -0.05919593987314897 1103 12-13 -0.005152543402232368 1103 14-15 0.015903621171986515 1103 16-17 -0.09750204533150963 1103 18-19 -0.0698256510352877 1103 20-21 -0.14250055012331586 1103 22-23 -0.023941566742408327 1103 24-25 -0.10843253922178775 1103 26-27 -0.009915229290349714 1103 28-29 -0.062243144498921765 1103 30-31 -0.12896954532840965 1103 32-33 -0.019959934820967362 1103 34-35 0.025445146160954835 1103 36-37 -0.06901851743245402 1103 38-39 -0.057125611726171144 1103 40-41 0.09350799672245813 1103 42-43 0.11152304564078719 1103 44-45 0.12624429252775116 1103 46-47 0.08507118551392523 1103 48-49 0.08719042070075034 1103 50-51 0.2050268200663936 1103 52-53 0.2379515409721762 1103 54-55 0.016537460964180184 1103 56-57 0.015224677115398322 1103 58-59 0.07330207563443736 1103 60-61 0.04661934304639459 1103 62-63 0.15276440977226713 1103 64-65 0.06720587323412985 1103 66-67 0.33599890283984024 1103 68-69 0.14648668267067677 1103 70-71 0.22714878831146024 1103 72-73 0.2116529733981345 1103 74-75 0.3084561156747654 1103 76-77 0.4591383957284023 1103 78-79 0.43930770014694076 1103 80-81 0.40520341479965083 1103 82-83 0.3931245442230491 1103 84-85 0.3951666267818368 1103 86-87 0.3001857916349664 1103 88-89 0.5638186000050354 1103 90-91 0.6205902481302346 1103 92-93 0.5396726137951866 1103 94-95 0.659336562100048 1103 96-97 0.7465227370380312 1103 98-99 0.9225103082997812 1103 100-101 1.5024284526075782 1104 1 0.06421046687521681 1104 2 0.06119921187835331 1104 3 0.026780271230819608 1104 4 0.017892871326246507 1104 5 0.039391762881940906 1104 6 0.011818957263649565 1104 7 0.05482102698250202 1104 8 0.043476828941308554 1104 9 0.0860918016693546 1104 10-11 0.0559749914661225 1104 12-13 0.017769847728445143 1104 14-15 -0.03932937642679235 1104 16-17 -0.047594108584512185 1104 18-19 0.10925209711791695 1104 20-21 0.010867996743733954 1104 22-23 -0.03696928140521294 1104 24-25 0.027159476753958245 1104 26-27 -0.13363428462852056 1104 28-29 -0.03592004664406545 1104 30-31 -0.13295399875521952 1104 32-33 0.011670388753621808 1104 34-35 0.09675878007193717 1104 36-37 -0.08389768865934144 1104 38-39 -0.12427884721729043 1104 40-41 -0.09013370510018603 1104 42-43 0.0012647023377070354 1104 44-45 0.07984393174851334 1104 46-47 -0.0659091522438473 1104 48-49 0.08827043702980575 1104 50-51 0.014792489079184179 1104 52-53 0.1282244298162709 1104 54-55 0.18864719288799137 1104 56-57 0.0457584231668946 1104 58-59 -0.011694360170132256 1104 60-61 0.20317018704447776 1104 62-63 0.1198767093888975 1104 64-65 0.246150990286381 1104 66-67 0.2651789906792814 1104 68-69 0.3163030430920841 1104 70-71 0.2777517290133318 1104 72-73 0.36711332646580885 1104 74-75 0.3386553822261895 1104 76-77 0.37685997742717703 1104 78-79 0.2148909231195013 1104 80-81 0.19418011419048398 1104 82-83 0.24585164915080782 1104 84-85 0.30363319043881987 1104 86-87 0.3086303020096359 1104 88-89 0.39742177228052 1104 90-91 0.3144970592822318 1104 92-93 0.6365598453693728 1104 94-95 0.8332469346165077 1104 96-97 0.9032588682411884 1104 98-99 1.1854260579670033 1104 100-101 1.6649154230527792 1105 1 