##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937211_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 442101 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28042913270949 33.0 31.0 34.0 31.0 34.0 2 32.46986322130011 34.0 31.0 34.0 31.0 34.0 3 32.51866428711991 34.0 31.0 34.0 31.0 34.0 4 36.14992275520752 37.0 35.0 37.0 35.0 37.0 5 36.09333840004886 37.0 35.0 37.0 35.0 37.0 6 36.137678946666036 37.0 35.0 37.0 35.0 37.0 7 36.080990542885 37.0 35.0 37.0 35.0 37.0 8 36.056699713413906 37.0 35.0 37.0 35.0 37.0 9 37.764843327655896 39.0 37.0 39.0 35.0 39.0 10-11 37.692632452765324 39.0 37.0 39.0 35.0 39.0 12-13 37.583155206615686 39.0 37.0 39.0 35.0 39.0 14-15 38.80216398515272 40.0 38.0 41.0 35.0 41.0 16-17 38.75459227642553 40.0 38.0 41.0 35.0 41.0 18-19 38.765961850346415 40.0 38.0 41.0 35.0 41.0 20-21 38.69221286538597 40.0 38.0 41.0 34.0 41.0 22-23 38.62941725985691 40.0 38.0 41.0 34.0 41.0 24-25 38.646053729803825 40.0 38.0 41.0 34.0 41.0 26-27 38.493479996652354 40.0 38.0 41.0 34.0 41.0 28-29 38.33752694520031 40.0 38.0 41.0 34.0 41.0 30-31 38.31693210375005 40.0 38.0 41.0 34.0 41.0 32-33 38.16309847749723 40.0 37.0 41.0 34.0 41.0 34-35 38.00790882626368 40.0 37.0 41.0 33.5 41.0 36-37 37.82239352546138 40.0 37.0 41.0 33.0 41.0 38-39 37.70005157192587 40.0 37.0 41.0 33.0 41.0 40-41 37.47961099386792 40.0 36.5 41.0 33.0 41.0 42-43 37.245525343756285 40.0 36.0 41.0 32.5 41.0 44-45 36.879399729925964 40.0 36.0 41.0 31.5 41.0 46-47 36.41103729690727 39.0 35.5 41.0 30.5 41.0 48-49 35.995438825064866 39.0 35.0 41.0 29.5 41.0 50-51 35.70435828012151 39.0 35.0 40.5 28.0 41.0 52-53 35.45927061915717 39.0 35.0 40.0 28.0 41.0 54-55 35.235391912707726 38.5 35.0 40.0 27.0 41.0 56-57 34.92669887650107 38.0 34.5 40.0 26.5 41.0 58-59 34.65691323928243 38.0 34.0 40.0 26.0 41.0 60-61 34.30842047405457 38.0 34.0 40.0 26.0 41.0 62-63 33.91751545461331 37.0 34.0 40.0 24.5 41.0 64-65 33.499601900923096 36.5 33.0 39.0 23.0 41.0 66-67 33.064487526605916 36.0 33.0 39.0 22.0 40.0 68-69 32.60923974385943 35.5 32.0 39.0 20.5 40.0 70-71 32.15194039371094 35.0 32.0 38.0 19.5 40.0 72-73 31.68659084688793 35.0 31.0 37.5 18.0 39.5 74-75 31.21286312403727 35.0 31.0 37.0 15.5 39.0 76-77 29.90991651229018 33.5 29.5 35.5 13.0 38.0 78-79 30.561202078258134 34.0 30.5 36.0 11.5 38.0 80-81 30.383563936747485 34.0 31.0 35.5 7.5 37.0 82-83 29.99116378384125 34.0 30.0 35.0 6.0 37.0 84-85 29.580976971325555 34.0 30.0 35.0 2.0 36.5 86-87 29.22076403355794 34.0 29.0 35.0 2.0 36.0 88-89 28.77699439720788 34.0 29.0 35.0 2.0 36.0 90-91 28.23003906347192 34.0 28.5 35.0 2.0 35.0 92-93 27.74901323453238 33.0 27.0 35.0 2.0 35.0 94-95 27.269850102125986 33.0 26.5 35.0 2.0 35.0 96-97 26.63855205032334 33.0 25.5 35.0 2.0 35.0 98-99 25.71245484629078 32.0 24.0 34.0 2.0 35.0 100-101 22.815709532437157 29.