FastQCFastQC Report
Thu 26 May 2016
SRR937213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922124
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40420.43833584203426007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.3335776967089025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.21157675106601714No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10570.11462666626180426No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG500.001615165638.002324
GGTATCA28050.037.6203581
GTATCAA46000.037.613731
ATCGGAC1308.283678E-932.884848
GTACGCT600.0039493731.6685983
GATCGGA1351.1965312E-831.6668857
ATACGGT600.00395146731.6651656
ATCAACG57100.030.1156753
TCAACGC57250.030.036774
CAACGCA57750.029.776715
TATCAAC58250.029.6842162
AACGCAG58900.029.2728086
GGATCGG1606.241862E-826.7174846
GGACCGA1806.697519E-926.3876386
ACGCAGA66400.025.6816527
CGCAGAG65900.025.6602698
TCGGACC1701.1222437E-725.147239
TATTCCG1351.3417184E-524.6311345
ATTGGCG1551.410046E-624.5162967
AGGATCG1751.4846046E-724.4300635
Produced by FastQC (version 0.11.5)