##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937214_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739561 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21601869217008 33.0 31.0 34.0 31.0 34.0 2 32.42694923069226 34.0 31.0 34.0 31.0 34.0 3 32.51907956206452 34.0 31.0 34.0 31.0 34.0 4 36.12906981303774 37.0 35.0 37.0 35.0 37.0 5 35.98917330686718 37.0 35.0 37.0 35.0 37.0 6 36.07133285827673 37.0 35.0 37.0 35.0 37.0 7 36.03458267810228 37.0 35.0 37.0 35.0 37.0 8 36.01079424144864 37.0 35.0 37.0 35.0 37.0 9 37.72508285320616 39.0 37.0 39.0 35.0 39.0 10-11 37.645589748512975 39.0 37.0 39.0 35.0 39.0 12-13 37.57020651440517 39.0 37.0 39.0 35.0 39.0 14-15 38.8131432025215 40.0 38.0 41.0 35.0 41.0 16-17 38.7864185645268 40.0 38.0 41.0 35.0 41.0 18-19 38.808364015950005 40.0 38.0 41.0 35.0 41.0 20-21 38.72491383401775 40.0 38.0 41.0 34.0 41.0 22-23 38.6014574862655 40.0 38.0 41.0 34.0 41.0 24-25 38.456905515569375 40.0 38.0 41.0 34.0 41.0 26-27 38.249023407129364 40.0 38.0 41.0 34.0 41.0 28-29 38.24267301818241 40.0 38.0 41.0 34.0 41.0 30-31 38.35944972760868 40.0 38.0 41.0 34.0 41.0 32-33 38.31384970272905 40.0 38.0 41.0 34.0 41.0 34-35 38.20190626601457 40.0 38.0 41.0 34.0 41.0 36-37 38.05492582761936 40.0 38.0 41.0 33.5 41.0 38-39 37.907265932086744 40.0 37.0 41.0 33.0 41.0 40-41 37.7613476102715 40.0 37.0 41.0 33.0 41.0 42-43 37.556103553324206 40.0 37.0 41.0 33.0 41.0 44-45 37.24704250224119 40.0 37.0 41.0 32.5 41.0 46-47 36.907995418903916 40.0 37.0 41.0 31.0 41.0 48-49 36.59181527960506 40.0 36.0 41.0 30.5 41.0 50-51 36.38597627511456 39.5 36.0 41.0 30.0 41.0 52-53 36.16807538526234 39.0 36.0 41.0 30.0 41.0 54-55 35.971518914599336 39.0 35.0 41.0 29.0 41.0 56-57 35.73406318072478 39.0 35.0 40.0 28.5 41.0 58-59 35.51071446439171 39.0 35.0 40.0 28.0 41.0 60-61 35.200750850842596 38.0 35.0 40.0 28.0 41.0 62-63 34.87027642074149 38.0 34.0 40.0 26.5 41.0 64-65 34.54399231435947 37.5 34.0 40.0 26.0 41.0 66-67 34.17085608895006 37.0 34.0 39.5 26.0 41.0 68-69 33.746634827958744 36.5 33.5 39.0 26.0 40.5 70-71 33.35313787503668 36.0 33.0 39.0 25.0 40.0 72-73 32.928031629574846 35.5 33.0 38.5 24.0 40.0 74-75 32.50673372446627 35.0 32.0 37.5 23.0 39.5 76-77 31.13633899029289 34.0 30.5 36.0 21.5 39.0 78-79 31.74076107312311 35.0 32.0 36.0 22.0 39.0 80-81 31.56910653752699 35.0 32.0 36.0 21.5 38.5 82-83 31.237799180865405 35.0 32.0 36.0 20.0 37.0 84-85 30.914893430021323 34.0 31.0 35.0 19.5 37.0 86-87 30.601696141359536 34.0 31.0 35.0 18.0 36.5 88-89 30.253670758733897 34.0 31.0 35.0 15.5 36.0 90-91 29.83933171165056 34.0 30.5 35.0 8.5 36.0 92-93 29.464129395682033 34.0 30.0 35.0 4.0 35.0 94-95 29.096874361952565 34.0 30.0 35.0 2.0 35.0 96-97 28.715879420358835 34.0 29.0 35.0 2.0 35.0 98-99 28.13807853578001 34.0 29.0 35.0 2.0 35.0 100-101 26.569963126773857 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04135182943393545 1101 2 0.062414362685061064 1101 3 0.11975045661886696 1101 4 0.1035648084793479 1101 5 -0.05157037426332067 1101 