8.635794611961956E-4 1105 2 0.03832325657131719 1105 3 0.01532425618734834 1105 4 0.036605598196132405 1105 5 -0.06580698217020142 1105 6 -0.05787623925158414 1105 7 -0.09839140376173816 1105 8 0.013743114787068578 1105 9 -0.06551544339627924 1105 10-11 -0.02849666213645463 1105 12-13 0.004967632680148881 1105 14-15 0.02009182219327954 1105 16-17 -0.08016842760202536 1105 18-19 -0.04576243931406054 1105 20-21 -0.0695315385449291 1105 22-23 -0.08917694431337253 1105 24-25 -0.07361655709383541 1105 26-27 -0.0029064546401471603 1105 28-29 -0.08603054341082839 1105 30-31 -0.0832406796340237 1105 32-33 -0.023541010876819257 1105 34-35 0.014266623496027364 1105 36-37 -0.03922603306839534 1105 38-39 -0.15041559335736565 1105 40-41 -0.11680077891004004 1105 42-43 -0.05083885500486218 1105 44-45 -0.07038806885368842 1105 46-47 -0.0449711511559201 1105 48-49 -0.05320966574070951 1105 50-51 -0.05581267245486998 1105 52-53 -0.13679155528797082 1105 54-55 -0.007056286623949859 1105 56-57 -0.09174024533286484 1105 58-59 -0.06656125985525563 1105 60-61 -0.09229404850395184 1105 62-63 -0.05777737892046275 1105 64-65 -0.07700781117876687 1105 66-67 0.01054210322264737 1105 68-69 0.228784916935453 1105 70-71 0.23005376865865657 1105 72-73 0.2587863724980828 1105 74-75 0.3343892554503256 1105 76-77 0.2955371266105935 1105 78-79 0.27344604296872177 1105 80-81 0.16148024357680058 1105 82-83 0.17565438827760005 1105 84-85 0.2508891637264412 1105 86-87 0.11430156986771678 1105 88-89 0.48905213929169733 1105 90-91 0.48840469648357043 1105 92-93 0.5195950410040417 1105 94-95 0.7879275662944245 1105 96-97 0.6740012985735468 1105 98-99 0.8878493173943802 1105 100-101 1.574357570609994 1106 1 -0.21950384561094438 1106 2 -0.1197742853568542 1106 3 -0.0913299661533884 1106 4 -0.10180260744000691 1106 5 -0.12999303754110514 1106 6 -0.13087744876387575 1106 7 -0.1716326109883326 1106 8 -0.16023999013574297 1106 9 -0.13201766718462693 1106 10-11 -0.25849161456547876 1106 12-13 -0.25611178561111103 1106 14-15 -0.24238282090535535 1106 16-17 -0.1907845169726059 1106 18-19 -0.11540177608227964 1106 20-21 -0.11717394195061814 1106 22-23 -0.20827961952598883 1106 24-25 -0.1672785222179769 1106 26-27 -0.11778726529909278 1106 28-29 -0.20437322586146678 1106 30-31 -0.1882120608589588 1106 32-33 -0.22840382170719664 1106 34-35 -0.09392472706253585 1106 36-37 -0.18469131435143282 1106 38-39 -0.2176016201571045 1106 40-41 -0.11849409611654238 1106 42-43 -0.19150418200174357 1106 44-45 -0.14857684800816884 1106 46-47 -0.18043271818861228 1106 48-49 -0.2635095676464019 1106 50-51 -0.09030091542398822 1106 52-53 -0.02136598791241795 1106 54-55 0.024861504635182996 1106 56-57 -0.035296020992149124 1106 58-59 -0.10794848473882013 1106 60-61 -0.0469887656971224 1106 62-63 0.09000216603624978 1106 64-65 0.06947365090254465 1106 66-67 0.1947698724819915 1106 68-69 0.16721217000922906 1106 70-71 0.221045283025191 1106 72-73 0.1521921495859182 1106 74-75 0.13168642404871989 1106 76-77 0.33072917240063404 1106 78-79 0.2340749893597085 1106 80-81 0.25829836911846016 1106 82-83 0.14356707482344433 1106 84-85 