##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937212_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1128288 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.239159682634224 33.0 31.0 34.0 31.0 34.0 2 32.44129335772427 34.0 31.0 34.0 31.0 34.0 3 32.50248074959585 34.0 31.0 34.0 31.0 34.0 4 36.13379208145438 37.0 35.0 37.0 35.0 37.0 5 36.06051823647863 37.0 35.0 37.0 35.0 37.0 6 36.09317567855016 37.0 35.0 37.0 35.0 37.0 7 36.03648270654301 37.0 35.0 37.0 35.0 37.0 8 36.004030885731304 37.0 35.0 37.0 35.0 37.0 9 37.7275110610057 39.0 37.0 39.0 35.0 39.0 10-11 37.6480570563544 39.0 37.0 39.0 35.0 39.0 12-13 37.556089845854956 39.0 37.0 39.0 35.0 39.0 14-15 38.78666040939902 40.0 38.0 41.0 35.0 41.0 16-17 38.749265701664825 40.0 38.0 41.0 35.0 41.0 18-19 38.74337314586347 40.0 38.0 41.0 34.5 41.0 20-21 38.64614575356647 40.0 38.0 41.0 34.0 41.0 22-23 38.499828944383 40.0 38.0 41.0 34.0 41.0 24-25 38.4370320343742 40.0 38.0 41.0 34.0 41.0 26-27 38.39398451459202 40.0 38.0 41.0 34.0 41.0 28-29 38.273615867579906 40.0 38.0 41.0 34.0 41.0 30-31 38.362001102555375 40.0 38.0 41.0 34.0 41.0 32-33 38.3121937838566 40.0 38.0 41.0 34.0 41.0 34-35 38.198227757452 40.0 38.0 41.0 34.0 41.0 36-37 38.0373317805383 40.0 38.0 41.0 33.5 41.0 38-39 37.91311482529284 40.0 37.0 41.0 33.0 41.0 40-41 37.75094036274426 40.0 37.0 41.0 33.0 41.0 42-43 37.54148985010919 40.0 37.0 41.0 33.0 41.0 44-45 37.26505112169943 40.0 37.0 41.0 32.0 41.0 46-47 36.9262107724269 40.0 37.0 41.0 31.0 41.0 48-49 36.61626951629371 40.0 36.0 41.0 30.5 41.0 50-51 36.39606731614623 39.5 36.0 41.0 30.0 41.0 52-53 36.162787337984625 39.0 36.0 41.0 30.0 41.0 54-55 35.95514088601492 39.0 35.0 40.5 29.0 41.0 56-57 35.70574091012224 39.0 35.0 40.0 28.0 41.0 58-59 35.4590410427125 38.5 35.0 40.0 28.0 41.0 60-61 35.14903420048782 38.0 34.5 40.0 27.5 41.0 62-63 34.80089746589523 38.0 34.0 40.0 26.5 41.0 64-65 34.42620323888936 37.5 34.0 40.0 26.0 41.0 66-67 34.03445884384129 37.0 34.0 39.5 26.0 41.0 68-69 33.603373429479 36.0 33.0 39.0 25.0 40.5 70-71 33.194031133894896 36.0 33.0 39.0 24.5 40.0 72-73 32.721930925437476 35.0 32.0 38.0 23.5 40.0 74-75 32.26077162922942 35.0 32.0 37.0 22.0 39.0 76-77 30.952689384270684 34.0 30.5 36.0 21.0 38.5 78-79 31.55639384625202 35.0 31.0 36.0 21.5 39.0 80-81 31.359657729232254 35.0 31.0 36.0 20.5 38.0 82-83 31.024636440341474 34.0 31.0 35.5 20.0 37.0 84-85 30.665338991463173 34.0 31.0 35.0 18.5 37.0 86-87 30.27596145664937 34.0 31.0 35.0 17.0 36.0 88-89 29.829901585410816 34.0 30.0 35.0 9.0 36.0 90-91 29.347142307637768 34.0 29.5 35.0 4.5 35.5 92-93 29.023118210953232 34.0 29.0 35.0 2.0 35.0 94-95 28.618470638702178 34.0 29.0 35.0 2.0 35.0 96-97 28.17006384894637 34.0 29.0 35.0 2.0 35.0 98-99 27.47286419779347 33.5 28.0 35.0 2.0 35.0 100-101 25.03474556141694 31.0 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.20529602108800304 1101 2 -0.06275019664131065 1101 3 -0.13797616050361938 1101 4 -0.08539819508474267 1101 5 -0.16099139541849183 1101 6 -0.14268651387469333 1101 7 -0.1843950937379617 1101 8 -0.22337581728547207 1101 9 -0.16020840582902451 1101 10-11 -0.2243038600393632 1101 12-13 -0.22408913917295337 1101 14-15 -0.250291394313642 1101 16-17 -0.23443685156051686 1101 18-19 -0.25465138839376067 1101 20-21 -0.14351117340311959 1101 22-23 -0.2776207820705423 1101 24-25 -0.21292381531882398 1101 26-27 -0.3283216480634721 1101 28-29 -0.4190489625984668 1101 30-31 -0.3732704312270201 1101 32-33 -0.3394327476770371 1101 34-35 -0.4005916126801097 1101 36-37 -0.3403543776929254 1101 38-39 -0.20724080337146233 1101 40-41 -0.2197061210939779 1101 42-43 -0.3261945132766684 1101 44-45 -0.22279990344130596 1101 46-47 -0.21634284260116488 1101 48-49 -0.20933308736306344 1101 50-51 -0.25589125054891326 1101 52-53 -0.3273445770628953 1101 54-55 -0.3896680146785769 1101 56-57 -0.3020941574211733 1101 58-59 -0.27631980923373334 1101 60-61 -0.26210466795816956 1101 62-63 -0.43745279862918096 1101 64-65 -0.33033843458477463 1101 66-67 -0.21402508551688726 1101 68-69 -0.12680541462985673 1101 70-71 -0.1988080019859808 1101 72-73 -0.13313147363113842 1101 74-75 -0.3007493724475623 1101 76-77 -0.27559194746597626 1101 78-79 -0.3538732922134855 1101 80-81 -0.29466811701278317 1101 82-83 -0.3684768998917889 1101 84-85 -0.5597548051651806 1101 86-87 -0.3957490029238393 1101 88-89 -0.30942738448784723 1101 90-91 -0.3015107424126988 1101 92-93 -0.3966077766044229 1101 94-95 -0.31536818779405706 1101 96-97 -0.4916681525186526 1101 98-99 -0.5546990682736173 1101 100-101 0.24466045208777842 1102 1 -0.12233203216833033 1102 2 -0.046545210491728994 1102 3 -0.073100814243233 1102 4 -0.1052873917606405 1102 5 -0.1348418109309577 1102 6 -0.13653692938716233 1102 7 -0.18558622947203673 1102 8 -0.13096584498629937 1102 9 -0.22588707618913162 1102 10-11 -0.14399915089808957 1102 12-13 -0.2032165629956637 1102 14-15 -0.1916071837873261 1102 16-17 -0.23821801499542516 1102 18-19 -0.27413892301979814 1102 20-21 -0.1269322260347039 1102 22-23 -0.19238532500682481 1102 24-25 -0.2615941754296287 1102 26-27 -0.17808619099976397 1102 28-29 -0.23202680193364955 1102 30-31 -0.12525104064530268 1102 32-33 -0.05580393881276535 1102 34-35 -0.13753067085185222 1102 36-37 -0.17873387907796712 1102 38-39 -0.16884745157091174 1102 40-41 -0.1428224645842846 1102 42-43 -0.24112526119910882 1102 44-45 -0.23466970953548838 1102 46-47 -0.10076112514963853 1102 48-49 0.13396331152890184 1102 50-51 0.1432638741048322 1102 52-53 0.1839989132418154 1102 54-55 0.12578904903332955 1102 56-57 0.09152080102758475 1102 58-59 0.11481592483828962 1102 60-61 -0.10754788125457537 1102 62-63 -0.02062454378152978 1102 64-65 -0.060269182507212804 1102 66-67 0.1051715903833852 1102 68-69 -0.05467245064093618 1102 70-71 -0.10988833439595425 1102 72-73 -0.059793523492629674 1102 74-75 -0.13453053588246888 1102 76-77 0.061360961121280866 1102 78-79 -0.014496560911545942 1102 80-81 -0.13542988986596782 1102 82-83 -0.16537440681699778 1102 84-85 -0.11794040073304757 1102 86-87 -0.16477947383797087 1102 88-89 0.10636208919636303 1102 90-91 0.021148537365693443 1102 92-93 0.043586129212751956 1102 94-95 0.25326062107020775 1102 96-97 0.2900492990048953 1102 98-99 0.3793314026405099 1102 