6 0.07168394866472738 1101 7 0.08096348040776036 1101 8 0.0765606586333547 1101 9 0.01818665410200282 1101 10-11 0.17701085707875563 1101 12-13 0.1679401284639681 1101 14-15 0.24531640127870702 1101 16-17 0.2002594380330791 1101 18-19 0.215917256158221 1101 20-21 0.0733508716505682 1101 22-23 0.08683242924480794 1101 24-25 0.006469909584225775 1101 26-27 -0.051096359919590384 1101 28-29 -0.10200387110355535 1101 30-31 0.048353244747076474 1101 32-33 0.08421629204931946 1101 34-35 0.14147078446409012 1101 36-37 0.08107039064129395 1101 38-39 0.04843541493033143 1101 40-41 0.16222999111184322 1101 42-43 0.018693474203274718 1101 44-45 0.14503896780068004 1101 46-47 0.28493456376659765 1101 48-49 0.23965294747220156 1101 50-51 0.27732561243459486 1101 52-53 0.396032306952236 1101 54-55 0.32653648376925304 1101 56-57 0.3881727460997766 1101 58-59 0.5582040734304741 1101 60-61 0.5153900872297541 1101 62-63 0.3551179543695113 1101 64-65 0.5226890848195893 1101 66-67 0.4410954927058768 1101 68-69 0.4213177788987892 1101 70-71 0.2757659611431791 1101 72-73 0.3140816053975968 1101 74-75 0.324356441217283 1101 76-77 0.3338981182373608 1101 78-79 0.3698121107372536 1101 80-81 0.3930764586403228 1101 82-83 0.21094036286106288 1101 84-85 0.15310336751974418 1101 86-87 0.1073398880480454 1101 88-89 0.09177219983862628 1101 90-91 0.22769101249033596 1101 92-93 0.2833289459638024 1101 94-95 0.31706384444314395 1101 96-97 0.2812467507160932 1101 98-99 0.1070518370466722 1101 100-101 0.35937646722327443 1102 1 0.04181892449969382 1102 2 -0.061223607595565 1102 3 0.01624377831452506 1102 4 -0.05498761554190423 1102 5 -0.17779722075076165 1102 6 -0.07465830712288835 1102 7 -0.05881923014418078 1102 8 -0.013621898289791545 1102 9 0.016952137003940493 1102 10-11 0.003373861416896773 1102 12-13 -0.11217577175528248 1102 14-15 -0.08392425519561897 1102 16-17 -0.1161523477789288 1102 18-19 4.8752508801896965E-4 1102 20-21 -0.08780992095272921 1102 22-23 -0.04182338377855643 1102 24-25 -0.15740140608613018 1102 26-27 -0.1175213211333741 1102 28-29 -0.10572763671279972 1102 30-31 0.009276085984566862 1102 32-33 -0.13568369040754646 1102 34-35 -0.13663198655753206 1102 36-37 -0.2262124661034619 1102 38-39 -0.03431080084160243 1102 40-41 -0.0636898865626776 1102 42-43 0.005615173331975143 1102 44-45 -0.10992346606060721 1102 46-47 0.02344795972099689 1102 48-49 0.21571089613794214 1102 50-51 0.06868175473470473 1102 52-53 0.30844367981465837 1102 54-55 0.051588950745916407 1102 56-57 0.1441390372855409 1102 58-59 0.12504812071175309 1102 60-61 0.12760444271436455 1102 62-63 0.3940702175425628 1102 64-65 0.4809255879741059 1102 66-67 0.25117081268764707 1102 68-69 0.10069780585602217 1102 70-71 0.14229336561790262 1102 72-73 0.30840788032527655 1102 74-75 0.31924727308688716 1102 76-77 0.22939660685634067 1102 78-79 0.19117387694859644 1102 80-81 0.28434185310462823 1102 82-83 0.34728736179331676 1102 84-85 0.262977194096802 1102 86-87 0.29986499531025146 1102 88-89 