##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937211_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 442101 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750014137041084 33.0 31.0 34.0 30.0 34.0 2 31.84939414296733 33.0 31.0 34.0 30.0 34.0 3 31.879988961798322 33.0 31.0 34.0 30.0 34.0 4 35.511555051899904 37.0 35.0 37.0 33.0 37.0 5 35.52123157378065 37.0 35.0 37.0 33.0 37.0 6 35.56987882859347 37.0 35.0 37.0 33.0 37.0 7 35.50897193175315 37.0 35.0 37.0 33.0 37.0 8 35.48901495359657 37.0 35.0 37.0 33.0 37.0 9 37.1038382632023 39.0 37.0 39.0 34.0 39.0 10-11 36.98669874078547 39.0 37.0 39.0 33.0 39.0 12-13 36.83390786268296 39.0 37.0 39.0 33.0 39.0 14-15 37.94668073584995 40.0 38.0 41.0 33.0 41.0 16-17 37.86033621276586 40.0 38.0 41.0 33.0 41.0 18-19 37.801599634472666 40.0 38.0 41.0 33.0 41.0 20-21 37.68983671152067 40.0 37.5 41.0 32.5 41.0 22-23 37.75129664940817 40.0 38.0 41.0 33.0 41.0 24-25 37.712964910733064 40.0 37.5 41.0 32.5 41.0 26-27 37.62843671468737 40.0 37.0 41.0 32.5 41.0 28-29 37.47369379395206 40.0 37.0 41.0 32.0 41.0 30-31 37.19297965849432 39.5 37.0 41.0 31.5 41.0 32-33 36.92429105566375 39.0 36.0 41.0 31.0 41.0 34-35 36.9107036627377 39.0 36.0 41.0 31.0 41.0 36-37 36.776802133449145 39.0 36.0 41.0 30.5 41.0 38-39 36.61136143098523 39.0 35.5 41.0 30.0 41.0 40-41 36.358721197192494 39.0 35.0 41.0 30.0 41.0 42-43 36.07075193225077 39.0 35.0 40.5 30.0 41.0 44-45 35.782449033139486 39.0 35.0 40.0 28.5 41.0 46-47 35.3252254575312 38.5 35.0 40.0 26.5 41.0 48-49 34.92970271499047 38.0 34.5 40.0 24.5 41.0 50-51 33.947566280103416 37.5 33.5 39.5 23.5 40.5 52-53 34.03376377796024 38.0 33.5 39.5 23.5 40.5 54-55 34.29997557119301 38.0 34.0 40.0 23.0 41.0 56-57 34.032123881194565 38.0 34.0 40.0 22.0 41.0 58-59 33.74102863372849 38.0 33.0 40.0 21.5 41.0 60-61 33.388079873151156 37.0 33.0 40.0 19.5 41.0 62-63 32.98929543249167 37.0 32.5 39.5 18.0 41.0 64-65 32.53608790751434 36.0 32.0 39.0 14.0 41.0 66-67 32.08788715700711 35.5 31.0 39.0 10.5 40.0 68-69 31.57388470055485 35.0 31.0 38.5 7.5 40.0 70-71 31.099065598132555 35.0 30.5 38.0 4.5 40.0 72-73 30.584209264398858 35.0 30.0 37.0 2.0 39.5 74-75 30.03462331005811 34.0 29.0 36.5 2.0 39.0 76-77 29.486702133675337 34.0 29.0 36.0 2.0 39.0 78-79 28.924891597168973 34.0 28.5 35.5 2.0 38.0 80-81 28.344657668722757 34.0 27.0 35.0 2.0 37.0 82-83 27.79057839724407 33.5 26.0 35.0 2.0 36.5 84-85 27.22732927543706 33.0 25.5 35.0 2.0 36.0 86-87 26.61375794218968 32.5 25.0 35.0 2.0 36.0 88-89 26.02807277070172 32.0 23.5 35.0 2.0 35.0 90-91 25.4378614841405 32.0 20.5 34.5 2.0 35.0 92-93 24.742891330261635 31.5 18.0 34.0 2.0 35.0 94-95 23.923774205441745 31.0 5.0 34.0 2.0 35.0 96-97 23.055629822144713 31.0 2.0 34.0 2.0 35.0 98-99 21.846092861133542 30.0 2.0 34.0 2.0 35.0 100-101 18.790622504812248 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08093395609534682 1101 2 0.09748246062323673 1101 3 0.04071969181133639 1101 4 0.03978687666107561 1101 5 -0.02736689310570739 1101 6 0.030496562736935573 1101 7 0.060327757714908614 1101 8 0.11899499616652065 1101 9 0.24047263920913764 1101 10-11 0.25063552494261643 1101 12-13 0.30378079139475034 1101 14-15 0.40702712407397 1101 16-17 0.4610704059142705 1101 18-19 0.5406902511458611 1101 20-21 0.5619353909608975 1101 22-23 0.42878784109850443 1101 24-25 0.4124256359968399 1101 26-27 0.37601645507784554 1101 