FastQCFastQC Report
Thu 26 May 2016
SRR937215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796104
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.4328580185503402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.3341271994613769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.21303749258890797No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8190.10287600614995027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.039.6816331
GTATCAA38500.037.764011
CCGTTGC600.003949577531.6677329
ATCAACG45900.031.1483293
TCAACGC46250.030.9126154
TATCAAC47500.030.3991172
CAACGCA47700.029.9729255
AACGCAG48900.029.3345246
TACCGCG650.00584204329.2317568
GTGTACG856.394768E-427.9491421
CGCAGAG53450.026.6613278
ACGCAGA53850.026.4632877
GCAGAGT57900.024.2020249
GTCCTAC1601.8537594E-623.756771
AGGACCG2402.2919266E-1023.7493085
GAGTACT33500.023.4672112-13
AGAGTAC47450.022.7747410-11
AGTACTT34050.022.73938612-13
GTACTTT34700.022.44891514-15
TCTATAC2806.548362E-1122.052933
Produced by FastQC (version 0.11.5)