##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937212_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1128288 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825822839558693 33.0 31.0 34.0 30.0 34.0 2 31.93000811849457 33.0 31.0 34.0 30.0 34.0 3 31.983803780595025 34.0 31.0 34.0 30.0 34.0 4 35.58044045491931 37.0 35.0 37.0 33.0 37.0 5 35.598914461555914 37.0 35.0 37.0 33.0 37.0 6 35.623800838083895 37.0 35.0 37.0 33.0 37.0 7 35.507450225474344 37.0 35.0 37.0 33.0 37.0 8 35.51884447942369 37.0 35.0 37.0 33.0 37.0 9 37.16505626223092 39.0 37.0 39.0 34.0 39.0 10-11 37.055778755069625 39.0 37.0 39.0 33.0 39.0 12-13 36.92331434881874 39.0 37.0 39.0 33.0 39.0 14-15 38.13318230806318 40.0 38.0 41.0 33.0 41.0 16-17 38.07492812118892 40.0 38.0 41.0 33.0 41.0 18-19 38.04636449204459 40.0 38.0 41.0 33.0 41.0 20-21 37.92945861340367 40.0 38.0 41.0 33.0 41.0 22-23 37.78067966689356 40.0 37.5 41.0 33.0 41.0 24-25 37.727291258969345 40.0 37.5 41.0 32.5 41.0 26-27 37.73768488187413 40.0 37.5 41.0 33.0 41.0 28-29 37.675515914376476 40.0 37.0 41.0 33.0 41.0 30-31 37.483654439292096 40.0 37.0 41.0 32.0 41.0 32-33 37.27161770753567 40.0 37.0 41.0 31.5 41.0 34-35 37.34229203891205 40.0 37.0 41.0 32.0 41.0 36-37 37.25070505048356 40.0 37.0 41.0 31.5 41.0 38-39 37.14904483607022 40.0 37.0 41.0 31.0 41.0 40-41 36.96335775972092 40.0 36.5 41.0 31.0 41.0 42-43 36.73095122876428 39.0 36.0 41.0 30.0 41.0 44-45 36.52741587254318 39.0 36.0 41.0 30.0 41.0 46-47 36.23911536770754 39.0 36.0 41.0 30.0 41.0 48-49 35.950728891914125 39.0 35.0 41.0 28.5 41.0 50-51 34.988056684109026 38.0 34.0 40.0 27.0 40.5 52-53 35.10647015655577 38.5 34.5 39.5 27.5 40.5 54-55 35.50214883079498 39.0 35.0 40.0 28.0 41.0 56-57 35.30699785870274 39.0 35.0 40.0 26.5 41.0 58-59 35.0567470362177 38.5 35.0 40.0 26.0 41.0 60-61 34.75685507600896 38.0 34.0 40.0 26.0 41.0 62-63 34.4769682031538 38.0 34.0 40.0 26.0 41.0 64-65 34.06932228296321 37.5 34.0 40.0 24.0 41.0 66-67 33.72327056567117 37.0 33.5 39.5 23.0 41.0 68-69 33.2552863276043 36.0 33.0 39.0 22.0 41.0 70-71 32.794209014010605 36.0 32.0 39.0 21.5 40.0 72-73 32.33763055177401 35.0 32.0 38.5 20.0 40.0 74-75 31.863518002495816 35.0 31.0 37.5 18.5 39.5 76-77 31.38860601194021 35.0 31.0 37.0 17.0 39.0 78-79 30.87745947843104 35.0 31.0 36.0 12.0 39.0 80-81 30.397753499106614 34.0 30.0 36.0 7.5 37.5 82-83 29.92802059403273 34.0 30.0 35.5 6.0 37.0 84-85 29.41244611304915 34.0 29.0 35.0 2.0 37.0 86-87 28.981125829575426 34.0 29.0 35.0 2.0 36.0 88-89 28.5388318408066 34.0 29.0 35.0 2.0 36.0 90-91 28.054949179642076 34.0 27.5 35.0 2.0 35.5 92-93 27.568239226155022 33.0 27.0 35.0 2.0 35.0 94-95 27.054482986613348 33.0 25.5 35.0 2.0 35.0 96-97 26.36530610978757 33.0 25.0 35.0 2.0 35.0 98-99 25.481215345727335 32.0 22.0 34.5 2.0 35.0 100-101 22.964071673189824 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.22062098259907614 1101 2 -0.0922518044073577 1101 3 -0.24643737187514603 1101 4 -0.21051090128509742 1101 5 -0.27755114101935163 1101 6 -0.18622362109584856 1101 7 -0.19500912205000276 1101 8 -0.26557456391508083 1101 9 -0.271237444803333 1101 10-11 -0.4657340459039716 1101 12-13 -0.33617114145483384 1101 14-15 -0.5438855088140926 1101 16-17 -0.5834161459043443 1101 18-19 -0.33364979341062195 1101 20-21 -0.2107104712036758 1101 22-23 -0.5567260353865819 1101 24-25 -0.4149560633042668 1101 26-27 -0.5361025450772985 1101 28-29 -0.5801402760809893 1101 30-31 -0.3477412555944781 1101 32-33 -0.5816324160607991 1101 34-35 -0.5214780864924364 1101 36-37 -0.3607430876723754 1101 38-39 -0.39413638505813964 1101 40-41 -0.5519639826168898 1101 42-43 -0.3939910876237107 1101 44-45 -0.4622539281862501 1101 46-47 -0.4497925228958195 1101 48-49 -0.5007947878446402 1101 50-51 -0.3840798041433544 1101 52-53 -0.3556861519383787 1101 54-55 -0.503407826792909 1101 56-57 -0.4434564612121292 1101 58-59 -0.5638573924503163 1101 60-61 -0.46259460777970673 1101 62-63 -0.6376115127077853 1101 64-65 -0.5361882600483199 1101 66-67 -0.4624148397532153 1101 68-69 -0.46348902292914573 1101 70-71 -0.43486593394169404 1101 72-73 -0.32947069085818725 1101 74-75 -0.40389607608138434 1101 76-77 -0.4351812515254032 1101 78-79 -0.5915813710735982 1101 80-81 -0.33617946748656635 1101 82-83 -0.35220200380782885 1101 84-85 -0.5811706314619087 1101 86-87 -0.6110813390623555 1101 88-89 -0.6317495523219065 1101 90-91 -0.6843042328601534 1101 92-93 -0.5651447624881705 1101 94-95 -0.6815732979612719 1101 96-97 -0.5558388422875176 1101 98-99 -0.4282174242333845 1101 100-101 -0.08949645269122186 1102 1 -0.17829411279298313 1102 2 -0.12471717836857721 1102 3 -0.11303016965907986 1102 4 -0.2454139483765161 1102 5 -0.21201097481436904 1102 6 -0.30040644658061666 1102 7 -0.13971826332424087 1102 8 -0.20449423150510881 1102 9 -0.1433427079612315 1102 10-11 -0.1524237965964943 1102 12-13 -0.19680826056841028 1102 14-15 -0.14061681075315136 1102 16-17 -0.12258512097358931 1102 18-19 -0.17421766044663656 1102 20-21 -0.2476495293754155 1102 22-23 -0.3006872542231491 1102 24-25 -0.20125800236242952 1102 26-27 -0.25521611848450476 1102 28-29 -0.3171208854992713 1102 30-31 -0.2461069065640089 1102 32-33 -0.22193712520207498 1102 34-35 -0.13398501170019017 1102 36-37 -0.12293145332334632 1102 38-39 -0.0959646399057803 1102 40-41 -0.1764792180739505 1102 42-43 -0.15572238956276152 1102 44-45 -0.19717082569317057 1102 46-47 -0.270360389931831 1102 48-49 -0.392927751833561 1102 50-51 -0.3529717709023572 1102 52-53 -0.29696039016553044 1102 54-55 -0.2059840040782248 1102 56-57 -0.24155895428692276 1102 58-59 -0.42905129826748833 1102 60-61 -0.23632036955255842 1102 62-63 -0.14320708057481824 1102 64-65 -0.3518669304084341 1102 66-67 -0.36927079543188057 1102 68-69 -0.36044193151639803 1102 70-71 -0.43773296995277633 1102 72-73 -0.30080033074738566 1102 74-75 -0.5039791785744576 1102 76-77 -0.29169094681626007 1102 78-79 -0.4683819250902168 1102 80-81 -0.5337140935253473 1102 82-83 -0.4922851063009013 1102 84-85 -0.589134620381575 1102 86-87 -0.5298348016662331 1102 88-89 -0.7074559235130415 1102 90-91 -0.5068665597299606 1102 92-93 -0.4079009951751509 1102 94-95 -0.3454791151357881 1102 96-97 -0.28305490876951467 1102 98-99 -0.29510108074307695 1102 100-101 0.026237619330935047 1103 1 0.02766018543729487 1103 2 -0.10800280982788735 1103 3 -0.09136830385626027 1103 4 -0.003971296459084783 1103 5 -0.07735180547118148 1103 6 -0.04101991415407724 1103 7 0.025840676865897194 1103 8 0.032965390622429425 1103 9 0.0716948860237352 1103 10-11 0.08581255315181124 1103 12-13 0.15382536143027892 1103 14-15 0.005217481219126796 1103 16-17 0.04935881475311277 1103 18-19 -0.020457801803694053 1103 20-21 0.13750337737125307 1103 22-23 -0.031429654900485104 1103 24-25 -0.059447233089187534 1103 26-27 -0.2809880145649757 1103 28-29 -0.24963669153824242 1103 30-31 -0.42406403929792447 1103 32-33 -0.43357331481931993 1103 34-35 -0.14098936468791834 1103 36-37 -0.17522988606370404 1103 38-39 -0.5795079535141596 1103 40-41 -0.24071201548976973 1103 42-43 -0.24734219795767842 1103 44-45 -0.4950734770584191 1103 46-47 -0.34209449876573217 1103 48-49 -0.34871225593346367 1103 50-51 -0.324791467021484 1103 52-53 -0.16722663104311408 1103 54-55 -0.06799429295831771 1103 56-57 -0.1549418607575035 1103 58-59 -0.38632023560471396 1103 60-61 -0.1615253649050814 1103 62-63 -0.005032390483620475 1103 64-65 -0.07873765679600808 1103 66-67 -0.11996423650089127 1103 68-69 0.04614552856587295 1103 70-71 -0.004487371255912365 1103 72-73 -0.0361589230666155 1103 74-75 -0.025345630199236524 1103 76-77 -0.04759600935019748 1103 78-79 -0.14798546269800994 1103 80-81 -0.22503940803561306 1103 82-83 -0.11692136033869005 1103 84-85 -0.40103861537517815 1103 86-87 -0.19169508272383595 1103 88-89 -0.09771737886500986 1103 90-91 -0.06579662663529362 1103 92-93 -0.00986674185519476 1103 94-95 -0.44860075031308355 1103 96-97 -0.5291339987834078 1103 98-99 -0.17197594845090336 1103 100-101 0.18905224463347992 1104 1 0.05236147354127496 1104 2 0.2017832833119364 1104 3 0.05180824215993951 1104 4 0.12001541450437259 1104 5 0.1719225431911724 1104 6 0.08360094030766163 1104 7 -0.02939508696228188 1104 8 0.005478067663872821 1104 9 0.11911343238964633 1104 10-11 0.06873963830655327 1104 12-13 0.2871621918784939 1104 14-15 0.13330747364204143 1104 16-17 0.06991718742899167 1104 18-19 0.23328003115077678 1104 20-21 0.1676228621296616 1104 22-23 0.16941431549060582 1104 24-25 0.1528509542395966 1104 26-27 0.08635359527357167 1104 28-29 0.27898253093655256 1104 30-31 0.1541885689669229 1104 32-33 0.12450793481639266 1104 34-35 0.2428201591215995 1104 36-37 0.3120639298714849 1104 38-39 0.18340747459098594 1104 40-41 0.42049215773399595 1104 42-43 0.41785101763945676 1104 44-45 0.5797636156734072 1104 46-47 0.508716249034002 1104 48-49 0.39885433496237255 1104 50-51 0.13890265199700025 1104 52-53 0.37098051472828075 1104 54-55 0.3104518223299024 1104 56-57 0.31066634580541574 1104 58-59 0.4825461163216147 1104 60-61 0.3813527606413416 1104 62-63 0.3736165574676562 1104 64-65 0.4669696346885175 1104 66-67 0.33977814270293294 1104 68-69 0.4652829068954176 1104 70-71 0.22697617132146064 1104 72-73 0.385792467036552 1104 74-75 0.5236477835677391 1104 76-77 0.3311345379482802 1104 78-79 0.40201512015446994 1104 80-81 0.52610123426782 1104 82-83 0.7553418558412979 1104 84-85 0.6175135790644077 1104 86-87 0.6420765556744854 1104 88-89 0.4852679915377429 1104 90-91 0.6419238373152822 1104 92-93 0.7177499743539322 1104 94-95 0.7396547722141662 1104 96-97 0.7731962454317838 1104 98-99 0.7545018740122309 1104 100-101 0.8799772315193017 1105 1 -0.18317149295317492 1105 2 -0.25141675589221535 1105 3 -0.29656884581138243 1105 4 -0.1586438033727191 1105 5 -0.24245138044473435 1105 6 -0.15750685711886803 1105 7 -0.19230739566700095 1105 8 -0.22440389982468645 1105 9 -0.1798613789403518 1105 10-11 -0.29151070823017733 1105 12-13 -0.2525667393882216 1105 14-15 -0.19022230051401579 1105 16-17 -0.342834804282127 1105 18-19 -0.29859770788955586 1105 20-21 -0.17179523618876402 1105 22-23 -0.07684994852137805 1105 24-25 -0.2135944889773853 1105 26-27 -0.16438414292667147 1105 28-29 -0.33803291270047 1105 30-31 -0.25416667668911685 1105 32-33 -0.16770163939847293 1105 34-35 -0.20268130403349716 1105 36-37 -0.12106778208998037 1105 38-39 -0.045899311928387476 1105 40-41 -0.12975412837233335 1105 42-43 -0.10417695732589749 1105 44-45 0.0054053736997587976 1105 46-47 -0.025034048497531103 1105 48-49 0.05866167454037452 1105 50-51 -0.20116259491918242 1105 52-53 -0.24224472714735157 1105 54-55 0.2121334005771942 1105 56-57 -0.06048947671177274 1105 58-59 0.06415768714812486 1105 60-61 0.27139098469309886 1105 62-63 0.19262161200343542 1105 64-65 0.16341605800938908 1105 66-67 0.04653105851200934 1105 68-69 0.037787912426004766 1105 70-71 -0.08548738981601645 1105 72-73 0.18866893194182666 1105 74-75 0.1884166890717225 1105 76-77 0.027405382657821775 1105 78-79 -0.015371642842406885 1105 80-81 0.06229703102227546 1105 82-83 0.1391412985822278 1105 84-85 -0.06197144634828433 1105 86-87 0.08178252450718304 1105 88-89 0.08346824076416226 1105 90-91 0.1615219947199691 1105 92-93 0.300061059328943 1105 94-95 0.1177256477269708 1105 96-97 0.22396646707527523 1105 98-99 0.2112661310234749 1105 100-101 0.392595369041878 1106 1 -0.3649434492133743 1106 2 -0.3331571461198486 1106 3 -0.4571856043967486 1106 4 -0.02815622728667222 1106 5 -0.11755114101935504 1106 6 -0.40392589917911437 1106 7 -0.5421379515865254 1106 8 -0.5449539826110694 1106 9 -0.7367539412683755 1106 10-11 -1.0597963004208637 1106 12-13 -0.6782331995852431 1106 14-15 -0.799613120754394 