0.327314813419612 1102 90-91 0.3766542708069025 1102 92-93 0.24512280759826055 1102 94-95 0.18366596968959925 1102 96-97 0.04590122670689922 1102 98-99 0.14114364035850357 1102 100-101 0.03977623418110099 1103 1 -0.05213379200507262 1103 2 -0.1424364769277915 1103 3 -0.07857964716965427 1103 4 -0.05586709986377514 1103 5 -0.18493254920695534 1103 6 -0.11637628516697873 1103 7 -0.16167014390109102 1103 8 -0.1466882662047979 1103 9 -0.1971866947639711 1103 10-11 -0.1861457470858312 1103 12-13 -0.057235790222506466 1103 14-15 -0.11889740752288702 1103 16-17 -0.3292201002479658 1103 18-19 -0.22635507744453776 1103 20-21 -0.21912122037618786 1103 22-23 -0.13200562814064654 1103 24-25 -0.07780758743735561 1103 26-27 -0.3235975632587227 1103 28-29 -0.2510292696090204 1103 30-31 -0.2590860743487369 1103 32-33 -0.11603131097344033 1103 34-35 -0.05369720002748579 1103 36-37 -0.016592448182265684 1103 38-39 0.06888722646142043 1103 40-41 0.14090137261121072 1103 42-43 0.06504618012701258 1103 44-45 0.12344945381758521 1103 46-47 -0.006722642686767699 1103 48-49 0.1933600988980828 1103 50-51 0.06623392170802589 1103 52-53 0.2749355221588061 1103 54-55 -0.07573834320172779 1103 56-57 0.008467855217666909 1103 58-59 -0.015324694837374864 1103 60-61 0.0853497062232762 1103 62-63 0.17383398532360417 1103 64-65 0.056068845439725123 1103 66-67 -0.00671261371306997 1103 68-69 -0.04437859325044968 1103 70-71 0.12046957644453471 1103 72-73 0.03311904967866042 1103 74-75 0.008983340036898824 1103 76-77 -0.08797194095123828 1103 78-79 0.22808394236389873 1103 80-81 0.06016597964116954 1103 82-83 -0.010174750665623122 1103 84-85 -0.2629807564122153 1103 86-87 0.0554658576339051 1103 88-89 0.07003201045822749 1103 90-91 0.08849006269867843 1103 92-93 -0.10968037609598369 1103 94-95 -0.2828219725023473 1103 96-97 -0.11456761058947862 1103 98-99 -0.14298634781428632 1103 100-101 -0.14502077752228004 1104 1 -0.10666369261787167 1104 2 0.0067852796215746025 1104 3 -0.06196245740073891 1104 4 -0.06600631971254245 1104 5 -0.22526584082010004 1104 6 -0.11421088610531882 1104 7 -0.09276257679133693 1104 8 -0.0869733163237072 1104 9 -0.15487270703942357 1104 10-11 -0.1641332869275942 1104 12-13 -0.0831276199118065 1104 14-15 -0.1827074162355231 1104 16-17 -0.16887424397359752 1104 18-19 -0.19036291273811656 1104 20-21 -0.24967011622924673 1104 22-23 -0.4112655525825559 1104 24-25 -0.42918672305184913 1104 26-27 -0.1562998281786534 1104 28-29 -0.39256458858007903 1104 30-31 -0.40635326053295984 1104 32-33 -0.41998857168286463 1104 34-35 -0.4370499960291596 1104 36-37 -0.4078261059204067 1104 38-39 -0.6364404815329436 1104 40-41 -0.4473623345957236 1104 42-43 -0.37968936048758906 1104 44-45 -0.43578915687598396 1104 46-47 -0.42822328124860576 1104 48-49 -0.5264163619260529 1104 50-51 -0.6411188354088111 1104 52-53 -0.43299929228156486 1104 54-55 -0.4571930733592282 1104 56-57 -0.49988934570634314 1104 58-59 -0.33188914705337424 1104 60-61 -0.36090720342902927 1104 62-63 -0.355199009671189 