##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 796104 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807820335031604 33.0 31.0 34.0 30.0 34.0 2 31.930663581642598 33.0 31.0 34.0 30.0 34.0 3 31.965512043652588 34.0 31.0 34.0 30.0 34.0 4 35.579552420286795 37.0 35.0 37.0 33.0 37.0 5 35.59225176610091 37.0 35.0 37.0 33.0 37.0 6 35.628475676544774 37.0 35.0 37.0 33.0 37.0 7 35.57294273110046 37.0 35.0 37.0 33.0 37.0 8 35.55067177152734 37.0 35.0 37.0 33.0 37.0 9 37.1700619517048 39.0 37.0 39.0 34.0 39.0 10-11 37.06798923256258 39.0 37.0 39.0 33.0 39.0 12-13 36.93709176188035 39.0 37.0 39.0 33.0 39.0 14-15 38.08570613889643 40.0 38.0 41.0 33.0 41.0 16-17 38.013427893843016 40.0 38.0 41.0 33.0 41.0 18-19 37.94878056133369 40.0 38.0 41.0 33.0 41.0 20-21 37.82987838272386 40.0 38.0 41.0 33.0 41.0 22-23 37.88907793454122 40.0 38.0 41.0 33.0 41.0 24-25 37.86168641283048 40.0 38.0 41.0 33.0 41.0 26-27 37.78184822585994 40.0 38.0 41.0 33.0 41.0 28-29 37.663482409333454 40.0 37.5 41.0 33.0 41.0 30-31 37.43285550631576 40.0 37.0 41.0 32.0 41.0 32-33 37.20612633525268 39.5 36.5 41.0 31.5 41.0 34-35 37.23647412900827 40.0 37.0 41.0 31.5 41.0 36-37 37.12784761789917 40.0 37.0 41.0 31.0 41.0 38-39 36.99008735039643 40.0 36.0 41.0 31.0 41.0 40-41 36.78783550892848 39.5 36.0 41.0 30.5 41.0 42-43 36.54632560569976 39.0 36.0 41.0 30.0 41.0 44-45 36.31304892325626 39.0 36.0 41.0 30.0 41.0 46-47 35.95719654718479 39.0 35.0 40.5 29.0 41.0 48-49 35.64906532312361 39.0 35.0 40.0 27.5 41.0 50-51 34.698604704912924 38.0 34.0 39.5 26.0 40.5 52-53 34.79986471616773 38.0 34.5 39.5 26.5 40.5 54-55 35.11206073578326 39.0 35.0 40.0 26.0 41.0 56-57 34.87639805854512 38.0 34.5 40.0 26.0 41.0 58-59 34.627787952327836 38.0 34.0 40.0 25.0 41.0 60-61 34.3100637605137 38.0 34.0 40.0 24.0 41.0 62-63 33.963522981921955 38.0 33.5 40.0 23.0 41.0 64-65 33.552315401002886 37.0 33.0 40.0 22.0 41.0 66-67 33.11680634690945 36.5 32.5 39.0 21.0 41.0 68-69 32.63149538251284 36.0 32.0 39.0 19.0 40.0 70-71 32.1652114547848 35.5 31.0 39.0 17.5 40.0 72-73 31.66978937425261 35.0 31.0 38.0 13.5 40.0 74-75 31.13357111633656 35.0 30.5 37.0 8.0 39.0 76-77 30.607275556962406 34.5 30.0 37.0 7.0 39.0 78-79 30.06271165576357 34.0 29.5 36.0 2.0 38.5 80-81 29.50342857214635 34.0 29.0 36.0 2.0 37.5 82-83 28.956008260227307 34.0 29.0 35.0 2.0 37.0 84-85 28.41063981590345 34.0 27.5 35.0 2.0 36.5 86-87 27.85020612884749 33.5 26.5 35.0 2.0 36.0 88-89 27.298957799483485 33.0 26.0 35.0 2.0 35.5 90-91 26.724544783093663 32.5 25.0 35.0 2.0 35.0 92-93 26.050614618190586 32.0 24.5 34.5 2.0 35.0 94-95 25.28777458723986 32.0 20.5 34.0 2.0 35.0 96-97 24.418954684312602 31.5 12.5 34.0 2.0 35.0 98-99 23.230196054786813 31.0 2.0 34.0 2.0 35.0 100-101 20.102626038809 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.038093936394190564 1101 2 0.01847507587320152 1101 3 0.07263644210914677 1101 4 0.03913314127092349 1101 5 0.15543777873914166 1101 6 0.171380257830414 1101 7 0.0815267883931412 1101 8 0.05346111327331471 1101 9 0.05973598109155631 1101 10-11 0.2568538796738835 1101 12-13 0.22292772606400746 1101 14-15 0.4649801943430347 1101 16-17 0.4153514826647182 1101 18-19 0.3414218432751923 1101 20-21 0.24211125619639517 1101 22-23 0.3310248303891683 1101 24-25 0.3795010491095425 1101 26-27 0.2846950001799158 1101 28-29 0.259788214125642 1101 30-31 0.2975604680944457 1101 32-33 0.30859931683131947 1101 34-35 0.44560936859509326 1101 36-37 0.4566496909877742 1101 38-39 0.4913053866779009 1101 40-41 0.6227343066416466 1101 42-43 0.5998763879949678 1101 44-45 0.897422399114248 1101 46-47 0.8842291805903386 1101 48-49 0.8164138830124017 1101 50-51 0.6806224927977809 1101 52-53 0.7566967478575748 1101 54-55 0.8995970147121426 1101 56-57 0.8844995968911107 1101 58-59 1.0468662835296954 1101 60-61 1.0143333463800133 1101 62-63 0.9792856736739353 1101 64-65 0.8552916236460604 1101 66-67 1.0396192410824696 1101 68-69 0.8865812059283869 1101 70-71 0.8372282027794 1101 72-73 0.949579624297467 1101 74-75 1.178984724748208 1101 76-77 0.9562813544640036 1101 78-79 1.1201571865805882 1101 80-81 