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28871700290721947 1101 2 0.21738992529164136 1101 3 0.3285313428411385 1101 4 0.07635984579891186 1101 5 0.053317950151438254 1101 6 0.20129928611911652 1101 7 0.13328529988267235 1101 8 0.03869341970922591 1101 9 0.23821134695532464 1101 10-11 0.13158574534374878 1101 12-13 0.2392787632507165 1101 14-15 0.2979869789557483 1101 16-17 0.23243097994520667 1101 18-19 0.30617948116682214 1101 20-21 0.3691438659406714 1101 22-23 0.20206944378376335 1101 24-25 0.005628304548260132 1101 26-27 0.0942452389253674 1101 28-29 -0.07420808855595595 1101 30-31 0.08093057812671134 1101 32-33 0.2593294960711887 1101 34-35 0.07519528909494255 1101 36-37 0.21404279328158538 1101 38-39 0.3228286352363625 1101 40-41 0.14139820154152716 1101 42-43 0.36780795048856163 1101 44-45 0.2555140816362851 1101 46-47 0.46947219548013663 1101 48-49 0.4182582001313122 1101 50-51 0.49032538942427095 1101 52-53 0.48148856108170435 1101 54-55 0.33655410908929895 1101 56-57 0.5019532000922879 1101 58-59 0.5859624751404766 1101 60-61 0.42228863870710853 1101 62-63 0.7634718522857398 1101 64-65 0.8813338017890047 1101 66-67 0.6451349211597233 1101 68-69 0.7881257295745385 1101 70-71 0.6603188981785273 1101 72-73 0.779209369719343 1101 74-75 0.7058317426077068 1101 76-77 0.6448721463629994 1101 78-79 0.9408304328025139 1101 80-81 0.6855939498011558 1101 82-83 0.5735622215933276 1101 84-85 0.8539940955952758 1101 86-87 0.9700361239263913 1101 88-89 1.13609823946733 1101 90-91 0.8949127980679918 1101 92-93 0.6310803746296507 1101 94-95 0.7092171886583323 1101 96-97 1.301242637620188 1101 98-99 1.5801362843482032 1101 100-101 2.12980693517947 1102 1 0.11354413121894424 1102 2 0.08099388030916543 1102 3 0.036990266717239706 1102 4 0.12987556363610508 1102 5 0.11667594261412262 1102 6 0.18018121277550136 1102 7 -0.0032427802135970296 1102 8 0.004179440963106629 1102 9 -0.09108799928224443 1102 10-11 0.10373388119656113 1102 12-13 0.0814345571401347 1102 14-15 0.041393711237581954 1102 16-17 0.11305048869913037 1102 18-19 0.0774644846185879 1102 20-21 -0.05833490469924385 1102 22-23 0.020142032916886876 1102 24-25 -0.19863567514096303 1102 26-27 -0.08982679943272842 1102 28-29 -0.24895844788009214 1102 30-31 0.05796212697404712 1102 32-33 -0.0306705416531301 1102 34-35 -0.09604859484996808 1102 36-37 0.04062598860678435 1102 38-39 -0.026445737502534428 1102 40-41 -0.02306473620202354 1102 42-43 -0.06278684883692165 1102 44-45 0.08142389788113036 1102 46-47 0.35174591558833157 1102 48-49 0.14483045540422523 1102 50-51 0.6144930966533764 1102 52-53 0.3915066310301256 1102 54-55 0.1094989825430801 1102 56-57 0.41888236513995025 1102 58-59 0.26918571633845545 1102 60-61 0.4098226377489169 1102 62-63 0.6298202514542908 1102 64-65 0.5987729996191007 1102 66-67 0.5856285815880469 1102 68-69 0.680558608581336 1102 70-71 0.6188070584012557 1102 72-73 0.880897155716827 1102 74-75 0.4951602874675771 1102 76-77 0.19315738747797795 1102 78-79 0.4933615459339755 1102 80-81 0.23054247496893865 1102 82-83 0.3611441305192784 1102 84-85 0.6464895969703264 1102 86-87 0.6385280188566185 1102 88-89 0.6574068206179966 1102 90-91 0.884608400544213 1102 92-93 0.8871134362221405 1102 94-95 1.0150520089585449 1102 96-97 1.1392412116409574 1102 98-99 1.734813533943612 1102 100-101 2.7547668530913576 1103 1 0.2971696508285575 1103 2 0.22753885289787235 1103 3 0.28181998967817634 1103 4 0.2004305884654798 1103 5 0.18702011136783625 1103 6 0.2229032356168048 1103 7 0.37509756731164146 1103 8 0.33834759222867206 1103 9 0.4682400153141586 1103 10-11 0.37711093489171077 1103 12-13 0.43893457865605257 1103 14-15 0.46687975600791987 1103 16-17 0.3835271955575479 1103 18-19 0.5368940543861385 1103 20-21 0.49315916120833947 1103 22-23 0.6142612999983825 