##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739561 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.808635122728212 33.0 31.0 34.0 30.0 34.0 2 31.925551780042486 33.0 31.0 34.0 30.0 34.0 3 31.97383853394108 33.0 31.0 34.0 30.0 34.0 4 35.576298642032235 37.0 35.0 37.0 33.0 37.0 5 35.59001759151713 37.0 35.0 37.0 33.0 37.0 6 35.62541697033781 37.0 35.0 37.0 33.0 37.0 7 35.577831984109494 37.0 35.0 37.0 33.0 37.0 8 35.56885638912814 37.0 35.0 37.0 33.0 37.0 9 37.204575417037944 39.0 37.0 39.0 34.0 39.0 10-11 37.09240819891801 39.0 37.0 39.0 33.0 39.0 12-13 36.968880187029875 39.0 37.0 39.0 33.0 39.0 14-15 38.18245283350528 40.0 38.0 41.0 33.0 41.0 16-17 38.15631300190248 40.0 38.0 41.0 33.0 41.0 18-19 38.13227103646622 40.0 38.0 41.0 33.5 41.0 20-21 38.06900647816745 40.0 38.0 41.0 33.0 41.0 22-23 37.928391302407775 40.0 38.0 41.0 33.0 41.0 24-25 37.78038525557729 40.0 38.0 41.0 33.0 41.0 26-27 37.62928818582916 40.0 37.0 41.0 32.5 41.0 28-29 37.69606293463284 40.0 37.0 41.0 33.0 41.0 30-31 37.5549616596873 40.0 37.0 41.0 32.5 41.0 32-33 37.367480167288434 40.0 37.0 41.0 32.0 41.0 34-35 37.27201948182773 40.0 37.0 41.0 32.0 41.0 36-37 37.302311100774645 40.0 37.0 41.0 32.0 41.0 38-39 37.21393637576887 40.0 37.0 41.0 31.0 41.0 40-41 37.059917302291495 40.0 37.0 41.0 31.0 41.0 42-43 36.83529012481729 40.0 36.0 41.0 31.0 41.0 44-45 36.62277824276835 40.0 36.0 41.0 30.0 41.0 46-47 36.31239478555521 39.5 36.0 41.0 30.0 41.0 48-49 36.01390419451539 39.0 36.0 41.0 29.0 41.0 50-51 35.1366290272202 38.5 34.5 40.0 27.0 40.5 52-53 35.20877926229209 38.5 35.0 40.0 27.5 40.5 54-55 35.55080175942214 39.0 35.0 40.0 28.0 41.0 56-57 35.37463305934196 39.0 35.0 40.5 27.0 41.0 58-59 35.158773921285736 39.0 35.0 40.0 26.0 41.0 60-61 34.88481680348207 38.0 34.5 40.0 26.0 41.0 62-63 34.57662991964152 38.0 34.0 40.0 26.0 41.0 64-65 34.22226496529698 37.5 34.0 40.0 25.0 41.0 66-67 33.86104797305428 37.0 34.0 40.0 23.5 41.0 68-69 33.39740670478838 36.5 33.0 39.0 22.5 41.0 70-71 32.977130351654566 36.0 33.0 39.0 22.0 40.5 72-73 32.57307916453139 35.5 32.0 38.5 20.5 40.0 74-75 32.12643717015905 35.0 32.0 37.5 19.5 39.5 76-77 31.691154617401402 35.0 31.5 37.0 18.0 39.0 78-79 31.21860941829004 35.0 31.0 36.5 15.0 39.0 80-81 30.75366737835013 35.0 31.0 36.0 10.0 38.5 82-83 30.282145083367023 34.0 30.5 35.5 7.0 37.0 84-85 29.854618483127155 34.0 30.0 35.0 4.0 37.0 86-87 29.445479818432826 34.0 29.5 35.0 2.0 36.0 88-89 29.04825227398416 34.0 29.0 35.0 2.0 36.0 90-91 28.652011801595812 34.0 29.0 35.0 2.0 35.5 92-93 28.228347627849494 34.0 29.0 35.0 2.0 35.0 94-95 27.7360258315406 34.0 27.5 35.0 2.0 35.0 96-97 27.148957286822856 33.0 26.5 35.0 2.0 35.0 98-99 26.417362462325624 33.0 25.0 35.0 2.0 35.0 100-101 24.525659141030964 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14585790273205745 1101 2 0.0602914739122653 1101 3 -9.444300544565465E-4 1101 4 0.07045214681162548 1101 5 0.20129987524541804 1101 6 0.02574401678872107 1101 7 -0.06523636563449742 1101 8 -0.003359536355993953 1101 9 0.0523517921778307 1101 10-11 -0.05052135410381453 1101 12-13 -0.009715290438940372 1101 14-15 -0.03900969991538261 1101 16-17 0.14773848137625833 1101 18-19 0.037726263821475925 1101 20-21 0.11268031480450702 1101 22-23 0.16608455495945407 1101 24-25 