1104 64-65 -0.1815466394723515 1104 66-67 -0.29143342669768657 1104 68-69 -0.4292545810163162 1104 70-71 -0.40134845955552834 1104 72-73 -0.3618730700671762 1104 74-75 -0.5321325088129001 1104 76-77 -0.32623084831176996 1104 78-79 -0.3868559744954716 1104 80-81 -0.25726305250321957 1104 82-83 -0.30724757344921727 1104 84-85 -0.2593912003919314 1104 86-87 -0.13949600485231883 1104 88-89 -0.19002609806204518 1104 90-91 -0.2842793757678983 1104 92-93 -0.2816041909896043 1104 94-95 -0.3279812362666661 1104 96-97 -0.24451281943140302 1104 98-99 -0.02196824571000988 1104 100-101 0.14041089879065893 1105 1 -0.2348351705284486 1105 2 -0.13700285106389742 1105 3 -0.11068294236095255 1105 4 -0.17050740536620168 1105 5 -0.290144683068867 1105 6 -0.34186145975842663 1105 7 -0.17776229995340032 1105 8 -0.22943262488727356 1105 9 -0.32977293314868206 1105 10-11 -0.14951923642935583 1105 12-13 -0.2594464534214609 1105 14-15 -0.2678964366930998 1105 16-17 -0.2505361994071791 1105 18-19 -0.260292925113518 1105 20-21 -0.23598235052093486 1105 22-23 -0.3272055820021933 1105 24-25 -0.3051595839549961 1105 26-27 -0.22333501353287488 1105 28-29 -0.4547064762557014 1105 30-31 -0.27605087867916467 1105 32-33 -0.17577785161989823 1105 34-35 -0.2514361415621096 1105 36-37 -0.17835438753492383 1105 38-39 -0.009194540201640677 1105 40-41 -0.2590653967226473 1105 42-43 -0.468173500666353 1105 44-45 -0.26338570971985575 1105 46-47 -0.30093693654197295 1105 48-49 -0.45854318063910426 1105 50-51 -0.42964382788930067 1105 52-53 -0.3228566982950838 1105 54-55 -0.4253462350729862 1105 56-57 -0.3026206138785099 1105 58-59 -0.18622300762582 1105 60-61 -0.5712958090786202 1105 62-63 -0.4515850025691748 1105 64-65 -0.4351758352506465 1105 66-67 -0.3769434295259231 1105 68-69 -0.6275266633314587 1105 70-71 -0.6927720201104606 1105 72-73 -0.6509390188506643 1105 74-75 -0.6541469612499 1105 76-77 -0.6152361920322669 1105 78-79 -0.6445801271843159 1105 80-81 -0.48195594944019504 1105 82-83 -0.6916595023213183 1105 84-85 -0.8606727356004775 1105 86-87 -0.9298645883847989 1105 88-89 -0.8125545712184952 1105 90-91 -0.9107217388565694 1105 92-93 -0.6176914383375696 1105 94-95 -0.346079703781605 1105 96-97 -0.6421456305635793 1105 98-99 -0.7205209070204859 1105 100-101 -0.3165198513204466 1106 1 -0.19808816992312472 1106 2 -0.02275565473171781 1106 3 -0.1857160249729617 1106 4 0.002423475366448713 1106 5 -0.13374842498438966 1106 6 -0.0459568293384649 1106 7 -0.15127329598348638 1106 8 -0.09278281937293542 1106 9 -0.24852432949269598 1106 10-11 -0.2282921340842634 1106 12-13 -0.40231655380547693 1106 14-15 -0.1743953320298317 1106 16-17 -0.10482185367907704 1106 18-19 -0.11432652138917376 1106 20-21 -0.3541507672720954 1106 22-23 -0.29959568376106915 1106 24-25 -0.20905674429253196 1106 26-27 -0.08797986954474624 1106 28-29 -0.1507140303574701 1106 30-31 -0.189964480072355 1106 32-33 -0.160252415721871 1106 34-35 -0.24861290105104672 1106 36-37 -0.31852823117112905 1106 38-39 -0.044602005319298144 