FastQCFastQC Report
Thu 26 May 2016
SRR937214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739561
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.31680956675649474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.22851394273089035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.1490073165026279No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10110.1367027195863492No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9670.13075324415430234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34900.031.7549231
GCGGTAT650.005804267729.2703021
GGTATCA22400.028.0289291
AATCGAT350.00839392127.14180270-71
AACGCGA350.00839808427.13904476-77
TCAACGC41000.026.669714
ATCAACG40950.026.582573
TATCAAC41400.026.4102382
CAACGCA41600.026.397545
AACGCAG42200.026.1153836
CCTATAC1858.885763E-925.6947063
GTACATG29800.022.664841
CGCAGAG47700.022.6062418
ACGCAGA48650.022.5553727
TACATGG29100.022.3806362
GTATAGG2405.828042E-921.8002781
GCAGAGT49100.021.382639
AGAGTAC36700.021.09805510-11
ACATGGG30000.021.073943
GGACCGA1150.003720678420.6534716
Produced by FastQC (version 0.11.5)