##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937210_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401829 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74847758623693 33.0 31.0 34.0 30.0 34.0 2 31.837298950548618 33.0 31.0 34.0 30.0 34.0 3 31.876999917875516 33.0 31.0 34.0 30.0 34.0 4 35.498321425282896 37.0 35.0 37.0 33.0 37.0 5 35.50692956456602 37.0 35.0 37.0 33.0 37.0 6 35.559636064096914 37.0 35.0 37.0 33.0 37.0 7 35.508726348770246 37.0 35.0 37.0 33.0 37.0 8 35.49477265204851 37.0 35.0 37.0 33.0 37.0 9 37.1225148010721 39.0 37.0 39.0 34.0 39.0 10-11 37.00495111104475 39.0 37.0 39.0 33.0 39.0 12-13 36.86530091158179 39.0 37.0 39.0 33.0 39.0 14-15 38.04800051763312 40.0 38.0 41.0 33.0 41.0 16-17 38.01053806469916 40.0 38.0 41.0 33.0 41.0 18-19 38.00356246064868 40.0 38.0 41.0 33.0 41.0 20-21 37.95000360850013 40.0 38.0 41.0 33.0 41.0 22-23 37.807563665141146 40.0 38.0 41.0 33.0 41.0 24-25 37.64735372509202 40.0 37.0 41.0 32.0 41.0 26-27 37.48833085715566 40.0 37.0 41.0 32.0 41.0 28-29 37.519266902090195 40.0 37.0 41.0 32.0 41.0 30-31 37.33623630947493 40.0 37.0 41.0 32.0 41.0 32-33 37.123659566631574 39.5 36.0 41.0 31.5 41.0 34-35 36.98120718016868 39.0 36.0 41.0 31.0 41.0 36-37 36.9900716473923 40.0 36.0 41.0 31.0 41.0 38-39 36.88821115449606 40.0 36.0 41.0 31.0 41.0 40-41 36.69191248018436 39.5 36.0 41.0 30.5 41.0 42-43 36.44463565347449 39.0 35.0 41.0 30.0 41.0 44-45 36.17511677853017 39.0 35.0 41.0 29.5 41.0 46-47 35.78533654863138 39.0 35.0 41.0 28.0 41.0 48-49 35.4261203148603 39.0 35.0 41.0 26.5 41.0 50-51 34.50561432848301 38.0 34.0 39.5 25.0 40.5 52-53 34.580584029525 38.0 34.5 40.0 25.5 40.5 54-55 34.8820530623723 39.0 35.0 40.0 25.5 41.0 56-57 34.68220437051582 38.0 34.5 40.0 24.5 41.0 58-59 34.434533097412086 38.0 34.0 40.0 23.5 41.0 60-61 34.14244616491094 38.0 34.0 40.0 23.0 41.0 62-63 33.803138897391676 37.0 34.0 40.0 22.0 41.0 64-65 33.43078647882557 37.0 33.0 40.0 21.5 41.0 66-67 33.04912164129518 36.0 33.0 39.0 19.5 41.0 68-69 32.568722018570085 36.0 32.5 39.0 18.0 40.5 70-71 32.14012552603222 35.0 32.0 38.5 15.0 40.0 72-73 31.75132332410055 35.0 31.5 38.0 12.0 40.0 74-75 31.305674801968003 35.0 31.0 37.0 7.5 39.0 76-77 30.878908690014907 35.0 31.0 36.5 7.0 39.0 78-79 30.433251457709623 35.0 30.5 36.0 2.0 39.0 80-81 29.966905076537532 34.0 30.0 36.0 2.0 37.5 82-83 29.50317299149638 34.0 29.5 35.0 2.0 37.0 84-85 29.08727593080639 34.0 29.0 35.0 2.0 36.5 86-87 28.657462502706377 34.0 29.0 35.0 2.0 36.0 88-89 28.264306458717513 34.0 29.0 35.0 2.0 36.0 90-91 27.867077786819767 34.0 27.5 35.0 2.0 35.0 92-93 27.43394080566609 33.5 26.5 35.0 2.0 35.0 94-95 26.922353787307536 33.0 25.5 35.0 2.0 35.0 96-97 26.31902251952945 33.0 25.0 35.0 2.0 35.0 98-99 25.58455089105067 32.5 22.0 35.0 2.0 35.0 100-101 23.689481097680854 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02309681737654401 1101 2 -0.027500589560407462 1101 3 -0.13297445556614207 1101 4 -0.08248245555303413 1101 5 0.06068007967792255 1101 6 -0.11178725998783534 1101 7 -0.15765723307009694 1101 8 0.011364612471176372 1101 9 -0.02684018345792083 1101 10-11 -0.029721708177611106 1101 12-13 -0.023906497039185126 1101 14-15 -0.1919249918981194 1101 16-17 -0.08271651178036166 1101 18-19 -0.10065805192506616 1101 20-21 -0.0057441175160946045 1101 22-23 0.10976881253719029 1101 24-25 0.0988400776418743 1101 26-27 0.06062102207405218 1101 28-29 -0.11053619030140993 1101 30-31 -0.22631629967253986 1101 32-33 0.015696834344858246 1101 34-35 -0.01853616196530794 1101 36-37 0.2432904432033638 1101 38-39 -0.018232597694115782 1101 40-41 0.2372341404595204 1101 42-43 0.44357625756342856 1101 44-45 0.4078751533739293 1101 46-47 0.5083198807606237 1101 48-49 0.48183323585732296 1101 50-51 0.4596028692088936 1101 52-53 0.6834170932372174 1101 54-55 0.29960355162558727 1101 56-57 0.5763088532250009 1101 58-59 0.7700789485799504 1101 60-61 0.594480348097008 1101 62-63 0.7637832470743362 1101 64-65 0.8011673004053179 1101 66-67 0.45453512800020945 1101 68-69 0.6268732597959144 1101 70-71 0.7375701460811186 1101 72-73 0.8375329929791882 1101 74-75 0.5805248321146763 1101 76-77 0.8152973726447925 1101 78-79 0.5434404651223552 1101 80-81 0.42608710644381276 1101 82-83 0.5216494105614906 1101 84-85 0.7593596684133779 1101 86-87 0.7577946882370732 1101 88-89 0.5723727615538401 1101 90-91 0.6753205483849243 1101 92-93 0.9605714067915194 1101 94-95 0.8851837319451512 1101 96-97 0.7234340057885937 1101 98-99 0.7274080702019852 1101 100-101 1.0509482196483368 1102 1 0.051632355889214665 1102 2 0.08345284724668645 1102 3 0.09425392580858016 1102 4 0.0330322736202362 1102 5 0.19250776418853377 1102 6 -0.028765403795247835 1102 7 0.017319010198825424 1102 8 -0.11673801660705863 1102 9 -0.046447405504167705 1102 10-11 0.10164034440395397 1102 12-13 0.32962850454459414 1102 14-15 0.31081811864986975 1102 16-17 0.3479170010482733 1102 18-19 0.35500872032707775 1102 20-21 0.1277307003489696 1102 22-23 0.20699085878363377 1102 24-25 0.30588474029629964 1102 26-27 0.21983546616654337 1102 28-29 0.41528896015788774 1102 30-31 0.3643045428995251 1102 32-33 0.5372299353901511 1102 34-35 0.606452751560191 1102 36-37 0.22991381982674142 1102 38-39 0.10208415872020282 1102 40-41 0.3367621734021853 1102 42-43 0.48699722683806357 1102 44-45 0.39912950877462094 1102 46-47 0.47948871192945575 1102 48-49 0.6140441956292619 1102 50-51 0.8218613424429719 1102 52-53 0.6171231432847293 1102 54-55 0.3800026647076322 1102 56-57 0.14807952158103177 1102 58-59 0.3159262719882534 1102 60-61 0.3943853211727131 1102 62-63 0.1940018090002198 1102 64-65 0.568725108450927 1102 66-67 0.5382316753552985 1102 68-69 0.4818558381931197 1102 70-71 0.20693346568834414 1102 72-73 0.22901224543404552 1102 74-75 0.2665970525771435 1102 76-77 0.4326239485079526 1102 78-79 0.019145248144209148 1102 80-81 0.1547725673243967 1102 82-83 0.09558035766316664 1102 84-85 0.4422833301175295 1102 86-87 0.25871644940273697 1102 88-89 0.1279001609836783 1102 90-91 0.28027145114070606 1102 92-93 0.5110718819261422 1102 94-95 0.2275689077449634 1102 96-97 0.3341498752849539 1102 98-99 0.7463089254443069 1102 100-101 0.8744895563604054 1103 1 0.028947153767390432 1103 2 -0.04754770150033849 1103 3 -0.007293540809307331 1103 4 0.07764906527928161 1103 5 -0.019140306051085076 1103 6 -0.12344255762732814 1103 7 -0.2594141158300758 1103 8 0.017891579149292625 1103 9 -0.01253483919722953 1103 10-11 0.00990630379666868 1103 12-13 0.10282952887237684 1103 14-15 0.2141087716588288 1103 16-17 0.002195398706959395 1103 18-19 0.1505565527763153 1103 20-21 0.0373191400782531 1103 22-23 -0.13068611213277848 1103 24-25 -0.11877046365467692 1103 26-27 0.16732220478004223 1103 28-29 0.2301911002712984 1103 30-31 -0.08041651069476785 1103 32-33 -0.13395718734626882 1103 34-35 -0.331683337702664 