1106 40-41 -0.2633026444020956 1106 42-43 -0.46641959710908765 1106 44-45 -0.36488297240821055 1106 46-47 -0.14693045559621964 1106 48-49 -0.3171863829977539 1106 50-51 -0.15789761973903893 1106 52-53 -0.12733798394362594 1106 54-55 -0.11519364450874292 1106 56-57 0.01061777760957483 1106 58-59 0.06345953296602147 1106 60-61 0.026724198979600544 1106 62-63 0.19821191888611622 1106 64-65 0.21696026079109032 1106 66-67 0.0899710941703944 1106 68-69 0.05614055137039742 1106 70-71 0.25448579893863155 1106 72-73 0.14293100706166229 1106 74-75 0.06272984922349423 1106 76-77 -0.17821441002041993 1106 78-79 -0.10353286440048848 1106 80-81 0.028312549611722915 1106 82-83 -0.09249282133317749 1106 84-85 -0.10891358083496172 1106 86-87 0.21887158900292292 1106 88-89 0.2849785066196908 1106 90-91 0.18323860783774748 1106 92-93 0.045843598678676045 1106 94-95 -0.03947215617223776 1106 96-97 0.25010434986373653 1106 98-99 0.13316897380572712 1106 100-101 0.24890733924494413 1107 1 -0.28411108089799697 1107 2 -0.19383414377514896 1107 3 -0.17199035535636398 1107 4 -0.028921160946879354 1107 5 -0.18593211366005846 1107 6 -0.2638747106179551 1107 7 -0.14522746531903152 1107 8 -0.13982918599210592 1107 9 -0.007723796152390605 1107 10-11 -0.15357764979906108 1107 12-13 -0.20782926203259677 1107 14-15 -0.15981453140934576 1107 16-17 -0.23138342093267994 1107 18-19 -0.2505091294406938 1107 20-21 -0.2946619722115926 1107 22-23 -0.41519414474312555 1107 24-25 -0.24875660965024338 1107 26-27 -0.18103664152869214 1107 28-29 -0.2461771518797562 1107 30-31 -0.2615536511726262 1107 32-33 -0.11934883092555992 1107 34-35 -0.38265495824569484 1107 36-37 -0.29097994077953615 1107 38-39 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-0.05538399451816289 2316 100-101 -0.3735059185691938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 1.0 4 5.0 5 11.0 6 27.0 7 46.0 8 149.0 9 304.0 10 571.0 11 961.0 12 1444.0 13 2068.0 14 2774.0 15 3966.0 16 5491.0 17 7733.0 18 6607.0 19 3953.0 20 3127.0 21 3259.0 22 3326.0 23 3456.0 24 4163.0 25 4872.0 26 5906.0 27 7302.0 28 8918.0 29 11244.0 30 14230.0 31 18162.0 32 22969.0 33 30510.0 34 42510.0 35 62730.0 36 99869.0 37 168353.0 38 168860.0 39 19679.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00549227564189 19.92072725305051 15.792862747220044 22.28091772408755 2 15.324902205497587 20.8161328139261 40.54716243825729 23.311802542319025 3 19.0649588066434 24.62230972157807 30.911986976057413 25.40074449572111 4 12.627626389168709 17.590705837652337 38.658744849985325 31.122922923193624 5 13.11307372233063 37.837647787464626 34.92943118983193 14.119847300372824 6 31.124680722753094 37.2457444348742 17.746068275639196 13.883506566733509 7 27.83448559348046 33.35032539574153 21.125775967093993 17.689413043684024 8 26.163088643127473 34.7177582376572 20.316241662283435 18.802911456931884 9 25.90739641490019 16.87960830817201 21.13172544252604 36.08126983440176 10-11 24.314356760294284 26.99540673453576 28.08639179188735 