28-29 0.42200730369685147 1101 30-31 0.34307030543630646 1101 32-33 0.3505656287501111 1101 34-35 0.512250337649327 1101 36-37 0.3300469830182564 1101 38-39 0.4438244112781007 1101 40-41 0.5615772861691823 1101 42-43 0.5787296017760966 1101 44-45 0.4175798496230456 1101 46-47 0.6921673587569401 1101 48-49 0.5137395937050258 1101 50-51 0.5677063253282384 1101 52-53 0.6745342436996182 1101 54-55 0.5829054392924533 1101 56-57 0.6610333286421053 1101 58-59 0.608767582633071 1101 60-61 0.8608422678390468 1101 62-63 1.010942377289723 1101 64-65 0.5138270964194263 1101 66-67 0.5783658322264564 1101 68-69 0.618837554359029 1101 70-71 0.553234851808817 1101 72-73 0.4799094309345264 1101 74-75 0.6578397170168557 1101 76-77 0.6507020440440776 1101 78-79 0.8951894251015808 1101 80-81 1.069198542238972 1101 82-83 0.7031113859362677 1101 84-85 1.3058473358741338 1101 86-87 1.3037534383826745 1101 88-89 1.300231958555628 1101 90-91 1.1320418195320272 1101 92-93 1.1673046076881626 1101 94-95 1.5425545020639326 1101 96-97 1.0098581049479805 1101 98-99 0.9071408001290528 1101 100-101 1.116845871919967 1102 1 0.2966227455596986 1102 2 0.049670659702009345 1102 3 -0.02471691058566705 1102 4 0.0890850157204568 1102 5 0.18865387035904746 1102 6 0.1380863183210792 1102 7 -0.01670530010515847 1102 8 0.0949231087057072 1102 9 -0.17193338008307535 1102 10-11 0.21071187896270516 1102 12-13 0.2107111258962746 1102 14-15 0.21235832472584804 1102 16-17 0.42061107461552893 1102 18-19 0.4951287058068914 1102 20-21 0.27915390152936226 1102 22-23 0.30884148507902154 1102 24-25 0.3032722074221752 1102 26-27 0.3697372423089149 1102 28-29 0.4462168849191883 1102 30-31 0.39742727556996016 1102 32-33 0.5471319613050625 1102 34-35 0.934609918305803 1102 36-37 1.023206809637287 1102 38-39 0.7679668432140048 1102 40-41 0.8462148120574966 1102 42-43 0.9954588279371492 1102 44-45 0.891561387341703 1102 46-47 0.801996052627878 1102 48-49 0.7220174408536266 1102 50-51 0.8171508347423426 1102 52-53 0.9147777918980964 1102 54-55 1.1242229988308594 1102 56-57 1.2332829429109466 1102 58-59 1.1147921562539622 1102 60-61 1.1415262851771502 1102 62-63 0.9281488160763658 1102 64-65 1.1992911621200975 1102 66-67 0.9977112398565744 1102 68-69 1.2001692982410894 1102 70-71 1.147125021228728 1102 72-73 1.3982985270975838 1102 74-75 1.5022740597271955 1102 76-77 1.4666620713564846 1102 78-79 1.5167843338275908 1102 80-81 1.5205511476690106 1102 82-83 1.6132199942484426 1102 84-85 1.9771195130577155 1102 86-87 1.8404158170518343 1102 88-89 1.7316057650525103 1102 90-91 1.4401287816304063 1102 92-93 2.039945423137027 1102 94-95 2.1552106335859946 1102 96-97 2.0267831203206406 1102 98-99 2.4539453617536147 1102 100-101 2.182146666771356 1103 1 0.1389196137014963 1103 2 0.31923509129427075 1103 3 0.28986744475415804 1103 4 0.20571424467573252 1103 5 0.25959187867280065 1103 6 0.37299719935521836 1103 7 0.35013693651838906 1103 8 0.262570901602011 1103 9 0.36487606626911173 1103 10-11 0.31193570483862487 1103 12-13 0.5070714918391488 1103 14-15 0.3717009701519842 1103 16-17 0.5455024191887858 1103 18-19 0.48665573411324203 1103 20-21 0.4744948428941669 1103 22-23 0.25083454834445007 1103 24-25 0.5109761999174225 1103 26-27 0.31741266165431625 1103 28-29 -0.001781273317533305 1103 30-31 0.2303539579002276 1103 32-33 0.24601442410403251 1103 34-35 0.2990961610226677 1103 36-37 