##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937215_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 796104 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2441992503492 33.0 31.0 34.0 31.0 34.0 2 32.445254640097275 34.0 31.0 34.0 31.0 34.0 3 32.51683197170219 34.0 31.0 34.0 31.0 34.0 4 36.13515947665129 37.0 35.0 37.0 35.0 37.0 5 36.06379945333776 37.0 35.0 37.0 35.0 37.0 6 36.09841302141429 37.0 35.0 37.0 35.0 37.0 7 36.040161838151796 37.0 35.0 37.0 35.0 37.0 8 36.00992458271784 37.0 35.0 37.0 35.0 37.0 9 37.72069855194799 39.0 37.0 39.0 35.0 39.0 10-11 37.64716984213118 39.0 37.0 39.0 35.0 39.0 12-13 37.55204784801985 39.0 37.0 39.0 35.0 39.0 14-15 38.802805914805106 40.0 38.0 41.0 35.0 41.0 16-17 38.75788402017827 40.0 38.0 41.0 35.0 41.0 18-19 38.74606646870258 40.0 38.0 41.0 35.0 41.0 20-21 38.64092568307659 40.0 38.0 41.0 34.0 41.0 22-23 38.59121986574618 40.0 38.0 41.0 34.0 41.0 24-25 38.62270947011948 40.0 38.0 41.0 34.0 41.0 26-27 38.46768010209722 40.0 38.0 41.0 34.0 41.0 28-29 38.33082989157195 40.0 38.0 41.0 34.0 41.0 30-31 38.37224407866309 40.0 38.0 41.0 34.0 41.0 32-33 38.26944720790249 40.0 38.0 41.0 34.0 41.0 34-35 38.1527174841478 40.0 38.0 41.0 34.0 41.0 36-37 38.00170831951604 40.0 37.5 41.0 33.5 41.0 38-39 37.872069478359606 40.0 37.0 41.0 33.0 41.0 40-41 37.68819149256881 40.0 37.0 41.0 33.0 41.0 42-43 37.478813697707835 40.0 37.0 41.0 33.0 41.0 44-45 37.17183370514405 40.0 37.0 41.0 32.0 41.0 46-47 36.81804387366475 40.0 36.5 41.0 31.0 41.0 48-49 36.48527704922975 39.5 36.0 41.0 30.0 41.0 50-51 36.25089372745269 39.0 36.0 41.0 30.0 41.0 52-53 36.020189573221586 39.0 35.5 40.5 29.5 41.0 54-55 35.826777029131875 39.0 35.0 40.0 29.0 41.0 56-57 35.539755484208094 39.0 35.0 40.0 28.0 41.0 58-59 35.29722749791485 38.0 35.0 40.0 28.0 41.0 60-61 34.96385459688684 38.0 34.0 40.0 27.0 41.0 62-63 34.610449137298644 38.0 34.0 40.0 26.0 41.0 64-65 34.215888250781305 37.0 34.0 40.0 26.0 41.0 66-67 33.790407409082235 36.5 33.0 39.0 25.5 41.0 68-69 33.357435711917034 36.0 33.0 39.0 24.5 40.0 70-71 32.910537442344214 35.5 32.0 39.0 23.0 40.0 72-73 32.435550505964045 35.0 32.0 38.0 22.0 40.0 74-75 31.97808891803081 35.0 31.0 37.0 20.5 39.0 76-77 30.62956598635354 34.0 29.5 36.0 20.0 38.5 78-79 31.278199833187625 34.0 31.0 36.0 20.0 39.0 80-81 31.087617321354998 34.0 31.0 36.0 19.5 37.5 82-83 30.697619908956618 34.0 31.0 35.5 18.0 37.0 84-85 30.27799697024509 34.0 30.5 35.0 15.0 37.0 86-87 29.93440492699446 34.0 30.0 35.0 9.0 36.0 88-89 29.5102009285219 34.0 30.0 35.0 6.0 36.0 90-91 28.98821887592576 34.0 29.0 35.0 2.0 35.5 92-93 28.526308748605707 34.0 29.0 35.0 2.0 35.0 94-95 28.07587136856491 33.5 29.0 35.0 2.0 35.0 96-97 27.43791137841287 33.0 27.0 35.0 2.0 35.0 98-99 26.513097534995428 33.0 25.5 34.5 2.0 35.0 100-101 23.57682790188217 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13508351853150913 1101 2 0.16405659476686907 1101 3 0.129705927921961 1101 4 0.05082951798436852 1101 5 0.037268875152633996 1101 6 0.10717734183643302 1101 7 0.08889485140404219 1101 8 0.04931575367474039 1101 9 0.11719682503408535 1101 10-11 0.10505686081988586 1101 12-13 0.2078199331166033 