1106 16-17 -0.5979006313717434 1106 18-19 -1.0708532498128207 1106 20-21 -0.6671902433953463 1106 22-23 -0.7193173551037901 1106 24-25 -0.6328930232414152 1106 26-27 -0.8081223801126498 1106 28-29 -0.722942711273447 1106 30-31 -0.7018005643140341 1106 32-33 -0.7216903500749439 1106 34-35 -0.46920098515700914 1106 36-37 -0.684815161513697 1106 38-39 -0.48349125544305593 1106 40-41 -0.3787658286498825 1106 42-43 -0.33676799257264634 1106 44-45 -0.09238157940384184 1106 46-47 -0.04305646633650895 1106 48-49 0.13814963477908293 1106 50-51 0.003810023036535881 1106 52-53 0.2184399281873084 1106 54-55 -0.17986796033571295 1106 56-57 -0.06855072672666296 1106 58-59 0.0994978707075802 1106 60-61 -0.12781063291717487 1106 62-63 -0.34626528332836415 1106 64-65 -0.28189132367754155 1106 66-67 -0.11761417203914704 1106 68-69 -0.2840114109888461 1106 70-71 -0.024726499534786228 1106 72-73 -0.06130985032401526 1106 74-75 0.12126888856904827 1106 76-77 0.22426984823893648 1106 78-79 0.14971870748099647 1106 80-81 -0.17898583040879856 1106 82-83 -0.20690840600735427 1106 84-85 -0.25267495196465717 1106 86-87 -0.3009016454252773 1106 88-89 0.25069153173151193 1106 90-91 0.3621676838719736 1106 92-93 0.4720910976846504 1106 94-95 0.4241238740732918 1106 96-97 0.34419061568577547 1106 98-99 0.28399977922302 1106 100-101 0.6567643485656518 1107 1 0.012668491085133127 1107 2 0.021047790734343153 1107 3 -0.019585145154497496 1107 4 0.04713079911975626 1107 5 0.19687193590372232 1107 6 0.11417111574625949 1107 7 0.18568925851680262 1107 8 0.1677756385140725 1107 9 0.15012895195779663 1107 10-11 0.1855969257559451 1107 12-13 0.11145537676378581 1107 14-15 0.4024606450320576 1107 16-17 0.3158551091563879 1107 18-19 0.10983704869464361 1107 20-21 0.1750385166505808 1107 22-23 0.29392173789276654 1107 24-25 0.19072207434755484 1107 26-27 0.2545857140320109 1107 28-29 0.4078911004372259 1107 30-31 0.3791767605998544 1107 32-33 0.34483519527523043 1107 34-35 0.33249964630108764 1107 36-37 0.33811824836001847 1107 38-39 0.2695225218244488 1107 40-41 0.4027424951159091 1107 42-43 0.2990959569107119 1107 44-45 0.13631388557893587 1107 46-47 0.28985828682077397 1107 48-49 0.18670015142661356 1107 50-51 0.24161857642344842 1107 52-53 0.08874704644218667 1107 54-55 0.0817996630856328 1107 56-57 0.11246965214819937 1107 58-59 0.09709402455374061 1107 60-61 0.4405734084146289 1107 62-63 0.6690332241343242 1107 64-65 0.6897935213281983 1107 66-67 0.6009707877771575 1107 68-69 0.6504532847631666 1107 70-71 0.7177605842769239 1107 72-73 0.8834088637976834 1107 74-75 0.8505168793842017 1107 76-77 0.6586699630494941 1107 78-79 0.9426291552421908 1107 80-81 0.7298832855498709 1107 82-83 0.8487747168399444 1107 84-85 0.7377311904004422 1107 86-87 1.0004976083060662 1107 88-89 0.881798018528297 1107 90-91 1.1476441477066466 1107 92-93 1.0501335775927991 1107 94-95 1.1043477546703073 1107 96-97 1.16541621844123 1107 98-99 1.5010993773860513 1107 100-101 1.8103163003452138 1108 1 -0.21088258946496197 1108 2 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1.5933547511632078 2312 88-89 1.4960019693393924 2312 90-91 1.545704718088647 2312 92-93 1.7484230857037382 2312 94-95 2.111065441745133 2312 96-97 1.4232785941607453 2312 98-99 1.2957585872238297 2312 100-101 1.095641391920303 2313 1 0.6085962531247979 2313 2 0.6506675335977086 2313 3 0.5833702917598593 2313 4 0.6294771708783102 2313 5 0.4957633065727123 2313 6 0.6009629004488062 2313 7 0.3605775784122045 2313 8 0.776195228838759 2313 9 0.8145520288808754 2313 10-11 0.7423671916090697 2313 12-13 0.8874564118476798 2313 14-15 1.113734887786471 2313 16-17 1.0087293737020389 2313 18-19 0.9146706180997413 2313 20-21 1.1022394318826585 2313 22-23 0.9915682839812447 2313 24-25 1.1282547612074367 2313 26-27 1.221986019109373 2313 28-29 1.3783135781012064 2313 30-31 1.3117533023300822 2313 