1103 36-37 0.02095643917922274 1103 38-39 -0.23005229637400504 1103 40-41 -0.0049261382861303105 1103 42-43 -0.03371431514108991 1103 44-45 -0.20034735239237733 1103 46-47 -0.3779980266840468 1103 48-49 -0.7075837538860128 1103 50-51 -0.08615950994407484 1103 52-53 -0.11303233503976173 1103 54-55 -0.37277216609537334 1103 56-57 -0.25798962054444985 1103 58-59 -0.010384318829380845 1103 60-61 0.02095382309332905 1103 62-63 -0.3567938741898331 1103 64-65 -0.05974205818525036 1103 66-67 0.17704089430535674 1103 68-69 0.021030888494934175 1103 70-71 0.2002241186973137 1103 72-73 0.06692151925880907 1103 74-75 -0.15357488815817888 1103 76-77 -0.11605905862575838 1103 78-79 -0.19917375679451155 1103 80-81 -0.10519816616746525 1103 82-83 0.05124159783145288 1103 84-85 -0.09113353470778662 1103 86-87 -0.27100003129966055 1103 88-89 -0.20071699650671704 1103 90-91 -0.07860544660942992 1103 92-93 0.20170111185114692 1103 94-95 0.3432246604429672 1103 96-97 0.24869532983040799 1103 98-99 0.6638560262308424 1103 100-101 1.1645444310631667 1104 1 0.1391111196701651 1104 2 0.20168527088666366 1104 3 0.12193511142217162 1104 4 0.045155895525169853 1104 5 0.2991551525854277 1104 6 0.0920732009399785 1104 7 0.12736780831919958 1104 8 0.05730499803237166 1104 9 0.2665112064604074 1104 10-11 0.04733165110916815 1104 12-13 0.2335522348897925 1104 14-15 0.29449966573101705 1104 16-17 0.2375898729080248 1104 18-19 0.31276039211452655 1104 20-21 0.39047785379194977 1104 22-23 0.2668281983823988 1104 24-25 0.5876649680208601 1104 26-27 0.3809758963129397 1104 28-29 0.32915666303156144 1104 30-31 0.21886626348510418 1104 32-33 0.39921800696073007 1104 34-35 0.6351514683503581 1104 36-37 0.5111120847824608 1104 38-39 0.18491264236379834 1104 40-41 0.4039065796917214 1104 42-43 0.39847924382703326 1104 44-45 0.5779366658322829 1104 46-47 0.4660023573297778 1104 48-49 0.4983456595728697 1104 50-51 0.26043896760111807 1104 52-53 0.2776617299465798 1104 54-55 0.6813617827267819 1104 56-57 0.7248877630413659 1104 58-59 0.5604482311424164 1104 60-61 0.7271252452645243 1104 62-63 0.9425469020781136 1104 64-65 0.7084015949862632 1104 66-67 0.9171635387205583 1104 68-69 0.7990128958472056 1104 70-71 0.8979112354335115 1104 72-73 0.9974290175287486 1104 74-75 0.6147047699095118 1104 76-77 0.7546282861186526 1104 78-79 0.6448546282273497 1104 80-81 0.7640731275991115 1104 82-83 0.6708379032984482 1104 84-85 0.8359558405097189 1104 86-87 0.8993201002250757 1104 88-89 0.6645981548387319 1104 90-91 0.8517480461163061 1104 92-93 1.0938172280313267 1104 94-95 0.5555483040941382 1104 96-97 0.7168861847011847 1104 98-99 0.863473212449545 1104 100-101 0.8774300949470693 1105 1 0.06564167989853686 1105 2 0.22468827848211959 1105 3 0.4909050524597056 1105 4 0.28312551371347183 1105 5 0.09838966007043126 1105 6 0.3093464843166416 1105 7 0.25768420056402164 1105 8 0.3006823408132959 1105 9 0.27185096279420407 1105 10-11 0.3343637321273363 1105 12-13 0.1130317679478523 1105 14-15 0.2462804341121867 1105 16-17 0.23716719529846841 1105 18-19 0.30633739165574525 1105 20-21 0.3884494260676945 1105 22-23 0.213494355287132 1105 24-25 0.6019298063413672 1105 26-27 0.7399481312792062 1105 28-29 0.6379152227841516 1105 30-31 0.57462699822198 1105 32-33 0.33205899521920657 1105 34-35 0.7405320056394373 1105 36-37 0.46383378874670456 1105 38-39 0.6304913071273504 1105 40-41 0.48255859919861166 1105 42-43 