1101 14-15 0.1952908084673055 1101 16-17 0.10702999650517597 1101 18-19 0.09677855093539733 1101 20-21 0.1047152657349315 1101 22-23 0.08879586626109415 1101 24-25 0.10290137398556709 1101 26-27 -0.052416209229591004 1101 28-29 -0.11025406873179833 1101 30-31 0.15830820401423296 1101 32-33 0.19697558993180309 1101 34-35 0.1042046282950011 1101 36-37 0.2625628425728763 1101 38-39 0.2158189309637919 1101 40-41 0.2424589637693657 1101 42-43 0.33918800748661226 1101 44-45 0.3193670354871543 1101 46-47 0.31521130421445065 1101 48-49 0.6337654351379527 1101 50-51 0.556548285340952 1101 52-53 0.5519496097986831 1101 54-55 0.4146746199010565 1101 56-57 0.5639798542614969 1101 58-59 0.581377460933048 1101 60-61 0.48258831961904747 1101 62-63 0.4640470432156789 1101 64-65 0.6066780184676546 1101 66-67 0.5012150433387248 1101 68-69 0.6695044886860089 1101 70-71 0.7420806531997286 1101 72-73 0.45597929396595305 1101 74-75 0.5225118482586062 1101 76-77 0.5371147390277287 1101 78-79 0.624804100354087 1101 80-81 0.634836011245298 1101 82-83 0.7959955652327686 1101 84-85 0.6719723811352374 1101 86-87 0.7342183266321989 1101 88-89 0.9567857945950671 1101 90-91 0.8418664003959009 1101 92-93 0.8989255070043427 1101 94-95 0.8819920184128272 1101 96-97 1.2249154825436293 1101 98-99 1.4869078818367107 1101 100-101 2.1223796692860937 1102 1 0.034605684104569434 1102 2 0.1056281899107887 1102 3 0.18537249551609847 1102 4 0.10514260241702544 1102 5 0.0693135035163337 1102 6 -0.050037043494732814 1102 7 0.017946727304270382 1102 8 -0.036580128718547655 1102 9 -0.04147164018002769 1102 10-11 -0.010759687340794244 1102 12-13 0.14593008652661155 1102 14-15 0.18030875583693273 1102 16-17 0.07008356425934892 1102 18-19 -0.001675917616694278 1102 20-21 0.1378573128782179 1102 22-23 0.04695448900051957 1102 24-25 -0.11272998475605078 1102 26-27 -0.03296903393980699 1102 28-29 -0.08642864976931719 1102 30-31 0.05613851563544614 1102 32-33 -0.06403860792365634 1102 34-35 -0.08323678554619818 1102 36-37 -0.0713954097877263 1102 38-39 0.08455164090229772 1102 40-41 -0.1437432813665538 1102 42-43 -0.1234197338883618 1102 44-45 -0.104472101210078 1102 46-47 -0.07086748130875975 1102 48-49 -0.06171685755277423 1102 50-51 0.10190597527442691 1102 52-53 0.16801536347389145 1102 54-55 0.03017566016260531 1102 56-57 0.12687795435523697 1102 58-59 0.2579132756521787 1102 60-61 0.21838703472456444 1102 62-63 0.04170771218387159 1102 64-65 0.16720295045055167 1102 66-67 0.020570767063546214 1102 68-69 -0.01073968699244432 1102 70-71 -0.18685462195517033 1102 72-73 0.008477064834060855 1102 74-75 -0.030998748691487776 1102 76-77 -0.17359881180790993 1102 78-79 -0.07373724718472374 1102 80-81 0.21480125965061347 1102 82-83 0.20652849922760552 1102 84-85 0.24736505129230935 1102 86-87 0.1800309881013682 1102 88-89 0.20268304876182697 1102 90-91 0.5223506364323924 1102 92-93 0.5078420345884247 1102 94-95 0.557361825678651 1102 96-97 0.4127592832706668 1102 98-99 0.47871153532673105 1102 100-101 1.15586923237624 1103 1 0.06758997729828309 1103 2 0.07499991765948266 1103 3 -0.01174792333207364 1103 4 0.03498553435419183 1103 5 -0.09979644412764799 