1.2149107406814252 1101 82-83 1.0754456235579823 1101 84-85 1.136581482904564 1101 86-87 1.3378536749997743 1101 88-89 1.514356475901888 1101 90-91 1.3437266859103367 1101 92-93 1.6757422911049709 1101 94-95 1.798118302858704 1101 96-97 1.861531005364295 1101 98-99 1.9099894767783674 1101 100-101 2.0152770633520447 1102 1 0.09233428555875989 1102 2 0.12378612550853774 1102 3 -0.026679957442745206 1102 4 0.0888690748770884 1102 5 0.11884800759668224 1102 6 0.06737239299582853 1102 7 -0.0075241729914381494 1102 8 0.013670537357086232 1102 9 0.14698077406807641 1102 10-11 0.15195664837001743 1102 12-13 0.1643324221018645 1102 14-15 0.2522204147413092 1102 16-17 0.26282856261624943 1102 18-19 0.32331671970126763 1102 20-21 0.28229964642021343 1102 22-23 0.24474485142302882 1102 24-25 0.1603739542971283 1102 26-27 0.40384267159444676 1102 28-29 0.29913982253003724 1102 30-31 0.24963298918546428 1102 32-33 0.196719758542379 1102 34-35 0.32112549836486437 1102 36-37 0.11602461954124266 1102 38-39 0.02798660938533004 1102 40-41 0.15557227733141588 1102 42-43 0.34916649522192955 1102 44-45 0.21559382507277292 1102 46-47 0.2794165419840624 1102 48-49 0.24807898738149703 1102 50-51 0.1947403281392326 1102 52-53 0.18964903300355473 1102 54-55 0.5046702902785754 1102 56-57 0.3908417629214185 1102 58-59 0.4664460635886769 1102 60-61 0.6276989925996403 1102 62-63 0.2524880788500923 1102 64-65 0.21002081269930528 1102 66-67 0.4302408830953155 1102 68-69 0.48020017298295414 1102 70-71 0.5898471469710884 1102 72-73 0.561909581543862 1102 74-75 0.569322866909669 1102 76-77 0.7499620513249283 1102 78-79 0.773240247464468 1102 80-81 0.7477098444560895 1102 82-83 0.7075634131736557 1102 84-85 0.4601234020156575 1102 86-87 0.5512536155562593 1102 88-89 0.6419817073886627 1102 90-91 0.48179111353785586 1102 92-93 0.7394497375486502 1102 94-95 0.7905552584361075 1102 96-97 0.5280528737197692 1102 98-99 1.0256345532547364 1102 100-101 1.2355815970633763 1103 1 0.15804109184148274 1103 2 2.2591608445310385E-4 1103 3 0.09138286978238952 1103 4 0.013738184824731547 1103 5 0.05837680340820128 1103 6 0.09302684325760424 1103 7 0.11891561758447722 1103 8 0.07126216039372935 1103 9 0.030750407575929728 1103 10-11 0.06373675308205407 1103 12-13 -0.0666885203065064 1103 14-15 0.033895807411461476 1103 16-17 0.004451599265458128 1103 18-19 0.09360467781644388 1103 20-21 0.39499598149875226 1103 22-23 0.03898568911936451 1103 24-25 0.12778233126047667 1103 26-27 0.08394738363632825 1103 28-29 -0.18777117223435624 1103 30-31 -0.06738271762082348 1103 32-33 0.33781923498217026 1103 34-35 0.20790560307690242 1103 36-37 0.31065812739463894 1103 38-39 0.2603164523172623 1103 40-41 0.30740473806440605 1103 42-43 0.09890471511721444 1103 44-45 0.21664094549161916 1103 46-47 0.2731338194709778 1103 48-49 0.30776485125584685 1103 50-51 0.2713110087675119 1103 52-53 0.03938725289884104 1103 54-55 0.17076976671835808 1103 56-57 0.07801453779052991 1103 58-59 0.03450889081381092 1103 60-61 0.1776989925996375 1103 62-63 0.08625771235794844 1103 64-65 0.12481138861553376 1103 66-67 0.40969114487543123 1103 68-69 0.4236556703651573 1103 70-71 0.3228314401648049 1103 72-73 0.3149200527480538 1103 74-75 0.3089825527735428 1103 76-77 0.14603534975424637 1103 78-79 0.5057009804487613 1103 80-81 0.6054323575450979 1103 82-83 0.47052152835690464 1103 84-85 0.5508302082983789 1103 86-87 0.5920913118913411 1103 88-89 0.6976099796399744 1103 90-91 0.46176493552738407 1103 92-93 0.717983768962263 1103 94-95 0.3629374573889841 1103 96-97 0.5725554915208129 1103 98-99 1.0832261762913866 1103 100-101 1.1588800263827501 1104 1 0.23955449903247583 1104 2 0.19886060990297239 1104 3 -0.011637002364789595 1104 4 0.058060015260650744 1104 5 0.029913640641723305 1104 6 0.09245555518378978 1104 7 0.1073018372483574 1104 8 0.0971075221130917 1104 9 0.041163759807567146 1104 10-11 0.2740466949408358 1104 12-13 0.15696352909905897 1104 14-15 0.2150628260108718 1104 16-17 0.42670941651113026 1104 18-19 0.34709387396350166 1104 20-21 0.41761971237144735 1104 22-23 0.42764959817608883 