32-33 1.253755984118122 2313 34-35 1.5311676567608785 2313 36-37 1.5306315954944694 2313 38-39 1.3786353459691512 2313 40-41 1.6678138614266516 2313 42-43 1.6230962837793115 2313 44-45 1.5713773525652144 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0.44544200576955006 2314 8 0.30064455531155687 2314 9 0.32475611051352615 2314 10-11 0.5799748692410276 2314 12-13 0.6873089874388256 2314 14-15 0.7697879605736588 2314 16-17 0.7836730055457153 2314 18-19 0.8222094976742298 2314 20-21 0.6201327081733439 2314 22-23 0.6744554898550916 2314 24-25 0.8018484479739314 2314 26-27 0.8854334377368787 2314 28-29 0.8542313673132114 2314 30-31 1.0714746412905924 2314 32-33 1.0324921811465018 2314 34-35 0.9988065536794224 2314 36-37 1.0954867726928228 2314 38-39 1.3829742486690364 2314 40-41 1.2425580367171634 2314 42-43 1.299311812483701 2314 44-45 1.3508448902885135 2314 46-47 1.1115125254393021 2314 48-49 1.1867884559792046 2314 50-51 1.369228466533336 2314 52-53 1.2971065440873986 2314 54-55 1.074862849898821 2314 56-57 0.929728286371116 2314 58-59 1.0646764421361539 2314 60-61 1.0975396564523052 2314 62-63 1.070691387399627 2314 64-65 1.2298052952685339 2314 66-67 1.4702113686248808 2314 68-69 1.353857915846369 2314 70-71 1.3711652153601293 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0.8978908152368064 2315 32-33 1.0808614353902541 2315 34-35 0.6946581653521307 2315 36-37 0.7307719147870415 2315 38-39 0.7790165908395537 2315 40-41 0.751798929335898 2315 42-43 0.9957260791249425 2315 44-45 0.9953137058521193 2315 46-47 0.5068110186683725 2315 48-49 0.7481083110240263 2315 50-51 0.9649561033824625 2315 52-53 0.9321151269598005 2315 54-55 1.0230604466945508 2315 56-57 1.0337717729246165 2315 58-59 1.114275908090761 2315 60-61 1.0144860611824171 2315 62-63 0.7909374297417955 2315 64-65 0.6448348584956989 2315 66-67 0.7914587460805294 2315 68-69 0.943987612584877 2315 70-71 0.8000799588276095 2315 72-73 0.6798996977301073 2315 74-75 0.9353119908148244 2315 76-77 0.994233406615276 2315 78-79 1.2124889683263014 2315 80-81 1.093515564485891 2315 82-83 1.220247578090845 2315 84-85 1.3617831314500428 2315 86-87 1.331806019521018 2315 88-89 1.3252155239201002 2315 90-91 1.368236347562867 2315 92-93 1.2307455473986941 2315 94-95 1.2075019602777814 2315 96-97 1.5971739466942836 2315 98-99 1.6287009230366607 2315 100-101 1.3778621306830985 2316 1 0.013726484815542506 2316 2 0.08651077602491242 2316 3 0.20265683700127468 2316 4 0.05675522641659825 2316 5 0.18965889032860872 2316 6 0.23725105305032912 2316 7 0.36881862914616903 2316 8 0.13567110511643676 2316 9 0.23461472480563828 2316 10-11 -0.2778269348661908 2316 12-13 0.05143156436928109 2316 14-15 0.36902985168747193 2316 16-17 0.25377408673053736 2316 18-19 0.3236105596664274 2316 20-21 0.4405376726669772 2316 22-23 0.4089446460191226 2316 24-25 0.33203205746788456 2316 26-27 0.46484614325796514 2316 28-29 0.5116845053082244 2316 30-31 0.6129978963606462 2316 32-33 0.8511922003391348 2316 34-35 0.6138023952762524 2316 36-37 0.42986077307667614 2316 38-39 0.37418402411526586 2316 40-41 0.44306984018462714 2316 42-43 0.5006904830742016 2316 44-45 0.6646219998784275 2316 46-47 0.6547983037004457 2316 48-49 0.5359631728879108 2316 50-51 0.4740426178992152 2316 52-53 0.5409233184460405 2316 54-55 0.5206756577806004 2316 56-57 0.5000404864862702 2316 58-59 0.596852886381555 2316 60-61 0.6149313780312227 2316 62-63 0.5739509357330661 2316 64-65 0.7401341289963881 2316 66-67 0.8003182075987141 2316 68-69 0.7553197545003272 2316 70-71 0.8989593424153455 2316 72-73 0.865719871755239 2316 74-75 1.0345414754777664 2316 76-77 0.9724495024755875 2316 78-79 1.232205957750029 2316 80-81 1.0304168037558057 2316 82-83 0.8155805403268026 2316 84-85 0.6995544964047831 2316 86-87 0.8446982352653123 2316 88-89 0.8176597860711006 2316 90-91 0.9257774368313143 2316 92-93 0.9728382436164331 2316 94-95 1.3254684443254803 2316 96-97 1.3695818803655087 2316 98-99 1.5162232621220006 2316 100-101 0.9650622009962859 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4160.0 3 915.0 4 625.0 5 786.0 6 837.0 7 1041.0 8 1265.0 9 1527.0 10 1963.0 11 2588.0 12 3408.0 13 4291.0 14 4889.0 15 6517.0 16 8130.0 17 10163.0 18 8692.0 19 6461.0 20 6455.0 21 6826.0 22 7436.0 23 8271.0 24 9345.0 25 10872.0 26 12505.0 27 14718.0 28 17785.0 29 21296.0 30 26169.0 31 33056.0 32 41821.0 33 55120.0 34 75336.0 35 106394.0 36 159148.0 37 231353.0 38 192991.0 39 23132.