0.36517904501987886 1105 44-45 0.31609532074043756 1105 46-47 0.1487350211757601 1105 48-49 0.31257566416072535 1105 50-51 0.03338037896200774 1105 52-53 -0.17715424599265361 1105 54-55 0.272294816999775 1105 56-57 0.13989770339921392 1105 58-59 0.5944111204730973 1105 60-61 0.23768368947108343 1105 62-63 0.1674283824267917 1105 64-65 0.41322394294976306 1105 66-67 0.09792087504449398 1105 68-69 0.16920626554355067 1105 70-71 0.21209663585151617 1105 72-73 0.4387830302048279 1105 74-75 0.14916892514901647 1105 76-77 0.18105052193452664 1105 78-79 -0.031204402205439408 1105 80-81 0.34884016639199444 1105 82-83 0.30389426597707825 1105 84-85 0.20998729282148787 1105 86-87 0.3109231316094174 1105 88-89 0.06589549897901392 1105 90-91 0.13825124912050413 1105 92-93 0.1809397917940494 1105 94-95 0.5415277267037801 1105 96-97 0.41749874863382885 1105 98-99 0.5640284281638088 1105 100-101 1.3095361764070255 1106 1 -0.20018804864512774 1106 2 -0.14292190269774352 1106 3 -0.06523040404249869 1106 4 0.0997671035824439 1106 5 -0.05704991529579928 1106 6 -0.09882541713154325 1106 7 0.029699539205275016 1106 8 -0.08096562274175767 1106 9 0.005519698296680531 1106 10-11 -0.2332051768229917 1106 12-13 -0.5126186614923043 1106 14-15 -0.3288506966424194 1106 16-17 -0.09947575289704957 1106 18-19 -0.06981457373827027 1106 20-21 0.0017782664747727495 1106 22-23 -0.03434277091185578 1106 24-25 -0.25473539751209984 1106 26-27 -0.33478333801216564 1106 28-29 -0.33648032190479427 1106 30-31 -0.33610221415759867 1106 32-33 -0.09665759517219641 1106 34-35 -0.5297175084975976 1106 36-37 -0.4184280339185378 1106 38-39 0.05315550790891166 1106 40-41 -0.1600082200185753 1106 42-43 -0.33958868114148544 1106 44-45 -0.12453797288796409 1106 46-47 0.0642053156191622 1106 48-49 0.028251797318525007 1106 50-51 0.4448775682709396 1106 52-53 -0.04240564088952681 1106 54-55 0.13611735686144044 1106 56-57 0.254200881883321 1106 58-59 0.3134412753223259 1106 60-61 0.22104680150167866 1106 62-63 0.027876003265610905 1106 64-65 0.014377475643641446 1106 66-67 0.2993763741772568 1106 68-69 0.24980204846207243 1106 70-71 0.42621330565278726 1106 72-73 0.20221740213553474 1106 74-75 0.0802756478205282 1106 76-77 0.3834908078100199 1106 78-79 0.015955650455833137 1106 80-81 0.3086467615897881 1106 82-83 0.11529584999480136 1106 84-85 0.09522176087992307 1106 86-87 0.14055241295018917 1106 88-89 0.07992283268850287 1106 90-91 0.2322474458061876 1106 92-93 0.18677537159051028 1106 94-95 0.27505279305280794 1106 96-97 0.15945327604067572 1106 98-99 0.6602475784783159 1106 100-101 1.1310599053268433 1107 1 0.02306032467216923 1107 2 -0.08468467648248179 1107 3 -0.05374860530299941 1107 4 -0.0922240014272262 1107 5 -0.2955411706353459 1107 6 -0.06183859510509393 1107 7 -0.055387169931947255 1107 8 0.07572348940432505 1107 9 0.41214782756902935 1107 10-11 -0.04595088107590328 1107 12-13 -0.03357124234424447 1107 14-15 0.1484409830244715 1107 16-17 -0.0359429441109711 1107 18-19 0.10653371505392784 1107 20-21 -0.0694724213553144 1107 22-23 -0.013393026472648728 1107 24-25 0.07564428469621731 1107 26-27 0.034733167073525806 1107 28-29 0.013076346785183546 1107 30-31 0.22948699385923277 1107 32-33 0.12576821845279795 1107 34-35 -0.3745997690050942 1107 36-37 -0.36890710402898463 1107 38-39 -0.20337249599399598 1107 40-41 -0.37400137859106763 1107 42-43 -0.22124786913325778 1107 44-45 -0.5118217673274614 