0.15256254558932625 1101 26-27 0.2062015776921342 1101 28-29 0.17428354613628727 1101 30-31 0.058512863718526376 1101 32-33 0.06718583088967733 1101 34-35 0.1402495464914395 1101 36-37 0.1964640817788066 1101 38-39 0.1579443242895806 1101 40-41 0.06524741315259064 1101 42-43 0.183725473389174 1101 44-45 0.2756017225232341 1101 46-47 0.2230179934939045 1101 48-49 0.2997076714312854 1101 50-51 0.14854993380630077 1101 52-53 0.21546556285432672 1101 54-55 0.3767087917902714 1101 56-57 0.34196366037023296 1101 58-59 0.298791040168652 1101 60-61 0.1866162891315568 1101 62-63 0.31486167757790895 1101 64-65 0.29262992216181516 1101 66-67 0.4919885060180391 1101 68-69 0.45154643713856757 1101 70-71 0.4471560322758563 1101 72-73 0.36294528111842084 1101 74-75 0.24168512076630577 1101 76-77 0.5745415441892767 1101 78-79 0.47040380427624484 1101 80-81 0.3733757826809416 1101 82-83 0.3773596803428809 1101 84-85 0.29451817185761087 1101 86-87 0.25048885507039387 1101 88-89 0.5384394135601767 1101 90-91 0.5296162615204025 1101 92-93 0.7152573776313673 1101 94-95 0.9944946906868317 1101 96-97 0.8469057305190262 1101 98-99 0.958554393651216 1101 100-101 1.387962128833955 1102 1 -0.022217629500030256 1102 2 -0.08920421502407905 1102 3 -0.00930738096904804 1102 4 0.051597244443158274 1102 5 0.027636528780000447 1102 6 -0.06429848380868464 1102 7 -0.06766713701997418 1102 8 -0.22359088919181147 1102 9 -0.0032375742261265827 1102 10-11 -0.10932154787246162 1102 12-13 0.0906805491662439 1102 14-15 0.07927977918338058 1102 16-17 0.03125998832456389 1102 18-19 0.049536477392933875 1102 20-21 9.016500033354191E-4 1102 22-23 0.16783634194100472 1102 24-25 0.24808745720584824 1102 26-27 0.010266026704051967 1102 28-29 -0.03957488290821942 1102 30-31 0.12387224175292744 1102 32-33 0.24080549999577983 1102 34-35 0.3327118449890136 1102 36-37 0.15616251307576334 1102 38-39 0.31951734453204494 1102 40-41 0.4010916531918056 1102 42-43 0.32781672080733415 1102 44-45 0.45330721006002506 1102 46-47 0.4833303624167016 1102 48-49 0.4157400056720988 1102 50-51 0.29043890379309545 1102 52-53 0.39862241799782083 1102 54-55 0.5496870173632615 1102 56-57 0.25815691499769144 1102 58-59 0.3326547104962714 1102 60-61 0.5333083969576009 1102 62-63 0.2811355367866497 1102 64-65 0.23411417268359713 1102 66-67 0.42378865574835345 1102 68-69 0.5024193706760585 1102 70-71 0.5022822742830826 1102 72-73 0.32548598950021557 1102 74-75 0.5332206651125446 1102 76-77 0.7650040693759514 1102 78-79 0.6778311933736525 1102 80-81 0.5472744815287989 1102 82-83 0.5436964205102406 1102 84-85 0.6310543912175781 1102 86-87 0.47235070899742126 1102 88-89 0.47534512172177656 1102 90-91 0.8453959890169926 1102 92-93 0.7742965334815679 1102 94-95 0.5550141841449765 1102 96-97 0.6022897772371962 1102 98-99 0.5386200468587603 1102 100-101 0.8538919837168315 1103 1 -0.08229849915596787 1103 2 -0.28533311895455427 1103 3 -0.013157806231891556 1103 4 -0.21000058941231003 1103 5 -0.15687577181252976 1103 6 -0.25332911072890596 1103 7 -0.2094834964034007 1103 8 -0.10984782536874604 1103 9 -0.078865425603027 1103 10-11 -0.21097413986486657 1103 12-13 -0.19525325940062999 1103 14-15 -0.15400392471316593 1103 16-17 -0.16609112580266583 1103 18-19 -0.2671978081625568 1103 20-21 -0.2463519115669044 1103 22-23 -0.6266558631172998 1103 24-25 -0.37090277388792714 1103 26-27 -0.2355683669831663 1103 28-29 -0.36446438672110304 1103 30-31 -0.32455312054683105 1103 32-33 -0.3653293419164214 1103 34-35 -0.17713847691980789 1103 36-37 -0.22774701899373184 1103 38-39 -0.25474155342143945 1103 40-41 -0.03554988674428472 1103 42-43 -0.3220129887970842 1103 44-45 -0.24245166693014397 1103 46-47 -0.06265925732820676 1103 48-49 -6.709572657328522E-4 1103 50-51 -0.013265948288754714 1103 52-53 0.03617298590874185 1103 54-55 -0.050605396548192516 1103 56-57 -0.042425377726281965 1103 58-59 0.039496845010575043 1103 60-61 0.17542750565581144 1103 62-63 -0.027306050146279404 1103 64-65 0.09959451055377855 1103 66-67 0.045489005104485614 1103 68-69 0.18135737873182478 1103 70-71 0.13315139395047737 1103 72-73 0.12221346929010934 1103 74-75 0.24869627404818573 1103 76-77 0.20460826242958774 1103 78-79 0.27453156710573623 1103 80-81 0.04875410177097805 1103 82-83 0.006097978231032641 1103 84-85 0.1567125175259605 1103 86-87 0.27436533261811036 1103 88-89 0.46030905993946547 1103 90-91 0.4025366314305643 1103 92-93 0.40760422331416635 1103 94-95 0.6026350425489753 1103 96-97 0.36958134579066027 1103 98-99 0.31376895954507944 1103 100-101 0.8099923580339379 1104 1 -0.004024548226194469 1104 2 -0.024010038255163124 1104 3 -0.024625186111180142 1104 4 -0.05982979994086435 1104 5 -0.0458158320597164 1104 6 0.006230785886053525 1104 7 -0.145632172557157 1104 8 -0.11828947928645306 1104 9 0.13675947186860782 1104 10-11 -0.020872273757490234 1104 12-13 0.003064850116068385 1104 14-15 -0.004833204505331423 1104 16-17 -0.08421911279567951 1104 18-19 -0.17955322038429955 1104 20-21 -0.3015835618756171 1104 22-23 -0.15923163081931335 1104 24-25 -0.29703982104671667 1104 26-27 -0.26256537649658185 1104 28-29 -0.16987557558837096 1104 30-31 -0.22685194318704305 1104 32-33 -0.17106983670159792 1104 34-35 -0.3462230915285929 1104 36-37 -0.2705816989168852 1104 38-39 -0.23934984342452026 1104 40-41 -0.17743759950203497 1104 42-43 -0.14951683778784286 1104 44-45 -0.2865823053826304 1104 46-47 -0.09165579779334365 1104 48-49 -0.18520439396790778 1104 50-51 -0.30754574652508637 1104 52-53 -0.2957886444836433 1104 54-55 -0.07873188890550153 1104 56-57 -0.17642311511514208 1104 58-59 -0.19364992214097754 1104 60-61 -0.18818880798924198 1104 62-63 -0.22766561332380064 1104 64-65 -0.140543020235107 1104 66-67 0.031819771633884386 1104 68-69 0.03343218828728567 1104 70-71 0.06451298278469864 1104 72-73 0.1966208097742097 1104 74-75 0.08209239018259495 1104 76-77 0.07293328615540773 1104 78-79 0.07088866595705312 1104 80-81 0.03722960979522938 1104 82-83 0.051060995110692176 1104 84-85 0.3626070934564929 1104 86-87 0.5332107204517769 1104 88-89 0.3042460033243657 1104 90-91 0.25530772250248646 1104 92-93 0.4814216079932976 1104 94-95 0.3273924072753296 1104 96-97 0.47678864933416065 1104 98-99 0.6179127459312568 1104 100-101 0.6740570623937785 1105 1 -0.22851617649718392 1105 2 -0.2293833878895093 1105 3 -0.06356955066335246 1105 4 -0.1631412306543112 1105 5 -0.016750840985871207 1105 6 -0.022205708238253408 1105 7 0.052272538326761264 1105 8 0.12342595824468816 1105 9 -0.12609142022295572 1105 10-11 -0.09051572145232711 1105 12-13 -0.06059216413435564 1105 14-15 0.0386542951392741 1105 