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48436389481166 19.719616204690833 15.01457000710732 22.781449893390192 2 14.83714526801972 20.790602655771195 40.66705156104277 23.705200515166318 3 18.868155779563924 24.93201296682801 29.830631910830853 26.36919934277721 4 12.1666691832368 17.29443248826908 38.42767066564464 32.11122766284947 5 13.15565558453001 38.193314480200655 34.65222365120757 13.99880628406176 6 30.975787867104227 37.38719690414469 17.919767881922542 13.717247346828543 7 27.96814879767308 33.2765640644309 20.991569896829184 17.763717241066836 8 25.799394008842036 35.43875731839586 20.136281388823008 18.6255672839391 9 25.80750891803931 17.40278981851257 20.779831019143586 36.00987024430454 10-11 24.099491058206276 27.489119803423563 27.790584976578376 20.620804161791785 12-13 25.26541013366783 24.763350681620217 27.97305766776661 21.99818151694534 14-15 22.26719209319822 26.40930063585019 26.388648939368842 24.934858331582753 16-17 21.38802346375564 29.67629398454761 27.029869826438212 21.90581272525854 18-19 21.409552674461935 28.486274043020387 29.138717240711564 20.965456041806107 20-21 22.252121337013012 27.453027696183145 29.19237748740255 21.10247347940129 22-23 21.83916774917008 27.38562657518125 29.068336497574084 21.706869178074587 24-25 21.627547066751635 27.70888889382309 29.20720916755297 21.456354871872303 26-27 21.36755477191876 27.914692153614524 29.079947237754318 21.6378058367124 28-29 21.27062240866624 28.350229514167253 28.935485088796554 21.44366298836995 30-31 21.574982422368606 27.86228908507929 28.90873000891429 21.653998483637814 32-33 21.384993937348156 27.849779479366997 28.915562583000593 21.849664000284257 34-35 21.8366083270925 28.02431659546343 28.808040858134536 21.33103421930954 36-37 21.824424368766383 27.694917000562242 28.82344111254509 21.657217518126288 38-39 21.358442469701615 28.231213667470477 28.786376939030184 21.623966923797724 40-41 21.83157073870878 28.06917506663576 28.645471122136396 21.453783072519066 42-43 21.550467605994097 28.085848712207138 28.36985862124403 21.99382506055473 44-45 21.79813649836343 28.021194366232216 28.513742624990396 21.666926510413962 46-47 21.87916297056698 28.282769505078743 28.030598152934793 21.807469371419486 48-49 21.80885276835576 28.312192279058362 27.97464317252628 21.904311780059594 50-51 21.895716618425986 28.473329033427326 27.90177024642726 21.72918410171943 52-53 21.993916149127653 28.520972047784042 27.627809071752925 21.857302731335388 54-55 21.787647209330423 28.41362179373281 27.827183175670395 21.971547821266373 56-57 21.863309352517984 28.580246913580247 27.620348165911718 21.93609556799005 58-59 22.019618778328763 28.26682142400432 27.804059094332118 21.909500703334803 60-61 21.76057651321398 28.418984441602728 28.027271597044617 21.793167448138675 62-63 21.47009913300252 28.41393856640135 28.32184367402671 21.79411862656942 64-65 21.57795440750672 28.39622603809927 28.05739711362691 21.9684224407671 66-67 21.502856396899304 28.380942908471862 27.836615695841015 22.27958499878782 68-69 21.630835959063475 28.19927604446039 27.94999449395934 22.219893502516793 70-71 21.7184959940747 27.982907933654786 28.03570453264797 22.262891539622537 72-73 21.460463559014585 28.026728111008335 28.182766425208406 22.33004190476867 74-75 