0.4608515042402388 1103 38-39 0.5486076735566172 1103 40-41 0.6047071480846284 1103 42-43 0.19164104201201582 1103 44-45 0.22316484021752814 1103 46-47 -0.14943174422735694 1103 48-49 -0.3094797233473088 1103 50-51 -0.2419448078523132 1103 52-53 -0.45797271802935313 1103 54-55 0.2004539249623818 1103 56-57 0.10784079062983665 1103 58-59 -0.0310255780329598 1103 60-61 0.13618172913676574 1103 62-63 0.14604920594494075 1103 64-65 -0.00901312471301452 1103 66-67 0.18974733497418228 1103 68-69 0.020590924328718785 1103 70-71 0.10682784325759442 1103 72-73 0.006084710430489082 1103 74-75 -0.05173091475491631 1103 76-77 0.28574110365619276 1103 78-79 0.18956582668860733 1103 80-81 0.4672841694517338 1103 82-83 0.5862734484152199 1103 84-85 0.854254968721996 1103 86-87 1.0805550681254772 1103 88-89 1.470936017305231 1103 90-91 1.1166110137786447 1103 92-93 1.1041274306589948 1103 94-95 1.1268160659270166 1103 96-97 1.0369020603600596 1103 98-99 0.8801995250853132 1103 100-101 0.5707608690038448 1104 1 0.2068428409167602 1104 2 0.3222519859040922 1104 3 0.2683565073140173 1104 4 0.21675703669197333 1104 5 0.34969646435260415 1104 6 0.21880994118566122 1104 7 0.2824818777128897 1104 8 0.4387671242584119 1104 9 0.3303493835123419 1104 10-11 0.3786665403747733 1104 12-13 0.38415937445800097 1104 14-15 0.45709345377456145 1104 16-17 0.5667739205487834 1104 18-19 0.3630253084916717 1104 20-21 0.4402602721551645 1104 22-23 0.5294998358592977 1104 24-25 0.30869102196852793 1104 26-27 0.4347938055841354 1104 28-29 0.42364875388240364 1104 30-31 0.381936151517813 1104 32-33 0.7371313455306918 1104 34-35 0.95767946562367 1104 36-37 0.9716357052899269 1104 38-39 1.2082301307454415 1104 40-41 0.9540874875092129 1104 42-43 0.9391748503865287 1104 44-45 1.0801400921042799 1104 46-47 1.1331507175586424 1104 48-49 0.9124104808437821 1104 50-51 0.8829067417779939 1104 52-53 1.2843413837980293 1104 54-55 1.2499597288929678 1104 56-57 1.3625097941926398 1104 58-59 1.5072075752444434 1104 60-61 1.3059754310101113 1104 62-63 0.9699774900502192 1104 64-65 1.385290212068199 1104 66-67 1.514695616780866 1104 68-69 1.5741048882409139 1104 70-71 1.118823590872811 1104 72-73 1.4866137683931733 1104 74-75 1.6274917799545072 1104 76-77 1.5556238757513547 1104 78-79 1.9498232683619783 1104 80-81 2.0904261454698165 1104 82-83 2.1138181750749894 1104 84-85 1.9758549763839497 1104 86-87 1.8987560411937103 1104 88-89 1.4433529808726355 1104 90-91 2.0319847256125314 1104 92-93 2.0815629744662196 1104 94-95 2.04356414086093 1104 96-97 1.9963990530428894 1104 98-99 2.214543278676853 1104 100-101 2.143161550917803 1105 1 0.11950064404556215 1105 2 0.23737382459888678 1105 3 0.2593182151527955 1105 4 0.3667696190012464 1105 5 0.34942384765168555 1105 6 0.24320913591786564 1105 7 0.2332640779393742 1105 8 0.16911775127682205 1105 9 0.53685653445811 1105 10-11 0.39048866846267316 1105 12-13 0.33355227803557597 1105 14-15 0.46622611325533114 1105 16-17 0.47668458615294185 1105 18-19 0.41432443192412194 1105 20-21 0.09796379100766472 1105 22-23 0.48620620670189396 1105 24-25 0.4310434943922985 1105 26-27 0.4577565200076492 1105 28-29 0.5555113550651427 1105 30-31 0.8774170054372021 1105 32-33 0.8276243623490345 1105 34-35 1.1609886129625124 1105 36-37 0.8044482708227818 1105 38-39 0.8023929877684779 1105 40-41 1.0127158060932828 1105 42-43 1.0341555575123067 1105 