20.60384471328261 12-13 25.46611441506495 24.342274292174093 28.57841188449774 21.61319940826322 14-15 22.4625825320697 25.74615210915665 27.300452565778887 24.490812792994763 16-17 21.26809012373557 29.010723929466266 27.952866633043115 21.768319313755054 18-19 21.120367353064857 27.34169324775103 30.772850380157955 20.765089019026153 20-21 21.94247668549315 26.256928096532945 30.983651111943438 20.81694410603047 22-23 21.55058119920465 26.297463925624893 30.880327659375954 21.271627215794503 24-25 21.514945413341483 26.40705664734882 30.986210682596905 21.091787256712795 26-27 21.41045213057253 26.776333492440603 30.89813574058574 20.91507863640113 28-29 21.15490710441626 27.129769856938097 30.748505827947103 20.966817210698544 30-31 21.469503711045515 26.60459279912487 30.648408692324647 21.277494797504968 32-33 21.154644444474492 26.617885475302238 30.674684035529186 21.552786044694084 34-35 21.671572730308927 26.70131334670162 30.602681861266344 21.024432061723104 36-37 21.628864808439634 26.537945637799876 30.675552154124492 21.157637399636 38-39 21.37673051349018 26.921878179692833 30.557908692574536 21.143482614242455 40-41 21.586010600897833 26.941830277462277 30.416058196765643 21.05610092487425 42-43 21.429914074522838 26.843124474291326 30.20225077689938 21.524710674286457 44-45 21.634050470481814 26.974245532146774 30.115838990968967 21.275865006402448 46-47 21.7110556127216 27.261172506392306 29.695251642528476 21.33252023835762 48-49 21.648465541358686 27.43710622727958 29.363317808346984 21.551110423014748 50-51 21.75013284908209 27.472514099580696 29.469103968435327 21.308249082901884 52-53 21.827564572352674 27.60175672496275 28.971088136427436 21.59959056625714 54-55 21.70713436755048 27.488604726317366 29.20732704942527 21.596933856706883 56-57 21.84498641761802 27.537485075605662 28.947105106948584 21.670423399827733 58-59 21.81145301063739 27.23027579875088 29.210775040868842 21.747496149742886 60-61 21.70848652105776 27.311945870590797 29.432595823738676 21.546971784612765 62-63 21.305477168211954 27.47197323817778 29.694778388800923 21.52777120480934 64-65 21.281544051133036 27.393548334755348 29.463492531380105 21.86141508273151 66-67 21.37984561111254 27.277465956155073 29.200701497239578 22.141986935492813 68-69 21.650141097218484 26.976070939381607 29.342407184802877 22.031380778597033 70-71 21.595649310874965 26.97580050868015 29.434286015622778 21.994264164822102 72-73 21.304395445406126 26.80989127333648 29.699646141427145 22.18606713983025 74-75 21.155996597981776 27.05496909653159 29.189411015453764 22.599623290032874 76-77 21.018144547914236 27.063555271302842 29.408189452932216 22.51011072785071 78-79 21.356926609164084 26.894806513593878 29.13958415871037 22.608682718531668 80-81 21.133956495813056 27.056591680740333 28.98327521326841 22.8261766101782 82-83 21.04792038509384 26.668830115203633 29.56852723240846 22.714722267294064 84-85 20.827572592100307 26.69315086460134 29.679721942964775 22.799554600333575 86-87 