1107 46-47 -0.06675469663414191 1107 48-49 -0.07743861390459017 1107 50-51 -0.15513718107527552 1107 52-53 -0.15760370759061004 1107 54-55 -0.04567968078910667 1107 56-57 -0.3827241063455631 1107 58-59 -0.00903048818892671 1107 60-61 0.35100839647328286 1107 62-63 0.06257384021726153 1107 64-65 0.009541391935428578 1107 66-67 0.27425993943460725 1107 68-69 0.04638020014208166 1107 70-71 0.4048579541928845 1107 72-73 0.1260909208189851 1107 74-75 0.22370736685683212 1107 76-77 0.2261379750846224 1107 78-79 0.2834038433772861 1107 80-81 0.6612100277757769 1107 82-83 0.8488602564187069 1107 84-85 0.8437028618618854 1107 86-87 0.7257951247876768 1107 88-89 0.90650172183582 1107 90-91 0.7331210609276724 1107 92-93 0.7003589521644891 1107 94-95 1.0931394624802486 1107 96-97 0.6732892973478108 1107 98-99 0.913317995260801 1107 100-101 0.828357938558252 1108 1 0.09362792129941155 1108 2 0.0822555080005678 1108 3 -0.1595819943688035 1108 4 -0.014063070060473137 1108 5 -0.11622837771834327 1108 6 -0.02388735501475736 1108 7 -0.015275224834432777 1108 8 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2316 66-67 -1.3524518516440196 2316 68-69 -1.421677996393342 2316 70-71 -1.6543242239903968 2316 72-73 -2.064004387056272 2316 74-75 -1.8726966361902306 2316 76-77 -1.9893514445200715 2316 78-79 -2.0887039202312714 2316 80-81 -2.015749191618415 2316 82-83 -2.4965362976113816 2316 84-85 -2.7648777544119767 2316 86-87 -2.496881636721664 2316 88-89 -2.3577115956807084 2316 90-91 -2.4122917751067305 2316 92-93 -2.878905333840546 2316 94-95 -2.8745204064496157 2316 96-97 -2.5332595573876375 2316 98-99 -2.8803919175723287 2316 100-101 -3.283653528624786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1741.0 3 355.0 4 340.0 5 341.0 6 399.0 7 495.0 8 525.0 9 648.0 10 798.0 11 1073.0 12 1348.0 13 1762.0 14 1983.0 15 2759.0 16 3594.0 17 4642.0 18 4004.0 19 2765.0 20 2520.0 21 2621.0 22 2666.0 23 3144.0 24 3505.0 25 3830.0 26 4379.0 27 5088.0 28 6020.0 29 7398.0 30 9033.0 31 11303.0 32 14306.0 33 18997.0 34 26145.0 35 38114.0 36 57169.0 37 81707.0 38 65517.0 39 8794.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.891535982867794 19.111491604871098 14.701147388362381 22.295825023898725 2 15.33943617342445 20.24191797842798 40.74207142269029 23.676574425457275 3 19.249433033030037 24.904632288550633 29.85552010019535 25.990414578223977 4 12.320229148577303 16.42040180843738 37.04477179955488 34.21459724343045 5 13.140350702102815 38.14484176488787 33.96266429804689 14.752143234962425 6 31.652573754017787 36.42355041655342 17.272815681658933 14.651060147769861 7 28.298490344939353 33.54849969457845 20.134385479387163 18.01862448109503 8 26.860223311574003 35.21171712537463 19.231824143382187 18.696235419669172 9 26.596322909758204 16.579568064067338 19.991123921032816 36.83298510514164 10-11 25.042798237946982 27.35596039495324 26.61969354840721 20.981547818692576 12-13 26.01186698700965 24.672311319519686 27.653022166753733 21.66279952671693 14-15 22.607735137251186 25.87974656716195 26.266727838963927 25.24579045662294 16-17 21.612072340287597 29.531802847033656 26.8111876603607 22.044937152318045 18-19 21.707351432350592 27.242025424394406 30.08914869293879 20.961474450316214 20-21 22.326730019584303 26.443921697688655 30.342955945911914 20.886392336815128 22-23 21.92034247574359 26.54162080086367 30.204610307931468 21.333426415461272 24-25 21.661736603753827 