1103 6 -0.057890446636093884 1103 7 -0.029435471648611156 1103 8 -0.03710368892797078 1103 9 -0.02576483389730555 1103 10-11 0.0075649199890506225 1103 12-13 -0.0015830024786325225 1103 14-15 -0.017597003325377614 1103 16-17 0.09364377368343924 1103 18-19 -0.02196387573188474 1103 20-21 0.09819762701433632 1103 22-23 0.16174506491674379 1103 24-25 -0.06809647690264597 1103 26-27 -0.13349259414922443 1103 28-29 -0.09035535134000128 1103 30-31 -0.1292017985006808 1103 32-33 -0.24165640897077623 1103 34-35 -0.09122107873991325 1103 36-37 0.06865694623321872 1103 38-39 -0.010867207265242484 1103 40-41 -0.06154432848697411 1103 42-43 -0.0807495768203026 1103 44-45 0.03976873648626622 1103 46-47 -0.2736161724082393 1103 48-49 -0.5106697371339308 1103 50-51 -0.5987484749873531 1103 52-53 -0.23512599778265297 1103 54-55 -0.5064735544970702 1103 56-57 -0.44563513464999716 1103 58-59 -0.5096259913635279 1103 60-61 -0.3227124417152325 1103 62-63 -0.47007239252817357 1103 64-65 -0.4354148505965654 1103 66-67 -0.26293708633959767 1103 68-69 -0.28639413725422713 1103 70-71 -0.26146195179810405 1103 72-73 -0.23563288280991657 1103 74-75 -0.0046898481679313875 1103 76-77 -0.21731608929481894 1103 78-79 -0.16169536236796844 1103 80-81 0.0849583277134407 1103 82-83 0.14880598613859775 1103 84-85 0.3433074596692691 1103 86-87 0.15319852736838158 1103 88-89 0.27820660897125293 1103 90-91 0.49761241653710186 1103 92-93 0.3759048618135523 1103 94-95 0.3013408832702744 1103 96-97 0.6999320581397797 1103 98-99 0.9304131060073608 1103 100-101 1.546052478449539 1104 1 0.052678635422147124 1104 2 0.10096098329252357 1104 3 0.17276366152228917 1104 4 0.04141433305831299 1104 5 0.05019198548098558 1104 6 2.652329797641073E-4 1104 7 0.07626728499713664 1104 8 0.038877624889970264 1104 9 0.005511380270640132 1104 10-11 0.0837125581829099 1104 12-13 -0.002060281122574281 1104 14-15 0.05804081449880982 1104 16-17 0.08064516213039497 1104 18-19 -0.11740152578539664 1104 20-21 0.21768361237779033 1104 22-23 0.16716868587072753 1104 24-25 -0.002900937288522698 1104 26-27 0.08860468391620202 1104 28-29 -0.27179355098710545 1104 30-31 0.055823222470657186 1104 32-33 0.07097556580576736 1104 34-35 0.0876427088277012 1104 36-37 0.26325646606197495 1104 38-39 0.2111288298757117 1104 40-41 0.007310347397435635 1104 42-43 0.35729097740144766 1104 44-45 0.3679484246642204 1104 46-47 0.4850833156019405 1104 48-49 0.6455883982974981 1104 50-51 0.6852178551737609 1104 52-53 0.6282204486608975 1104 54-55 0.5965463176501018 1104 56-57 0.5086003517403341 1104 58-59 0.5566144992210624 1104 60-61 0.6127500846797247 1104 62-63 0.5419029625382166 1104 64-65 0.6417307338528246 1104 66-67 0.4879849292225842 1104 68-69 0.6302592716723368 1104 70-71 0.5247967621528957 1104 72-73 0.6819606204153317 1104 74-75 0.6124892904164376 1104 76-77 0.35096006702116966 1104 78-79 0.3502872241930213 1104 80-81 0.6007012914691892 1104 82-83 0.7141136139191069 1104 84-85 0.6739060827926977 1104 86-87 0.6742024034494918 1104 88-89 1.0785197326876599 1104 90-91 1.1279058705881866 1104 92-93 1.1284539636619222 1104 94-95 1.147780095326624 1104 96-97 0.9806514472231171 