0.16836994962371676 1106 86-87 0.3382089612258916 1106 88-89 0.5714742114624407 1106 90-91 0.4436403609058104 1106 92-93 0.7604517947492404 1106 94-95 0.7812523917722807 1106 96-97 0.8781784016487535 1106 98-99 1.0122328946936427 1106 100-101 1.8091486678376363 1107 1 -0.02548228178714851 1107 2 -0.08129923905745073 1107 3 -0.028033418919136466 1107 4 -0.019164607899121222 1107 5 -0.005895719105268427 1107 6 -0.10078420039459957 1107 7 -0.12657016860885761 1107 8 -0.047124322446272515 1107 9 -0.14446790777914487 1107 10-11 -0.08653920053770037 1107 12-13 -0.0400973949659118 1107 14-15 -0.07501487847383714 1107 16-17 -0.03822337924358976 1107 18-19 -0.014974766016358387 1107 20-21 -0.004991200833863729 1107 22-23 0.05533941811068388 1107 24-25 0.0321368024609896 1107 26-27 -0.11442381367007926 1107 28-29 -0.05486692626353573 1107 30-31 0.02484920641243349 1107 32-33 0.10281832760387033 1107 34-35 0.10307874733052103 1107 36-37 -0.06868194301211616 1107 38-39 -0.12097422463801877 1107 40-41 -0.06018718180126115 1107 42-43 -0.036329905058586576 1107 44-45 0.10957837993105812 1107 46-47 -0.042856644333951976 1107 48-49 0.017149644366860173 1107 50-51 0.08900160773679033 1107 52-53 0.09957640152814662 1107 54-55 0.147919826755043 1107 56-57 0.19683425361555607 1107 58-59 0.07589237669215976 1107 60-61 0.19054198677802248 1107 62-63 0.12005186454573646 1107 64-65 0.1616165180629281 1107 66-67 0.21363264539751725 1107 68-69 0.3950865161154695 1107 70-71 0.4379663227005963 1107 72-73 0.5005714673884292 1107 74-75 0.5974602201719001 1107 76-77 0.5816158549273247 1107 78-79 0.39454323154712156 1107 80-81 0.4489276203044561 1107 82-83 0.4429384067626927 1107 84-85 0.5273664279901382 1107 86-87 0.6103701193273849 1107 88-89 0.7516435982730307 1107 90-91 0.8367439822611864 1107 92-93 1.0071502355904478 1107 94-95 0.9494082175994123 1107 96-97 0.9360703374695483 1107 98-99 1.1975880364189102 1107 100-101 1.7793888342423934 1108 1 -0.06553874965890216 1108 2 -0.07795846145256036 1108 3 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0.9018440268762014 2315 96-97 0.9567494200222022 2315 98-99 1.033409950466396 2315 100-101 0.731079916958894 2316 1 -0.7577976643530988 2316 2 -0.5219321043715546 2316 3 -0.2619035413874755 2316 4 -0.16331381452406646 2316 5 -0.13481235885277698 2316 6 -0.26732006110333373 2316 7 -0.12007190038085014 2316 8 -0.03788059298330637 2316 9 -0.17815631373890284 2316 10-11 0.016614586084955363 2316 12-13 -0.13254952359621086 2316 14-15 -0.04670370017598202 2316 16-17 -0.12405827486394827 2316 18-19 -0.19543946060672823 2316 20-21 -0.15579140733876073 2316 22-23 -0.02756293220009809 2316 24-25 0.03553261751613235 2316 26-27 0.042799124769793195 2316 28-29 0.09877623599408736 2316 30-31 0.02087808586959028 2316 32-33 -0.08837095912468129 2316 34-35 -0.09546303136376366 2316 36-37 -0.05639490119487078 2316 38-39 -0.0863701119113287 2316 40-41 -0.4350021715589705 2316 42-43 -0.2432535772935367 2316 44-45 -0.28215381170470977 2316 46-47 -0.17502768816940062 2316 48-49 -0.6554535178126812 2316 50-51 -0.3626122241881262 2316 52-53 -0.1260872980130756 