16-17 0.11384496695758628 1105 18-19 -0.09696273552884094 1105 20-21 -0.47272552586872507 1105 22-23 -0.11978521691815303 1105 24-25 -0.13242674322233228 1105 26-27 0.012323476810735201 1105 28-29 0.07227307591156773 1105 30-31 -0.20277302791291874 1105 32-33 -0.28906564971477877 1105 34-35 -0.36886843518922774 1105 36-37 -0.1673914783294208 1105 38-39 0.1831534898539502 1105 40-41 -0.1478915089775299 1105 42-43 -0.001182579167334552 1105 44-45 -0.18433782306210134 1105 46-47 0.11790753150464894 1105 48-49 0.1799665497202625 1105 50-51 0.20395275480897368 1105 52-53 0.01724249155451929 1105 54-55 0.016003551289273332 1105 56-57 0.08926349535591527 1105 58-59 0.2303939768745238 1105 60-61 0.19378470236075884 1105 62-63 0.03833000814744736 1105 64-65 0.08067294179171114 1105 66-67 0.24877691861687623 1105 68-69 0.28803330970541907 1105 70-71 0.3246067186154491 1105 72-73 0.3679617978013354 1105 74-75 0.06226331142444508 1105 76-77 0.11600253604430222 1105 78-79 -0.0521979723474395 1105 80-81 -0.08476314453279699 1105 82-83 0.11324627900426165 1105 84-85 0.2720668703999358 1105 86-87 0.16889572975203038 1105 88-89 0.4071406807034137 1105 90-91 0.35276066379339 1105 92-93 0.07699205127818232 1105 94-95 0.12810799594502598 1105 96-97 0.3506096563957719 1105 98-99 0.5977469723787046 1105 100-101 1.0165085895665449 1106 1 -0.028506246939755897 1106 2 -0.1246742952792026 1106 3 -0.18072305207354233 1106 4 -0.0672885394578202 1106 5 -0.022233102404385363 1106 6 -0.03716305887956395 1106 7 -0.004553173116477183 1106 8 -0.2601795786292911 1106 9 -0.17667487719837283 1106 10-11 -0.43626863969197416 1106 12-13 -0.5982423725595822 1106 14-15 -0.6455810048192916 1106 16-17 -0.5013627231027442 1106 18-19 -0.24847727946617226 1106 20-21 -0.13318908343820368 1106 22-23 0.006801364048790504 1106 24-25 -0.056739646491259066 1106 26-27 0.10485358532934441 1106 28-29 0.029036536667852886 1106 30-31 -0.06240059312815305 1106 32-33 0.12915203291746735 1106 34-35 -0.06954001698682077 1106 36-37 -0.3226754350289127 1106 38-39 -0.07602896385426305 1106 40-41 0.18684341957739292 1106 42-43 -0.11217498114636726 1106 44-45 -0.1936353259693604 1106 46-47 0.1772280442517129 1106 48-49 0.19378963937842997 1106 50-51 0.0521365050882352 1106 52-53 -0.1723299455216889 1106 54-55 -0.1641930921384116 1106 56-57 -0.1622550585950009 1106 58-59 0.04733208326973681 1106 60-61 0.007944442206358815 1106 62-63 0.08762835124802848 1106 64-65 0.21513442561130347 1106 66-67 0.032523569778156514 1106 68-69 -0.04251210942693007 1106 70-71 0.09911315277771138 1106 72-73 0.15102770141970723 1106 74-75 -0.2375071630365433 1106 76-77 0.061018566550956166 1106 78-79 0.08723401877706394 1106 80-81 0.35307782596687076 1106 82-83 0.42665939251246954 1106 84-85 0.4895442281446982 1106 86-87 0.3154464497404206 1106 88-89 0.4456511325173693 1106 90-91 0.4820473205686895 1106 92-93 0.6742969402485457 1106 94-95 0.6994490017710042 1106 96-97 0.5432211109040779 1106 98-99 0.8680829924211366 1106 100-101 0.9855827920034876 1107 1 -0.2001468348341504 1107 2 -0.3820480300121183 1107 3 -0.13241305237855627 1107 4 -0.04865866630043314 1107 5 -0.10229297246804236 1107 6 -0.1584456900250686 1107 7 -0.2284689541506566 1107 8 -0.012185051467717756 1107 9 -0.07245129257635341 1107 10-11 -0.05118953190407183 