1104 98-99 0.9552207482508592 1104 100-101 1.646584716452491 1105 1 0.018589916419188057 1105 2 0.07312788551531924 1105 3 0.025045879176147423 1105 4 0.09565916153061949 1105 5 -0.068656787485736 1105 6 5.382639446906978E-4 1105 7 -0.02492280879702946 1105 8 -0.02079570159082067 1105 9 0.1436008059419791 1105 10-11 -0.06765500573845884 1105 12-13 0.02493364795360975 1105 14-15 -0.08954848268736271 1105 16-17 -0.0489883339508026 1105 18-19 0.08481805535298292 1105 20-21 3.268733311543315E-4 1105 22-23 0.07883915355671434 1105 24-25 0.06305611654675403 1105 26-27 0.030412951936249044 1105 28-29 0.15225164366791688 1105 30-31 -0.0672535152911351 1105 32-33 0.12063508013186208 1105 34-35 -0.08476028487653053 1105 36-37 -0.259553664992886 1105 38-39 -0.23728036323748114 1105 40-41 -0.086447682925062 1105 42-43 -0.10114407335019138 1105 44-45 -0.005080131162586099 1105 46-47 -0.08718155655532911 1105 48-49 0.03693010458042778 1105 50-51 0.18965862351501528 1105 52-53 -0.11329551562022289 1105 54-55 -0.0750576589655978 1105 56-57 0.06918511982461695 1105 58-59 0.014531594171593554 1105 60-61 0.012712342163986534 1105 62-63 0.04892949472374397 1105 64-65 -0.11794178959571155 1105 66-67 -0.1248290563870782 1105 68-69 -0.23145455975513585 1105 70-71 -0.1222504882646831 1105 72-73 -0.23113924467525493 1105 74-75 -0.03289969840535534 1105 76-77 -0.12055637907931072 1105 78-79 0.08493726273856694 1105 80-81 -0.20449513021910803 1105 82-83 -0.1267213790142101 1105 84-85 -0.02017878165545639 1105 86-87 0.24163302146311239 1105 88-89 0.4082367441228385 1105 90-91 0.24976601449156632 1105 92-93 0.347207978823171 1105 94-95 0.6396574241201485 1105 96-97 0.6120797203825674 1105 98-99 0.8361912930279374 1105 100-101 1.4835410636193913 1106 1 0.017573760831623986 1106 2 0.013954187223490067 1106 3 0.07049504567130782 1106 4 0.026367640641552725 1106 5 -0.1174631262385688 1106 6 -0.1914816990106516 1106 7 -0.035354481980810704 1106 8 -0.052852194696399124 1106 9 -0.03544838113355553 1106 10-11 -0.21362768530214993 1106 12-13 -0.3029000112355149 1106 14-15 -0.25998221390777587 1106 16-17 -0.1707494987995375 1106 18-19 0.011320422095110416 1106 20-21 0.1058552742366885 1106 22-23 0.01733858759664031 1106 24-25 0.02210761891350188 1106 26-27 0.11192277906117454 1106 28-29 0.17951677023716428 1106 30-31 -0.10337591701988202 1106 32-33 0.02691723695425452 1106 34-35 -0.6523282000854138 1106 36-37 -0.13058551310977862 1106 38-39 -0.004991054737793377 1106 40-41 -0.3019511301364233 1106 42-43 -0.20220396221620973 1106 44-45 -0.3029384867913194 1106 46-47 -0.21514770185270748 1106 48-49 -0.2216853512746937 1106 50-51 -0.2484948031159746 1106 52-53 -0.2588205722162371 1106 54-55 -0.5131686385910328 1106 56-57 -0.4087797391998791 1106 58-59 -0.29795940191813486 1106 60-61 -0.3549160278710062 1106 62-63 -0.25541039002619925 1106 64-65 -0.2644932919624594 1106 66-67 -0.14721406359020506 1106 68-69 -0.08324119280720055 1106 70-71 -0.008010161036857255 1106 72-73 0.12992913359187952 1106 74-75 -0.2785083627395828 1106 76-77 -0.4565437221540165 1106 78-79 -0.3372084977059693 1106 80-81 -0.38074152865499755 1106 82-83 -0.4483063244762384 