2316 54-55 -0.2364366459928533 2316 56-57 -0.0582474163415867 2316 58-59 -4.532477167984439E-4 2316 60-61 -0.028180571540580956 2316 62-63 0.12949360839963475 2316 64-65 -0.1531703456487179 2316 66-67 -0.010804711691037028 2316 68-69 0.0107598089610903 2316 70-71 0.24038153223573033 2316 72-73 0.2309522815603664 2316 74-75 0.3556315587355172 2316 76-77 0.11891751116302274 2316 78-79 0.05516784056873192 2316 80-81 0.12387494496315199 2316 82-83 0.139913929685612 2316 84-85 0.145921713432422 2316 86-87 -0.33975738476416595 2316 88-89 -0.19417941062834387 2316 90-91 -0.19512150067824763 2316 92-93 0.062103571923113776 2316 94-95 -0.15511107782458922 2316 96-97 0.18576326389583286 2316 98-99 0.19596722665813715 2316 100-101 -0.26116127296254277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 21.0 5 51.0 6 81.0 7 259.0 8 674.0 9 1240.0 10 2077.0 11 3280.0 12 4855.0 13 6672.0 14 8786.0 15 12062.0 16 15236.0 17 20039.0 18 17984.0 19 13579.0 20 12760.0 21 13053.0 22 14287.0 23 16401.0 24 19130.0 25 23164.0 26 27961.0 27 35288.0 28 44091.0 29 54279.0 30 67750.0 31 84880.0 32 107182.0 33 139097.0 34 187564.0 35 262936.0 36 386175.0 37 516501.0 38 329874.0 39 21224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.542121904135435 19.925366302962747 15.782074141161623 22.750437651740196 2 15.77642924393504 20.595368876446972 39.46849668609861 24.159705193519372 3 19.478956098800975 23.914532350105365 29.98981583500034 26.616695716093332 4 13.459167408081434 17.290251131146626 36.90772158856783 32.34285987220411 5 13.84867224003507 37.323042584738985 34.461364963878886 14.366920211347054 6 32.3972212934861 36.86971311903411 16.83305660145185 13.90000898602794 7 28.821267904255897 32.48453148388465 20.294296462824153 18.399904149035297 8 26.61147410526282 35.41162889161798 19.253212504988866 18.72368449813034 9 25.914490253397894 16.874465189665905 19.936069673289072 37.274974883647126 10-11 24.950915776714698 26.877814335851653 27.20748464328151 20.96378524415214 12-13 25.820543860334443 23.786689259878678 27.82747929096121 22.56528758882567 14-15 22.479985849029873 25.502954460267592 25.852842863515193 26.164216827187346 16-17 22.198342685008956 28.71141168879847 26.763528222199373 22.326717403993197 18-19 22.22094942647284 27.15500736287605 29.00200040315693 21.622042807494186 20-21 22.647805992466306 26.245192272663616 29.444886100071972 21.66211563479811 22-23 22.398386390370153 26.095917969176913 29.370557048289285 22.135138592163646 24-25 22.383349156834406 26.379108687921764 29.273524209754253 21.964017945489573 26-27 22.131729657843387 26.704868241805325 29.01066447708887 22.152737623262418 28-29 22.151924353556453 26.90217127313353 29.08415100144104 21.86175337186898 30-31 22.31128003829196 26.546387005829807 28.93908805632885 22.203244899549382 32-33 22.086624499798017 26.515845767378455 28.96741229110521 22.430117441718323 34-35 22.562236339011264 26.7209053058852 28.781316509761982 21.935541845341554 36-37 22.654747901438654 26.365188127757033 28.79414791001991 22.185916060784407 38-39 22.269383347153127 26.706154101786673 28.847591196970807 