26.77965298839098 30.3598384983689 21.19877190948629 26-27 21.145964219469874 27.12534155048964 30.23719061010391 21.491503619936577 28-29 21.318775572283073 27.257529253071166 30.25514312998511 21.168552044660643 30-31 21.36730292111858 27.107057811467538 30.039646423718036 21.485992843695843 32-33 21.024272873474377 27.155323949983167 30.11980601648112 21.700597160061335 34-35 21.587715601614846 27.343409080141335 29.882751621453814 21.18612369679001 36-37 21.642001286455816 27.106322082662267 29.856495355296158 21.395181275585763 38-39 21.292533458324193 27.39518718710733 29.957541336737343 21.354738017831142 40-41 21.58736767573012 27.386199881325762 29.865519149933434 21.160913293010687 42-43 21.350051485859463 27.4854705159307 29.523213515474428 21.64126448273541 44-45 21.412587499766254 27.743861770627504 29.474346907354654 21.36920382225159 46-47 21.73120053058268 27.9927244348534 28.77376308535824 21.502311949205684 48-49 21.756877216823234 28.220370151040076 28.433434525405026 21.58931810673166 50-51 21.739585774914097 28.415636423481068 28.370254190375977 21.47452361122886 52-53 22.099882911719927 28.255185115448544 28.251818359791457 21.393113613040068 54-55 21.620762394901412 28.535576433978637 28.307551140500486 21.536110030619472 56-57 21.575304899330867 28.759137007748382 28.1047128511453 21.560845241775446 58-59 21.663043424063982 28.32960205826772 28.282358631212073 21.72499588645622 60-61 21.472901939911722 28.42120877079924 28.640466286819745 21.465423002469297 62-63 21.493243976050053 28.397116332761136 28.726721043964332 21.38291864722448 64-65 21.325041695024268 28.47240400172514 28.56738638884942 21.635167914401173 66-67 21.23412747382054 28.648016643170283 28.384757388896993 21.73309849411219 68-69 21.442175378417723 28.476930977404624 28.47020022734978 21.61069341682787 70-71 21.31061099019454 28.54466309152512 28.214199345305317 21.93052657297502 72-73 21.401254035726307 28.59562957330375 28.082546964011918 21.92056942695803 74-75 21.279237478823358 28.746380181031462 28.065985108099046 21.908397232046134 76-77 21.41196504057034 28.6992933069222 28.081606907117667 21.807134745389792 78-79 21.486457680172627 28.677032469904223 27.86899346662694 21.967516383296207 80-81 21.422952789720973 28.891877977054943 27.8787599245553 21.806409308668783 82-83 21.356327283605903 28.86006681292381 27.899880335061827 21.883725568408458 84-85 21.341285570055714 28.762791509304613 27.79418913360506 22.101733787034615 86-87 21.481679993518352 28.792730581541008 27.62989785170735 22.095691573233285 88-89 21.4168635052009 28.614360591575107 27.64822097436633 22.32055492885767 90-91 21.144127905382327 28.526161218023034 28.13487106995854 22.194839806636104 92-93 21.231815501899003 28.338462573993816 28.14426191909636 22.285460005010826 94-95 21.071275881023638 28.256450078962754 28.199611854758395 22.472662185255217 96-97 21.007458593835693 28.127524006102483 28.23371922582189 22.63129817423993 98-99 20.990561333878478 28.633362917460286 27.79644791033018 22.579627838331056 100-101 20.93607325855749 28.530653401619578 27.98123914850762 22.552034191315315 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 720.0 1 428.5 2 168.0 3 213.5 4 192.5 5 186.0 6 301.0 7 579.0 8 915.5 9 1045.5 10 920.5 11 769.5 12 754.0 13 799.0 14 836.5 15 877.0 16 905.5 17 929.0 18 994.5 19 1087.0 20 1156.5 21 1276.5 22 1519.5 23 1755.5 24 2017.5 25 2407.0 26 