##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937213_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 922124 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73178336102303 33.0 31.0 34.0 30.0 34.0 2 31.832447696839036 33.0 31.0 34.0 30.0 34.0 3 31.89843231495981 33.0 31.0 34.0 30.0 34.0 4 35.52182678251515 37.0 35.0 37.0 33.0 37.0 5 35.49153476105166 37.0 35.0 37.0 33.0 37.0 6 35.56080852466697 37.0 35.0 37.0 33.0 37.0 7 35.5061054695464 37.0 35.0 37.0 33.0 37.0 8 35.49440422329318 37.0 35.0 37.0 33.0 37.0 9 37.1213784697069 39.0 37.0 39.0 34.0 39.0 10-11 37.03453440101332 39.0 37.0 39.0 33.0 39.0 12-13 36.939074354425216 39.0 37.0 39.0 33.0 39.0 14-15 38.11653204991953 40.0 38.0 41.0 33.0 41.0 16-17 38.06234139877067 40.0 38.0 41.0 33.0 41.0 18-19 38.02955893133678 40.0 38.0 41.0 33.0 41.0 20-21 37.953686272128266 40.0 38.0 41.0 33.0 41.0 22-23 37.82837720306597 40.0 38.0 41.0 33.0 41.0 24-25 37.69294693555314 40.0 37.0 41.0 32.0 41.0 26-27 37.53297821117333 40.0 37.0 41.0 32.0 41.0 28-29 37.36584884462393 39.5 37.0 41.0 32.0 41.0 30-31 37.37083841218752 40.0 37.0 41.0 32.0 41.0 32-33 37.23036706560072 39.5 37.0 41.0 31.5 41.0 34-35 37.05014130420638 39.0 36.0 41.0 31.0 41.0 36-37 37.08417468800292 39.5 37.0 41.0 31.0 41.0 38-39 37.07797975109638 40.0 37.0 41.0 31.0 41.0 40-41 36.94370659477467 40.0 36.5 41.0 31.0 41.0 42-43 36.72453270926687 39.5 36.0 41.0 30.0 41.0 44-45 36.517444508547655 39.0 36.0 41.0 30.0 41.0 46-47 36.233124286972256 39.0 36.0 41.0 30.0 41.0 48-49 35.96173399672929 39.0 35.5 41.0 28.5 41.0 50-51 35.03405453062712 38.0 34.0 40.0 27.0 40.5 52-53 35.15437620103153 38.5 35.0 40.0 27.5 40.5 54-55 35.538581036823686 39.0 35.0 40.0 28.0 41.0 56-57 35.33926131409659 39.0 35.0 40.0 27.0 41.0 58-59 35.117442448087246 38.5 35.0 40.0 26.0 41.0 60-61 34.85526024699498 38.0 34.0 40.0 26.0 41.0 62-63 34.56399790049928 38.0 34.0 40.0 26.0 41.0 64-65 34.23177305872095 37.5 34.0 40.0 25.0 41.0 66-67 33.85061607766417 37.0 34.0 40.0 23.5 41.0 68-69 33.36956743344713 36.5 33.0 39.0 22.5 41.0 70-71 32.95648958274592 36.0 33.0 39.0 22.0 40.5 72-73 32.53731276921542 35.5 32.0 38.5 20.5 40.0 74-75 32.08696823854493 35.0 32.0 37.5 19.5 39.5 76-77 31.656839535680668 35.0 31.5 37.0 18.0 39.0 78-79 31.185566149454953 35.0 31.0 36.5 16.0 39.0 80-81 30.749213771683635 35.0 31.0 36.0 11.5 38.5 82-83 30.310001691746443 34.0 30.5 35.5 7.0 37.0 84-85 29.857061523179098 34.0 30.0 35.0 4.5 37.0 86-87 29.45169413224252 34.0 29.5 35.0 2.0 36.0 88-89 29.079238800855414 34.0 29.0 35.0 2.0 36.0 90-91 28.662782879525963 34.0 29.0 35.0 2.0 35.5 92-93 28.24122514976294 34.0 29.0 35.0 2.0 35.0 94-95 27.732765875305276 34.0 27.5 35.0 2.0 35.0 96-97 27.15035938767454 33.0 26.5 35.0 2.0 35.0 98-99 26.47092961467221 33.0 25.0 35.0 2.0 35.0 100-101 24.660718623525685 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.048212085732942 1101 2 -0.036230128160518404 1101 3 0.08255856271069817 1101 4 0.1774807894316126 1101 5 0.14284639581921255 1101 6 0.02463850606792306 1101 7 0.11899870147351521 1101 8 -0.01571299271157045 1101 9 -0.0181761069339359 1101 10-11 0.06180174461930932 1101 12-13 0.1334187525931796 1101 14-15 0.20311067759570278 1101 16-17 0.07577710006740546 1101 18-19 0.06796855001622504 1101 20-21 0.06934232345340519 1101 22-23 0.003764695224610648 1101 24-25 0.10279799384409216 1101 26-27 0.1611037294916926 