FastQCFastQC Report
Thu 26 May 2016
SRR937213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922124
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.2981160885087038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.20713049438036532No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12440.1349059345597772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.13382148170961822No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11450.12416985134320331No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44450.029.4164921
GGTATCA30800.026.5526371
ATACCGT950.001225016125.0047346
TACATGG35550.025.0042532
GTACATG36450.024.5239431
ATCAACG52400.024.4931353
TCAACGC52500.024.445154
CAACGCA52550.024.4192375
TATCAAC54000.023.9434682
AACGCAG54000.023.7544966
ACATGGG35500.023.432663
GAGTACT26600.022.50364912-13
AGAGTAC43350.021.64422210-11
ACGCAGA61100.020.7620147
CGCAGAG61100.020.7620148
AGTACTT28750.020.49027812-13
GTACTTT29200.020.33609614-15
GTATCGG1101.3446079E-619.43338644-45
GCAGAGT64150.019.328419
CATGGGG19000.019.0128964
Produced by FastQC (version 0.11.5)