22.1768713540894 40-41 22.599332119004252 26.562335559603316 28.801113134992917 22.037219186399515 42-43 22.494197831185126 26.485397723339478 28.611442731085816 22.40896171438958 44-45 22.4853809868737 26.78364305827346 28.625801634771282 22.105174320081556 46-47 22.83977102629344 27.005139036519704 28.017848194415546 22.13724174277131 48-49 22.62845769807108 27.138654411127778 27.849258976515706 22.383628914285435 50-51 22.659402825822443 27.26411474267173 27.89246881807827 22.184013613427556 52-53 22.98688687562285 27.06049215908346 27.794472540398672 22.15814842489502 54-55 22.670331778723515 27.21483326060857 27.821086564592946 22.293748396074967 56-57 22.665595899133052 27.33636726462881 27.755775951608214 22.242260884629925 58-59 22.613845283266315 27.043774908905156 27.84622315626541 22.496156651563123 60-61 22.446490632268258 27.25381319262239 28.02365673049445 22.276039444614902 62-63 22.55205622177193 27.180386820168533 28.125903156524462 22.14165380153507 64-65 22.400467597273753 27.162515958295113 28.020985208674187 22.416031235756943 66-67 22.373610707459456 27.25666686365767 27.847822021597228 22.521900407285646 68-69 22.65857303495403 26.990465905309936 27.97619674058147 22.37476431915457 70-71 22.508866619011034 27.122928862231237 27.74415887945041 22.624045639307315 72-73 22.516840706868482 27.2154606634603 27.677127968323845 22.59057066134737 74-75 22.47180937315574 27.4281704689978 27.524547642543908 22.575472515302557 76-77 22.51032381273118 27.12576229446485 27.803134747512853 22.560779145291118 78-79 22.56015174243939 27.26686721969867 27.70509799222992 22.46788304563202 80-81 22.671438439296004 27.30578277559547 27.499124165326016 22.52365461978251 82-83 22.670928697935818 27.301738739561053 27.549786045031837 22.477546517471286 84-85 22.529592831539187 27.383572115393374 27.51038913963777 22.576445913429673 86-87 22.600265047346653 27.42309052977897 27.391295372357522 22.58534905051686 88-89 22.52748631654619 27.450230762816624 27.26162537006649 22.76065755057069 90-91 22.424383739316085 27.45840387126756 27.39009238546542 22.727120003950933 92-93 22.573727876374857 27.11916034254682 27.571570362478525 22.735541418599794 94-95 22.397715120246815 27.03661037311445 27.615723444061167 22.949951062577565 96-97 22.53005664435811 26.84219644428541 27.671036089021584 22.956710822334895 98-99 22.317515611218624 27.054534326870805 27.414016358638477 23.21393370327209 100-101 22.330753637533004 26.80875740435441 27.78422448717287 23.076264470939712 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 239.0 1 178.0 2 150.0 3 207.5 4 189.0 5 191.5 6 395.5 7 930.0 8 1570.5 9 1863.0 10 1716.5 11 1497.5 12 1514.0 13 1691.0 14 1904.5 15 2080.0 16 2210.0 17 2400.0 18 2709.5 19 3089.5 20 3474.0 21 4091.0 22 4986.5 23 6064.0 24 7523.0 25 9465.5 26 12346.0 27 15983.5 28 19271.5 29 22772.5 30 26938.5 31 32711.0 32 40749.5 33 45869.5 34 48617.0 35 52717.5 36 58462.5 37 64085.0 38 68701.0 39 73016.0 40 76435.5 41 81068.5 42 84177.5 43 85582.5 44 88709.0 45 92652.0 46 94465.5 47 95677.0 48 96201.5 49 94983.0 50 92331.0 51 89249.5 52 87829.5 53 86992.0 54 85355.5 55 78950.0 56 71038.0 57 64072.0 58 57966.5 59 50663.5 60 40675.0 61 33221.5 62 27389.0 63 20051.5 64 13695.5 65 9801.0 66 7089.0 67 5416.0 68 4048.0 69 3063.5 70 2339.0 71 1506.0 72 970.0 73 720.5 74 536.5 75 391.0 76 269.5 77 166.0 78 105.0 79 75.5 80 50.5 81 24.0 82 12.5 83 11.5 84 7.5 85 5.0 86 3.5 87 2.