1101 28-29 0.17338000887200877 1101 30-31 0.034178457011158514 1101 32-33 -0.006454606803281138 1101 34-35 -0.07421566257060164 1101 36-37 0.006212885947185498 1101 38-39 0.1505502959799827 1101 40-41 0.027035011840446543 1101 42-43 -0.1115894016158947 1101 44-45 -0.1369542282633276 1101 46-47 -0.1538212886754522 1101 48-49 0.15308452048669352 1101 50-51 -0.04375956800139136 1101 52-53 0.007384920065476308 1101 54-55 0.1441062148089216 1101 56-57 0.06753495537083865 1101 58-59 0.024806363749988236 1101 60-61 0.14258879997526464 1101 62-63 0.1055439183296798 1101 64-65 0.022591917069291867 1101 66-67 0.12706014488732364 1101 68-69 -0.05892035698929732 1101 70-71 0.035307432084984214 1101 72-73 0.2036643567639942 1101 74-75 0.27166278711607816 1101 76-77 0.2039954774808912 1101 78-79 0.23203128538822781 1101 80-81 0.25487122276376084 1101 82-83 0.21847164610055003 1101 84-85 0.3443760280573471 1101 86-87 0.3601977076671581 1101 88-89 0.24120453053239288 1101 90-91 0.16713919171596459 1101 92-93 0.179476423579775 1101 94-95 0.2352260128420518 1101 96-97 0.43564469828357133 1101 98-99 0.4079663116773453 1101 100-101 0.5362385783986277 1102 1 0.09505875399064934 1102 2 -0.12998675220883626 1102 3 -0.004279749413210965 1102 4 -0.173162351109184 1102 5 0.010003834329339156 1102 6 -0.043164940806278196 1102 7 -0.1497281844943501 1102 8 0.0212220772757874 1102 9 0.1691656952062104 1102 10-11 0.045655276156637115 1102 12-13 0.12127539135467913 1102 14-15 0.12731317335007475 1102 16-17 0.10170783340722522 1102 18-19 -0.03057733889676939 1102 20-21 0.13735181719531653 1102 22-23 0.17502517836555143 1102 24-25 0.12444974782974327 1102 26-27 0.36322230276208245 1102 28-29 0.28231178210948826 1102 30-31 0.273318446687 1102 32-33 0.1341893691469025 1102 34-35 0.11036960030903487 1102 36-37 0.06647738653446567 1102 38-39 0.21728017944661104 1102 40-41 0.19956918257818046 1102 42-43 0.22958493460770768 1102 44-45 0.4056394934480849 1102 46-47 0.376349770748341 1102 48-49 0.36895243602805294 1102 50-51 0.49306827033317546 1102 52-53 0.5342732570022903 1102 54-55 0.5173125783840717 1102 56-57 0.4414607685234273 1102 58-59 0.5998834832584166 1102 60-61 0.656654226246026 1102 62-63 0.5900944556101351 1102 64-65 0.6244027604337745 1102 66-67 0.75011657118052 1102 68-69 0.7974403503406151 1102 70-71 0.6392071106394042 1102 72-73 0.5608240022511666 1102 74-75 0.5671215266204683 1102 76-77 0.525058579567915 1102 78-79 0.8043135240858881 1102 80-81 0.5564309445599633 1102 82-83 0.6103007811899879 1102 84-85 0.40349028213651295 1102 86-87 0.507952015013025 1102 88-89 0.5764465249413853 1102 90-91 0.7294582383018842 1102 92-93 0.4004029299974654 1102 94-95 0.18298268925931538 1102 96-97 0.25307844533158175 1102 98-99 0.49514315163535016 1102 100-101 0.42393181357759246 1103 1 -0.06902713119727721 1103 2 -0.242801615095388 1103 3 0.047949633261847424 1103 4 -0.06365269959014341 1103 5 -0.23202869458497588 1103 6 -0.12021107793481889 1103 7 -0.061692345337910126 1103 8 -0.06536490210311285 1103 9 0.020217376235706297 1103 10-11 -0.13987549450596504 1103 12-13 0.0117015976059065 1103 14-15 -0.016835797607328118 1103 16-17 0.07094220156852771 1103 18-19 0.12375138122678919 1103 20-21 0.013442863946195871 1103 22-23 -0.1412953717987122 1103 24-25 -0.21037520676761545 1103 26-27 -0.22061033381056205 1103 28-29 -0.4726938271913923 1103 30-31 -0.22602755410786557 1103 32-33 -0.24120114384000857 1103 34-35 -0.26966053396203193 1103 36-37 -0.27768592209782383 1103 38-39 -0.25865768056737437 1103 40-41 -0.30937551424288046 1103 42-43 -0.26939507817043307 1103 44-45 -0.40031265393314897 1103 46-47 -0.18941362240075676 1103 48-49 -0.17918190547021595 1103 50-51 -0.09589448692747737 1103 52-53 -0.05798213471807401 1103 54-55 -0.05205912961031345 1103 56-57 -0.022090095007065713 1103 58-59 -0.0024460116026574497 1103 60-61 -0.00862772287283775 1103 62-63 0.2174532546663599 1103 64-65 0.3081752793871715 1103 66-67 0.27009750458831405 1103 68-69 0.250515907371863 1103 70-71 0.2632481214203253 1103 72-73 0.2803388354562202 1103 74-75 0.21171446242290415 1103 76-77 0.26035868974162923 1103 78-79 0.2911266362668705 1103 80-81 0.12157681153652788 1103 82-83 0.29188743356928626 1103 84-85 0.1275239983109202 1103 86-87 0.17175743515720754 1103 88-89 0.4968019986631944 1103 90-91 0.6456381786370322 1103 92-93 0.44808450281524514 1103 94-95 0.368888251774095 1103 96-97 0.059059519600737076 1103 98-99 0.1437279979706929 1103 100-101 0.4550231873280772 1104 1 0.014775331733108743 1104 2 0.06528039881095182 1104 3 0.10081258040099428 1104 4 0.16586686143859453 1104 5 0.30802952774894976 1104 6 0.24245960984268322 1104 7 0.1940859071543457 1104 8 0.1614110885142921 1104 9 0.3071571433463731 1104 10-11 0.14014670537675045 1104 12-13 0.07339632827525122 1104 14-15 -0.06952386831186175 1104 16-17 -0.0572380363471936 1104 18-19 -0.05351363510897755 1104 20-21 0.06128046970979284 1104 22-23 0.08903783587491176 1104 24-25 0.11329070594076995 1104 26-27 0.30882859556867714 1104 28-29 0.18867837687997735 1104 30-31 -0.11475483064300107 1104 32-33 0.04331024930734628 1104 34-35 0.1672739973272357 1104 36-37 0.22861695969827878 1104 38-39 0.19876880850022616 1104 40-41 0.2010804210842494 1104 42-43 0.005935825741765655 1104 44-45 0.11180931976208086 1104 46-47 0.04125781933501571 1104 48-49 0.3371601402699227 1104 50-51 0.19196050176315538 1104 52-53 0.3334625331214127 1104 54-55 0.4431469523198146 1104 56-57 0.4893753066851545 1104 58-59 0.2656793549048331 1104 60-61 0.14720602932620608 1104 62-63 0.31442799706395164 1104 64-65 0.6389943712863086 1104 66-67 0.7252537414987543 1104 68-69 0.5585005350002419 1104 70-71 0.46009720410442156 1104 72-73 0.47358462585581407 1104 74-75 0.6857029017292575 1104 76-77 0.7034987165490314 1104 78-79 0.5221645855003523 1104 80-81 0.8637880036283434 1104 82-83 0.6481044478944931 1104 84-85 0.5207666472176804 1104 86-87 0.7580144511578766 1104 88-89 0.5183955732631773 1104 90-91 0.49849274846194547 1104 92-93 0.5480185314656616 1104 94-95 0.3088260502159166 1104 96-97 0.5326594609595396 1104 98-99 0.7404566567626816 1104 100-101 0.8164257591635433 1105 1 -0.04628381892412037 1105 2 0.07258575775938425 1105 3 0.07428325785296508 1105 4 0.07671114859734018 1105 5 0.06949312734450075 1105 6 0.10820784037857578 1105 7 0.025721397548679192 1105 8 0.1470152341159121 1105 9 0.18200547499449726 1105 10-11 0.3154500420804993 1105 12-13 0.2478528500143824 1105 14-15 0.09465712258803194 1105 16-17 0.021806453036013806 1105 18-19 0.20823434264633534 1105 20-21 0.1284126739565039 1105 22-23 0.13696503506601942 1105 24-25 0.23995273829668662 1105 26-27 0.30319158849082584 1105 28-29 0.17148297748666153 1105 30-31 0.18745067997378584 1105 32-33 0.36811434435284696 1105 