44-45 1.2087365355048618 1105 46-47 1.0944286607704896 1105 48-49 1.102078307878962 1105 50-51 1.3178457175780238 1105 52-53 0.984216609231062 1105 54-55 1.0748768953569154 1105 56-57 1.3802141518657578 1105 58-59 1.257186604728986 1105 60-61 1.3707481106225785 1105 62-63 1.0457229918746478 1105 64-65 1.2312722613069695 1105 66-67 1.341421490202844 1105 68-69 1.3458355848814385 1105 70-71 1.497084506026102 1105 72-73 1.993754228905697 1105 74-75 1.8239435687394732 1105 76-77 1.4645279566136615 1105 78-79 1.714555055469308 1105 80-81 2.1643000287898673 1105 82-83 2.237460334199259 1105 84-85 1.9794986034443056 1105 86-87 1.9272549716974225 1105 88-89 2.230722439413924 1105 90-91 2.128449096706774 1105 92-93 2.103424736828341 1105 94-95 2.5260590030846437 1105 96-97 2.6015242470201585 1105 98-99 3.254591720567557 1105 100-101 2.537349572559375 1106 1 -0.1075260459020555 1106 2 -0.9118653871746254 1106 3 -0.2694171303198942 1106 4 -0.10131469130057269 1106 5 -0.05524065721236582 1106 6 -0.1287913629596602 1106 7 -0.19448598442031795 1106 8 0.0037647954274859785 1106 9 -0.453465241545878 1106 10-11 -0.5635085877109347 1106 12-13 -0.29256396042787713 1106 14-15 -0.18935507906195426 1106 16-17 -0.123864179125178 1106 18-19 -0.014722712002040339 1106 20-21 -0.03994466496639859 1106 22-23 -0.0435206578528593 1106 24-25 -0.21152323047674315 1106 26-27 -0.19742557028918384 1106 28-29 -0.07858692380739996 1106 30-31 0.06173354322717728 1106 32-33 0.525161154566554 1106 34-35 0.10613079305729656 1106 36-37 -0.22396280425828508 1106 38-39 0.13498839809523844 1106 40-41 0.2758737008301253 1106 42-43 0.32021816664176583 1106 44-45 0.6720939812519262 1106 46-47 0.6791567725190575 1106 48-49 0.5610830472154547 1106 50-51 0.28844935938395366 1106 52-53 0.5552193530574598 1106 54-55 0.6285810609842528 1106 56-57 0.39417025064349787 1106 58-59 0.6197832622495625 1106 60-61 0.37203272025091394 1106 62-63 0.5176486591233456 1106 64-65 0.5303181580393215 1106 66-67 0.7731376288907903 1106 68-69 0.7214965976028189 1106 70-71 0.7633042615761205 1106 72-73 0.9848555010433842 1106 74-75 0.6987190738529669 1106 76-77 0.9423029628496273 1106 78-79 1.1193505156838235 1106 80-81 1.1749510639607337 1106 82-83 1.0807254876041021 1106 84-85 1.1605662013490203 1106 86-87 1.2386605591257052 1106 88-89 0.9282155798479472 1106 90-91 1.0140876599394986 1106 92-93 1.4714092716250455 1106 94-95 2.120066236808764 1106 96-97 1.772901148990723 1106 98-99 1.154703968010807 1106 100-101 1.2495658359667097 1107 1 0.24234743587932073 1107 2 0.2333522241614041 1107 3 0.08523874012545107 1107 4 -0.1833238006123139 1107 5 -0.19342385559293263 1107 6 -0.004854115996096198 1107 7 0.08987636375781705 1107 8 0.10907856060966736 1107 9 -0.18749864235559244 1107 10-11 0.19113463091255056 1107 12-13 0.26077612054377397 1107 14-15 0.10955686425788258 1107 16-17 0.17201132694769683 1107 18-19 0.27763558759309603 1107 20-21 0.31228205568137213 1107 22-23 0.2936250911349987 1107 24-25 0.16965085859207818 1107 26-27 0.17668880218045757 1107 28-29 0.11132957416831601 1107 30-31 0.018742652538911386 1107 32-33 0.15714496023456803 1107 34-35 0.5272846392111461 1107 36-37 0.5469633091020398 1107 38-39 0.6471341470830936 1107 40-41 0.6930042676317427 1107 42-43 0.6240263650223312 1107 44-45 0.4676911472438334 1107 46-47 0.6199082907376763 1107 48-49 0.7206984318308471 1107 50-51 