1104 24-25 0.36496089132527487 1104 26-27 0.4061032368080646 1104 28-29 0.482422053301498 1104 30-31 0.4089956052509507 1104 32-33 0.18205645693119976 1104 34-35 0.3112653554705247 1104 36-37 0.37752775800989724 1104 38-39 0.35947832209250663 1104 40-41 0.4898553469562472 1104 42-43 0.424184573958442 1104 44-45 0.16869975200575027 1104 46-47 0.3576193709447537 1104 48-49 -0.024243913315608268 1104 50-51 0.015048534439316086 1104 52-53 -0.08178005496034046 1104 54-55 0.2630183276509328 1104 56-57 0.24378208862793826 1104 58-59 0.2368135681445196 1104 60-61 0.21962358257389525 1104 62-63 0.34341327626989226 1104 64-65 0.050218521761813406 1104 66-67 0.4838718165366416 1104 68-69 0.2433925885914121 1104 70-71 0.16588631274586874 1104 72-73 0.46948963418914147 1104 74-75 0.2725177412262916 1104 76-77 0.5566251504542556 1104 78-79 0.6639803186223752 1104 80-81 0.4398383625782145 1104 82-83 0.7108855617953331 1104 84-85 0.31313459636930574 1104 86-87 0.5077214643312438 1104 88-89 0.590551317755736 1104 90-91 0.7371077083716777 1104 92-93 1.0264648658353615 1104 94-95 1.005593151467835 1104 96-97 1.0167247374205566 1104 98-99 1.2831697706334673 1104 100-101 0.9447699341088764 1105 1 0.1701484216844129 1105 2 -0.09855041412375343 1105 3 0.040054183553237976 1105 4 0.0833153795160797 1105 5 0.1997228401791773 1105 6 0.2344718085565205 1105 7 0.1689506230636013 1105 8 -0.08723656114528922 1105 9 0.25215245311348866 1105 10-11 0.11977215427528165 1105 12-13 -0.021040614547345626 1105 14-15 0.12468556145992693 1105 16-17 0.1716141768863082 1105 18-19 0.19760139034536195 1105 20-21 0.22489598758666318 1105 22-23 0.22248288868103572 1105 24-25 0.12351318478538076 1105 26-27 0.3238566737860964 1105 28-29 0.09946400370622399 1105 30-31 0.12572196398151902 1105 32-33 0.08168947119305159 1105 34-35 0.2827336196360193 1105 36-37 0.1946492086073519 1105 38-39 0.36083544659463485 1105 40-41 0.31900220549408687 1105 42-43 0.5197695026491758 1105 44-45 0.4798707336323744 1105 46-47 0.07257905872582171 1105 48-49 0.0041874434876731925 1105 50-51 0.004283887131080633 1105 52-53 0.06957947863852354 1105 54-55 0.22897779058296663 1105 56-57 0.15334861181950998 1105 58-59 -0.05293251305810287 1105 60-61 0.11799182104234518 1105 62-63 0.15209084276096263 1105 64-65 0.1566283251795184 1105 66-67 0.15655587152829042 1105 68-69 0.07740582256288775 1105 70-71 0.30220103714519553 1105 72-73 0.23145481471079066 1105 74-75 0.1165247115462229 1105 76-77 0.15055181754921065 1105 78-79 0.34069961066914445 1105 80-81 0.44091041063166614 1105 82-83 0.42254880219106994 1105 84-85 0.510242877237868 1105 86-87 0.5749995062174058 1105 88-89 0.9298485039307316 1105 90-91 0.724572374952686 1105 92-93 0.7363654504428432 1105 94-95 0.7131368364222226 1105 96-97 1.0931212105029182 1105 98-99 1.295351769862556 1105 100-101 1.550115719795631 1106 1 0.1972501915959164 1106 2 -0.7463894756590506 1106 3 -0.016523816035153516 1106 4 0.15332721322671716 1106 5 0.15099367780008777 1106 6 0.024384856508774533 1106 7 0.09992150184524462 1106 8 0.16850536106092306 1106 9 0.16793244899741921 1106 10-11 -0.05979797332298631 1106 12-13 -0.3370251278216827 1106 14-15 -0.14887767171562416 1106 16-17 1.9130180913151662E-4 1106 18-19 0.008879431094484858 1106 20-21 0.1749976031454139 1106 22-23 0.15416893519266495 1106 24-25 0.12533711975142126 1106 26-27 0.19783794565272927 1106 28-29 0.2531916225578428 1106 30-31 0.17974928441781657 1106 32-33 0.1494927743418515 1106 34-35 0.1357666511239799 1106 36-37 0.08438860971869389 1106 38-39 0.40586271558002096 1106 40-41 0.45327880561757183 1106 42-43 0.16598528779014998 1106 44-45 0.25461991041772336 1106 46-47 0.3831046298309815 1106 48-49 0.5081633130081471 1106 50-51 0.17736090915207114 1106 52-53 0.4651482685129835 1106 54-55 0.5286150616263896 1106 56-57 0.35704664639452943 1106 58-59 0.16405529307484557 1106 60-61 0.2977355954815337 1106 62-63 0.3294189967021097 1106 64-65 0.41299537601302205 1106 66-67 0.6539678904622335 1106 68-69 0.6615280699388961 1106 70-71 0.6376532906953116 1106 72-73 0.59210000919526 1106 74-75 0.3472036063805497 