20.540158283089564 26.788053994193856 29.483233972586444 23.188553750130144 88-89 20.480460705743 26.475436103309935 29.265942903966003 23.77816028698106 90-91 20.625971086142524 26.384469653431275 29.5938375160749 23.395721744351306 92-93 19.98458511652435 26.356205504736018 29.78709882427709 23.87211055446254 94-95 19.8245986470352 26.563664119660174 29.615474585598754 23.99626264770587 96-97 19.793363901016956 26.70239506950745 29.48634392565319 24.017897103822403 98-99 19.83845549007266 26.864093429977885 29.033187140532362 24.26426393941709 100-101 19.479444663442326 26.244743769097045 28.902897240559067 25.372914326901558 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 28.0 2 34.5 3 51.0 4 42.0 5 36.5 6 93.5 7 235.5 8 375.5 9 483.0 10 480.0 11 442.5 12 512.0 13 588.5 14 640.5 15 692.0 16 772.0 17 896.0 18 1008.5 19 1098.0 20 1262.5 21 1496.0 22 1733.5 23 2010.0 24 2437.0 25 3138.5 26 4000.5 27 5194.0 28 6556.0 29 7855.0 30 9601.0 31 11508.5 32 13609.5 33 15472.5 34 17497.0 35 19843.5 36 22173.0 37 24407.5 38 25292.5 39 25498.5 40 26297.5 41 26935.0 42 27067.0 43 27666.5 44 28511.5 45 29052.5 46 29191.0 47 30290.0 48 31023.5 49 29527.0 50 27506.0 51 25368.0 52 23538.0 53 22388.5 54 21274.5 55 19295.5 56 16564.5 57 14098.0 58 12485.5 59 10647.5 60 8206.5 61 6299.0 62 4982.5 63 3712.5 64 2489.0 65 1702.5 66 1180.0 67 835.5 68 609.0 69 458.5 70 316.0 71 223.5 72 168.5 73 143.5 74 117.0 75 94.0 76 78.5 77 47.0 78 24.5 79 14.0 80 6.5 81 4.0 82 4.0 83 2.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046108434598363085 2 0.0 3 0.0 4 0.0 5 0.009465074550983625 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005814260081318512 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0016901918841042186 24-25 4.056460521850125E-4 26-27 0.002433876313110075 28-29 0.0028395223652950873 30-31 0.00162258420874005 32-33 0.0 34-35 0.0 36-37 4.056460521850125E-4 38-39 6.084690782775187E-4 40-41 1.3521535072833748E-4 42-43 0.011222874110452011 44-45 0.0 46-47 0.0 48-49 6.760767536416874E-5 50-51 0.0 52-53 4.056460521850125E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3521535072833748E-4 84-85 6.760767536416874E-5 86-87 0.0 88-89 0.0 90-91 0.007301628939330224 92-93 0.0021634456116533998 94-95 0.0 96-97 0.0 98-99 0.0016901918841042186 100-101 0.011020051084359505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 739561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7523186915459 #Duplication Level Percentage of deduplicated Percentage of total 1 83.26108447502895 54.74609361005829 2 10.292344930006925 13.53491087842264 3 2.815955666475447 5.554668432100743 4 1.2072808368778716 3.175260573463602 5 0.6237818258839757 2.050755070475878 6 0.3796400634496427 1.4977328664011766 7 0.23950067766881308 1.1023407419444706 8 0.18293683243957853 0.9622816725591281 9 0.13104514461384845 0.7754869900466534 >10 0.7800608081422732 9.540290509249449 >50 0.058888155440519806 2.628217227372764 >100 0.026034237448608884 2.9804539826991596 >500 8.2648372774938E-4 