1103 24-25 0.464420619128056 1103 26-27 0.40122257531446337 1103 28-29 0.4124023914131527 1103 30-31 0.21301449673814687 1103 32-33 0.2947685983983348 1103 34-35 0.1520063654316388 1103 36-37 0.43271501290256964 1103 38-39 0.32160879246054463 1103 40-41 0.19649237997537483 1103 42-43 0.45106940756407 1103 44-45 0.3835914122752868 1103 46-47 0.4081391578662661 1103 48-49 0.08201901237409714 1103 50-51 0.373637365240981 1103 52-53 0.3210936113129321 1103 54-55 0.20780524884138885 1103 56-57 0.27030628912849153 1103 58-59 0.428268019058919 1103 60-61 0.6632630343304626 1103 62-63 0.26615591711401265 1103 64-65 0.1255775403401458 1103 66-67 0.35224533130619307 1103 68-69 0.22112473562857105 1103 70-71 0.02709112026384375 1103 72-73 -0.09956208020960489 1103 74-75 0.3879292150277607 1103 76-77 0.3009134485143612 1103 78-79 0.32309096164748397 1103 80-81 0.416166824112171 1103 82-83 0.4356645405267656 1103 84-85 0.46349449399510334 1103 86-87 0.6086402459270985 1103 88-89 0.575598966444339 1103 90-91 0.7729428957375539 1103 92-93 1.1192207920869528 1103 94-95 1.1841693396066866 1103 96-97 1.4603212250668989 1103 98-99 1.3892247291102713 1103 100-101 2.083095582016508 1104 1 0.1366708575864024 1104 2 0.1335458252765278 1104 3 0.1238496414423409 1104 4 0.10593953379743226 1104 5 -0.041890358693081 1104 6 0.04655377386911397 1104 7 -0.0359184078636261 1104 8 0.043391073820828296 1104 9 0.034688316275733655 1104 10-11 0.10469492906862854 1104 12-13 0.05277685732134074 1104 14-15 0.20377781370243753 1104 16-17 0.14097365765002934 1104 18-19 0.14243747851362798 1104 20-21 0.3680250770034661 1104 22-23 0.17739101688916747 1104 24-25 0.12865940394205921 1104 26-27 0.0774571077436832 1104 28-29 0.10043633387187612 1104 30-31 0.23321811318069763 1104 32-33 0.2581540330227057 1104 34-35 0.0633336075110904 1104 36-37 0.13046527149354148 1104 38-39 0.2381701072519462 1104 40-41 0.30508143104229646 1104 42-43 0.022335162458141156 1104 44-45 -0.2603000944480769 1104 46-47 -0.19297469877856344 1104 48-49 -0.33363723403436296 1104 50-51 -0.26191218845016095 1104 52-53 -0.19661508117451376 1104 54-55 -0.14663676931744618 1104 56-57 -0.12999252709953169 1104 58-59 -0.21021223225318408 1104 60-61 -0.12451997911626478 1104 62-63 -0.04216113456586612 1104 64-65 -0.17914797013757777 1104 66-67 -0.0026228830753041166 1104 68-69 0.08827602092144815 1104 70-71 -0.20306920613541735 1104 72-73 -0.303140212991277 1104 74-75 -0.14433240241084988 1104 76-77 -0.2636768284652966 1104 78-79 -0.3529807542472412 1104 80-81 -0.32761741524728905 1104 82-83 -0.5102755812983126 1104 84-85 -0.13352500567926384 1104 86-87 -0.09003423593322424 1104 88-89 -0.24651918089504576 1104 90-91 -0.052287920643703245 1104 92-93 -0.0035668185325157253 1104 94-95 0.11211344564813785 1104 96-97 0.3143322582814676 1104 98-99 0.4338604031707298 1104 100-101 0.9903907205063369 1105 1 0.10953501058727966 1105 2 0.12341706631163163 1105 3 0.14767214699952547 1105 4 0.1557864490346148 1105 5 0.19443686523008807 1105 6 0.0408183378920981 1105 7 0.123635758977791 1105 8 0.15284667923960882 1105 9 0.17709908673247554 1105 10-11 -0.03764768721288192 1105 12-13 0.1494247204258201 1105 14-15 0.3183292543768417 1105 16-17 0.4023973459442871 1105 18-19 0.336236497093509 1105 20-21 0.47836668670022675 1105 22-23 