1107 12-13 -0.04920796252371673 1107 14-15 0.10000608346508955 1107 16-17 -0.07259894841287462 1107 18-19 -0.11686989462018715 1107 20-21 -0.17919622078640174 1107 22-23 0.02085274598406528 1107 24-25 -0.1622170601393691 1107 26-27 -0.08291466436455863 1107 28-29 -0.05794826051903357 1107 30-31 0.006171109334182745 1107 32-33 -0.10406779130681088 1107 34-35 0.059848384604485716 1107 36-37 -0.11280885725229695 1107 38-39 -0.20494350304214493 1107 40-41 -0.18065593771267885 1107 42-43 -0.2821063963718373 1107 44-45 -0.28580973347795435 1107 46-47 -0.33186248852213396 1107 48-49 -0.24252325360055949 1107 50-51 -0.3193520111015147 1107 52-53 -0.3524194456023011 1107 54-55 -0.24169846257898087 1107 56-57 0.03353290377487639 1107 58-59 -0.05674335126754215 1107 60-61 -0.12455620050356941 1107 62-63 -0.14027876736354727 1107 64-65 -0.1869459937405864 1107 66-67 -0.013227572938312449 1107 68-69 -0.06999939684245504 1107 70-71 0.13609838570015143 1107 72-73 0.015106534694218965 1107 74-75 0.04769373283573941 1107 76-77 0.2980957396730446 1107 78-79 0.2854664575453292 1107 80-81 0.061288608678626844 1107 82-83 0.15039325395728298 1107 84-85 0.319885147633844 1107 86-87 0.19095026896246026 1107 88-89 0.12771887196494447 1107 90-91 0.22108748231372033 1107 92-93 0.31675196144708195 1107 94-95 0.4978123790700515 1107 96-97 0.29513181126181465 1107 98-99 0.3850646958856707 1107 100-101 0.33974428431057646 1108 1 0.18820259800041228 1108 2 0.09807697787979563 1108 3 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-1.1872175379936927 2316 82-83 -1.5677152666022778 2316 84-85 -1.582710019809987 2316 86-87 -1.8407784296386396 2316 88-89 -1.9437283514347996 2316 90-91 -2.422676824595989 2316 92-93 -2.166086529438889 2316 94-95 -2.148681898166064 2316 96-97 -1.9933234663982091 2316 98-99 -2.1418695983067835 2316 100-101 -2.748823752993367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2610.0 3 525.0 4 434.0 5 510.0 6 547.0 7 676.0 8 758.0 9 990.0 10 1278.0 11 1652.0 12 2190.0 13 2816.0 14 3325.0 15 4207.0 16 5375.0 17 7035.0 18 5958.0 19 4183.0 20 4019.0 21 4114.0 22 4423.0 23 5057.0 24 5693.0 25 6443.0 26 7378.0 27 8554.0 28 10542.0 29 12679.0 30 15479.0 31 19460.0 32 25234.0 33 33743.0 34 46377.0 35 66164.0 36 101720.0 37 155442.0 38 142135.0 39 19835.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33999267607926 19.954157681300945 15.149801304742915 22.556048337876877 2 14.722593922730582 21.111685043810947 40.58142545902188 23.584295574436588 3 18.7432553347252 25.12458989723706 30.152924269949295 25.979230498088445 4 12.122929416454038 17.555457338318913 38.46208076529906 31.85953247992798 5 13.006795231948978 38.279038363504235 34.7367593305639 13.977407073982883 6 30.707851649513028 37.7641470063791 17.872027922757155 13.65597342135072 7 27.73338644036568 33.48140982913625 21.08850449513095 17.696699235367117 8 25.774669450432132 35.71059858634934 20.049208157823788 18.465523805394742 9 25.635651773376324 17.605332321781773 20.848619503075298 35.910396401766604 10-11 24.02500084691216 27.79064331447542 27.718283139672756 20.466072698939666 12-13 25.157003047247922 25.0616156511206 28.054259711155037 21.727121590476436 14-15 22.16372941865861 26.689541409946788 26.527092344638604 24.619636826755997 16-17 21.254210236802955 