1106 84-85 -0.10944354806830248 1106 86-87 -0.08659552350498956 1106 88-89 -0.21044765595948078 1106 90-91 -0.2939955619644934 1106 92-93 -0.2676896338543351 1106 94-95 -0.5751823062770534 1106 96-97 0.10453058475794919 1106 98-99 -0.19725566107784687 1106 100-101 0.2076136608616217 1107 1 0.03885138547497746 1107 2 0.07641610511119978 1107 3 0.07413883889148565 1107 4 1.8165485887067234E-4 1107 5 -0.053248222229669295 1107 6 0.00916388346432484 1107 7 0.11895665492135521 1107 8 0.15061267304382397 1107 9 -0.035170321266058124 1107 10-11 0.002243488352284828 1107 12-13 -0.052097264487606765 1107 14-15 -9.174622273349087E-4 1107 16-17 -0.1987452916328465 1107 18-19 -0.09269814746821226 1107 20-21 0.1484794340123088 1107 22-23 0.04923326335465106 1107 24-25 -0.0375483122243665 1107 26-27 -0.11144513041220705 1107 28-29 0.14927715516873974 1107 30-31 0.12608029459576642 1107 32-33 0.06910375467754903 1107 34-35 0.10057933896386118 1107 36-37 0.025913834054009044 1107 38-39 0.10768484835229941 1107 40-41 0.09512319647511447 1107 42-43 0.0019185775584347198 1107 44-45 -0.13716886543631546 1107 46-47 -0.2823064135892537 1107 48-49 -0.07814963871605585 1107 50-51 -0.11845708369047259 1107 52-53 0.01890441448524882 1107 54-55 -0.15077804910411885 1107 56-57 -0.11258432356082437 1107 58-59 0.18622987222471465 1107 60-61 0.10996501086395227 1107 62-63 0.04172559333856185 1107 64-65 0.15376919655246013 1107 66-67 0.08547006040031846 1107 68-69 -0.10862684975386827 1107 70-71 -0.08891953769917649 1107 72-73 -0.13840589227978484 1107 74-75 0.08365736094144793 1107 76-77 -0.07271834375080033 1107 78-79 0.09150178809121812 1107 80-81 -0.039333578560700744 1107 82-83 6.177047321394014E-4 1107 84-85 -0.08800029646038965 1107 86-87 0.10069230472655022 1107 88-89 0.1814051899228133 1107 90-91 0.026254450125065887 1107 92-93 -0.1425699472157156 1107 94-95 0.051419560350925764 1107 96-97 0.406752645786046 1107 98-99 0.8982663660994845 1107 100-101 1.3973206099403974 1108 1 -0.031906369724588046 1108 2 0.04236698711581255 1108 3 0.025493531488464782 1108 4 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58-59 -0.1340375865410195 2316 60-61 0.13715894980213506 2316 62-63 0.07383881838494233 2316 64-65 0.09302071307123327 2316 66-67 0.3000762668473982 2316 68-69 0.48548191346866787 2316 70-71 0.3892507385328443 2316 72-73 0.3403283785378832 2316 74-75 0.4353769177670337 2316 76-77 0.2609909859729669 2316 78-79 0.3113280777588372 2316 80-81 0.4182446286885124 2316 82-83 0.6941461801921065 2316 84-85 0.430229261869183 2316 86-87 0.39756965470398953 2316 88-89 0.670864757777629 2316 90-91 0.5624660358271747 2316 92-93 0.6325184119360365 2316 94-95 1.026892062481572 2316 96-97 1.206869831327733 2316 98-99 1.5024956988312255 2316 100-101 2.311969429311734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 14.0 6 28.0 7 66.0 8 174.0 9 350.0 10 627.0 11 965.0 12 1634.0 13 2259.0 14 3045.0 15 4433.0 16 5980.0 17 8403.0 18 7329.0 19 4517.0 20 3718.0 21 3716.0 22 4031.0 23 4281.0 24 5054.0 25 5920.0 26 7223.0 27 9093.0 28 11023.0 29 13776.0 30 17398.0 31 21659.0 32 27936.0 33 37191.0 34 51714.0 35 75213.0 36 118017.0 37 