0.4839959180884108 1107 52-53 0.537266417834779 1107 54-55 0.407262741146198 1107 56-57 0.464109521898564 1107 58-59 0.9071820476746666 1107 60-61 0.6720833275383598 1107 62-63 0.7201157643864988 1107 64-65 0.7273702835453975 1107 66-67 1.1453542280810787 1107 68-69 1.0825289862667837 1107 70-71 0.9083700510498041 1107 72-73 0.940738598209375 1107 74-75 1.30245136130236 1107 76-77 1.4461744203395064 1107 78-79 1.1913140784368572 1107 80-81 1.5971803149728778 1107 82-83 1.53379988031665 1107 84-85 1.2867807762478023 1107 86-87 1.0726307008261102 1107 88-89 1.0686508025200148 1107 90-91 1.4643153927330204 1107 92-93 1.5000023890339556 1107 94-95 1.7672069250678746 1107 96-97 1.591436068019064 1107 98-99 1.9209868627476503 1107 100-101 1.3095481234161 1108 1 -0.18187692309503944 1108 2 -0.17712854506936182 1108 3 -0.10983818295147074 1108 4 -0.09608662112513144 1108 5 -0.09180206072113606 1108 6 0.3702612686192879 1108 7 -0.1291877388062801 1108 8 -0.07483169580058302 1108 9 -0.21318453979149155 1108 10-11 0.11636860527152493 1108 12-13 0.018032530800191182 1108 14-15 -0.17870595625493735 1108 16-17 -0.06396944228308143 1108 18-19 -0.08191569445818914 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-0.04223593372542922 2316 44-45 -0.006302591575483518 2316 46-47 -0.4919264588150938 2316 48-49 -0.7389169527845425 2316 50-51 -0.5317464790493318 2316 52-53 -0.21638407411512617 2316 54-55 -0.21046422665344267 2316 56-57 0.3805227598234282 2316 58-59 -0.05451294338079293 2316 60-61 0.05685524167074618 2316 62-63 0.2289093690376589 2316 64-65 0.3080109364970909 2316 66-67 0.2672012763816163 2316 68-69 0.13664347642778196 2316 70-71 0.2867130743056201 2316 72-73 0.28035733702869337 2316 74-75 -0.16887578538815617 2316 76-77 0.30564445611768676 2316 78-79 0.4770922537499196 2316 80-81 0.6756854580855816 2316 82-83 0.756368082463343 2316 84-85 0.6009578782156026 2316 86-87 0.9281036435810321 2316 88-89 0.9072241477794059 2316 90-91 1.3684634249333776 2316 92-93 1.2484594552235784 2316 94-95 0.9002681951930995 2316 96-97 1.5571728748169171 2316 98-99 2.074838606933696 2316 100-101 2.5145291162604657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1962.0 3 405.0 4 377.0 5 446.0 6 445.0 7 520.0 8 736.0 9 817.0 10 1000.0 11 1322.0 12 1809.0 13 2264.0 14 2617.0 15 3287.0 16 4239.0 17 5667.0 18 4912.0 19 3446.0 20 3510.0 21 3733.0 22 4204.0 23 4478.0 24 5094.0 25 5779.0 26 6553.0 27 7816.0 28 8924.0 29 10661.0 30 13153.0 31 16145.0 32 20246.0 33 25933.0 34 34387.0 35 46784.0 36 62772.0 37 74905.0 38 46699.0 39 4054.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99030119375158 19.085962361554984 14.62464757904096 22.29908886565248 2 15.232690485151611 20.359453858676183 40.633431430786885 23.774424225385324 3 19.225284255155138 24.950290773468765 29.772482513915183 26.051942457460918 4 12.27129065754928 16.48135636259755 36.99003768177717 34.25731529807601 5 13.106863254122464 38.20101165603695 33.83719638415722 14.854928705683367 6 31.664279173504266 36.48784645094608 17.22712313118217 14.62075124436748 7 28.451462725267852 33.33952860060245 20.122076986017586 18.086931688112113 8 26.75846593227254 35.176571920757965 19.260619248379346 18.804342898590146 9 26.67277207410816 16.62854565114397 19.890556037892818 36.808126236855045 10-11 25.068407128396835 27.279311916102365 26.702810416473028 20.949470539027768 12-13 