30.03710969783845 27.03602038797857 21.67265967738002 18-19 21.11771495768992 28.796323642975008 29.32164330557821 20.76431809375686 20-21 22.086800077762554 27.65271569455574 29.455546983009423 20.804937244672285 22-23 21.626011854360712 27.681896697713803 29.33608806096528 21.356003386960204 24-25 21.402184779693254 27.971425010634004 29.472648259681005 21.153741949991737 26-27 21.237500101597657 28.227012514122073 29.254774412562345 21.28071297171792 28-29 21.022263106484385 28.71538366703833 29.23663542994634 21.025717796530948 30-31 21.383268160890573 28.2567929738416 29.068399901360625 21.291538963907204 32-33 21.039856820017018 28.18706882068902 29.20408295984782 21.568991399446137 34-35 21.63549350431149 28.35671546336995 29.004184123095598 21.003606909222956 36-37 21.55507237541742 28.147839575162735 29.051296119431292 21.245791929988552 38-39 21.185767913049013 28.585420835235574 28.965011792788314 21.263799458927092 40-41 21.650017340226075 28.415008507548418 28.83483163724247 21.100142514983038 42-43 21.29798909299539 28.52768985144608 28.573683244383417 21.60063781117511 44-45 21.58496818358552 28.436369203173918 28.62562843684337 21.35303417639719 46-47 21.645530433533825 28.723012944573867 28.177293717658042 21.45416290423427 48-49 21.55284426168427 28.71495058023345 28.122116615745224 21.61008854233706 50-51 21.650688611172647 28.86997853107574 28.004650132410898 21.474682725340717 52-53 21.671133973120277 28.97396043655838 27.752678351225146 21.6022272390962 54-55 21.644454771532374 28.86351827476813 27.954606577466823 21.537420376232674 56-57 21.70385065805471 29.002700493160038 27.687539327159293 21.605909521625957 58-59 21.83821860977055 28.675234950469903 27.82910845821692 21.65743798154263 60-61 21.477265569280224 28.83243690637537 28.119494695604292 21.570802828740117 62-63 21.309160124365807 28.727689004192943 28.416706744609797 21.54644412683145 64-65 21.307098347331348 28.702926036304525 28.27038742888106 21.719588187483065 66-67 21.298209063188736 28.68201355428011 27.941106747744904 22.07867063478625 68-69 21.31836813203187 28.489013147379055 28.155723160030664 22.036895560558406 70-71 21.43881890265132 28.327221297250873 28.12868737307986 22.105272427017955 72-73 21.128123846390917 28.340326956097663 28.36356000799271 22.16798918951871 74-75 21.097715779020824 28.745879908040493 27.940150457020312 22.21625385591837 76-77 21.26143615093721 28.454518545411688 28.12641184435388 22.157633459297227 78-79 21.27504072557566 28.427366232147634 27.958031489929525 22.33956155234718 80-81 21.3076810530052 28.31570376913219 27.842157598549893 22.534457579312715 82-83 21.316006935977676 28.197390880273105 28.216559646698634 22.270042537050582 84-85 21.20390958723932 28.20424078191745 28.231603287022523 22.360246343820712 86-87 21.153476250206573 28.168558107277057 28.07509231440267 22.6028733281137 88-89 21.033183091151063 27.967328251512864 28.003291556750572 22.996197100585505 90-91 21.04295301286098 27.853080864045342 28.477842108257633 22.626124014836048 92-93 20.98248755787586 28.02742550303569 28.395812619036654 22.5942743200518 94-95 20.77097815898267 28.276355784808278 28.104935495581408 22.847730560627653 96-97 20.761539550385404 28.488820589647606 27.92519381866281 22.824446041304185 98-99 21.147380427302725 