182091.0 38 145469.0 39 11749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12826541241991 19.84071434764634 15.730149295742791 22.30087094419096 2 15.503125219820527 20.799041331283348 40.45627204485846 23.241561404037665 3 19.054294413795184 24.728804276828154 30.77462241114226 25.4422788982344 4 12.608151698773023 17.583129842332156 38.510169525589625 31.2985489333052 5 13.034324919796306 37.8268755856691 35.07168715409409 14.067112340440499 6 31.15447228000362 37.33544863485172 17.76124727422548 13.748831810919176 7 27.87160973943103 33.289118004682805 21.216700330610074 17.622571925276095 8 26.189166239586786 34.83238370866118 20.32774612362204 18.650703928129992 9 26.01180247806819 16.87304673761217 21.069357772351353 36.045793011968286 10-11 24.279679539356668 27.061037251414387 28.15134706018309 20.507936149045854 12-13 25.429802126462192 24.38516249949753 28.591671021425412 21.593364352614866 14-15 22.391609638941645 25.77326580446776 27.301583712680756 24.53354084390984 16-17 21.341809612814405 29.03703536221398 27.917081185372766 21.70407383959885 18-19 21.166204415503504 27.41281289881724 30.684307577904395 20.736675107774865 20-21 21.984494449203744 26.333560272427402 30.858357346169207 20.82358793219964 22-23 21.60113151685185 26.299287965888684 30.858454251007565 21.241126266251893 24-25 21.5164234298681 26.410008246415195 30.97329681379368 21.10027150992302 26-27 21.362112432435147 26.817671937866088 30.85799667624267 20.96221895345609 28-29 21.14809678497225 27.184407479706664 30.74337310350545 20.924122631815635 30-31 21.452164077068062 26.692764678875747 30.591702188728448 21.263369055327743 32-33 21.23736734574674 26.65137548415623 30.63635729866104 21.474899871435994 34-35 21.669970255142545 26.73790107825108 30.577349190558017 21.014779476048357 36-37 21.718267964989497 26.59162621968989 30.58362977701406 21.106476038306553 38-39 21.285562295331133 26.97910948708898 30.512823089474605 21.222505128105283 40-41 21.591714147900273 26.984225679057005 30.361987880980372 21.062072292062346 42-43 21.470179956659653 26.792374611350144 30.16004522471028 21.577400207279922 44-45 21.64145639263212 27.050988313084723 30.053799503582447 21.253755790700712 46-47 21.771024891220243 27.27325826776401 29.663084220152143 21.292632620863607 48-49 21.691701084280446 27.493392823048246 29.30169927547155 21.513206817199762 50-51 21.762357681910906 27.548913207319647 29.45180529177092 21.236923818998523 52-53 21.920440043009457 27.52642870780702 28.97347582727885 21.579655421904675 54-55 21.68887482037523 27.425499683458444 29.20855817832846 21.67706731783787 56-57 21.89512927959161 27.44993116477244 28.954382844452482 21.700556711183463 58-59 21.836028961040267 27.250710962386826 29.23788851707817 21.67537155949474 60-61 21.72140825821752 27.301772130274436 29.45557364364455 21.521245967863496 62-63 21.32585692321606 27.46682594233919 29.718227769236183 21.489089365208567 64-65 21.364859365108078 27.32544994121371 29.434973320068735 21.874717373609478 66-67 21.500708450152242 27.25221830313627 29.16258428547024 22.084488961241245 68-69 21.67706731783787 26.943653090550985 29.35791052425311 