100-101 0.8751036653841737 1103 1 -0.02626996758214517 1103 2 0.018024414304655068 1103 3 0.037295740178315384 1103 4 -0.03144120959776586 1103 5 -0.06038639454421002 1103 6 -0.016688961855109596 1103 7 -0.05394746303329612 1103 8 -0.14020743120399004 1103 9 -0.07765726052389965 1103 10-11 -0.05768207301365891 1103 12-13 -0.055971461257229294 1103 14-15 -0.19006845324003052 1103 16-17 -0.12982677986965996 1103 18-19 -0.15177763424093627 1103 20-21 -0.2355526142144555 1103 22-23 -0.12608554863812316 1103 24-25 0.016411732853939043 1103 26-27 -0.18720169702309164 1103 28-29 -0.2564532290786232 1103 30-31 -0.2739060318565052 1103 32-33 -0.38265533903916804 1103 34-35 -0.16255436840663862 1103 36-37 -0.26477210806536533 1103 38-39 -0.3260308016229061 1103 40-41 -0.30058651976507633 1103 42-43 -0.2592964541754199 1103 44-45 -0.305404379748353 1103 46-47 -0.11019229180556067 1103 48-49 -0.23797970390441492 1103 50-51 -0.2100781713392621 1103 52-53 -0.3081095709429462 1103 54-55 -0.4338855352241282 1103 56-57 -0.6709864462828605 1103 58-59 -0.6004499019072753 1103 60-61 -0.6855344633769533 1103 62-63 -0.5653029472565549 1103 64-65 -0.5464767454782873 1103 66-67 -0.5497883308395046 1103 68-69 -0.5117398437258558 1103 70-71 -0.2851867395275107 1103 72-73 -0.3117247591705592 1103 74-75 -0.07609558682070627 1103 76-77 0.0270064088900277 1103 78-79 -0.17870543069923173 1103 80-81 -0.32704877796749443 1103 82-83 -0.2743380644310456 1103 84-85 -0.09939372805379776 1103 86-87 -0.20086877910848955 1103 88-89 -0.15854436298058872 1103 90-91 -0.15490818951883867 1103 92-93 -0.3163157675119237 1103 94-95 -0.1923178293397818 1103 96-97 -0.24933557689353947 1103 98-99 0.054326110953518025 1103 100-101 0.3324397841388347 1104 1 0.028212750841824175 1104 2 0.06093401388500297 1104 3 0.03851506756654999 1104 4 -0.03031047578649293 1104 5 -0.0978335006816522 1104 6 0.036611731739341735 1104 7 -0.04807930426427731 1104 8 4.8383183733591295E-4 1104 9 0.01751089241658832 1104 10-11 0.1028891224167765 1104 12-13 -0.008738261979971185 1104 14-15 -0.07827385045399637 1104 16-17 0.0044227975622916915 1104 18-19 -0.06087945856919674 1104 20-21 0.07201514238634843 1104 22-23 -0.19884885224597326 1104 24-25 -0.08222206093285678 1104 26-27 -0.09612866560733124 1104 28-29 -0.03317176961601831 1104 30-31 -0.06914762420947795 1104 32-33 -0.03469590557177327 1104 34-35 0.10054873819708376 1104 36-37 0.09938246441233645 1104 38-39 0.06258375803204785 1104 40-41 -0.015583314076437205 1104 42-43 0.042752855144392754 1104 44-45 0.09605974568150089 1104 46-47 0.17707821003041602 1104 48-49 0.05715814070710934 1104 50-51 0.04261752138091168 1104 52-53 0.09046244048096241 1104 54-55 0.012437248479315599 1104 56-57 -0.07130468373696175 1104 58-59 -0.08158296712846891 1104 60-61 0.08342164783130102 1104 62-63 0.07193316628310242 1104 64-65 -0.0356893117777588 1104 66-67 0.08860649343047555 1104 68-69 0.03986125203680757 1104 70-71 0.07277094538244455 1104 72-73 0.020815894789919298 1104 74-75 0.1509172942191057 1104 76-77 0.259144894639288 1104 78-79 0.09656530427774257 1104 80-81 -0.04229969596014982 1104 