28.723117478447925 27.423084425612153 22.706417668637194 100-101 21.31895743102987 28.33851999092476 27.24116095344199 23.10136162460338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 939.0 1 519.0 2 93.5 3 79.0 4 69.0 5 74.5 6 93.0 7 155.0 8 261.5 9 339.5 10 378.0 11 424.0 12 487.5 13 550.5 14 607.5 15 668.0 16 719.5 17 784.0 18 884.0 19 1012.5 20 1126.5 21 1287.5 22 1545.5 23 1948.0 24 2402.0 25 3039.0 26 4046.0 27 5433.0 28 6828.5 29 8245.0 30 10022.0 31 11901.5 32 14317.5 33 16359.0 34 18190.5 35 20225.0 36 22695.0 37 24933.0 38 25797.0 39 26306.5 40 26591.0 41 27330.0 42 27678.5 43 28196.0 44 28996.0 45 29084.0 46 29102.0 47 29584.0 48 30002.0 49 28942.0 50 26894.0 51 24861.0 52 23025.0 53 21520.5 54 20759.0 55 18905.0 56 16014.5 57 13746.0 58 12092.5 59 10148.0 60 7934.5 61 6179.0 62 4627.5 63 3351.0 64 2371.5 65 1732.5 66 1260.5 67 876.0 68 647.0 69 501.5 70 338.5 71 231.5 72 182.0 73 135.5 74 100.0 75 71.0 76 56.0 77 44.0 78 25.5 79 17.0 80 13.5 81 12.0 82 8.5 83 3.5 84 5.5 85 5.0 86 3.0 87 4.0 88 2.5 89 2.0 90 3.0 91 3.0 92 1.5 93 1.5 94 2.5 95 2.5 96 4.0 97 3.5 98 2.5 99 4.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30436975448948766 2 0.26502208742754146 3 0.26245299576370307 4 0.26596859488263985 5 0.2684024711957499 6 0.20620340986071467 7 0.19322273619079428 8 0.19903699627211277 9 0.19214101338496756 10-11 0.21445154625514326 12-13 0.2052569024056163 14-15 0.20059197280548866 16-17 0.19998350372721113 18-19 0.19457488969807765 20-21 0.19153254430669006 22-23 0.19416924364589264 24-25 0.18429852304272398 26-27 0.1832168002368973 28-29 0.19389881294443595 30-31 0.20539211775634464 32-33 0.19863135021992778 34-35 0.20728513266654136 36-37 0.18876062961675913 38-39 0.18903106031821582 40-41 0.1883549835645741 42-43 0.19085646755304836 44-45 0.19200579803423923 46-47 0.1964002969329102 48-49 0.2022821646895929 50-51 0.20518929473025213 52-53 0.21729106862043834 54-55 0.20018632675330364 56-57 0.18423091536735983 58-59 0.18571828422537154 60-61 0.18287876186007646 62-63 0.19146493663132588 64-65 0.18402809234126732 66-67 0.1817294313788856 68-69 0.17780818620776379 70-71 0.18930149101967247 72-73 0.18774651448629662 74-75 0.19065364452695585 76-77 0.18747608378483993 78-79 0.18774651448629662 80-81 0.19024799847477084 82-83 0.18673239935583408 84-85 0.179092732039683 86-87 0.17888990901359048 88-89 0.1769292864280296 90-91 0.18375766163981064 92-93 0.18159421602815723 94-95 0.17807861690922047 96-97 0.17665885572657294 98-99 0.18240550813252726 100-101 0.17422497941346285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 739561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.51887598515111 #Duplication Level Percentage of deduplicated Percentage of total 1 85.09484081882621 59.15697685860154 2 9.501671067754316 13.210909852218213 3 2.4006176385484554 5.0066471970604915 4 0.9689561916573907 2.694429812914978 5 0.48630727394388673 1.6903767533990988 6 0.3142474361584718 1.3107677125771493 7 0.1981887218819263 0.9644500024715668 8 0.1586283369892825 0.882213094951095 9 0.11285674478683325 0.7061106640431339 >10 0.7000754562969028 9.049881891923246 >50 0.044683703444016995 2.147038816624155 >100 0.017951011275822104 2.2153543626559435 >500 1.9511968729679085E-4 0.13116872799113316 >1k 