1106 76-77 0.6373688066416143 1106 78-79 1.0321060214532558 1106 80-81 0.8761349938792478 1106 82-83 0.8603086293159912 1106 84-85 0.635089810763283 1106 86-87 0.6340085615785931 1106 88-89 0.8595974234614978 1106 90-91 0.9319324284616393 1106 92-93 1.2021750306033674 1106 94-95 1.1760021379245913 1106 96-97 1.4867425832133492 1106 98-99 1.715353313390029 1106 100-101 1.2966620770707884 1107 1 0.0687139954549032 1107 2 0.16504724758675593 1107 3 0.11478572504517004 1107 4 0.376403764317196 1107 5 6.278172193674436E-5 1107 6 0.25131797706586667 1107 7 0.1968846045097834 1107 8 -0.08205801240974608 1107 9 0.24390807644554968 1107 10-11 0.0962661497003694 1107 12-13 0.13896621605374548 1107 14-15 0.09049681447809377 1107 16-17 0.3321452164084846 1107 18-19 0.5465021401229748 1107 20-21 0.277241425380538 1107 22-23 0.3952891537718983 1107 24-25 0.2862165143062825 1107 26-27 0.21972971991647228 1107 28-29 0.34375330349018185 1107 30-31 0.12936362747115737 1107 32-33 0.26935229366096536 1107 34-35 0.28709408475229736 1107 36-37 0.11958010834048594 1107 38-39 0.29369699189899023 1107 40-41 0.41283560236789185 1107 42-43 0.37636659249540827 1107 44-45 0.5142262743618176 1107 46-47 0.2731803104731796 1107 48-49 0.3902792277042195 1107 50-51 0.1403994990258539 1107 52-53 0.2835401153140893 1107 54-55 0.5460844750409635 1107 56-57 0.47297705889029373 1107 58-59 0.7896658158273979 1107 60-61 0.5920196517705207 1107 62-63 0.5781217869724316 1107 64-65 0.49748326227445006 1107 66-67 0.7765838293374188 1107 68-69 1.0487808384480104 1107 70-71 0.7791120169393295 1107 72-73 1.159765273473024 1107 74-75 1.1018508330356092 1107 76-77 1.0263818150075856 1107 78-79 1.0974706425664635 1107 80-81 1.0778202784674775 1107 82-83 0.8064762389682976 1107 84-85 0.8478612213730798 1107 86-87 0.8519724379749363 1107 88-89 0.968910011110804 1107 90-91 0.9249869765539067 1107 92-93 1.119311981132892 1107 94-95 0.8786836022120355 1107 96-97 0.7031058019376601 1107 98-99 1.0642471186997646 1107 100-101 0.6812672320589428 1108 1 -0.014499867288972013 1108 2 -0.04525215609917055 1108 3 0.059844837015081254 1108 4 0.08099974594988879 1108 5 0.026221646439680057 1108 6 0.020952708724529145 1108 7 0.008972950413500769 1108 8 -0.22904839243010855 1108 9 0.003865690358161089 1108 10-11 -0.13712224399158401 1108 12-13 0.054648548950751774 1108 14-15 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1.3779442235128023 2311 90-91 1.340198428205131 2311 92-93 0.966722292370715 2311 94-95 1.3091302641269777 2311 96-97 1.7861718508804039 2311 98-99 2.2743920462359988 2311 100-101 2.246410377699238 2312 1 0.22850763493493176 2312 2 0.1256418917166826 2312 3 0.12643593038746204 2312 4 0.020363985611702162 2312 5 0.12335301014402944 2312 6 0.05538579093410334 2312 7 0.08129895471172688 2312 8 0.27747767642031107 2312 9 0.3619374692433013 2312 10-11 0.10922307872999681 2312 12-13 0.2507632156803936 2312 14-15 0.2569725637229183 2312 16-17 0.3149753762699987 2312 18-19 0.3051655484656379 2312 20-21 0.3429339889348242 2312 22-23 0.39951055009949954 2312 24-25 0.2623348661373939 2312 26-27 0.13165314930247973 2312 28-29 -0.00869298507513605 2312 30-31 0.05921208146430246 2312 32-33 0.237689699899299 2312 34-35 -0.29468645354998557 2312 36-37 -0.2094130356027648 2312 38-39 -0.23081256245732362 2312 40-41 -0.036876748716515806 2312 42-43 -0.16790503632718412 2312 44-45 -0.013113100447256443 2312 46-47 -0.08219337856052533 2312 48-49 0.1406483347197991 2312 50-51 0.08844026201672506 2312 52-53 0.20034678809010842 2312 54-55 0.28624162129219854 2312 56-57 0.2592471264326335 2312 58-59 0.11200230566210223 2312 60-61 -0.19617231781845845 2312 62-63 0.009200597684170475 2312 64-65 0.4117952807382821 2312 66-67 0.4551679857369706 2312 68-69 0.6244350488135524 2312 70-71 0.48760656942889113 2312 72-73 0.7016567984365416 2312 74-75 0.5452119978864403 2312 76-77 0.7021446361731201 2312 78-79 0.5299495143717792 2312 80-81 0.4240087182054566 2312 82-83 0.5741509496223394 2312 84-85 1.15081747158521 2312 86-87 1.1206865879257215 2312 88-89 1.2314638922498595 2312 90-91 