FastQCFastQC Report
Thu 26 May 2016
SRR937215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796104
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.3007144795152392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.21755951483725744No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12790.1606574015455267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.14256931255212887No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9810.12322510626752285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37250.028.4531541
GGTATCA26900.027.9162241
GCACCGT1056.9018875E-527.1436126
AGGCGAC1351.3397968E-524.6349665
TACATGG28600.024.4165532
GTACATG30400.024.076811
ATCAACG44200.023.8596323
TCAACGC44200.023.7536534
CAACGCA44550.023.4618745
AACGCAG45800.022.9209426
TATCAAC46200.022.8267482
ACATGGG30100.022.7263153
GACCGTC1100.002874026321.5942297
GAGTACT24450.020.78658912-13
AAGGCGA1604.9808932E-520.7844454
AGACCGT1604.985727E-520.7818286
ACACGGC1150.00371896520.655358
AGTACTT26150.020.43427312-13
GTACTTT25050.020.28423914-15
CATGGGG15300.020.1828424
Produced by FastQC (version 0.11.5)