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.066545179886517 2 0.0 3 0.0 4 0.0 5 0.0025500890102497387 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.4167161168054102E-4 12-13 0.005808536078902182 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.47641653396759E-4 24-25 8.297908684145975E-4 26-27 4.250148350416232E-4 28-29 0.0025096114069124414 30-31 3.845372317043257E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.6549243837892053E-4 40-41 8.095520667459488E-5 42-43 0.01234566901787572 44-45 1.4167161168054102E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.0716405005946165E-5 82-83 2.2262681835513593E-4 84-85 5.464476450535154E-4 86-87 0.0 88-89 0.0 90-91 0.008095520667459487 92-93 0.002671521820261631 94-95 0.0 96-97 0.0 98-99 1.2143281001189233E-4 100-101 0.010099162032655712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2470502.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.30383812375869 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52686309068747 39.927908710244594 2 14.520066331393847 15.769906632125 3 5.039581541781537 8.210058607691604 4 2.387162715185394 5.185283906419995 5 1.2499110690568063 3.393748418157748 6 0.7868812240352655 2.563840236758169 7 0.4940899090978216 1.878168490956152 8 0.355584880891646 1.5447699048956764 9 0.26684011100746347 1.3041397973767583 >10 1.2699484625674102 12.137054420531031 >50 0.06910669134776161 2.546906573541159 >100 0.030080912412712147 2.9005758734835068 >500 0.002314901472614597 0.8519760683917762 >1k 0.001418810579985941 1.2413871195442687 >5k 1.4934848210378327E-4 0.5442752398825066 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7514 0.30414871147645295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5908 0.23914168051675327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4287 0.17352748550699412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.047760333729744E-5 0.0 5 0.0 0.0 0.0 4.047760333729744E-5 0.0 6 0.0 0.0 0.0 4.047760333729744E-5 0.0 7 4.047760333729744E-5 0.0 0.0 4.047760333729744E-5 0.0 8 4.047760333729744E-5 0.0 0.0 4.047760333729744E-5 0.0 9 4.047760333729744E-5 0.0 0.0 4.047760333729744E-5 0.0 10-11 4.047760333729744E-5 0.0 0.0 6.071640500594616E-5 0.0 12-13 4.047760333729744E-5 0.0 0.0 8.095520667459488E-5 2.023880166864872E-5 14-15 4.047760333729744E-5 0.0 0.0 1.2143281001189232E-4 4.047760333729744E-5 16-17 4.047760333729744E-5 0.0 0.0 1.4167161168054102E-4 4.047760333729744E-5 18-19 4.047760333729744E-5 0.0 0.0 2.023880166864872E-4 4.047760333729744E-5 20-21 4.047760333729744E-5 0.0 0.0 2.4286562002378463E-4 4.047760333729744E-5 22-23 4.047760333729744E-5 0.0 0.0 2.4286562002378463E-4 8.095520667459488E-5 24-25 4.047760333729744E-5 0.0 0.0 3.0358202502973083E-4 8.095520667459488E-5 