1.4980659230291486 2312 92-93 1.7035235346067417 2312 94-95 2.0575774690959214 2312 96-97 2.247351975104003 2312 98-99 2.1110897688032857 2312 100-101 2.856529425318282 2313 1 0.17154862535040039 2313 2 0.04587482151305622 2313 3 0.013272698212453804 2313 4 0.06584095617500907 2313 5 0.07732787025543075 2313 6 0.08916145474607617 2313 7 0.23971567303281205 2313 8 0.20295194830386265 2313 9 0.19053757128712334 2313 10-11 0.3377021618535565 2313 12-13 0.38839166651172263 2313 14-15 0.20092857524290508 2313 16-17 0.3173641295221188 2313 18-19 0.32512945362391577 2313 20-21 0.5320186726622111 2313 22-23 0.42097137918451466 2313 24-25 0.46331271587996525 2313 26-27 0.21366982208334662 2313 28-29 0.40357462275609635 2313 30-31 0.3791869500090783 2313 32-33 0.18057256454785175 2313 34-35 0.3176439829193072 2313 36-37 0.1549002979835663 2313 38-39 0.2846057539881812 2313 40-41 0.20209556029918474 2313 42-43 0.29489324157779606 2313 44-45 0.19234497935775607 2313 46-47 0.1565217125410001 2313 48-49 -0.02511174421658069 2313 50-51 0.2079306332116886 2313 52-53 0.26278538258396367 2313 54-55 0.04799036577360738 2313 56-57 0.23816158250107833 2313 58-59 0.2793879174823246 2313 60-61 0.5714861136582741 2313 62-63 0.5753519851850726 2313 64-65 0.44541071043978064 2313 66-67 0.3838743482961675 2313 68-69 0.5684184687998055 2313 70-71 0.39352806314813904 2313 72-73 0.39193058793108193 2313 74-75 0.4309355603123812 2313 76-77 0.5528528287745189 2313 78-79 0.6590476071641937 2313 80-81 0.7460645218427686 2313 82-83 0.943939651430874 2313 84-85 1.0005495011188863 2313 86-87 1.2328820904072408 2313 88-89 1.5600408025145427 2313 90-91 1.3869456757626146 2313 92-93 1.7891602332780643 2313 94-95 1.283007887433449 2313 96-97 1.2210503892079778 2313 98-99 1.5858866299013918 2313 100-101 2.228875547863648 2314 1 0.10823162541691644 2314 2 0.07694417880908233 2314 3 0.2037875012139132 2314 4 0.28876810836703015 2314 5 0.2851463636339133 2314 6 0.24150868149948934 2314 7 0.35291975395441 2314 8 0.4254673341131294 2314 9 0.39281305566692737 2314 10-11 0.2852177852203468 2314 12-13 0.46151187378858083 2314 14-15 0.37695395272181287 2314 16-17 0.5062001926554132 2314 18-19 0.6015038822980614 2314 20-21 0.3947405118009186 2314 22-23 0.1813851093761798 2314 24-25 0.481001249453179 2314 26-27 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2.16845725303968 2314 94-95 1.9739487102693793 2314 96-97 2.382217335136719 2314 98-99 3.2059656256982088 2314 100-101 3.6666988240670335 2315 1 -0.029801107252328762 2315 2 0.020146571697399196 2315 3 0.10853246143360806 2315 4 -0.04666325831296092 2315 5 0.06286018407100613 2315 6 0.14014373882038456 2315 7 -0.07289380086203323 2315 8 0.0644974524388573 2315 9 0.09933468103924525 2315 10-11 0.02672640166957052 2315 12-13 -0.12379128749485346 2315 14-15 -0.07118617943962846 2315 16-17 0.08195859298696462 2315 18-19 -0.015394321570497027 2315 20-21 0.20473592780388117 2315 22-23 0.26318643182792556 2315 24-25 -0.04337975396074256 2315 26-27 0.00450786787666857 2315 28-29 0.10033844422078886 2315 30-31 -0.06864933871965917 2315 32-33 0.0550562041111462 2315 34-35 -0.09312125845093533 2315 36-37 -0.20197020827580303 2315 38-39 -0.052767091825977275 2315 40-41 -0.2590831805899825 2315 42-43 -0.43088818616737257 2315 44-45 -0.3763737888878893 2315 46-47 -0.18812061106040545 2315 48-49 -0.26428633503434895 2315 50-51 -0.23051317224479817 2315 52-53 -0.19115540351741345 2315 54-55 -0.3004264943711803 2315 56-57 -0.006193744748770769 2315 58-59 0.09861859182780819 2315 60-61 -0.005513609487557858 2315 62-63 0.36937659505368714 2315 64-65 0.3686165720730017 2315 66-67 -0.05548910640528959 2315 68-69 0.09733602382114981 2315 70-71 0.06666745236946525 2315 72-73 -0.14989906177986612 2315 74-75 0.15817801055401404 2315 76-77 -0.014980554356018416 2315 78-79 0.20704617081187138 2315 80-81 0.3007994134173586 2315 82-83 0.2244244477511792 2315 84-85 0.34442293764664456 2315 86-87 0.6248719591893312 2315 88-89 0.6638542631094246 2315 90-91 0.8177814738290188 2315 92-93 0.6988769922751352 2315 94-95 1.1821479948309381 2315 