22.021369067358034 70-71 21.66029815200024 26.95075015324631 29.38253042316079 22.006421271592654 72-73 21.347300537744488 26.89074152465196 29.58517930468796 22.17677863291559 74-75 21.293009456050967 27.104875744877553 29.11485182840433 22.48726297066715 76-77 21.120670163697206 27.03359108860149 29.404261252298696 22.441477495402612 78-79 21.358641584516597 27.057582928863567 29.07176700531589 22.51200848130395 80-81 21.196790871544422 27.074603318159436 28.919462783756895 22.809143026539246 82-83 21.091181326980703 26.785625684194397 29.437740586772648 22.685452402052253 84-85 20.87536631658546 26.71658495592896 29.611066937486573 22.79698178999901 86-87 20.61778360616201 26.790029945836224 29.424233517228902 23.167952930772863 88-89 20.5643358154211 26.538304040678103 29.192165847678194 23.705194296222604 90-91 20.695771790370245 26.50616031582022 29.46732033328204 23.3307475605275 92-93 20.06784391753717 26.40894519728876 29.7314255136341 23.791785371539973 94-95 19.91636771075136 26.550048737350902 29.497213931848098 24.03636962004964 96-97 19.99807814054445 26.589804849617636 29.395154401937436 24.016962607900474 98-99 19.912373147695714 26.757341700759824 29.12531418673212 24.204970964812343 100-101 19.676585758667954 25.932623708082968 29.252443759916684 25.138346773332394 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34.0 1 28.5 2 35.0 3 53.0 4 53.0 5 49.5 6 97.5 7 232.5 8 400.5 9 492.5 10 483.0 11 474.5 12 514.5 13 589.0 14 675.0 15 727.5 16 803.5 17 898.0 18 1009.0 19 1171.5 20 1303.5 21 1492.0 22 1788.5 23 2092.5 24 2550.0 25 3280.5 26 4232.5 27 5548.5 28 6938.0 29 8460.5 30 10277.5 31 12227.0 32 14695.5 33 16802.0 34 18963.5 35 21328.0 36 23912.0 37 26298.5 38 27311.5 39 27709.5 40 28299.0 41 29043.5 42 29485.0 43 30071.0 44 30775.5 45 31370.5 46 31669.5 47 32697.5 48 33410.5 49 31923.0 50 29399.0 51 27222.5 52 25432.0 53 24060.0 54 23071.0 55 20963.0 56 17744.0 57 14995.5 58 13353.0 59 11260.5 60 8559.5 61 6716.5 62 5400.0 63 3959.0 64 2683.5 65 1842.0 66 1270.5 67 907.5 68 669.0 69 527.0 70 357.5 71 215.0 72 165.5 73 152.5 74 123.0 75 98.0 76 80.5 77 49.0 78 30.5 79 12.5 80 6.0 81 8.0 82 6.5 83 2.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03780913046536633 2 0.0 3 0.0 4 0.0 5 7.536703747249103E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005024469164832735 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.5122345824163676E-4 22-23 5.652527810436827E-4 24-25 6.280586456040919E-5 26-27 0.002135399395053913 28-29 0.0037683518736245517 30-31 3.1402932280204596E-4 32-33 3.1402932280204596E-4 34-35 0.0 36-37 0.0 38-39 1.2561172912081838E-4 40-41 0.0 42-43 0.009923326600544654 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.2561172912081838E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.3964105192286434E-4 84-85 3.7683518736245515E-4 86-87 0.0 88-89 0.0 90-91 0.010237355923346698 92-93 0.0015073407494498206 94-95 0.0 96-97 0.0 98-99 1.2561172912081838E-4 100-101 0.012184337724719383 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 796104.