26.060794276692278 24.535326940099992 27.695863137505146 21.708015645702588 14-15 22.675098599211207 25.774853801169588 26.157577406047416 25.392470193571786 16-17 21.592511162991002 29.515741517260135 26.794691629456697 22.097055690292162 18-19 21.70682125673933 27.31165567146286 29.946991861811707 21.034531209986106 20-21 22.32671618010684 26.332250746214537 30.25177516335129 21.089257910327337 22-23 21.946393827346558 26.45927977399875 30.255769864913713 21.338556533740977 24-25 21.643528879833468 26.678731329330162 30.341871140790534 21.335868650045835 26-27 21.24725500105381 27.020761143732564 30.137085643967122 21.59489821124651 28-29 21.284514295298667 27.29978195533031 30.103197232963275 21.312506516407748 30-31 21.376028870718752 26.891864015650395 30.12576534833692 21.606341765293934 32-33 21.164153088205126 27.136787251187467 29.986932489819694 21.712127170787713 34-35 21.67231820263023 27.320605288568334 29.82050328892193 21.186573219879502 36-37 21.643076504651926 26.983443445939052 29.865711727842438 21.507768321566584 38-39 21.337808407307275 27.310377805325214 29.922562485977622 21.429251301389886 40-41 21.702024476827916 27.203362079612575 29.88627260356082 21.208340839998684 42-43 21.356393557430906 27.360974023251362 29.524173232718713 21.758459186599023 44-45 21.44254990770319 27.773238796617257 29.35299310238544 21.431218193294118 46-47 21.797158671084897 27.995000022665355 28.671107565242227 21.53673374100752 48-49 21.769267210212682 28.22007946923797 28.428725885528937 21.58192743502041 50-51 21.877064983434796 28.361920543687823 28.238149049441724 21.522865423435654 52-53 22.0499665023335 28.224873065949023 28.221358928346735 21.503801503370738 54-55 21.619572411667104 28.466108456565692 28.269926468588213 21.644392663178987 56-57 21.6691878417042 28.73063086031557 28.033766748817314 21.566414549162914 58-59 21.684137062163792 28.272130300599567 28.144784486470076 21.898948150766568 60-61 21.476700933276984 28.304794190727506 28.556213594323065 21.662291281672445 62-63 21.54019602288822 28.429437425641606 28.493569769418166 21.536796782052008 64-65 21.411875873117665 28.32974361356563 28.48938322911627 21.768997284200438 66-67 21.229988998127077 28.528389024413737 28.320588458570757 21.921033518888436 68-69 21.63015939920504 28.276362960646477 28.470569839694342 21.62290780045413 70-71 21.402195207345912 28.419516428929963 28.252842019627018 21.925446344097107 72-73 21.295002668461503 28.4913504454801 28.087398629623213 22.126248256435186 74-75 21.392813842022367 28.584070796460175 28.0619355715953 21.961179789922156 76-77 21.56071060686243 28.549493063118092 27.979493618385938 21.91030271163354 78-79 21.58318545362243 28.495343772167793 27.83742070587915 22.084050068330622 80-81 21.41156580141669 28.702714279400915 27.88139348042621 22.004326438756188 82-83 21.405650885160306 28.77156246884036 27.889574778596614 21.93321186740271 84-85 21.327552237757562 28.700035234924044 27.754017177875184 22.21839534944321 86-87 21.57810896121347 28.619030030839788 27.684319292656234 22.118541715290508 88-89 21.491855754794482 28.457550777598367 27.62459986969208 22.425993597915074 90-91 21.327483564521575 28.316046148511916 28.070392117315663 22.28607816965085 92-93 21.383893666927285 28.105630531098797 28.071523268858144 22.43895253311577 94-95 21.21562686602678 28.014621151948262 28.131327494994657 