0.4238312718906272 1105 24-25 0.35765219008474247 1105 26-27 0.09229374742829322 1105 28-29 0.44312601218416603 1105 30-31 0.21071150746833212 1105 32-33 0.13434410977479416 1105 34-35 0.21996763007536657 1105 36-37 0.173313277197515 1105 38-39 0.36697101317820824 1105 40-41 0.262437887864003 1105 42-43 -0.09312849563857384 1105 44-45 0.10478609437044639 1105 46-47 0.17990727916045302 1105 48-49 0.1341363263397497 1105 50-51 0.19336818734147698 1105 52-53 0.299725563878539 1105 54-55 0.4226602990044981 1105 56-57 0.6216326878482548 1105 58-59 0.37762277173121106 1105 60-61 0.5381168534970797 1105 62-63 0.5636980274523324 1105 64-65 0.6612559219900902 1105 66-67 1.0451322232452114 1105 68-69 0.8912894839923737 1105 70-71 0.6476364517096549 1105 72-73 0.5495628864509037 1105 74-75 0.6644007687504967 1105 76-77 0.7019105556726011 1105 78-79 0.9538074016056299 1105 80-81 0.9949300829702175 1105 82-83 1.0890774782998918 1105 84-85 1.062046021456581 1105 86-87 1.351049100895196 1105 88-89 1.2749126859002367 1105 90-91 1.2586838530424949 1105 92-93 1.1417326404281845 1105 94-95 1.4418066470070308 1105 96-97 1.5481220498345465 1105 98-99 1.851787048307667 1105 100-101 2.408663379148283 1106 1 -0.006179631468093305 1106 2 0.026023701382726472 1106 3 0.08523762467475393 1106 4 0.1214262594611526 1106 5 0.12061546338424023 1106 6 -0.07978530391383742 1106 7 0.0845985926021342 1106 8 0.004592750579092808 1106 9 -0.02732845130693562 1106 10-11 -0.23600139137850817 1106 12-13 -0.4567364170149446 1106 14-15 -0.21985982020534323 1106 16-17 -0.05700025944359055 1106 18-19 -0.28893187407138043 1106 20-21 0.02685134621631846 1106 22-23 -0.14051769293850214 1106 24-25 -0.09206719130711605 1106 26-27 0.10252697267149813 1106 28-29 -0.1446265445631525 1106 30-31 -0.22518896646531772 1106 32-33 -0.047621467675938334 1106 34-35 -0.38866918708602327 1106 36-37 0.14835069301194181 1106 38-39 0.07198473231016322 1106 40-41 -0.1801537808813194 1106 42-43 0.027225707404575417 1106 44-45 0.30722435827665606 1106 46-47 -0.1070408375768892 1106 48-49 -0.1658449657530383 1106 50-51 0.19916140260713178 1106 52-53 0.211000195956359 1106 54-55 0.45621604856797404 1106 56-57 0.46118120368761595 1106 58-59 0.5454701152083885 1106 60-61 0.5881605052805696 1106 62-63 0.5862971793605354 1106 64-65 0.5465390349858552 1106 66-67 0.709393884507378 1106 68-69 0.5811497982852103 1106 70-71 0.36849576824743835 1106 72-73 0.3350642583641026 1106 74-75 0.6001016916739204 1106 76-77 0.47039396799487676 1106 78-79 0.21574304640483177 1106 80-81 0.23521444315979068 1106 82-83 0.25926331017816295 1106 84-85 0.6908811424343817 1106 86-87 0.7847794575926272 1106 88-89 0.7550760682898634 1106 90-91 0.7852179014336151 1106 92-93 1.5415892131395843 1106 94-95 1.0187273254804659 1106 96-97 1.1300751553471429 1106 98-99 1.5993238405251766 1106 100-101 1.766776374907824 1107 1 -0.14732335616444914 1107 2 -0.05378399092496977 1107 3 0.3105412683994544 1107 4 -0.0684775866926941 1107 5 -0.20324587264815364 1107 6 -0.18542801646444218 1107 7 -0.20323035476628348 1107 8 -0.07288700650592972 1107 9 -0.014069341995195828 1107 10-11 -0.06353175575097936 1107 12-13 -0.01587103239955212 1107 14-15 -0.13033552870736997 1107 16-17 -0.1285336602533036 1107 18-19 -0.17717833156125096 1107 20-21 -0.18323215580797125 