82-83 -0.08461725076898219 1104 84-85 -0.0019477876213223055 1104 86-87 0.12179485672528045 1104 88-89 0.2653094576174162 1104 90-91 0.2911208365346667 1104 92-93 0.5421641532201384 1104 94-95 0.43296514553927423 1104 96-97 0.4107879878322258 1104 98-99 0.5517044404983125 1104 100-101 0.9750113292807612 1105 1 -0.18955065207888566 1105 2 -0.23029125599360611 1105 3 -0.1585356598105463 1105 4 -0.11114944471719923 1105 5 -0.21121695932826867 1105 6 -0.18847388733627923 1105 7 -0.23512009058762828 1105 8 -0.24553232587538076 1105 9 -0.22004668868700605 1105 10-11 -0.1290196999183948 1105 12-13 -0.21703050095729992 1105 14-15 -0.2875201505618037 1105 16-17 -0.33552287667917824 1105 18-19 -0.21183814018944958 1105 20-21 -0.301897572361419 1105 22-23 -0.29640979370577014 1105 24-25 -0.3113356269708518 1105 26-27 -0.17772119441539047 1105 28-29 -0.3872555825001811 1105 30-31 -0.3167557506708789 1105 32-33 -0.2768070493530175 1105 34-35 -0.21197250905512277 1105 36-37 -0.3211577128464498 1105 38-39 -0.4276121227313183 1105 40-41 -0.4115536869359602 1105 42-43 -0.414245506681965 1105 44-45 -0.38244181548398615 1105 46-47 -0.2506867330535485 1105 48-49 -0.15457986352133446 1105 50-51 -0.1354869576938711 1105 52-53 -0.341497347256535 1105 54-55 -0.0761988669489142 1105 56-57 0.05921827169631655 1105 58-59 -0.06260955594688511 1105 60-61 -0.1624883786315081 1105 62-63 0.048518764356501265 1105 64-65 0.1422479895761839 1105 66-67 0.10318035826014693 1105 68-69 0.24953263720362884 1105 70-71 0.353067496816891 1105 72-73 0.26795749179920136 1105 74-75 0.34304173941029603 1105 76-77 0.39373556124994735 1105 78-79 0.14894541656964577 1105 80-81 -0.08748976610758774 1105 82-83 -0.10721172578333693 1105 84-85 0.10855556573413239 1105 86-87 0.14795915014038385 1105 88-89 0.21938766990792047 1105 90-91 0.3759479882127472 1105 92-93 0.5710559357490901 1105 94-95 0.5442455812654607 1105 96-97 0.4035811439067736 1105 98-99 0.4873723813000339 1105 100-101 1.177047145077232 1106 1 -0.2790195089120395 1106 2 -0.1520864103098134 1106 3 -0.05849069310377786 1106 4 -0.028074942924490642 1106 5 -0.28944190602336306 1106 6 -0.17039861128239409 1106 7 -0.25119792170340105 1106 8 -0.17425562875443745 1106 9 -0.16127282059728998 1106 10-11 -0.2500393274392039 1106 12-13 -0.2679284950252665 1106 14-15 -0.36396578551553205 1106 16-17 -0.20762125847332413 1106 18-19 -0.261666313766888 1106 20-21 -0.1762301443379215 1106 22-23 -0.3003250240186901 1106 24-25 -0.3318044123337458 1106 26-27 -0.24805947210118262 1106 28-29 -0.16425124068173602 1106 30-31 -0.22732188448703283 1106 32-33 -0.24215662827405282 1106 34-35 -0.28070158911373255 1106 36-37 -0.24008140047376259 1106 38-39 -0.2670709290588178 1106 40-41 -0.25770985243725875 1106 42-43 -0.33815052270321644 1106 44-45 0.08372110991297177 1106 46-47 -0.11348541919188904 1106 48-49 -0.030756889405481047 1106 50-51 -0.023533630753149737 1106 52-53 0.15790483377764275 1106 54-55 0.05348988005826527 1106 56-57 0.0613612628459137 1106 58-59 0.17113207607656022 1106 60-61 -0.02305026890083184 1106 62-63 0.17525340718385962 1106 64-65 0.34318179322355036 