2937.0 27 3577.5 28 4099.5 29 4721.5 30 5404.5 31 6107.5 32 7173.5 33 7834.0 34 8247.0 35 8814.5 36 9483.0 37 10221.0 38 10896.0 39 11388.0 40 11773.5 41 12413.5 42 12818.5 43 13167.0 44 13753.0 45 14294.5 46 14368.0 47 14392.0 48 14872.5 49 14699.0 50 14239.5 51 13763.5 52 13196.0 53 13115.5 54 12890.0 55 11952.5 56 10665.5 57 9504.0 58 8601.0 59 7497.0 60 6138.0 61 5027.0 62 3933.5 63 2865.5 64 2085.0 65 1506.0 66 1036.5 67 791.0 68 607.5 69 445.0 70 324.0 71 212.5 72 144.0 73 122.0 74 95.0 75 68.0 76 48.5 77 27.0 78 16.0 79 12.0 80 7.5 81 6.5 82 4.5 83 2.5 84 1.5 85 3.5 86 4.5 87 1.5 88 1.0 89 1.0 90 2.5 91 3.5 92 1.5 93 0.5 94 1.0 95 1.5 96 1.0 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2936572522142503 2 0.2568256646483952 3 0.2518484230854418 4 0.2590654233517242 5 0.25707452672654285 6 0.19908966251813584 7 0.18515338614186633 8 0.19286811056444406 9 0.1868954206889 10-11 0.20394247304201538 12-13 0.19921409355720968 14-15 0.19311697264259173 16-17 0.1941124209551824 18-19 0.18851302419685986 20-21 0.18478009302464482 22-23 0.1865221275716785 24-25 0.17880740314910074 26-27 0.17843411003187923 28-29 0.18913517939222904 30-31 0.1951078692677731 32-33 0.1901306277048197 34-35 0.19908966251813584 36-37 0.18092273081335594 38-39 0.18241590328224197 40-41 0.18191817912594663 42-43 0.18614883445445699 44-45 0.1882641621187122 46-47 0.18925961043130288 48-49 0.19324140368166556 50-51 0.1968499038148068 52-53 0.2114083353864455 54-55 0.1933658347207394 56-57 0.17743866171928854 58-59 0.17793638587558389 60-61 0.17482560989873802 62-63 0.18478009302464482 64-65 0.17470117885966419 66-67 0.1750744719768857 68-69 0.1704705235311538 70-71 0.18067386873520827 72-73 0.18017614457891293 74-75 0.18378464471205413 76-77 0.18303805847761112 78-79 0.18278919639946345 80-81 0.18428236886834948 82-83 0.17644321340669786 84-85 0.17022166145300613 86-87 0.173332437429852 88-89 0.173332437429852 90-91 0.17918069626632224 92-93 0.17358129950799966 94-95 0.171839264960966 96-97 0.16947507521856311 98-99 0.17743866171928854 100-101 0.16785747171060328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 401829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.62030276470168 #Duplication Level Percentage of deduplicated Percentage of total 1 87.4316092620999 65.24173154342989 2 8.012123929923687 11.957342268784338 3 2.0012137907321863 4.4799353688399615 4 0.8794982378178822 2.6251369914796783 5 0.4564007622241308 1.7028381529602628 6 0.2928589657515583 1.3111934815043207 7 0.19910566535758406 1.040012752180521 8 0.13685090192122437 0.8169484587987405 9 0.09871546988911903 0.6629560425617267 >10 0.457557936131758 5.89940964783683 >50 0.01937033856954607 1.0048164775272284 >100 0.013024882810846138 1.6106470452498176 >500 6.679427082485198E-4 0.2614217469390467 >1k 0.0010019140623727797 1.385610021907626 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2409 0.5995087462577365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1870 0.4653720861361425 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1281 0.31879232210716496 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 548 0.13637641882492305 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 501 0.12467990115198255 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 2.488620781476698E-4 0.0 0.0 0.0 1.244310390738349E-4 16-17 2.488620781476698E-4 0.0 0.0 1.244310390738349E-4 2.488620781476698E-4 