21.396269580616455 28.462609659445143 27.763977527251672 22.37714323268673 76-77 21.522204102225206 28.116461839641577 27.994729212632834 22.36660484550038 78-79 21.550334443853313 28.14968305327565 27.81763728362846 22.48234521924258 80-81 21.603626374699928 28.1914397592127 27.671311834297978 22.533622031789395 82-83 21.662188439023502 27.993829504082356 27.926511773720065 22.417470283174076 84-85 21.529579876412296 28.081877296843167 27.96535885371429 22.423183973030245 86-87 21.48779231390939 28.042995568108143 27.809499702468184 22.659712415514285 88-89 21.490861519086497 27.819011983626314 27.745207837272073 22.944918660015116 90-91 21.485776207595645 27.744333687882627 28.121917586163253 22.647972518358475 92-93 21.449591483930902 27.907427003758105 28.013868986216718 22.62911252609427 94-95 21.25182894202226 28.04268234468702 27.86217492567668 22.84331378761404 96-97 21.324218089058373 28.111178860615187 27.73858126025032 22.826021790076116 98-99 21.45371778786903 28.11631341112727 27.502681337226733 22.927287463776967 100-101 21.70787155441887 27.59315501562944 27.600081699346408 23.09889173060529 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1446.0 1 842.5 2 188.0 3 140.5 4 126.0 5 106.0 6 123.0 7 192.5 8 329.0 9 428.0 10 457.0 11 512.0 12 585.0 13 648.0 14 755.0 15 834.0 16 877.0 17 974.5 18 1116.5 19 1301.0 20 1465.5 21 1730.5 22 2130.5 23 2570.0 24 3200.0 25 4128.5 26 5601.5 27 7561.5 28 9445.0 29 11558.5 30 14142.0 31 17014.5 32 20550.0 33 23608.0 34 26430.0 35 29682.5 36 33770.0 37 37061.5 38 38351.5 39 39063.5 40 39633.0 41 40933.5 42 41818.0 43 42843.5 44 44234.5 45 44490.5 46 44323.5 47 45440.5 48 46405.5 49 44850.0 50 42012.0 51 39051.5 52 36576.5 53 34906.0 54 33493.0 55 30300.0 56 26000.5 57 22382.0 58 19615.0 59 16699.5 60 13180.0 61 10292.0 62 7878.5 63 5777.0 64 4055.5 65 2918.5 66 2160.5 67 1544.0 68 1108.5 69 807.0 70 586.0 71 426.0 72 309.5 73 256.5 74 192.5 75 130.0 76 100.5 77 74.0 78 48.0 79 27.0 80 18.5 81 17.0 82 14.0 83 8.5 84 6.0 85 6.0 86 5.5 87 3.5 88 1.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23823704586063135 2 0.21607958251793868 3 0.2072165971808616 4 0.21421835559715252 5 0.21200260926288322 6 0.23522363084602516 7 0.2374393771802944 8 0.2241448991746788 9 0.21980203635951104 10-11 0.2321215859780482 12-13 0.2326090501715874 14-15 0.21913731245923027 16-17 0.23371692333872202 18-19 0.2233029155676565 20-21 0.21847258855894947 22-23 0.21497170935080406 24-25 0.20921076888170395 26-27 0.22006792591962335 28-29 0.22516414248844266 30-31 0.22755714852945347 32-33 0.2253857171218696 34-35 0.23371692333872202 36-37 0.21599095266456791 38-39 0.21297753764996172 40-41 0.21191397940951245 42-43 0.21687725119827564 44-45 0.2314568620777674 46-47 0.2361542443064182 48-49 0.23699622791344055 50-51 0.2377052667404067 52-53 0.2525507671800108 54-55 0.23752800703366514 56-57 0.21164808984940015 58-59 0.19711279389659375 60-61 0.19569471624266144 62-63 0.20167723134518847 64-65 0.19529588190249297 66-67 0.19666964462973993 68-69 0.19959442979097536 70-71 0.20105682237159306 72-73 0.20229764031878386 74-75 0.2040259224595139 76-77 0.21802943929209564 78-79 0.21173671970277091 80-81 0.2051781105533339 82-83 0.2024749000255254 84-85 0.20260784480558158 86-87 0.20898919424827703 88-89 0.2069507076207493 90-91 0.21674430641821946 92-93 0.20584283445361468 94-95 0.20344982841260387 96-97 0.21346500184350098 98-99 0.21718745568507333 100-101 0.19569471624266144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1128288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.12085836061651 #Duplication Level Percentage of deduplicated Percentage of total 1 82.2742115979527 53.57767280201669 2 10.914292425940864 14.214961823520888 3 3.135150382616017 6.124910520167108 4 1.2522368230704173 3.261869471564681 5 0.6122392867156767 1.9934773936508232 6 0.3781646587946237 1.4775844309413333 