22.6384244870303 96-97 21.263491883622766 27.87478398821496 28.12328961160373 22.73843451655854 98-99 21.160969960417873 28.404283889859023 27.706695645966033 22.72805050375707 100-101 21.39783411172068 27.718747238254508 28.155981119291546 22.727437530733273 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 774.0 1 455.0 2 159.0 3 206.0 4 200.0 5 198.5 6 318.5 7 603.5 8 881.0 9 991.5 10 922.5 11 808.5 12 774.5 13 795.0 14 905.5 15 935.5 16 932.5 17 1035.0 18 1097.0 19 1123.0 20 1198.5 21 1381.5 22 1599.5 23 1889.0 24 2211.5 25 2566.5 26 3098.5 27 3864.0 28 4494.0 29 5023.0 30 5791.5 31 6704.0 32 7966.5 33 8689.0 34 9062.5 35 9697.0 36 10489.5 37 11435.5 38 12135.0 39 12572.5 40 12985.0 41 13700.5 42 14122.0 43 14467.0 44 14902.5 45 15649.5 46 15939.5 47 15764.0 48 16199.0 49 16133.5 50 15769.5 51 15339.5 52 14662.5 53 14398.0 54 14169.0 55 13056.0 56 11722.0 57 10620.0 58 9502.5 59 8390.5 60 6907.0 61 5588.5 62 4572.0 63 3306.0 64 2297.5 65 1709.0 66 1249.5 67 930.5 68 687.0 69 486.0 70 352.5 71 245.0 72 174.5 73 140.0 74 103.0 75 76.5 76 56.0 77 38.5 78 24.0 79 16.5 80 11.5 81 8.5 82 5.5 83 3.5 84 5.0 85 4.0 86 4.0 87 3.0 88 1.0 89 1.0 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2764074272620962 2 0.2359189415993178 3 0.23433559299798012 4 0.23501417096998198 5 0.23569274894198383 6 0.20651389614590332 7 0.2044781622298977 8 0.20832343740457498 9 0.21759733635526723 10-11 0.22438311607528597 12-13 0.2165794693972644 14-15 0.20832343740457498 16-17 0.2060615108312354 18-19 0.19181137341919607 20-21 0.19769238250987894 22-23 0.19554355226520637 24-25 0.19622213023720825 26-27 0.19090660278986024 28-29 0.20515674020189956 30-31 0.21759733635526723 32-33 0.21047226764924756 34-35 0.2087758227192429 36-37 0.20040669439788644 38-39 0.1904542174751923 40-41 0.18694823128651597 42-43 0.19893644212521572 44-45 0.19486497429320448 46-47 0.2033471989432279 48-49 0.20900201537657684 50-51 0.21838901065593608 52-53 0.23196057009597354 54-55 0.21013297866324665 56-57 0.18966254317452347 58-59 0.17710885069248883 60-61 0.18242437813983683 62-63 0.18683513495784898 64-65 0.17971006625182934 66-67 0.18378153408384057 68-69 0.1852517863565113 70-71 0.1858172679998462 72-73 0.1889839652025216 74-75 0.18909706153118858 76-77 0.19735309352387803 78-79 0.19543045593653938 80-81 0.19463878163587053 82-83 0.18660894230051506 84-85 0.17586479107715206 86-87 0.1782398139791586 88-89 0.18966254317452347 90-91 0.1877399055871848 92-93 0.19135898810452814 94-95 0.18604346065718014 96-97 0.19531735960787242 98-99 0.19599593757987427 100-101 0.18185889649650192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 442101.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.87329285221715 #Duplication Level Percentage of deduplicated Percentage of total 1 87.05881056255515 64.31321008053332 2 8.236070736894018 12.168513309962952 3 2.093782446366947 4.640238114878914 4 0.8501204951145385 2.5120480118107253 5 0.4817420104362131 1.779393430808511 6 0.2988434716583224 1.3245930779273103 7 0.19010083805173877 0.9830362416593602 8 0.14348342220235158 0.8479674294234105 9 0.11046426055195385 0.7344322802512294 >10 0.4992189262050484 6.25481860001796 >50 0.022968952977585166 1.1963516090597086 >100 0.01316886617877714 1.7700935862061729 >500 3.062527018320257E-4 0.1432092586999624 >1k 9.187581054960771E-4 1.3320949687604717 