1107 22-23 -0.2274230573109719 1107 24-25 -0.13561476215732426 1107 26-27 -0.23140521356332044 1107 28-29 -0.1641356538748937 1107 30-31 -0.236967812619163 1107 32-33 -0.34304150816176815 1107 34-35 -0.1923888227135464 1107 36-37 -0.1161735984860357 1107 38-39 -0.26922225149549917 1107 40-41 -0.1304827282497385 1107 42-43 0.06182844019809863 1107 44-45 0.10647790078677843 1107 46-47 0.27896624744335696 1107 48-49 -0.10916480380971905 1107 50-51 0.03941949977311765 1107 52-53 0.2936418963612226 1107 54-55 0.33363254654369 1107 56-57 0.16645701340421226 1107 58-59 0.08445037836628444 1107 60-61 0.49004562673805907 1107 62-63 0.4636251145832091 1107 64-65 0.11503599854860624 1107 66-67 0.03772131365717257 1107 68-69 0.17756933269816955 1107 70-71 0.23466479658751993 1107 72-73 0.20409259844507233 1107 74-75 0.1103370155605603 1107 76-77 0.22904781414872488 1107 78-79 0.10388828931981209 1107 80-81 0.26598367392902134 1107 82-83 0.37605227778950123 1107 84-85 0.5422854946611011 1107 86-87 0.5793518260136779 1107 88-89 0.5985603800307508 1107 90-91 0.8224142577089175 1107 92-93 1.1913361767023396 1107 94-95 0.8098457465330959 1107 96-97 1.0727244468451183 1107 98-99 1.3519807231162666 1107 100-101 1.6154226299685526 1108 1 -0.1389515612926573 1108 2 -0.03197629861727336 1108 3 -0.011253603395417144 1108 4 -0.09124040720551108 1108 5 -0.052893308545584716 1108 6 -0.17010109338752244 1108 7 -0.08698035476628263 1108 8 0.09304889092997115 1108 9 -0.08188985481570654 1108 10-11 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0.4959273386776957 2316 48-49 0.5266580942224763 2316 50-51 0.07489579673197255 2316 52-53 0.25989860476909143 2316 54-55 0.8216077254345677 2316 56-57 0.6184639454256882 2316 58-59 0.3573428202267479 2316 60-61 0.8187296607273211 2316 62-63 0.9451099088587043 2316 64-65 1.1503334046308993 2316 66-67 1.3620560632099483 2316 68-69 1.4491424543439635 2316 70-71 1.2687099665338586 2316 72-73 1.32775091687083 2316 74-75 1.3903403697644947 2316 76-77 1.2355102470646457 2316 78-79 1.2222301945076488 2316 80-81 1.575912117578394 2316 82-83 1.4293673493458527 2316 84-85 1.4848223909043945 2316 86-87 1.464308221362515 2316 88-89 1.7853392261846004 2316 90-91 1.8675618426820826 2316 92-93 1.6296859083660244 2316 94-95 1.7457469093237954 2316 96-97 1.9590225237681977 2316 98-99 1.8426224941359486 2316 100-101 2.482657647857639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 9.0 6 16.0 7 35.0 8 111.0 9 207.0 10 364.0 11 579.0 12 975.0 13 1525.0 14 1892.0 15 2790.0 16 3912.0 17 5766.0 18 5350.0 19 3137.0 20 2512.0 21 2509.0 22 2616.0 23 2797.0 24 3109.0 25 3641.0 26 4328.0 27 5185.0 28 6489.0 29 7939.0 30 10103.0 31 12598.0 32 16267.0 33 21266.0 34 30386.0 35 45017.0 36 67884.0 37 96666.0 38 68752.0 39 5369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.800601905279116 19.134930871404972 15.248342497680628 21.81612472563528 2 15.863795829459784 20.41366113173234 40.34869860054603 23.373844438261845 3 19.564986281415333 24.52154598157435 30.818070983779727 25.0953967532306 4 12.719944085175108 16.990235262982893 36.84226002655502 33.44756062528698 5 13.02691698710699 37.87084370052025 34.0798461886451 15.022393123727666 6 31.730532163464908 36.72577080802803 16.783495174179656 14.760201854327404 7 28.150128590525696 33.807659335762644 