FastQCFastQC Report
Thu 26 May 2016
SRR937214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739561
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.4535122863428439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.36954355354054635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.23000131158890208No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8700.11763735513365361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.037.1902731
GTATCAA37850.034.7775151
TATTCCG550.002577746834.548975
GTATAGG1551.3496901E-930.6586441
TCAACGC45500.028.3984954
ATCAACG45550.028.3673213
CAACGCA46200.027.865395
TATCAAC46850.027.7829782
GATCGGA1205.3740187E-627.7111517
TCTACGC1056.897779E-527.145622
CCGCTCA700.00838550627.1456189
AGCGATC1405.796246E-727.1456188
CGCATTC1056.904133E-527.1419496
AACGCAG47750.026.9572126
ATAGCCC3750.024.0691173
CGCAGAG53700.023.796658
ACGCATT1201.7088333E-423.7524175
TAGTTCG500.001652889523.75241794-95
ACGCAGA54600.023.4043967
TACATGG26800.023.3979022
Produced by FastQC (version 0.11.5)