34-35 0.287344775891448 1105 36-37 0.20794582724123956 1105 38-39 -0.014837106565494196 1105 40-41 -0.08794258194911464 1105 42-43 -0.2197972379589359 1105 44-45 -0.26650210592041645 1105 46-47 -0.3870978429501193 1105 48-49 -0.21835047651470063 1105 50-51 -0.3840632596119704 1105 52-53 -0.31593711298576466 1105 54-55 -0.13362251178534024 1105 56-57 -0.07818662455852632 1105 58-59 0.010775411527689016 1105 60-61 0.0063440475264116 1105 62-63 0.02279503447547171 1105 64-65 -0.08143661961358362 1105 66-67 -0.190248027965346 1105 68-69 -0.00732984922625235 1105 70-71 0.010198316339007363 1105 72-73 -0.24847048031203656 1105 74-75 -0.5541226796660936 1105 76-77 -0.47227226423964197 1105 78-79 -0.14298329114272335 1105 80-81 -0.3877476384343481 1105 82-83 -0.22216855513887523 1105 84-85 -0.4576142931260989 1105 86-87 -0.31196153468641086 1105 88-89 -0.18142116081164517 1105 90-91 -0.13594228692733523 1105 92-93 -0.007353055996421887 1105 94-95 -0.056454536235047925 1105 96-97 -0.17788148949546212 1105 98-99 0.051894978299586114 1105 100-101 0.4998180746337191 1106 1 -0.14917749577470474 1106 2 0.11187744342590022 1106 3 0.013664242805084825 1106 4 -0.04773785581062384 1106 5 0.02549897929768008 1106 6 -0.254670256596647 1106 7 0.20706544679628536 1106 8 -0.33657839713503535 1106 9 -0.054067636420612075 1106 10-11 -0.36681549979516603 1106 12-13 -0.35876479704444364 1106 14-15 -0.22384682938034928 1106 16-17 -0.3290162403824368 1106 18-19 -0.13525915940565625 1106 20-21 -0.051397137565004414 1106 22-23 -0.040484045341337094 1106 24-25 -0.00991901272930562 1106 26-27 -0.07107691736113253 1106 28-29 0.04588923223661823 1106 30-31 -0.03627728020253329 1106 32-33 -0.009151387521299625 1106 34-35 0.021014406534398233 1106 36-37 -0.1666409260647228 1106 38-39 -0.12063301019826866 1106 40-41 0.17442156913767803 1106 42-43 0.08866529434080661 1106 44-45 -0.05216482061870664 1106 46-47 0.3228932271213196 1106 48-49 0.28411950372544936 1106 50-51 0.10965729062363039 1106 52-53 0.14850942144195756 1106 54-55 0.16883606854602107 1106 56-57 0.34301625581843354 1106 58-59 -0.05839353512074297 1106 60-61 0.10086829133246056 1106 62-63 0.2852684346882697 1106 64-65 0.02240229967505769 1106 66-67 0.13090165283720268 1106 68-69 -0.12877212768802337 1106 70-71 0.11216100063752776 1106 72-73 0.10890716066684547 1106 74-75 0.33929767297108526 1106 76-77 0.30883048693835136 1106 78-79 0.24888382392035524 1106 80-81 0.03665671633081402 1106 82-83 -0.3060053844442372 1106 84-85 -0.2956833385737383 1106 86-87 -0.3483509935707403 1106 88-89 -0.14783417950749822 1106 90-91 -0.2558550776250108 1106 92-93 -0.25913904170853996 1106 94-95 -0.8357143021569193 1106 96-97 -0.9084921446102214 1106 98-99 -0.6802736643512688 1106 100-101 -0.14022030505924832 1107 1 -0.36537976615654344 1107 2 -0.4806578162920232 1107 3 -0.44634607711235574 1107 4 -0.2603281550882315 1107 5 -0.31015633545980137 1107 6 -0.29569192842327396 1107 7 -0.4565052446381799 1107 8 -0.33305242534281376 1107 9 -0.5368677740195054 1107 10-11 -0.5589379628154632 1107 12-13 -0.2744424716230469 1107 14-15 -0.44434906536245933 1107 16-17 -0.4163055420680237 1107 18-19 -0.23261038059587946 1107 20-21 -0.5194053934989569 1107 22-23 -0.521822194636151 1107 24-25 -0.6017232782951893 1107 26-27 -0.5294532503504641 1107 28-29 -0.5642328867864279 1107 30-31 -0.40057036585785255 1107 32-33 -0.4879095574559429 1107 34-35 -0.35966154805796435 1107 36-37 -0.3319316037393065 1107 38-39 -0.43044553169809063 1107 40-41 -0.20578138923865907 1107 42-43 -0.15694874074691967 1107 44-45 -0.20227661972430866 1107 46-47 -0.33100254171252885 1107 48-49 -0.386632130261475 1107 50-51 -0.42779540975477204 1107 52-53 -0.42510598963475843 1107 54-55 -0.2695024247460296 1107 56-57 -0.19512443905600918 1107 58-59 -0.23413312920398255 1107 60-61 -0.459503225695407 1107 62-63 -0.40805801870009617 1107 64-65 -0.24948452523034348 1107 66-67 -0.20690364472041267 1107 68-69 -0.28597887003408573 1107 70-71 -0.1251971004976653 1107 72-73 -0.06416645474423888 1107 74-75 -0.23900470060992518 1107 76-77 -0.33749734243901486 1107 78-79 -0.3241295919723193 1107 80-81 -0.38426691628012577 1107 82-83 -0.1311257834810462 1107 84-85 -0.13146765229922863 1107 86-87 -0.018199634781613128 1107 88-89 -0.015481238331023661 1107 90-91 0.16154258319370385 1107 92-93 0.24021596345141916 1107 94-95 0.05562556712412814 1107 96-97 0.2829423239140283 1107 98-99 -0.04417975310256139 1107 100-101 0.09898678128406502 1108 1 -0.09945391727874764 1108 2 -0.1666365972364794 1108 3 -0.14019532473734486 1108 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-0.2992958282413767 2316 60-61 -0.2096446143948043 2316 62-63 -0.4137456342161201 2316 64-65 -0.5208429645323207 2316 66-67 -0.5594855189806722 2316 68-69 -0.1380083080358645 2316 70-71 -0.2518165112711941 2316 72-73 -0.48205606077993224 2316 74-75 -0.7851345858081302 2316 76-77 -0.45734321418051493 2316 78-79 -0.3763377336292173 2316 80-81 -0.43035890347643146 2316 82-83 -0.35229706495825397 2316 84-85 -0.48529505896811287 2316 86-87 -0.8793690505110305 2316 88-89 -0.6912456118770507 2316 90-91 -0.7360395242850295 2316 92-93 -0.6862466078988554 2316 94-95 -0.8103875047712954 2316 96-97 -0.6830157958786423 2316 98-99 -0.7004077066687628 2316 100-101 -1.0880158074409891 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3299.0 3 604.0 4 531.0 5 619.0 6 699.0 7 802.0 8 996.0 9 1223.0 10 1613.0 11 2066.0 12 2766.0 13 3397.0 14 4249.0 15 5181.0 16 6627.0 17 8550.0 18 7104.0 19 5194.0 20 4960.0 21 5206.0 22 5702.0 23 6455.0 24 7182.0 25 8270.0 26 9479.0 27 11384.0 28 13668.0 29 16633.0 30 20255.0 31 25636.0 32 32494.0 33 43172.0 34 58629.0 35 84248.0 36 127026.0 37 190117.0 38 172018.0 39 24067.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33863775769888 19.92149944969135 15.063774899835124 22.676087892774643 2 14.858330805370807 20.962082736679815 40.54395229816258 23.635634159786804 3 18.81765268098978 25.07353109073927 29.996226944401254 26.112589283869696 4 12.1485337882727 17.3641352509506 38.52229518214428 31.965035778632423 5 13.148378876139658 38.27259147156863 34.6357988854026 13.943230766889108 6 30.855724679885604 37.60116310404479 17.87794361814025 13.66516859792936 7 27.805397534325472 33.33909273174787 21.1128679087407 17.74264182518596 8 25.73835352515656 35.63211146451481 20.136657477204736 18.4928775331239 9 25.872234537314732 17.511300908419177 20.703590211674708 35.91287434259138 10-11 24.06712119763186 27.652529406139426 27.793854921018546 20.48649447521017 12-13 25.26375672602065 24.906285334870656 28.026648163714178 21.803309775394514 14-15 22.185817227246847 26.5624932091495 26.45758815067213 24.794101412931518 16-17 21.348053318380803 29.82997471870278 27.03229735978062 21.789674603135794 