1106 66-67 0.09027774244854925 1106 68-69 -0.029298541102761533 1106 70-71 0.010613639805065134 1106 72-73 -0.0316536653043471 1106 74-75 -0.004370739297520743 1106 76-77 0.06647992998885499 1106 78-79 3.1072192633985196E-4 1106 80-81 -0.10996269674569703 1106 82-83 0.10484203176571327 1106 84-85 0.09777956247032549 1106 86-87 0.25868147625919136 1106 88-89 0.3511676665726391 1106 90-91 0.49413635106726517 1106 92-93 0.7306300474332836 1106 94-95 0.3211351507762501 1106 96-97 0.43101939657797317 1106 98-99 0.5203343174294446 1106 100-101 0.958275927343081 1107 1 -0.17174338950905366 1107 2 -0.07226436668180014 1107 3 -0.11423122123236595 1107 4 -0.015043944072594684 1107 5 -0.05778289339419729 1107 6 -0.04591525881396308 1107 7 -0.04789711802946073 1107 8 -0.07223209258911112 1107 9 -0.12764767708408442 1107 10-11 -0.1332411070029238 1107 12-13 -0.027601284921942693 1107 14-15 -0.10400596921243022 1107 16-17 -0.1447050701840027 1107 18-19 0.008906303913938984 1107 20-21 -0.08285327866628478 1107 22-23 -0.13671997235393718 1107 24-25 -0.08532766147266813 1107 26-27 -0.22097853065456974 1107 28-29 -0.11057730267944521 1107 30-31 0.1172963703924168 1107 32-33 0.09143263693834314 1107 34-35 0.062460006753092046 1107 36-37 0.07180149275242087 1107 38-39 0.011387739138655206 1107 40-41 -0.0074945826324466225 1107 42-43 0.11093099279620589 1107 44-45 0.003131270647763529 1107 46-47 0.023763432702480713 1107 48-49 0.076992823568105 1107 50-51 0.022914417467283954 1107 52-53 0.23078801403021743 1107 54-55 0.27223988005826527 1107 56-57 0.228912927599076 1107 58-59 0.16011743772983067 1107 60-61 0.2905117288029615 1107 62-63 0.4608518572412663 1107 64-65 0.31185286096177833 1107 66-67 0.40765001569768344 1107 68-69 0.4123848687709497 1107 70-71 0.29253815186017107 1107 72-73 0.4254717652352582 1107 74-75 0.40667977735000704 1107 76-77 0.47905886225062133 1107 78-79 0.28438075636951154 1107 80-81 0.4994158910843822 1107 82-83 0.4755868652903956 1107 84-85 0.6259081503004076 1107 86-87 0.5093775152947835 1107 88-89 0.7372325345404924 1107 90-91 0.7953920915953994 1107 92-93 0.7751837213712882 1107 94-95 0.7270407190885315 1107 96-97 0.9465403495056428 1107 98-99 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0.5677015304230508 2316 80-81 0.7317359417234002 2316 82-83 0.6899903497369841 2316 84-85 0.6065040630083907 2316 86-87 0.5247063674770835 2316 88-89 0.44659472407509426 2316 90-91 0.5893455519281652 2316 92-93 0.8709487322225051 2316 94-95 0.8961026915987809 2316 96-97 0.9493646803472124 2316 98-99 1.0588028291030511 2316 100-101 0.6990557663923482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 7.0 5 21.0 6 30.0 7 102.0 8 226.0 9 479.0 10 820.0 11 1417.0 12 2154.0 13 3048.0 14 4090.0 15 5933.0 16 7819.0 17 10888.0 18 9904.0 19 6022.0 20 5059.0 21 5090.0 22 5192.0 23 5957.0 24 6664.0 25 7984.0 26 9786.0 27 12218.0 28 15117.0 29 18620.0 30 23817.0 31 30014.0 32 38435.0 33 50765.0 34 70629.0 35 101066.0 36 157769.0 37 254494.0 38 232873.0 39 23772.