7.804787491871634E-4 0.8336742525682568 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.31680956675649474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1690 0.22851394273089035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1102 0.1490073165026279 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1011 0.1367027195863492 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 967 0.13075324415430234 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3521535072833748E-4 0.0 12-13 0.0 0.0 0.0 2.7043070145667497E-4 0.0 14-15 0.0 0.0 0.0 2.7043070145667497E-4 0.0 16-17 0.0 0.0 0.0 2.7043070145667497E-4 0.0 18-19 0.0 0.0 0.0 4.0564605218501245E-4 0.0 20-21 0.0 0.0 0.0 4.0564605218501245E-4 0.0 22-23 0.0 0.0 0.0 4.0564605218501245E-4 0.0 24-25 0.0 0.0 0.0 4.0564605218501245E-4 0.0 26-27 0.0 0.0 0.0 6.760767536416874E-4 0.0 28-29 0.0 0.0 0.0 0.001893014910196725 0.0 30-31 0.0 0.0 0.0 0.003718422145029281 0.0 32-33 0.0 0.0 0.0 0.005881867756682681 0.0 34-35 0.0 0.0 0.0 0.008924213148070275 0.0 36-37 0.0 0.0 0.0 0.012439812267007048 6.760767536416874E-5 38-39 0.0 0.0 0.0 0.018592110725146403 1.3521535072833748E-4 40-41 0.0 0.0 0.0 0.034344699084997724 1.3521535072833748E-4 42-43 0.0 0.0 0.0 0.0659850911554287 1.3521535072833748E-4 44-45 0.0 0.0 0.0 0.09140557709235614 1.3521535072833748E-4 46-47 0.0 0.0 0.0 0.15847239105361152 1.3521535072833748E-4 48-49 0.0 0.0 0.0 0.232773226278833 1.3521535072833748E-4 50-51 0.0 0.0 0.0 0.2862508974918905 1.3521535072833748E-4 52-53 0.0 0.0 0.0 0.3700168072680955 1.3521535072833748E-4 54-55 0.0 0.0 0.0 0.45574333962986147 1.3521535072833748E-4 56-57 0.0 0.0 0.0 0.6609326343601136 1.3521535072833748E-4 58-59 0.0 0.0 0.0 0.8692994898324817 1.3521535072833748E-4 60-61 0.0 0.0 0.0 1.108630660621639 1.3521535072833748E-4 62-63 0.0 0.0 0.0 1.3533028377645657 1.3521535072833748E-4 64-65 0.0 0.0 0.0 1.6324549293432185 1.3521535072833748E-4 66-67 0.0 0.0 0.0 2.0387094506065084 1.3521535072833748E-4 68-69 0.0 0.0 0.0 2.5911317660071314 1.3521535072833748E-4 70-71 0.0 0.0 0.0 2.9605401041969492 1.3521535072833748E-4 72-73 0.0 0.0 0.0 3.331097772867958 1.3521535072833748E-4 74-75 0.0 0.0 0.0 3.836667969241212 1.3521535072833748E-4 76-77 0.0 0.0 0.0 4.4166066085150515 1.3521535072833748E-4 78-79 0.0 0.0 0.0 5.007970944925436 1.3521535072833748E-4 80-81 0.0 0.0 0.0 5.8975527373671675 1.3521535072833748E-4 82-83 0.0 0.0 0.0 6.849942060222213 1.3521535072833748E-4 84-85 0.0 0.0 0.0 7.9020527042394075 1.3521535072833748E-4 86-87 0.0 0.0 0.0 8.804209524298875 1.3521535072833748E-4 88-89 0.0 0.0 0.0 10.221739653659402 1.3521535072833748E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3490 0.0 31.754923 1 GCGGTAT 65 0.0058042677 29.270302 1 GGTATCA 2240 0.0 28.028929 1 AATCGAT 35 0.008393921 27.141802 70-71 AACGCGA 35 0.008398084 27.139044 76-77 TCAACGC 4100 0.0 26.66971 4 ATCAACG 4095 0.0 26.58257 3 TATCAAC 4140 0.0 26.410238 2 CAACGCA 4160 0.0 26.39754 5 AACGCAG 4220 0.0 26.115383 6 CCTATAC 185 8.885763E-9 25.694706 3 GTACATG 2980 0.0 22.66484 1 CGCAGAG 4770 0.0 22.606241 8 ACGCAGA 4865 0.0 22.555372 7 TACATGG 2910 0.0 22.380636 2 GTATAGG 240 5.828042E-9 21.800278 1 GCAGAGT 4910 0.0 21.38263 9 AGAGTAC 3670 0.0 21.098055 10-11 ACATGGG 3000 0.0 21.07394 3 GGACCGA 115 0.0037206784 20.653471 6 >>END_MODULE