18-19 21.271695958598876 28.67096244247505 29.268825347431417 20.788516251494656 20-21 22.120276340466404 27.650943047698938 29.280169594290644 20.94861101754401 22-23 21.688812780674695 27.556998762396407 29.224884198768482 21.529304258160415 24-25 21.479907302079837 27.91514424565601 29.309198410706543 21.29575004155761 26-27 21.318443099457777 28.101413684816745 29.145595410142455 21.434547805583023 28-29 21.137406151985566 28.49198281655691 29.142365380154068 21.228245651303453 30-31 21.453689154327467 28.089852842416853 28.968538573716803 21.48791942953888 32-33 21.235804445970636 28.067546413844852 29.023669716097068 21.67297942408744 34-35 21.79916452110456 28.20874041967358 28.871940502689036 21.12015455653282 36-37 21.77530814832844 27.974457154778253 28.88282105665757 21.367413640235743 38-39 21.39383588433157 28.38382093469966 28.852789583930882 21.36955359703789 40-41 21.786894053265353 28.27457181674425 28.682805146944983 21.255728983045415 42-43 21.369995621559312 28.350566426812136 28.50516992804364 21.774268023584913 44-45 21.63051728362664 28.371248486191952 28.524788963340992 21.473445266840418 46-47 21.821307055395867 28.588013579373 28.037472028805915 21.553207336425217 48-49 21.716743933599517 28.549230708213514 27.982129633503188 21.751895724683784 50-51 21.76207552295572 28.731757674544962 27.934202662320022 21.571964140179297 52-53 21.800017713607907 28.7731479091694 27.656266895168585 21.770567482054105 54-55 21.704560767488665 28.704319514538206 27.825756686618934 21.76536303135419 56-57 21.801236972730326 28.810024845921607 27.676694482076066 21.712043699272005 58-59 21.915820186687263 28.586147600906823 27.76943156303058 21.728600649375338 60-61 21.620461856180807 28.72375212248086 28.0304898517441 21.625296169594233 62-63 21.370105437434173 28.63285491502702 28.340187409322233 21.656852238216576 64-65 21.41315305763289 28.682065276431914 28.05698108452216 21.847800581413036 66-67 21.42550994299894 28.63628239887921 27.832574532428893 22.105633125692957 68-69 21.497846554296764 28.44058374701182 28.018857112128554 22.042712586562867 70-71 21.581828344649153 28.17728836410668 28.064193536646908 22.176689754597263 72-73 21.34348142594826 28.31518845681628 28.20811195497463 22.133218162260828 74-75 21.235168252033382 28.691851101010972 27.849165000361236 22.223815646594407 76-77 21.390406288963078 28.39334269578988 28.020003422638855 22.196247592608188 78-79 21.412844066601437 28.360861196659386 27.84557957909342 22.380715157645753 80-81 21.39861834007433 28.34722272014705 27.724563193179264 22.529595746599355 82-83 21.414811329877452 28.239781477525717 28.01503319885741 22.33037399373943 84-85 21.40047745401314 28.21324106539056 28.076685958560905 22.3095955220354 86-87 21.324495181313207 28.170876250300115 27.946645170612257 22.55798339777442 88-89 21.257538702566336 27.904906145801306 27.893661242675876 22.943893908956483 90-91 21.2239073361758 27.896002533555762 28.307651023335982 22.572439106932457 92-93 21.232341285470216 28.014428470153003 28.212439666341627 22.540790578035153 94-95 20.930413213981893 28.221060191065156 28.005777803369984 22.842748791582963 96-97 20.991589545027143 28.27836905726095 27.93028279835713 22.79975859935478 98-99 21.199318772595536 28.54815446586937 27.52653065659131 22.72599610494379 100-101 21.320157862319117 28.09035695003742 27.500871762393963 