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08075016117576 19.773405069476283 15.676304104727437 22.469540664620524 2 15.492852888624181 20.70774483110695 40.25257735613602 23.546824924132846 3 19.112761989846565 24.44668382540628 30.628261578604043 25.81229260614311 4 12.64047831759267 17.474084630874387 38.31964888397288 31.565788167560054 5 13.144572034303906 37.909876022363484 34.87575646920767 14.069795474124941 6 31.434881874131428 37.09460705068209 17.683871493803 13.786639581383476 7 28.124911370146627 33.1663546895828 21.00545250857937 17.703281431691202 8 26.23523426642843 34.83188689412632 20.19218497404918 18.740693865396068 9 26.055049774525653 16.904460563260443 20.87782551972546 36.162664142488445 10-11 24.4843840637994 26.9211764919637 27.97609472416802 20.618344720068883 12-13 25.57399527836339 24.208602915856574 28.399000009306775 21.818401796473264 14-15 22.497314515442866 25.680012549987236 27.075578221163394 24.747094713406508 16-17 21.532622876428714 28.82296009529482 27.83930166765932 21.805115360617148 18-19 21.385807524320033 27.402046286054627 30.291202246235006 20.92094394339034 20-21 22.15413972319124 26.322490357071953 30.51583460960322 21.007535310133584 22-23 21.83609809102983 26.31411482182812 30.39320522371028 21.45658186343177 24-25 21.64700105736071 26.47478775234184 30.535965774483486 21.342245415813967 26-27 21.61792311523851 26.8281269110846 30.370313909771102 21.183636063905784 28-29 21.40582456463781 27.176456407056556 30.28067211696636 21.137046911339272 30-31 21.70558781443172 26.650834385445883 30.18965501196284 21.453922788159556 32-33 21.443328299157663 26.56981196290309 30.233016747497093 21.753842990442156 34-35 21.901367381377803 26.67820627357554 30.188258671544855 21.2321676735018 36-37 21.907581179442296 26.55094557016718 30.18565746762239 21.355815782768133 38-39 21.582233913520927 26.940368320224202 30.115542666765343 21.361855099489535 40-41 21.808315699677607 26.910694304421522 29.994261214451107 21.286728781449764 42-43 21.655594474555823 26.74553784526824 29.805965908965 21.79290177121094 44-45 21.862245553642126 26.840695109294387 29.81915884578603 21.477900491277456 46-47 22.01250921750475 27.173558524064777 29.25799973056525 21.555932527865227 48-49 21.949094557417965 27.26170091324201 29.082423991037746 21.70678053830228 50-51 21.96544676536487 27.435947204968947 29.070547590686065 21.528058438980118 52-53 22.11602888624181 27.499184605348987 28.613128917439518 21.77165759096968 54-55 21.932653719617687 27.386004282594516 28.858722241129925 21.822619756657875 56-57 22.092674919878615 27.413390907286082 28.689660795825176 21.804273377010126 58-59 22.08802185257665 27.228331773447913 28.86452749652571 21.81911887744973 60-61 21.950601264925268 27.309605348988907 29.111538897870048 21.628254488215777 62-63 21.644606696162683 27.323963385234972 29.39316025695567 21.63826966164667 64-65 21.672303525341047 27.316296902918403 29.056544073853484 21.954855497887063 66-67 21.764522887773335 27.268170892538073 28.743414801894552 22.22389141779404 68-69 21.92091026404606 27.05213562494682 28.923333404237216 22.103620706769902 70-71 21.901367381377803 26.96151159987521 28.979524731274285 22.157596287472703 72-73 