FastQCFastQC Report
Thu 26 May 2016
SRR937212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128288
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.27998170679826423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22450.19897402081737997No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15760.13968064891233445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.1258543917864942No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14120.12514535295952806No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG600.003951027531.6664898
GTATCAA58500.030.7829021
CCGTCGT700.0083917327.1427049
GGACCGA1606.239134E-826.7197886
TCAACGC67100.026.4091634
ATCAACG67900.026.2379443
CAACGCA67550.026.2332325
TATCAAC68750.025.91472
AACGCAG69600.025.3889166
GGTATCA39300.023.9386061
TACATGG41850.023.272662
GTACATG42900.023.1480921
AGTACTT34350.023.09302912-13
ACATGGG40950.022.8549693
CGCAGAG75950.022.827398
ACGCAGA77400.022.6452227
AGAGTAC52750.022.60126710-11
GAGTACT32400.022.28382512-13
GGCGACA2005.383881E-721.375836
GTACTTT34050.020.9245314-15
Produced by FastQC (version 0.11.5)