26-27 4.047760333729744E-5 0.0 0.0 4.8573124004756927E-4 8.095520667459488E-5 28-29 4.047760333729744E-5 0.0 0.0 0.001153611695112977 8.095520667459488E-5 30-31 4.047760333729744E-5 0.0 0.0 0.003015581448628659 8.095520667459488E-5 32-33 4.047760333729744E-5 0.0 0.0 0.006435938930630293 8.095520667459488E-5 34-35 4.047760333729744E-5 0.0 0.0 0.010342027652679497 1.2143281001189232E-4 36-37 4.047760333729744E-5 0.0 0.0 0.015988653318232487 1.2143281001189232E-4 38-39 4.047760333729744E-5 0.0 0.0 0.02151384617377359 1.2143281001189232E-4 40-41 4.047760333729744E-5 0.0 0.0 0.026978322624308743 1.2143281001189232E-4 42-43 4.047760333729744E-5 0.0 0.0 0.034183336018347686 1.2143281001189232E-4 44-45 8.095520667459488E-5 0.0 0.0 0.044424169662683935 1.2143281001189232E-4 46-47 8.095520667459488E-5 0.0 0.0 0.05679007748222831 1.2143281001189232E-4 48-49 8.095520667459488E-5 0.0 0.0 0.07429664092560945 1.2143281001189232E-4 50-51 8.095520667459488E-5 0.0 0.0 0.09164129395564141 1.6191041334918976E-4 52-53 8.095520667459488E-5 0.0 0.0 0.11734457207482528 2.023880166864872E-4 54-55 8.095520667459488E-5 0.0 0.0 0.1539565642934108 2.4286562002378463E-4 56-57 8.095520667459488E-5 0.0 0.0 0.20756914991366127 2.4286562002378463E-4 58-59 1.2143281001189232E-4 0.0 0.0 0.2613638847489296 2.4286562002378463E-4 60-61 1.2143281001189232E-4 0.0 0.0 0.31817420103282656 2.4286562002378463E-4 62-63 1.2143281001189232E-4 0.0 0.0 0.38263478434747267 2.4286562002378463E-4 64-65 1.2143281001189232E-4 0.0 0.0 0.4550087391145605 2.4286562002378463E-4 66-67 1.2143281001189232E-4 0.0 0.0 0.5548062701426674 2.4286562002378463E-4 68-69 1.2143281001189232E-4 0.0 0.0 0.6776760350730338 2.4286562002378463E-4 70-71 1.2143281001189232E-4 0.0 0.0 0.8041483067004196 2.4286562002378463E-4 72-73 1.2143281001189232E-4 0.0 0.0 0.9442817694541433 2.6310442169243337E-4 74-75 1.6191041334918976E-4 0.0 0.0 1.0926726632886758 2.833432233610821E-4 76-77 2.023880166864872E-4 0.0 0.0 1.2615249856102122 2.833432233610821E-4 78-79 2.023880166864872E-4 0.0 0.0 1.4428646485613044 3.238208266983795E-4 80-81 2.226268183551359E-4 0.0 0.0 1.6434311730976132 3.238208266983795E-4 82-83 2.4286562002378463E-4 0.0 0.0 1.859399425703764 3.238208266983795E-4 84-85 2.8334322336108205E-4 0.0 0.0 2.1051591943661654 3.238208266983795E-4 86-87 3.238208266983795E-4 0.0 0.0 2.371178003498884 3.4405962836702824E-4 88-89 3.238208266983795E-4 0.0 0.0 2.665834716992741 3.64298430035677E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4430 0.0 46.887646 1 GTATCAA 7625 0.0 40.01989 1 ATCAACG 9870 0.0 30.510818 3 TATCAAC 9935 0.0 30.454628 2 TCAACGC 9945 0.0 30.280722 4 CAACGCA 10155 0.0 29.60776 5 AACGCAG 10540 0.0 28.570171 6 ACGCAGA 12055 0.0 24.826565 7 CGCAGAG 12215 0.0 24.229134 8 TACATGG 7680 0.0 21.95578 2 GTACATG 7925 0.0 21.711512 1 ACATGGG 7440 0.0 21.323343 3 GCAGAGT 13765 0.0 21.086685 9 GAGTACT 8435 0.0 20.781868 12-13 TAAGGTG 1425 0.0 20.666103 5 AGAGTAC 11925 0.0 20.496054 10-11 GTACTTT 8690 0.0 19.78577 14-15 GTAAGGT 1450 0.0 19.654636 4 AGTACTT 8910 0.0 19.487358 12-13 AGGTAAG 1480 0.0 19.256233 2 >>END_MODULE