23.0886134252495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1132.0 1 630.5 2 125.0 3 103.0 4 84.0 5 87.0 6 123.0 7 196.0 8 304.0 9 391.5 10 445.0 11 478.0 12 501.0 13 565.0 14 675.0 15 751.5 16 833.5 17 980.0 18 1063.0 19 1167.0 20 1364.0 21 1560.5 22 1865.0 23 2300.5 24 2767.0 25 3517.5 26 4856.5 27 6536.5 28 8093.0 29 9973.0 30 12075.5 31 14351.0 32 17466.0 33 20124.5 34 22510.0 35 24896.0 36 27939.5 37 30851.5 38 31696.0 39 32366.0 40 33000.0 41 33792.5 42 34415.0 43 34924.5 44 35831.0 45 36248.0 46 36176.0 47 36727.5 48 37563.5 49 36402.5 50 33792.0 51 31291.5 52 29228.5 53 27701.5 54 26782.5 55 24353.0 56 20526.5 57 17506.0 58 15470.0 59 13164.5 60 10263.0 61 7943.0 62 6112.0 63 4432.5 64 3117.5 65 2256.5 66 1612.5 67 1173.0 68 861.0 69 628.5 70 446.0 71 310.0 72 225.0 73 187.5 74 154.5 75 101.0 76 70.5 77 48.5 78 30.0 79 17.5 80 15.0 81 11.5 82 9.0 83 6.5 84 1.5 85 2.0 86 2.5 87 2.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2862955524419709 2 0.26146158217333026 3 0.2651487218638708 4 0.2647149407238072 5 0.25267751408704253 6 0.19823798100906168 7 0.20485314339503147 8 0.2024673471246817 9 0.19997310556931605 10-11 0.20691360381033352 12-13 0.2071847170228733 14-15 0.1917312639081078 16-17 0.20371446790236453 18-19 0.19113481484052036 20-21 0.19628596587877553 22-23 0.19487617717356884 24-25 0.1859836638022652 26-27 0.1988886527191571 28-29 0.19731619608642656 30-31 0.20452780753998376 32-33 0.20577492831766658 34-35 0.19666552437633114 36-37 0.19390016960842577 38-39 0.18435698452702673 40-41 0.1837605354594393 42-43 0.1848992109521062 44-45 0.20013577349683992 46-47 0.19059258841544086 48-49 0.19840064893658554 50-51 0.203226464119793 52-53 0.20902828686814356 54-55 0.2090825095106515 56-57 0.17904316556124775 58-59 0.16776485591959434 60-61 0.17578980701077077 62-63 0.17627781079334232 64-65 0.17438001830556413 66-67 0.18462809773956648 68-69 0.17595247493829463 70-71 0.17974805991385107 72-73 0.19021302991788525 74-75 0.18159162975912133 76-77 0.19319527525582242 78-79 0.1901588072753773 80-81 0.17752493157102517 82-83 0.19097214691299652 84-85 0.17530180322819924 86-87 0.17839249385115233 88-89 0.18511610152213803 90-91 0.18202541089918492 92-93 0.18668855815486854 94-95 0.1848992109521062 96-97 0.18029028633893054 98-99 0.18723078457994802 100-101 0.17080132390003946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 922124.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.5052861074633 #Duplication Level Percentage of deduplicated Percentage of total 1 83.83256578233126 56.5914133825901 2 10.193816637303737 13.762730172564167 3 2.6947236430943726 5.457242715228943 4 1.1031077470067314 2.978624162761946 5 0.5384906113566036 1.8175481392905177 6 0.31555594759341793 1.2781016715123223 7 0.22405021386026064 1.0587201652352665 8 0.16752517952298354 0.9047068139122525 9 0.11618892878216404 0.7059030191963694 >10 0.7382078545453866 9.377030283948972 >50 0.05201381089907135 2.4214336718173137 >100 0.022309165587026558 2.5207397353622083 >500 6.419902742428743E-4 0.22849821907578574 >1k 8.024878428035929E-4 0.8973078475038679 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2749 0.2981160885087038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1910 0.20713049438036532 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1244 0.1349059345597772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.13382148170961822 