20.00877627510456 18.033435798607105 8 27.248750851049873 34.73618019411854 19.074374407657977 18.94069454717361 9 26.722174344776423 16.28360940147161 19.965121092239105 37.02909516151286 10-11 25.14131386266939 27.079671839692736 26.60003030981608 21.178983987821788 12-13 26.389716735187836 24.384864644773824 27.829170224677803 21.396248395360544 14-15 22.944756967299327 25.428465441154852 26.826449159807375 24.80032843173845 16-17 21.527546872773414 28.62388911131167 27.807220522007412 22.0413434939075 18-19 21.36502744847897 26.030251005991843 31.55613762466043 21.04858392086876 20-21 22.00706176670836 25.032515341586386 32.29966591193375 20.6607569797715 22-23 21.48657421335137 25.265722228379744 32.25276353379462 20.994940024474268 24-25 21.464552217705908 25.321137025249886 32.33706777410592 20.877242982938288 26-27 21.12429468626899 25.73941308397262 32.03529594053706 21.100996289221325 28-29 21.01760628293332 25.872312318969254 32.149703734450156 20.96037766364727 30-31 21.12459101308871 25.671880813891022 31.95577456155133 21.247753611468937 32-33 21.017303777425923 25.74598507125085 31.964035286134358 21.272675865188873 34-35 21.42858758518981 25.6289852318814 32.01259440716036 20.929832775768435 36-37 21.42001648946337 25.495334205684 31.849206232519528 21.2354430723331 38-39 21.19495454087928 25.694270872799287 32.01839853155561 21.092376054765825 40-41 21.325669926102858 25.739367248660372 31.928111449646124 21.006851375590646 42-43 21.291405484763416 25.678243500331405 31.615320057639103 21.41503095726608 44-45 21.35337852662627 26.101727885709376 31.426981617322742 21.11791197034162 46-47 21.645393247244407 26.579220585341357 30.65871825668795 21.116667910726285 48-49 21.70442953080857 26.835836155086735 30.1091831956951 21.350551118409594 50-51 21.852472625033645 27.039635739344632 29.947003060386653 21.16088857523507 52-53 22.04665902135485 26.878812759980185 29.71244127473134 21.362086943933626 54-55 21.68848294846653 27.08453498182542 29.834585309691676 21.39239676001638 56-57 21.676381641299162 27.315251492306057 29.60590453312705 21.40246233326774 58-59 21.65274450860776 26.790597623619945 29.987039160734763 21.569618707037534 60-61 21.57640448675755 26.761758059809864 30.354376036245114 21.307461417187476 62-63 21.44905802067853 27.0287784917926 30.35629867383245 21.165864813696416 64-65 21.390700315086374 26.907765420118935 30.111784411254444 21.589749853540255 66-67 21.263240752678687 27.29941800629268 29.88977631808116 21.547564922947473 68-69 21.623000174168347 26.818758609458023 30.186427988174646 21.371813228198985 70-71 21.450075887636537 27.119029362068847 29.84012702979636 21.590767720498256 72-73 21.397421397873785 27.101108346527937 29.640805247681516 21.86066500791676 74-75 21.350324925752258 27.13882121958557 29.549130176136224 21.96172367852595 76-77 21.39454559026105 26.95526587815906 29.75553097595346 21.89465755562643 78-79 21.46489150669191 27.024933216617924 29.59968423505036 21.91049104163981 80-81 21.32374728851552 27.475848279013164 29.383896439953766 21.816507992517547 82-83 21.339612484109857 27.370175843365047 29.43295377947876 21.857257893046338 84-85 21.099703461426238 27.24106030070052 29.430039742049896 22.229196495823352 86-87 21.22444871194591 27.28935243304132 29.28425857439816 