18-19 2.488620781476698E-4 0.0 0.0 2.488620781476698E-4 2.488620781476698E-4 20-21 2.488620781476698E-4 0.0 0.0 2.488620781476698E-4 2.488620781476698E-4 22-23 2.488620781476698E-4 0.0 0.0 7.465862344430093E-4 2.488620781476698E-4 24-25 2.488620781476698E-4 0.0 0.0 0.001244310390738349 2.488620781476698E-4 26-27 2.488620781476698E-4 0.0 0.0 0.001244310390738349 2.488620781476698E-4 28-29 2.488620781476698E-4 0.0 0.0 0.003110775976845872 2.488620781476698E-4 30-31 2.488620781476698E-4 0.0 0.0 0.005723827797396405 3.7329311722150466E-4 32-33 2.488620781476698E-4 0.0 0.0 0.010576638321275965 4.977241562953396E-4 34-35 2.488620781476698E-4 0.0 0.0 0.014682862610712517 4.977241562953396E-4 36-37 2.488620781476698E-4 0.0 0.0 0.020904414564404263 4.977241562953396E-4 38-39 2.488620781476698E-4 0.0 0.0 0.026503811322726833 4.977241562953396E-4 40-41 2.488620781476698E-4 0.0 0.0 0.033969673667156926 4.977241562953396E-4 42-43 3.7329311722150466E-4 0.0 0.0 0.050021277707681625 4.977241562953396E-4 44-45 7.465862344430093E-4 0.0 0.0 0.06930808876412603 4.977241562953396E-4 46-47 7.465862344430093E-4 0.0 0.0 0.08921705501593963 4.977241562953396E-4 48-49 7.465862344430093E-4 0.0 0.0 0.11572086633866645 4.977241562953396E-4 50-51 7.465862344430093E-4 0.0 0.0 0.15031269520119256 4.977241562953396E-4 52-53 7.465862344430093E-4 0.0 0.0 0.21377252512884834 4.977241562953396E-4 54-55 7.465862344430093E-4 0.0 0.0 0.26304721660208696 4.977241562953396E-4 56-57 7.465862344430093E-4 0.0 0.0 0.3577392373372753 4.977241562953396E-4 58-59 7.465862344430093E-4 0.0 0.0 0.45678634444004784 4.977241562953396E-4 60-61 7.465862344430093E-4 0.0 0.0 0.5625527276528075 4.977241562953396E-4 62-63 7.465862344430093E-4 0.0 0.0 0.7165983540262151 7.465862344430093E-4 64-65 7.465862344430093E-4 0.0 0.0 0.796483081111617 7.465862344430093E-4 66-67 9.954483125906792E-4 0.0 0.0 0.9966926229814175 7.465862344430093E-4 68-69 9.954483125906792E-4 0.0 0.0 1.2319917178700392 7.465862344430093E-4 70-71 9.954483125906792E-4 0.0 0.0 1.4275973112941078 7.465862344430093E-4 72-73 9.954483125906792E-4 0.0 0.0 1.7625656684808713 7.465862344430093E-4 74-75 9.954483125906792E-4 0.0 0.0 2.079242662923781 7.465862344430093E-4 76-77 9.954483125906792E-4 0.0 0.0 2.2826874118095013 7.465862344430093E-4 78-79 9.954483125906792E-4 0.0 0.0 2.521719437870338 7.465862344430093E-4 80-81 9.954483125906792E-4 0.0 0.0 2.9594678333320887 7.465862344430093E-4 82-83 9.954483125906792E-4 0.0 0.0 3.3250462261310156 7.465862344430093E-4 84-85 9.954483125906792E-4 0.0 0.0 3.831480555161524 7.465862344430093E-4 86-87 9.954483125906792E-4 0.0 0.0 4.491960510565439 7.465862344430093E-4 88-89 9.954483125906792E-4 0.0 0.0 5.388610578131494 7.465862344430093E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1100 0.0 54.471863 1 GTATCAA 1985 0.0 40.966606 1 TATCAAC 2475 0.0 32.851944 2 GTGGTAT 490 0.0 32.026703 1 ATCAACG 2545 0.0 31.94835 3 TCAACGC 2580 0.0 31.330647 4 CAACGCA 2655 0.0 30.445602 5 TGGTATC 485 0.0 30.392057 2 AACGCAG 2755 0.0 29.311232 6 TGTATCG 65 0.005810322 29.26084 5 CTAGTAA 65 0.005810322 29.26084 4 GGTCTAG 100 4.9064503E-5 28.532879 1 TAGGGTC 105 6.846706E-5 27.17078 5 GTAGTAC 70 0.00834321 27.17078 3 CTAATAC 70 0.00834321 27.17078 3 CACTGCG 70 0.008383934 27.143677 7 ACGCAGA 3030 0.0 26.02389 7 CGCAGAG 3030 0.0 26.02389 8 CGTAGAG 110 9.395621E-5 25.935745 2 GCAGAGT 3295 0.0 23.93092 9 >>END_MODULE