21.643720397628975 26.85980884313225 29.207613658923965 22.288857100314814 74-75 21.563377435548368 27.237239073711677 28.70481650075158 22.49456698998837 76-77 21.492739442411867 27.010523908789246 29.018920701097596 22.477815947701295 78-79 21.64979154258487 26.9842894721915 28.802841118579654 22.563077866643976 80-81 21.64830402193768 27.132192429763 28.53515993714365 22.684343611155672 82-83 21.568952428981596 26.881456445044964 28.923943182618878 22.62564794335456 84-85 21.26879627610828 26.91618761976173 29.10194437206301 22.713071732066982 86-87 21.10888354746306 26.942544811253867 28.89984649309396 23.048725148189114 88-89 21.08428876315267 26.74206408292918 28.769516293712243 23.404130860205903 90-91 21.210094625480142 26.618784427547087 28.96352502627008 23.207595920702687 92-93 20.77212497230224 26.504764015067583 29.235807666740527 23.48730334588965 94-95 20.591506778411187 26.635885518590996 29.133031637312456 23.639576065685358 96-97 20.53296676025979 26.61483592841544 29.05002977963073 23.802167531694035 98-99 20.542593405035074 26.67863172868557 28.82485364956549 23.953921216713862 100-101 20.22981842420987 26.132350132052302 28.896828129909856 24.741003313827974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 51.0 1 39.0 2 47.5 3 65.5 4 53.0 5 59.5 6 118.0 7 262.0 8 451.5 9 563.5 10 615.5 11 629.5 12 639.5 13 741.0 14 874.0 15 959.5 16 1068.5 17 1191.0 18 1333.5 19 1491.5 20 1695.0 21 1957.5 22 2295.5 23 2734.0 24 3376.5 25 4356.5 26 5642.5 27 7425.0 28 9143.5 29 11212.5 30 13856.0 31 16554.5 32 20138.5 33 23080.5 34 25861.5 35 29136.5 36 32890.0 37 36232.5 38 37552.5 39 38159.0 40 39155.0 41 40347.0 42 40922.0 43 41972.0 44 43601.0 45 44710.0 46 44625.5 47 45922.0 48 47508.0 49 45888.0 50 42712.5 51 39576.0 52 37156.0 53 35461.5 54 34154.0 55 31110.5 56 26374.0 57 22525.5 58 20085.0 59 17102.0 60 13328.5 61 10437.0 62 8243.5 63 6133.5 64 4190.5 65 2848.0 66 2002.5 67 1480.0 68 1094.0 69 825.5 70 598.5 71 403.0 72 296.0 73 244.0 74 202.0 75 167.5 76 125.0 77 73.5 78 39.5 79 29.5 80 31.0 81 25.0 82 14.0 83 8.0 84 5.5 85 4.0 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056457216597180865 2 0.0 3 0.0 4 0.0 5 0.0017725970674154117 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.329447800561559E-4 12-13 0.00686881363623472 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.419836070223206E-4 24-25 6.20408973595394E-4 26-27 1.7725970674154116E-4 28-29 0.0028361553078646586 30-31 2.2157463342692646E-4 32-33 0.0 34-35 0.0 36-37 4.431492668538529E-5 38-39 3.10204486797697E-4 40-41 4.431492668538529E-5 42-43 0.014313721319379448 44-45 4.431492668538529E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.862985337077058E-5 82-83 5.760940469100088E-4 84-85 5.317791202246236E-4 86-87 0.0 88-89 0.0 90-91 0.009173189823874755 92-93 0.0033679344280892823 94-95 0.0 96-97 0.0 98-99 2.658895601123118E-4 100-101 0.012097974985110185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1128288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.86636883832796 #Duplication Level Percentage of deduplicated Percentage of total 1 80.50533918177155 