FastQCFastQC Report
Thu 26 May 2016
SRR937211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442101
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40730.9212826933212095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.7652097597607787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24160.5464814601188416No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.20515674020189956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.16828733705646448No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG4530.10246527377228279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.060.886641
GTGTACG554.942711E-543.2049141
TGTACGC459.620105E-442.2209132
GTATCAA24950.041.52521
CGTAAGC704.305084E-640.7130243
GTAGGAC702.0516696E-433.927523
GTGGTAT5050.033.8794981
ATCAACG33450.030.9556943
TGGTATC5550.030.8098582
TCAACGC34150.030.3211694
CAACGCA34450.030.0571255
TATCAAC34300.030.0500892
AACGCAG35400.029.3846846
GCGGTTA650.00583788829.2331739
CAGTCCG650.00583788829.2331739
CCTTAGA1004.9473532E-528.4991192
CGGTTAC350.00838718227.14508610-11
TCGTAAG1056.897838E-527.1420172
ACGCAGA40600.025.6240277
CGCAGAG40650.025.5925088
Produced by FastQC (version 0.11.5)