22.20194028061461 88-89 21.09133433310488 27.082386151580746 29.367158183311055 22.45912133200332 90-91 21.050821845294916 26.719246969827214 29.641853710180204 22.588077474697663 92-93 20.828559634013246 26.40047048977307 30.098565345487653 22.672404530726038 94-95 20.6645087887157 26.56157755806931 30.034539618774897 22.739374034440093 96-97 20.657949201653018 26.310051323113946 30.014069183286175 23.01793029194686 98-99 20.441236012748277 26.685086372056936 29.753969133610347 23.119708481584443 100-101 20.37105323285954 26.63396216170971 30.03151305654026 22.96347154889048 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 211.0 1 176.0 2 226.0 3 311.5 4 219.5 5 158.0 6 386.5 7 956.0 8 1549.5 9 1727.5 10 1388.0 11 1019.0 12 928.0 13 944.0 14 969.5 15 986.5 16 1037.5 17 1117.0 18 1172.5 19 1233.0 20 1373.5 21 1513.5 22 1713.5 23 1900.5 24 2142.5 25 2575.5 26 3102.0 27 3749.5 28 4373.0 29 5039.5 30 5738.5 31 6580.0 32 7613.5 33 8326.5 34 8891.5 35 9500.5 36 10318.0 37 11260.5 38 12002.5 39 12313.0 40 12640.0 41 13517.0 42 14072.0 43 14336.5 44 14811.5 45 15394.0 46 15964.5 47 16330.0 48 16626.5 49 16268.5 50 15810.5 51 15352.5 52 14610.0 53 14570.0 54 14258.0 55 12980.5 56 11668.5 57 10555.5 58 9532.0 59 8358.0 60 6809.5 61 5511.5 62 4479.5 63 3186.0 64 2095.0 65 1508.0 66 1112.5 67 890.0 68 645.0 69 427.5 70 309.5 71 207.0 72 163.5 73 128.0 74 100.0 75 87.5 76 62.0 77 36.0 78 19.5 79 13.0 80 5.0 81 2.5 82 3.0 83 1.5 84 1.0 85 1.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038678944404106755 2 0.0 3 0.0 4 0.0 5 2.2619265733395764E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005315527447348005 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.523853146679153E-4 22-23 9.047706293358306E-4 24-25 0.0 26-27 0.002375022902006555 28-29 0.0032797935313423855 30-31 5.654816433348941E-4 32-33 2.2619265733395764E-4 34-35 0.0 36-37 1.1309632866697882E-4 38-39 1.1309632866697882E-4 40-41 0.0 42-43 0.009047706293358306 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.2619265733395764E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.785779720018728E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.01063105489469601 92-93 0.00192263758733864 94-95 0.0 96-97 0.0 98-99 4.523853146679153E-4 100-101 0.014023944754705373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 442101.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.06505462101131 #Duplication Level Percentage of deduplicated Percentage of total 1 85.74195642446817 60.07514860192734 2 8.806654720688583 12.340774880668654 3 2.3823295347558164 5.007541469337442 4 1.0116832144636572 2.8353455872222577 5 0.5523431422875474 1.9349976216959017 6 0.3440242320888192 1.4462445967352755 7 0.22350033600552383 1.0961694275028986 8 0.16253857736113442 0.9110619440663483 9 0.12106355210856631 0.7634091951990314 >10 0.6047419805245041 7.238742971065856 >50 0.0293032164331573 1.4045700427563386 >100 0.01823311235431805 2.317301029324979 >500 6.511825840827875E-4 0.37663555812320737 >1k 9.767738761241812E-4 2.252057074374502 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4073 0.9212826933212095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3383 0.7652097597607787 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2416 0.5464814601188416 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 