96-97 1.4243942941675094 2315 98-99 2.0415351402340853 2315 100-101 2.5391852133635027 2316 1 -0.016204789324760327 2316 2 0.04186456347385814 2316 3 -0.09992074710603305 2316 4 -0.06119336808366427 2316 5 -0.05235738171116111 2316 6 0.13868825831116283 2316 7 0.028895683870082678 2316 8 0.00277303987881794 2316 9 -0.12906154304609885 2316 10-11 -0.1293410844701839 2316 12-13 -0.10733648610697344 2316 14-15 -0.29855291731114164 2316 16-17 -0.31593782866094955 2316 18-19 -0.03893050639028672 2316 20-21 0.028855076556155268 2316 22-23 -0.1359686794862327 2316 24-25 -0.008727587563669204 2316 26-27 -0.19237172390966606 2316 28-29 -0.01310800397460099 2316 30-31 0.048587069592777254 2316 32-33 0.17535946265881108 2316 34-35 0.2549839930091764 2316 36-37 0.08380405179062933 2316 38-39 0.09335358186239517 2316 40-41 0.0790166070302547 2316 42-43 0.24035303155892507 2316 44-45 0.18747287786109723 2316 46-47 0.01447286073135956 2316 48-49 -0.1498489870649209 2316 50-51 -0.15295288479126157 2316 52-53 -0.10567254824434258 2316 54-55 0.09935063035253933 2316 56-57 0.13312645478942642 2316 58-59 0.06419331369674808 2316 60-61 -0.058200306117882405 2316 62-63 0.12658145509295338 2316 64-65 0.15040099426422415 2316 66-67 0.16553016211928906 2316 68-69 -0.009110072465908559 2316 70-71 -0.0324479920845846 2316 72-73 -0.09850038051003196 2316 74-75 -0.01609610134048367 2316 76-77 0.19991041579968893 2316 78-79 0.2560972530843344 2316 80-81 0.11736256552818958 2316 82-83 0.64665270660754 2316 84-85 0.6488776649485537 2316 86-87 0.8799653692427967 2316 88-89 0.7688579982767969 2316 90-91 0.9951479030090127 2316 92-93 1.2582144476692143 2316 94-95 1.2505941652039603 2316 96-97 1.3842389297262976 2316 98-99 1.5980773939291204 2316 100-101 1.8428736139710082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3105.0 3 583.0 4 516.0 5 587.0 6 650.0 7 747.0 8 917.0 9 1143.0 10 1498.0 11 1922.0 12 2629.0 13 3371.0 14 3972.0 15 5024.0 16 6299.0 17 8062.0 18 6889.0 19 5244.0 20 5149.0 21 5516.0 22 6130.0 23 7083.0 24 7933.0 25 8776.0 26 10482.0 27 12193.0 28 14588.0 29 17654.0 30 21357.0 31 26392.0 32 33688.0 33 44197.0 34 59031.0 35 80979.0 36 114149.0 37 150500.0 38 106992.0 39 10155.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.164322951443395 20.000025196723417 15.137435527883955 22.698216323949232 2 14.759759116862575 21.040502318412045 40.57762587554371 23.62211268918167 3 18.609893424239026 25.140948742134007 30.185887369456342 26.063270464170625 4 12.064199676357065 17.411863969222438 38.51111656812558 32.01281978629492 5 12.95531365072009 38.39809688842769 34.75533581506873 13.891253645783486 6 30.751198533558938 37.7841481347192 17.852988276528887 13.611665055192972 7 27.65855390379502 33.413529328821205 21.123398598017886 17.804518169365885 8 25.698231884495875 35.67126447200778 20.112250910244594 18.51825273325175 9 25.68520057414822 17.559744151494545 20.88564881267155 35.86940646168568 10-11 23.965499764210193 27.70980413390846 27.848696608089636 20.475999493791708 12-13 25.101596649183545 24.989533420339725 28.10186925243124 21.80700067804549 14-15 22.05147412916689 26.65468520331242 26.574487037042864 24.719353630477823 16-17 21.219369568132077 30.018814633995884 27.098423385287067 21.66339241258497 18-19 21.07466677072542 28.786049749314913 29.357344890568378 20.781938589391295 20-21 22.004395969605874 27.769951810505006 29.41743331772336 20.808218902165756 22-23 21.563378623935268 27.70067794284535 29.252815215728827 21.48312821749056 24-25 21.291712588252846 27.967866743791852 29.48508867613024 21.255331991825066 26-27 21.091127664328447 28.205801178866007 29.31064976098539 21.392421395820154 28-29 20.975688671982454 28.634565885329295 29.24170386396584 21.14804157872241 30-31 21.347395034587656 28.200208004391765 29.08026048395017 21.37213647707041 32-33 21.145904677490833 28.171709439587445 29.12314673059103 21.559239152330687 34-35 21.626070611578406 28.30380377922897 28.984412474552713 21.08571313463991 36-37 21.553991195438847 28.091006481939573 28.98499548693856 21.37000683568302 38-39 21.186900441695336 