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2494 0.5641244873908903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1975 0.44673049823456634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1419 0.32096738075688586 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 633 0.14317995209239517 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 493 0.1115129800656411 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.2619265733395762E-4 14-15 0.0 0.0 0.0 2.2619265733395762E-4 3.392889860009364E-4 16-17 0.0 0.0 0.0 2.2619265733395762E-4 4.5238531466791523E-4 18-19 0.0 0.0 0.0 2.2619265733395762E-4 4.5238531466791523E-4 20-21 0.0 0.0 0.0 2.2619265733395762E-4 4.5238531466791523E-4 22-23 0.0 0.0 0.0 5.654816433348941E-4 4.5238531466791523E-4 24-25 0.0 0.0 0.0 0.0012440596153367668 4.5238531466791523E-4 26-27 0.0 0.0 0.0 0.0021488302446725973 4.5238531466791523E-4 28-29 0.0 0.0 0.0 0.0033928898600093646 4.5238531466791523E-4 30-31 0.0 0.0 0.0 0.006559587062684771 5.654816433348941E-4 32-33 0.0 0.0 0.0 0.011988210838699755 6.785779720018728E-4 34-35 0.0 0.0 0.0 0.016738256642712862 6.785779720018728E-4 36-37 0.0 0.0 0.0 0.02024424283138921 6.785779720018728E-4 38-39 0.0 0.0 0.0 0.026238348250739084 6.785779720018728E-4 40-41 0.0 0.0 0.0 0.03426818758609458 6.785779720018728E-4 42-43 0.0 0.0 0.0 0.05078025157147349 6.785779720018728E-4 44-45 0.0 0.0 0.0 0.07068520541686175 6.785779720018728E-4 46-47 0.0 0.0 0.0 0.08776275104557556 6.785779720018728E-4 48-49 0.0 0.0 0.0 0.11569754422631932 6.785779720018728E-4 50-51 0.0 0.0 0.0 0.15154908041375162 6.785779720018728E-4 52-53 0.0 0.0 0.0 0.22336524911728317 9.047706293358305E-4 54-55 0.0 0.0 0.0 0.28183605103811116 9.047706293358305E-4 56-57 0.0 0.0 0.0 0.37649767813237245 9.047706293358305E-4 58-59 0.0 0.0 0.0 0.4839391903660023 9.047706293358305E-4 60-61 0.0 0.0 0.0 0.6006546015503245 9.047706293358305E-4 62-63 0.0 0.0 0.0 0.7632871221734401 0.0015833486013377034 64-65 0.0 0.0 0.0 0.8517284511910175 0.0015833486013377034 66-67 0.0 0.0 0.0 1.040712416393539 0.0015833486013377034 68-69 0.0 0.0 0.0 1.2940481926075715 0.0015833486013377034 70-71 0.0 0.0 0.0 1.4871036256421044 0.0015833486013377034 72-73 0.0 0.0 0.0 1.8118031852450005 0.0015833486013377034 74-75 0.0 0.0 0.0 2.133901529288556 0.0015833486013377034 76-77 0.0 0.0 0.0 2.340189232777125 0.001809541258671661 78-79 0.0 0.0 0.0 2.5753165000757745 0.001809541258671661 80-81 0.0 0.0 0.0 2.9978443839756075 0.001809541258671661 82-83 0.0 0.0 0.0 3.3383774295918807 0.001809541258671661 84-85 0.0 0.0 0.0 3.8182451521258716 0.001809541258671661 86-87 0.0 0.0 0.0 4.442989271682263 0.001809541258671661 88-89 0.0 0.0 0.0 5.270515108538547 0.001809541258671661 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1180 0.0 51.175095 1 GTATCAA 2020 0.0 38.603745 1 TGGTATC 580 0.0 37.689507 2 GTGGTAT 630 0.0 35.472702 1 GTATGGT 120 1.4888428E-7 31.699009 1 ACGCACT 60 0.003946145 31.670258 8 TATCAAC 2745 0.0 28.391745 2 ATCAACG 2750 0.0 28.340122 3 TCAACGC 2745 0.0 28.218624 4 CAACGCA 2775 0.0 27.910393 5 AACGCAG 2890 0.0 26.793694 6 GTACATG 2310 0.0 25.112202 1 TACATGG 2285 0.0 24.74864 2 TCTAGAC 180 1.9368599E-7 23.760775 3 GTAATAC 100 0.001647679 23.760775 3 ACATGGG 2320 0.0 23.760775 3 TGGATCG 105 0.002189757 22.626743 5 CGCAGAG 3420 0.0 22.50255 8 CATGGGG 995 0.0 22.447365 4 ACGCAGA 3435 0.0 22.404287 7 >>END_MODULE