49.80573007274173 2 11.647098520706317 14.411273859567217 3 3.5257854918311433 6.543826370473531 4 1.4821477821692526 3.6678040545837094 5 0.7561102464801054 2.338889769558863 6 0.44515910256304086 1.652422633854249 7 0.2973150492753348 1.2875661749757459 8 0.20400009741443348 1.0096596215756939 9 0.1539339100496403 0.8570998850271341 >10 0.8748332648917364 10.100117583151324 >50 0.07177734228132575 2.999821591273195 >100 0.03434450102544452 3.5975263975086227 >500 0.001580706996519556 0.6719240786001903 >1k 5.748025441889294E-4 1.0563379071086878 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4561 0.4042407612240846 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3651 0.3235875946566834 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2284 0.20243058509884002 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1386 0.12284097677188802 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.862985337077058E-5 0.0 18-19 0.0 0.0 0.0 2.2157463342692646E-4 0.0 20-21 0.0 0.0 0.0 2.6588956011231173E-4 0.0 22-23 0.0 0.0 0.0 3.10204486797697E-4 0.0 24-25 0.0 0.0 0.0 4.874641935392382E-4 0.0 26-27 0.0 0.0 0.0 6.647239002807794E-4 0.0 28-29 0.0 0.0 0.0 0.0015953373606738706 0.0 30-31 0.0 0.0 0.0 0.004431492668538529 0.0 32-33 0.0 0.0 0.0 0.00788805694999858 0.0 34-35 0.0 0.0 0.0 0.010724212257863242 0.0 36-37 0.0 0.0 0.0 0.014446666099435606 0.0 38-39 0.0 0.0 0.0 0.020960960322187243 0.0 40-41 0.0 0.0 0.0 0.036382554808701326 0.0 42-43 0.0 0.0 0.0 0.06638376017470717 0.0 44-45 0.0 0.0 0.0 0.09412490427975836 0.0 46-47 0.0 0.0 0.0 0.1578497688533424 0.0 48-49 0.0 0.0 0.0 0.2299058396437789 0.0 50-51 0.0 0.0 0.0 0.2828178621061289 0.0 52-53 0.0 0.0 0.0 0.3527911313423523 0.0 54-55 0.0 0.0 0.0 0.42772767236733883 0.0 56-57 0.0 0.0 0.0 0.5917372160299499 0.0 58-59 0.0 0.0 0.0 0.75565812983919 0.0 60-61 0.0 0.0 0.0 0.9535685924161207 0.0 62-63 0.0 0.0 0.0 1.15303007742704 0.0 64-65 0.0 0.0 0.0 1.3818723588303694 0.0 66-67 0.0 0.0 0.0 1.6973503219036274 0.0 68-69 0.0 0.0 0.0 2.1048703877024306 0.0 70-71 0.0 0.0 0.0 2.417157676054341 0.0 72-73 0.0 0.0 0.0 2.724969156811027 0.0 74-75 0.0 0.0 0.0 3.106830880058992 0.0 76-77 0.0 0.0 0.0 3.5597294307836296 0.0 78-79 0.0 0.0 0.0 4.014400578575683 0.0 80-81 0.0 0.0 0.0 4.663436994809835 0.0 82-83 0.0 0.0 0.0 5.390866516350435 0.0 84-85 0.0 0.0 0.0 6.176880370969114 0.0 86-87 0.0 0.0 0.0 6.846345968405229 0.0 88-89 0.0 0.0 0.0 7.834657463342693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3680 0.0 31.510515 1 GTATCAA 5585 0.0 31.058693 1 ATCGGAC 130 1.0015967E-5 25.578533 8 TCAACGC 6785 0.0 25.27201 4 ATCAACG 6790 0.0 25.2534 3 TATCAAC 6890 0.0 24.955818 2 CAACGCA 6925 0.0 24.692509 5 AACGCAG 7045 0.0 24.270836 6 GTACATG 4270 0.0 23.817677 1 TACATGG 4165 0.0 23.720879 2 TAGGACG 145 2.3347571E-5 22.930445 4 GAGTACT 4215 0.0 21.751072 12-13 TATTCCG 175 4.0500345E-6 21.71373 5 ATTCCGT 175 4.0515915E-6 21.712767 6 CGCAGAG 7895 0.0 21.299631 8 AGTACTT 4320 0.0 21.277382 12-13 ACATGGG 4175 0.0 21.274902 3 ACGCAGA 8050 0.0 21.007532 7 GTACTTT 4395 0.0 20.749401 14-15 TACACCG 115 0.0037237902 20.651644 5 >>END_MODULE