28.58369239173807 28.94268785897973 21.286719307586864 40-41 21.62451492880168 28.432567079226303 28.780156859092404 21.162761132879616 42-43 21.294871923985237 28.454112179180534 28.492192574763457 21.758823322070768 44-45 21.523773198238175 28.506249346993904 28.575294906993047 21.39468254777487 46-47 21.710497741145396 28.635553622381956 28.14173407770309 21.512214558769564 48-49 21.549842721865407 28.69676007672227 28.102269501135286 21.65112770027704 50-51 21.642064945521987 28.804694420119375 27.99975321683741 21.55348741752123 52-53 21.706890146769002 28.85046687780583 27.85947702130071 21.583165954124457 54-55 21.5603156537679 28.78716536067522 27.935710976371293 21.716808009185584 56-57 21.619013992760998 28.99603686340112 27.743466677743562 21.64148246609432 58-59 21.754381327981786 28.641226677440244 27.849192579573124 21.75519941500485 60-61 21.48818997237939 28.820079658609554 28.11556225146017 21.576168117550885 62-63 21.240581629236534 28.74538364520214 28.37764866109977 21.636386064461558 64-65 21.270320138144218 28.64604572251338 28.266891704802322 21.81674243454008 66-67 21.27752366903378 28.628077100257016 27.979743183746216 22.11465604696299 68-69 21.374821661987557 28.41721434608556 28.1245732317082 22.083390760218684 70-71 21.409942868219296 28.30295073878605 28.150901408344335 22.136204984650316 72-73 21.202764669527337 28.22954913632399 28.3637295094864 22.203956684662277 74-75 21.068647615786837 28.69086670510074 27.90212906295551 22.33835661615691 76-77 21.241189525748176 28.39525055799146 28.17545290778696 22.188107008473413 78-79 21.2811933113534 28.37217587469677 27.919925575399596 22.42670523855023 80-81 21.27019853460902 28.368551936661273 27.787858818434895 22.57339071029481 82-83 21.36103135632369 28.117286320456063 28.1231394337459 22.398542889474342 84-85 21.222498472443256 28.130578885799167 28.25045512066597 22.39646752109161 86-87 21.172740722467893 28.20504429918928 28.026639296549387 22.595575681793438 88-89 21.173770260693782 27.929013149862108 27.86846865217375 23.02874793727036 90-91 21.186205472925813 27.752621386841238 28.3804577742898 22.68071536594315 92-93 21.05270776663222 28.03721377528992 28.2930553335255 22.617023124552357 94-95 20.87659376823169 28.263735876439576 27.908337911906365 22.951332443422366 96-97 20.980256896994476 28.444594941616607 27.733306982341478 22.841841179047435 98-99 21.281908291435144 28.570493437699053 27.396001440105945 22.75159683075986 100-101 21.806958078089266 27.73375993233489 27.446787354578532 23.012494634997314 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1121.0 1 619.0 2 104.5 3 95.5 4 85.5 5 75.5 6 103.5 7 175.5 8 283.5 9 361.0 10 378.0 11 396.5 12 440.0 13 508.0 14 588.5 15 670.5 16 725.5 17 792.5 18 882.0 19 989.0 20 1182.0 21 1360.0 22 1572.0 23 1949.0 24 2457.0 25 3231.5 26 4380.5 27 5747.0 28 7240.0 29 8962.0 30 10804.0 31 12783.0 32 15286.5 33 17546.5 34 19706.5 35 21908.5 36 24525.0 37 27010.5 38 27986.0 39 28221.5 40 28478.0 41 29354.0 42 29944.5 43 30341.5 44 31136.5 45 31408.5 46 31154.5 47 31585.5 48 32057.5 49 31123.0 50 29169.0 51 26913.0 52 24845.5 53 23384.5 54 22384.5 55 20357.0 56 17449.0 57 15003.5 58 13062.5 59 10810.0 60 8467.5 61 6639.5 62 5088.0 63 3710.5 64 2593.0 65 1893.5 66 1371.5 67 989.0 68 725.0 69 534.0 70 388.0 71 261.5 72 197.5 73 163.5 74 121.0 75 82.0 76 62.0 77 44.5 78 29.0 79 25.0 80 16.0 81 12.5 82 11.5 83 5.5 84 4.5 85 3.0 86 0.5 87 1.5 88 2.0 89 1.5 90 0.5 91 0.0 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2951875634339232 2 0.2544893631987781 3 0.25386130455317396 4 0.2536100810949323 5 0.25574548048998624 6 0.2261011124174731 7 0.2261011124174731 8 0.22760845316692294 9 0.23665249766362187 10-11 0.24676424185784773 12-13 0.23985559675620272 14-15 0.2288017645935707 16-17 0.22302362505401302 18-19 0.21115331665209572 20-21 0.218564408670224 22-23 0.21548692130676392 24-25 0.21661742686885133 26-27 0.21310029845346837 28-29 0.22654075346939598 30-31 0.23740616803834677 32-33 0.23012068774933928 34-35 0.22798528835428536 36-37 0.21894124385758643 38-39 0.2091435289861626 