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.36912152566352 #Duplication Level Percentage of deduplicated Percentage of total 1 82.76889810954503 54.10530159068112 2 10.622221513590677 13.887305779888528 3 2.9427140722763996 5.770879014177484 4 1.2138978799467772 3.1740575213594475 5 0.6241633137906882 2.040050375552218 6 0.39201919756609416 1.5375570339654665 7 0.25538121090470267 1.1685831787700434 8 0.19060788977102835 0.9967896248154106 9 0.12577777176330135 0.7399784202860175 >10 0.7792039969871268 9.639791861649389 >50 0.058614113323946004 2.6514623347809696 >100 0.02495343094714735 2.8545717503584713 >500 9.671872419080965E-4 0.4471214034660458 >1k 5.803123451448578E-4 0.9865501102494597 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3446 0.4328580185503402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2660 0.3341271994613769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.21303749258890797 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 819 0.10287600614995027 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.280586456040919E-5 0.0 18-19 0.0 0.0 0.0 2.5122345824163676E-4 0.0 20-21 0.0 0.0 0.0 2.5122345824163676E-4 0.0 22-23 0.0 0.0 0.0 5.652527810436827E-4 0.0 24-25 0.0 0.0 0.0 7.536703747249103E-4 0.0 26-27 0.0 0.0 0.0 8.792821038457287E-4 0.0 28-29 0.0 0.0 0.0 0.001946981801372685 0.0 30-31 0.0 0.0 0.0 0.004207992925547416 0.0 32-33 0.0 0.0 0.0 0.007285480289007466 0.0 34-35 0.0 0.0 0.0 0.01004893832966547 0.0 36-37 0.0 0.0 0.0 0.013377649151367159 0.0 38-39 0.0 0.0 0.0 0.019846653201089304 0.0 40-41 0.0 0.0 0.0 0.0341035844563022 0.0 42-43 0.0 0.0 0.0 0.0662601871112317 0.0 44-45 0.0 0.0 0.0 0.09678383728759057 0.0 46-47 0.0 0.0 0.0 0.17403505069689387 0.0 48-49 0.0 0.0 0.0 0.2576924622913589 0.0 50-51 0.0 0.0 0.0 0.3176720629465497 0.0 52-53 0.0 0.0 0.0 0.4031508446132666 0.0 54-55 0.0 0.0 0.0 0.4936540954448163 0.0 56-57 0.0 0.0 0.0 0.7029232361600997 0.0 58-59 0.0 0.0 0.0 0.9299036306814186 0.0 60-61 0.0 0.0 0.0 1.18338810004723 0.0 62-63 0.0 0.0 0.0 1.44277632068172 0.0 64-65 0.0 0.0 0.0 1.741166983208224 0.0 66-67 0.0 0.0 0.0 2.154806407203079 0.0 68-69 0.0 0.0 0.0 2.708377297438526 0.0 70-71 0.0 0.0 0.0 3.106754896345201 0.0 72-73 0.0 0.0 0.0 3.4834016661139753 0.0 74-75 0.0 0.0 0.0 3.9929456452925747 0.0 76-77 0.0 0.0 0.0 4.578861555776632 0.0 78-79 0.0 0.0 0.0 5.161009114387065 0.0 80-81 0.0 0.0 0.0 5.9953850250721015 0.0 82-83 0.0 0.0 0.0 6.933139388823571 0.0 84-85 0.0 0.0 0.0 7.9569377870228015 0.0 86-87 0.0 0.0 0.0 8.806889552118818 0.0 88-89 0.0 0.0 0.0 10.106342889873684 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2275 0.0 39.681633 1 GTATCAA 3850 0.0 37.76401 1 CCGTTGC 60 0.0039495775 31.667732 9 ATCAACG 4590 0.0 31.148329 3 TCAACGC 4625 0.0 30.912615 4 TATCAAC 4750 0.0 30.399117 2 CAACGCA 4770 0.0 29.972925 5 AACGCAG 4890 0.0 29.334524 6 TACCGCG 65 0.005842043 29.231756 8 GTGTACG 85 6.394768E-4 27.949142 1 CGCAGAG 5345 0.0 26.661327 8 ACGCAGA 5385 0.0 26.463287 7 GCAGAGT 5790 0.0 24.202024 9 GTCCTAC 160 1.8537594E-6 23.75677 1 AGGACCG 240 2.2919266E-10 23.749308 5 GAGTACT 3350 0.0 23.46721 12-13 AGAGTAC 4745 0.0 22.77474 10-11 AGTACTT 3405 0.0 22.739386 12-13 GTACTTT 3470 0.0 22.448915 14-15 TCTATAC 280 6.548362E-11 22.05293 3 >>END_MODULE