0.3934449233729636 >1k 6.19862795812035E-4 1.058062521833094 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3354 0.4535122863428439 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2733 0.36954355354054635 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1701 0.23000131158890208 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 870 0.11763735513365361 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3521535072833748E-4 0.0 12-13 0.0 0.0 0.0 1.3521535072833748E-4 0.0 14-15 0.0 0.0 0.0 1.3521535072833748E-4 0.0 16-17 0.0 0.0 0.0 1.3521535072833748E-4 0.0 18-19 0.0 0.0 0.0 2.7043070145667497E-4 0.0 20-21 0.0 0.0 0.0 2.7043070145667497E-4 0.0 22-23 0.0 0.0 0.0 2.7043070145667497E-4 0.0 24-25 0.0 0.0 0.0 2.7043070145667497E-4 0.0 26-27 0.0 0.0 0.0 4.0564605218501245E-4 0.0 28-29 0.0 0.0 0.0 0.0016225842087400498 0.0 30-31 0.0 0.0 0.0 0.003447991443572606 0.0 32-33 0.0 0.0 0.0 0.0054086140291335 0.0 34-35 0.0 0.0 0.0 0.008315744069792755 0.0 36-37 0.0 0.0 0.0 0.01183134318872953 0.0 38-39 0.0 0.0 0.0 0.018186464672961394 0.0 40-41 0.0 0.0 0.0 0.03427709140963355 0.0 42-43 0.0 0.0 0.0 0.06612030650615704 0.0 44-45 0.0 0.0 0.0 0.09221686919672617 0.0 46-47 0.0 0.0 0.0 0.15948650618407406 0.0 48-49 0.0 0.0 0.0 0.23466624118902973 0.0 50-51 0.0 0.0 0.0 0.2881439124020872 0.0 52-53 0.0 0.0 0.0 0.37197742985365645 0.0 54-55 0.0 0.0 0.0 0.4581096082676074 0.0 56-57 0.0 0.0 0.0 0.6639749797515013 0.0 58-59 0.0 0.0 0.0 0.873829204081881 0.0 60-61 0.0 0.0 0.0 1.114985782105871 0.0 62-63 0.0 0.0 0.0 1.3592523131966125 0.0 64-65 0.0 0.0 0.0 1.6400945966593694 0.0 66-67 0.0 0.0 0.0 2.047566056079215 1.3521535072833748E-4 68-69 0.0 0.0 0.0 2.6035715782741384 1.3521535072833748E-4 70-71 0.0 0.0 0.0 2.97595465417998 1.3521535072833748E-4 72-73 0.0 0.0 0.0 3.3469179689031736 1.3521535072833748E-4 74-75 0.0 0.0 0.0 3.8553276876417226 1.3521535072833748E-4 76-77 0.0 0.0 0.0 4.440336902567875 1.3521535072833748E-4 78-79 0.0 0.0 0.0 5.038867652566861 1.3521535072833748E-4 80-81 0.0 0.0 0.0 5.940416003548051 1.3521535072833748E-4 82-83 0.0 0.0 0.0 6.9077466226585775 1.3521535072833748E-4 84-85 0.0 0.0 0.0 7.971688609864501 1.3521535072833748E-4 86-87 0.0 0.0 0.0 8.884189404254686 1.3521535072833748E-4 88-89 0.0 0.0 0.0 10.320717290392544 1.3521535072833748E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2415 0.0 37.190273 1 GTATCAA 3785 0.0 34.777515 1 TATTCCG 55 0.0025777468 34.54897 5 GTATAGG 155 1.3496901E-9 30.658644 1 TCAACGC 4550 0.0 28.398495 4 ATCAACG 4555 0.0 28.367321 3 CAACGCA 4620 0.0 27.86539 5 TATCAAC 4685 0.0 27.782978 2 GATCGGA 120 5.3740187E-6 27.711151 7 TCTACGC 105 6.897779E-5 27.14562 2 CCGCTCA 70 0.008385506 27.145618 9 AGCGATC 140 5.796246E-7 27.145618 8 CGCATTC 105 6.904133E-5 27.141949 6 AACGCAG 4775 0.0 26.957212 6 ATAGCCC 375 0.0 24.069117 3 CGCAGAG 5370 0.0 23.79665 8 ACGCATT 120 1.7088333E-4 23.752417 5 TAGTTCG 50 0.0016528895 23.752417 94-95 ACGCAGA 5460 0.0 23.404396 7 TACATGG 2680 0.0 23.397902 2 >>END_MODULE