907 0.20515674020189956 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 744 0.16828733705646448 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 453 0.10246527377228279 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2619265733395762E-4 0.0 16-17 0.0 0.0 0.0 3.392889860009364E-4 0.0 18-19 0.0 0.0 0.0 4.5238531466791523E-4 0.0 20-21 0.0 0.0 0.0 4.5238531466791523E-4 0.0 22-23 0.0 0.0 0.0 7.916743006688516E-4 0.0 24-25 0.0 0.0 0.0 0.0013571559440037457 0.0 26-27 0.0 0.0 0.0 0.0021488302446725973 0.0 28-29 1.1309632866697881E-4 0.0 0.0 0.0033928898600093646 0.0 30-31 2.2619265733395762E-4 0.0 0.0 0.006559587062684771 0.0 32-33 2.2619265733395762E-4 0.0 0.0 0.011648921852698817 0.0 34-35 2.2619265733395762E-4 0.0 0.0 0.016625160314045886 0.0 36-37 2.2619265733395762E-4 0.0 0.0 0.02024424283138921 0.0 38-39 2.2619265733395762E-4 0.0 0.0 0.026012155593405126 0.0 40-41 2.2619265733395762E-4 0.0 0.0 0.03392889860009364 0.0 42-43 3.392889860009364E-4 0.0 0.0 0.05089334790014047 0.0 44-45 4.5238531466791523E-4 0.0 0.0 0.07091139807419572 0.0 46-47 5.654816433348941E-4 0.0 0.0 0.08787584737424253 0.0 48-49 6.785779720018728E-4 0.0 0.0 0.11547135156898536 0.0 50-51 6.785779720018728E-4 0.0 0.0 0.15087050244174974 0.0 52-53 6.785779720018728E-4 0.0 0.0 0.2224604784879473 0.0 54-55 6.785779720018728E-4 0.0 0.0 0.28217534002411215 0.0 56-57 6.785779720018728E-4 0.0 0.0 0.37717625610437433 0.0 58-59 6.785779720018728E-4 0.0 0.0 0.48699279124001077 0.0 60-61 6.785779720018728E-4 0.0 0.0 0.6052915510256707 0.0 62-63 6.785779720018728E-4 0.0 0.0 0.7695074202501239 2.2619265733395762E-4 64-65 6.785779720018728E-4 0.0 0.0 0.8576094602817004 2.2619265733395762E-4 66-67 6.785779720018728E-4 0.0 0.0 1.0481767740855596 2.2619265733395762E-4 68-69 6.785779720018728E-4 0.0 0.0 1.3053578254742693 2.2619265733395762E-4 70-71 6.785779720018728E-4 0.0 0.0 1.500901377739476 2.2619265733395762E-4 72-73 6.785779720018728E-4 0.0 0.0 1.8282021529017125 2.2619265733395762E-4 74-75 7.916743006688516E-4 0.0 0.0 2.155729120721283 3.392889860009364E-4 76-77 9.047706293358305E-4 0.0 0.0 2.3643918471118592 4.5238531466791523E-4 78-79 9.047706293358305E-4 0.0 0.0 2.6016679446551807 4.5238531466791523E-4 80-81 9.047706293358305E-4 0.0 0.0 3.031999475233035 4.5238531466791523E-4 82-83 9.047706293358305E-4 0.0 0.0 3.3776218556393225 4.5238531466791523E-4 84-85 9.047706293358305E-4 0.0 0.0 3.8668765734526724 4.5238531466791523E-4 86-87 9.047706293358305E-4 0.0 0.0 4.503948192833764 5.654816433348941E-4 88-89 9.047706293358305E-4 0.0 0.0 5.3525099468221065 6.785779720018728E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1405 0.0 60.88664 1 GTGTACG 55 4.942711E-5 43.204914 1 TGTACGC 45 9.620105E-4 42.220913 2 GTATCAA 2495 0.0 41.5252 1 CGTAAGC 70 4.305084E-6 40.713024 3 GTAGGAC 70 2.0516696E-4 33.92752 3 GTGGTAT 505 0.0 33.879498 1 ATCAACG 3345 0.0 30.955694 3 TGGTATC 555 0.0 30.809858 2 TCAACGC 3415 0.0 30.321169 4 CAACGCA 3445 0.0 30.057125 5 TATCAAC 3430 0.0 30.050089 2 AACGCAG 3540 0.0 29.384684 6 GCGGTTA 65 0.005837888 29.233173 9 CAGTCCG 65 0.005837888 29.233173 9 CCTTAGA 100 4.9473532E-5 28.499119 2 CGGTTAC 35 0.008387182 27.145086 10-11 TCGTAAG 105 6.897838E-5 27.142017 2 ACGCAGA 4060 0.0 25.624027 7 CGCAGAG 4065 0.0 25.592508 8 >>END_MODULE