40-41 0.20512395365429645 42-43 0.21755951483725744 44-45 0.21366555123451206 46-47 0.22427974234522124 48-49 0.22742003557324167 50-51 0.23671530352818224 52-53 0.25109784651251593 54-55 0.22811090008340618 56-57 0.20820144101775648 58-59 0.1968963853968828 60-61 0.19953423170842002 62-63 0.20675690613286704 64-65 0.1974616381779265 66-67 0.20173243696803433 68-69 0.2029257483946821 70-71 0.20411905982132988 72-73 0.20738496477847118 74-75 0.20977158763176668 76-77 0.21818757348286152 78-79 0.21894124385758643 80-81 0.2146076392029182 82-83 0.20788741169495442 84-85 0.1927511983358958 86-87 0.19639393848039954 88-89 0.20688251786198786 90-91 0.20468431260237357 92-93 0.20939475244440425 94-95 0.20631726508094422 96-97 0.21353993950539124 98-99 0.21580095062956597 100-101 0.20135560178067188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 796104.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.92917584225546 #Duplication Level Percentage of deduplicated Percentage of total 1 84.62752508402095 58.333055576113644 2 9.82910579321877 13.550243231858172 3 2.5019581303973686 5.1737373576036285 4 0.9751784103498553 2.6887297649830533 5 0.5106183527296169 1.759825111179129 6 0.3139096949566478 1.2982521937353328 7 0.20291946627722926 0.9790950100987834 8 0.1488753975853904 0.8209486766999254 9 0.11229440112513614 0.6966324469129751 >10 0.7089726273786384 9.119866750747196 >50 0.04819683796684907 2.262948118470856 >100 0.019533044895832574 2.3702873155429955 >500 1.8255181955756453E-4 0.12344066687665521 >1k 7.302072782302581E-4 0.8229377791777013 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2394 0.3007144795152392 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1732 0.21755951483725744 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1279 0.1606574015455267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.14256931255212887 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 981 0.12322510626752285 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.1402932280204596E-4 0.0 24-25 0.0 0.0 0.0 5.024469164832735E-4 0.0 26-27 0.0 0.0 0.0 6.280586456040919E-4 0.0 28-29 0.0 0.0 0.0 0.0016957583431310482 0.0 30-31 0.0 0.0 0.0 0.0038939636027453704 0.0 32-33 0.0 0.0 0.0 0.007034256830765829 0.0 34-35 0.0 0.0 0.0 0.009923326600544652 0.0 36-37 0.0 0.0 0.0 0.013063619828565113 0.0 38-39 0.0 0.0 0.0 0.01959542974284767 0.0 40-41 0.0 0.0 0.0 0.033538331675258506 0.0 42-43 0.0 0.0 0.0 0.06550651673650679 0.0 44-45 0.0 0.0 0.0 0.09577894345462401 0.0 46-47 0.0 0.0 0.0 0.17290454513480652 0.0 48-49 0.0 0.0 0.0 0.256624762593832 0.0 50-51 0.0 0.0 0.0 0.315725081145177 0.0 52-53 0.0 0.0 0.0 0.40076422175997106 0.0 54-55 0.0 0.0 0.0 0.49057660808135617 0.0 56-57 0.0 0.0 0.0 0.6976475435370253 0.0 58-59 0.0 0.0 0.0 0.9225553445278506 0.0 60-61 0.0 0.0 0.0 1.1752861435189372 0.0 62-63 0.0 0.0 0.0 1.43511400520535 0.0 64-65 0.0 0.0 0.0 1.7306784038266358 0.0 66-67 0.0 0.0 0.0 2.141617175645393 0.0 68-69 0.0 0.0 0.0 2.6905404319033694 0.0 70-71 0.0 0.0 0.0 3.0866570196858705 0.0 72-73 0.0 0.0 0.0 3.4600378844975026 0.0 74-75 0.0 0.0 0.0 3.965687900073357 0.0 76-77 0.0 0.0 0.0 4.545072000643132 0.0 78-79 0.0 0.0 0.0 5.120624943474722 0.0 80-81 0.0 0.0 0.0 5.946522062444103 0.0 82-83 0.0 0.0 0.0 6.8689517952428325 0.0 84-85 0.0 0.0 0.0 7.874787716177785 0.0 86-87 0.0 0.0 0.0 8.709666073779305 0.0 88-89 0.0 0.0 0.0 9.980856772481987 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3725 0.0 28.453154 1 GGTATCA 2690 0.0 27.916224 1 GCACCGT 105 6.9018875E-5 27.143612 6 AGGCGAC 135 1.3397968E-5 24.634966 5 TACATGG 2860 0.0 24.416553 2 GTACATG 3040 0.0 24.07681 1 ATCAACG 4420 0.0 23.859632 3 TCAACGC 4420 0.0 23.753653 4 CAACGCA 4455 0.0 23.461874 5 AACGCAG 4580 0.0 22.920942 6 TATCAAC 4620 0.0 22.826748 2 ACATGGG 3010 0.0 22.726315 3 GACCGTC 110 0.0028740263 21.594229 7 GAGTACT 2445 0.0 20.786589 12-13 AAGGCGA 160 4.9808932E-5 20.784445 4 AGACCGT 160 4.985727E-5 20.781828 6 ACACGGC 115 0.003718965 20.65535 8 AGTACTT 2615 0.0 20.434273 12-13 GTACTTT 2505 0.0 20.284239 14-15 CATGGGG 1530 0.0 20.182842 4 >>END_MODULE