FastQCFastQC Report
Thu 26 May 2016
SRR937212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128288
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.4042407612240846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.3235875946566834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22840.20243058509884002No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13860.12284097677188802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.031.5105151
GTATCAA55850.031.0586931
ATCGGAC1301.0015967E-525.5785338
TCAACGC67850.025.272014
ATCAACG67900.025.25343
TATCAAC68900.024.9558182
CAACGCA69250.024.6925095
AACGCAG70450.024.2708366
GTACATG42700.023.8176771
TACATGG41650.023.7208792
TAGGACG1452.3347571E-522.9304454
GAGTACT42150.021.75107212-13
TATTCCG1754.0500345E-621.713735
ATTCCGT1754.0515915E-621.7127676
CGCAGAG78950.021.2996318
AGTACTT43200.021.27738212-13
ACATGGG41750.021.2749023
ACGCAGA80500.021.0075327
GTACTTT43950.020.74940114-15
TACACCG1150.003723790220.6516445
Produced by FastQC (version 0.11.5)