FastQCFastQC Report
Thu 26 May 2016
SRR937210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401829
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.5995087462577365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.4653720861361425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12810.31879232210716496No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5480.13637641882492305No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.12467990115198255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.054.4718631
GTATCAA19850.040.9666061
TATCAAC24750.032.8519442
GTGGTAT4900.032.0267031
ATCAACG25450.031.948353
TCAACGC25800.031.3306474
CAACGCA26550.030.4456025
TGGTATC4850.030.3920572
AACGCAG27550.029.3112326
TGTATCG650.00581032229.260845
CTAGTAA650.00581032229.260844
GGTCTAG1004.9064503E-528.5328791
TAGGGTC1056.846706E-527.170785
GTAGTAC700.0083432127.170783
CTAATAC700.0083432127.170783
CACTGCG700.00838393427.1436777
ACGCAGA30300.026.023897
CGCAGAG30300.026.023898
CGTAGAG1109.395621E-525.9357452
GCAGAGT32950.023.930929
Produced by FastQC (version 0.11.5)