FastQCFastQC Report
Thu 26 May 2016
SRR937211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442101
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.5641244873908903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.44673049823456634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.32096738075688586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6330.14317995209239517No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.1115129800656411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11800.051.1750951
GTATCAA20200.038.6037451
TGGTATC5800.037.6895072
GTGGTAT6300.035.4727021
GTATGGT1201.4888428E-731.6990091
ACGCACT600.00394614531.6702588
TATCAAC27450.028.3917452
ATCAACG27500.028.3401223
TCAACGC27450.028.2186244
CAACGCA27750.027.9103935
AACGCAG28900.026.7936946
GTACATG23100.025.1122021
TACATGG22850.024.748642
TCTAGAC1801.9368599E-723.7607753
GTAATAC1000.00164767923.7607753
ACATGGG23200.023.7607753
TGGATCG1050.00218975722.6267435
CGCAGAG34200.022.502558
CATGGGG9950.022.4473654
ACGCAGA34350.022.4042877
Produced by FastQC (version 0.11.5)