7 0.24205012204874785 1.1033758199874513 8 0.18226928978236168 0.9495626082725871 9 0.130896452580491 0.7671680413561179 >10 0.7909668333161892 9.748238406290948 >50 0.06014290223897191 2.654564094762912 >100 0.02574464680984922 2.891407545548562 >500 9.535039109400906E-4 0.3648406867803637 >1k 6.810742221000646E-4 0.8703663551395402 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3159 0.27998170679826423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2245 0.19897402081737997 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1576 0.13968064891233445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1420 0.1258543917864942 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1412 0.12514535295952806 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.431492668538529E-5 0.0 12-13 0.0 0.0 0.0 8.862985337077058E-5 0.0 14-15 0.0 0.0 0.0 8.862985337077058E-5 0.0 16-17 0.0 0.0 0.0 2.2157463342692646E-4 0.0 18-19 0.0 0.0 0.0 2.6588956011231173E-4 0.0 20-21 0.0 0.0 0.0 3.545194134830823E-4 0.0 22-23 0.0 0.0 0.0 3.9883434016846765E-4 0.0 24-25 0.0 0.0 0.0 5.760940469100087E-4 0.0 26-27 0.0 0.0 0.0 7.5335375365155E-4 0.0 28-29 0.0 0.0 0.0 0.0016839672140446411 0.0 30-31 0.0 0.0 0.0 0.004431492668538529 0.0 32-33 0.0 0.0 0.0 0.008242576363481663 0.0 34-35 0.0 4.431492668538529E-5 0.0 0.010857157037919396 0.0 36-37 0.0 8.862985337077058E-5 0.0 0.014535295952806376 0.0 38-39 0.0 8.862985337077058E-5 0.0 0.020872330468816473 0.0 40-41 0.0 8.862985337077058E-5 0.0 0.03624961002864517 0.0 42-43 0.0 8.862985337077058E-5 0.0 0.06620650046796563 0.0 44-45 0.0 8.862985337077058E-5 0.0 0.09443510876655606 0.0 46-47 0.0 8.862985337077058E-5 0.0 0.15798271363339855 0.0 48-49 0.0 8.862985337077058E-5 0.0 0.22999446949714966 0.0 50-51 0.0 8.862985337077058E-5 0.0 0.28272923225275814 0.0 52-53 0.0 8.862985337077058E-5 0.0 0.35234798207549844 0.0 54-55 0.0 8.862985337077058E-5 0.0 0.42710726339374344 0.0 56-57 0.0 8.862985337077058E-5 0.0 0.5904077682293882 4.431492668538529E-5 58-59 0.0 8.862985337077058E-5 0.0 0.7537082730650331 8.862985337077058E-5 60-61 0.0 8.862985337077058E-5 0.0 0.9502892878414022 8.862985337077058E-5 62-63 0.0 8.862985337077058E-5 0.0 1.149617828072265 8.862985337077058E-5 64-65 0.0 8.862985337077058E-5 0.0 1.3779726452820555 8.862985337077058E-5 66-67 0.0 8.862985337077058E-5 0.0 1.6927415695283474 8.862985337077058E-5 68-69 0.0 8.862985337077058E-5 0.0 2.0992423920133865 8.862985337077058E-5 70-71 0.0 8.862985337077058E-5 0.0 2.4099786579313083 8.862985337077058E-5 72-73 0.0 8.862985337077058E-5 0.0 2.715973226693894 8.862985337077058E-5 74-75 0.0 8.862985337077058E-5 0.0 3.0941124961002866 8.862985337077058E-5 76-77 0.0 8.862985337077058E-5 0.0 3.5439533168836324 8.862985337077058E-5 78-79 0.0 8.862985337077058E-5 0.0 3.99397139737372 8.862985337077058E-5 80-81 0.0 8.862985337077058E-5 0.0 4.634720922317706 8.862985337077058E-5 82-83 0.0 8.862985337077058E-5 0.0 5.355902039195666 8.862985337077058E-5 84-85 0.0 8.862985337077058E-5 0.0 6.135091395104796 8.862985337077058E-5 86-87 0.0 8.862985337077058E-5 0.0 6.797555234124621 8.862985337077058E-5 88-89 0.0 8.862985337077058E-5 0.0 7.773990328710401 8.862985337077058E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 60 0.0039510275 31.666489 8 GTATCAA 5850 0.0 30.782902 1 CCGTCGT 70 0.00839173 27.142704 9 GGACCGA 160 6.239134E-8 26.719788 6 TCAACGC 6710 0.0 26.409163 4 ATCAACG 6790 0.0 26.237944 3 CAACGCA 6755 0.0 26.233232 5 TATCAAC 6875 0.0 25.9147 2 AACGCAG 6960 0.0 25.388916 6 GGTATCA 3930 0.0 23.938606 1 TACATGG 4185 0.0 23.27266 2 GTACATG 4290 0.0 23.148092 1 AGTACTT 3435 0.0 23.093029 12-13 ACATGGG 4095 0.0 22.854969 3 CGCAGAG 7595 0.0 22.82739 8 ACGCAGA 7740 0.0 22.645222 7 AGAGTAC 5275 0.0 22.601267 10-11 GAGTACT 3240 0.0 22.283825 12-13 GGCGACA 200 5.383881E-7 21.37583 6 GTACTTT 3405 0.0 20.92453 14-15 >>END_MODULE