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1145 0.12416985134320331 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.0844528501589807E-4 0.0 6 0.0 0.0 0.0 1.0844528501589807E-4 0.0 7 0.0 0.0 0.0 1.0844528501589807E-4 0.0 8 0.0 0.0 0.0 1.0844528501589807E-4 0.0 9 0.0 0.0 0.0 1.0844528501589807E-4 0.0 10-11 0.0 0.0 0.0 1.0844528501589807E-4 0.0 12-13 0.0 0.0 0.0 1.0844528501589807E-4 0.0 14-15 1.0844528501589807E-4 0.0 0.0 1.0844528501589807E-4 0.0 16-17 1.0844528501589807E-4 0.0 0.0 1.0844528501589807E-4 0.0 18-19 1.0844528501589807E-4 0.0 0.0 1.0844528501589807E-4 0.0 20-21 1.0844528501589807E-4 0.0 0.0 1.0844528501589807E-4 0.0 22-23 1.0844528501589807E-4 0.0 0.0 3.253358550476942E-4 0.0 24-25 1.0844528501589807E-4 0.0 0.0 5.964490675874394E-4 0.0 26-27 1.0844528501589807E-4 0.0 0.0 8.133396376192356E-4 0.0 28-29 1.0844528501589807E-4 0.0 0.0 0.0017893472027623184 0.0 30-31 1.0844528501589807E-4 0.0 0.0 0.0035786944055246368 0.0 32-33 1.0844528501589807E-4 0.0 0.0 0.007048943526033375 0.0 34-35 1.0844528501589807E-4 0.0 0.0 0.008729845443779794 0.0 36-37 1.0844528501589807E-4 0.0 0.0 0.011766313424224942 0.0 38-39 1.0844528501589807E-4 0.0 0.0 0.019032147520290116 0.0 40-41 1.0844528501589807E-4 0.0 0.0 0.034160264780007894 0.0 42-43 1.0844528501589807E-4 0.0 0.0 0.06409116344439576 0.0 44-45 1.0844528501589807E-4 0.0 0.0 0.08849135257297283 0.0 46-47 1.0844528501589807E-4 0.0 0.0 0.15540209342778194 0.0 48-49 1.0844528501589807E-4 0.0 0.0 0.2281146570309416 0.0 50-51 1.0844528501589807E-4 0.0 0.0 0.2782163787082865 0.0 52-53 1.0844528501589807E-4 0.0 0.0 0.3574356594124001 0.0 54-55 1.0844528501589807E-4 0.0 0.0 0.4355162646238467 0.0 56-57 1.0844528501589807E-4 0.0 0.0 0.616023441532809 0.0 58-59 1.0844528501589807E-4 0.0 0.0 0.8034711166827888 0.0 60-61 1.0844528501589807E-4 0.0 0.0 1.017216773449124 0.0 62-63 1.0844528501589807E-4 0.0 0.0 1.2418069587170488 0.0 64-65 1.0844528501589807E-4 0.0 0.0 1.4847243971526605 0.0 66-67 1.0844528501589807E-4 0.0 0.0 1.8454134151155377 0.0 68-69 1.0844528501589807E-4 0.0 0.0 2.3180179672148213 0.0 70-71 1.0844528501589807E-4 0.0 0.0 2.657885490454646 0.0 72-73 1.0844528501589807E-4 0.0 0.0 2.990216066385866 0.0 74-75 1.0844528501589807E-4 0.0 0.0 3.429961697125332 0.0 76-77 1.0844528501589807E-4 0.0 0.0 3.9405763216226886 0.0 78-79 1.0844528501589807E-4 0.0 0.0 4.458511002858618 0.0 80-81 1.0844528501589807E-4 0.0 0.0 5.225273390563525 0.0 82-83 1.0844528501589807E-4 0.0 0.0 6.053361586944923 0.0 84-85 1.0844528501589807E-4 0.0 0.0 6.972977603879738 0.0 86-87 1.0844528501589807E-4 0.0 0.0 7.808060521144661 0.0 88-89 1.0844528501589807E-4 0.0 0.0 9.049704811934188 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4445 0.0 29.416492 1 GGTATCA 3080 0.0 26.552637 1 ATACCGT 95 0.0012250161 25.004734 6 TACATGG 3555 0.0 25.004253 2 GTACATG 3645 0.0 24.523943 1 ATCAACG 5240 0.0 24.493135 3 TCAACGC 5250 0.0 24.44515 4 CAACGCA 5255 0.0 24.419237 5 TATCAAC 5400 0.0 23.943468 2 AACGCAG 5400 0.0 23.754496 6 ACATGGG 3550 0.0 23.43266 3 GAGTACT 2660 0.0 22.503649 12-13 AGAGTAC 4335 0.0 21.644222 10-11 ACGCAGA 6110 0.0 20.762014 7 CGCAGAG 6110 0.0 20.762014 8 AGTACTT 2875 0.0 20.490278 12-13 GTACTTT 2920 0.0 20.336096 14-15 GTATCGG 110 1.3446079E-6 19.433386 44-45 GCAGAGT 6415 0.0 19.32841 9 CATGGGG 1900 0.0 19.012896 4 >>END_MODULE